FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_50330665-1.342000000356cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_50330665-1.342000000356cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2801657
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA210730.7521620241164425No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC195410.6974800983846345No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT135870.48496300582119795Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC111660.3985498581732168No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT95900.3422974332689548No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT94600.33765732207761334No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75360.2689836764457605No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT65830.23496809209692693No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT57220.2042362787450427No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC44150.15758531469055634No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA40960.14619919569026474No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT39820.14213017510708842No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA34750.12403374146085691No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC30980.11057741900596682No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC29790.10632993260773893No Hit
TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA29100.10386710436002694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC40800.089.600569
GTTCGGA10350.087.9836042
ATTCGGA9550.086.911162
AAGCGGC14750.086.7937249
CGGGAGA53400.086.238924
AGAGCGG45350.083.22778
ATCCGGA11600.083.000162
TCGGAGA15350.082.8040854
ACCGGAA7200.082.338483
GTCCGGA5550.082.038772
ATCGGGA68350.080.7015842
GGATCGG26200.080.567851
CGGAAAG30850.080.402925
CGGAGAG26550.079.1342545
TCGGGAG59950.078.9526443
TCCGGAA9400.077.1949543
TTGGGAG33200.075.997433
AAGGGGC45350.075.8014149
AACCGGA6300.073.777922
GGAGAGC56150.072.8086246
Produced by FastQC (version 0.11.2)