##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3314319 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22921450831981 33.0 33.0 33.0 33.0 33.0 2 32.251328251746436 33.0 33.0 33.0 33.0 33.0 3 32.26116134264686 33.0 33.0 33.0 33.0 33.0 4 36.05107263362398 37.0 37.0 37.0 33.0 37.0 5 36.02797407250177 37.0 37.0 37.0 33.0 37.0 6 35.95030683528049 37.0 37.0 37.0 33.0 37.0 7 35.93940233272657 37.0 37.0 37.0 33.0 37.0 8 35.84854415039711 37.0 37.0 37.0 33.0 37.0 9 35.81360967366147 37.0 37.0 37.0 33.0 37.0 10-11 35.727282286346004 37.0 37.0 37.0 33.0 37.0 12-13 35.78272866914742 37.0 37.0 37.0 33.0 37.0 14-15 37.575096573383554 40.0 37.0 40.0 33.0 40.0 16-17 37.36932323050377 40.0 37.0 40.0 33.0 40.0 18-19 37.1075674972747 40.0 37.0 40.0 33.0 40.0 20-21 36.99752483089286 40.0 37.0 40.0 33.0 40.0 22-23 36.88714846096589 40.0 37.0 40.0 33.0 40.0 24-25 36.68513923976539 40.0 37.0 40.0 33.0 40.0 26-27 36.58556192086519 37.0 37.0 40.0 33.0 40.0 28-29 36.45024573675617 37.0 37.0 40.0 33.0 40.0 30-31 36.465270391896496 37.0 37.0 40.0 33.0 40.0 32-33 36.372716084360015 37.0 37.0 40.0 33.0 40.0 34-35 36.23634176432624 37.0 37.0 40.0 33.0 40.0 36-37 36.15552078722658 37.0 37.0 40.0 33.0 40.0 38-39 36.08008900169236 37.0 37.0 40.0 33.0 40.0 40-41 35.93836727846656 37.0 37.0 40.0 30.0 40.0 42-43 35.818714191361785 37.0 37.0 40.0 27.0 40.0 44-45 35.71081193451808 37.0 37.0 40.0 27.0 40.0 46-47 35.566800449805825 37.0 37.0 40.0 27.0 40.0 48-49 35.392671164121495 37.0 37.0 40.0 27.0 40.0 50-51 34.108368566815685 37.0 35.0 38.5 24.5 38.5 52-53 34.591622140174195 37.0 35.0 38.5 27.0 40.0 54-55 35.33175457763721 37.0 37.0 40.0 27.0 40.0 56-57 35.303844319149725 37.0 37.0 40.0 27.0 40.0 58-59 35.141987086940034 37.0 37.0 40.0 27.0 40.0 60-61 34.870258264216574 37.0 35.0 40.0 27.0 40.0 62-63 34.59172306588473 37.0 33.0 40.0 27.0 40.0 64-65 34.31277511307753 37.0 33.0 37.0 27.0 40.0 66-67 34.03079229850838 37.0 33.0 37.0 27.0 40.0 68-69 33.778308605779955 37.0 33.0 37.0 22.0 40.0 70-71 33.48641380024071 37.0 33.0 37.0 22.0 40.0 72-73 33.1905014272917 37.0 33.0 37.0 22.0 38.5 74-75 32.87297315074379 37.0 33.0 37.0 22.0 37.0 76-77 32.61115224575546 37.0 33.0 37.0 22.0 37.0 78-79 32.359140595700055 37.0 33.0 37.0 22.0 37.0 80-81 32.10642080620484 37.0 33.0 37.0 22.0 37.0 82-83 31.864102097595314 37.0 33.0 37.0 15.0 37.0 84-85 31.65123800696312 37.0 33.0 37.0 15.0 37.0 86-87 31.39673700690851 33.0 33.0 37.0 15.0 37.0 88-89 31.221071960785913 33.0 33.0 37.0 15.0 37.0 90-91 31.053356511548827 33.0 33.0 37.0 15.0 37.0 92-93 30.835404648737793 33.0 33.0 37.0 15.0 37.0 94-95 30.64928602225676 33.0 27.0 37.0 15.0 37.0 96-97 30.42674045558077 33.0 27.0 37.0 15.0 37.0 98-99 30.23596416035994 33.0 27.0 37.0 15.0 37.0 100-101 28.969054578029453 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1228732587131347 2306 2 0.12306375545225734 2306 3 0.1559802088548068 2306 4 0.18925977989893994 2306 5 0.24729424649871135 2306 6 0.20559416602475977 2306 7 0.22598573723185922 2306 8 0.23446562468458865 2306 9 0.32198039988294624 2306 10-11 0.24488275240820911 2306 12-13 0.17196067288356431 2306 14-15 0.4351065001523864 2306 16-17 0.5017890159692158 2306 18-19 0.6988048537441358 2306 20-21 0.6866648592307953 2306 22-23 0.6248494737532795 2306 24-25 0.6293509059859588 2306 26-27 0.7475279992139505 2306 28-29 0.7253273057019598 2306 30-31 0.8081679147158383 2306 32-33 0.811044110869652 2306 34-35 0.8733615735775473 2306 36-37 0.9506456730684576 2306 38-39 0.8046236688966175 2306 40-41 0.7406231409169521 2306 42-43 0.7473461587845662 2306 44-45 0.8632027662741777 2306 46-47 0.8389718547870402 2306 48-49 0.9103880146757746 2306 50-51 0.8877242298107788 2306 52-53 0.9684068420716443 2306 54-55 0.993917203075533 2306 56-57 1.1931082121007677 2306 58-59 1.0963849010982756 2306 60-61 0.9383472598203113 2306 62-63 1.0439422211192095 2306 64-65 0.9972036794212258 2306 66-67 1.0701681803051528 2306 68-69 1.0271074812188346 2306 70-71 0.9668407445463458 2306 72-73 1.0742912475948927 2306 74-75 1.1159875084093613 2306 76-77 1.0478621075683705 2306 78-79 1.134382235375739 2306 80-81 1.00560339501245 2306 82-83 1.0769836903663546 2306 84-85 1.268111669626439 2306 86-87 1.4856938749024629 2306 88-89 1.1951064861886458 2306 90-91 1.3115212037136779 2306 92-93 1.305191107186186 2306 94-95 1.4485909126053649 2306 96-97 1.5797249511764413 2306 98-99 1.4034575651660148 2306 100-101 1.271831042249154 2307 1 0.1642803561871773 2307 2 0.17794566172475612 2307 3 0.1923481544523895 2307 4 0.16649790899529648 2307 5 0.21929085546307903 2307 6 0.260352098951536 2307 7 0.17860786465093526 2307 8 0.19736113156237423 2307 9 0.2162785375480567 2307 10-11 0.20149116983375137 2307 12-13 0.1309883936882983 2307 14-15 0.14560412551588087 2307 16-17 0.3786050887488628 2307 18-19 0.47693723175345326 2307 20-21 0.5533442878595238 2307 22-23 0.5334036346086961 2307 24-25 0.48806778185764443 2307 26-27 0.6135720644458047 2307 28-29 0.35759957304683354 2307 30-31 0.5315979654902208 2307 32-33 0.6658995816493132 2307 34-35 0.6009884867324047 2307 36-37 0.5623693583584739 2307 38-39 0.6073592866211612 2307 40-41 0.6807239604172253 2307 42-43 0.7179043123109707 2307 44-45 0.524507585737993 2307 46-47 0.7056795420460418 2307 48-49 0.6117061430428876 2307 50-51 0.3715447966241996 2307 52-53 0.5011722680932849 2307 54-55 0.5996245348227305 2307 56-57 0.725178111190111 2307 58-59 0.7664307530048191 2307 60-61 0.8126127633252906 2307 62-63 0.8900502784751154 2307 64-65 0.8448358796746405 2307 66-67 0.8426342471596016 2307 68-69 0.7103255011092671 2307 70-71 0.7644105023425354 2307 72-73 0.6964423674570597 2307 74-75 0.7671310713668476 2307 76-77 0.7903047471067524 2307 78-79 0.6157342740991822 2307 80-81 0.8408665193230718 2307 82-83 0.6875451255401401 2307 84-85 0.9139116167554526 2307 86-87 0.9028559791676933 2307 88-89 1.012493747927369 2307 90-91 0.9099961492474442 2307 92-93 0.9906078308258977 2307 94-95 1.0218773913065569 2307 96-97 1.050841896327352 2307 98-99 0.9970902576665388 2307 100-101 0.9779813963935986 2201 1 0.13720182537420555 2201 2 0.13459867793354618 2201 3 0.15926884619612025 2201 4 0.20910541765113777 2201 5 0.17060753704580378 2201 6 0.2552408612798871 2201 7 0.13923307370182414 2201 8 0.23154061248337143 2201 9 0.242555216961172 2201 10-11 0.18374376658476166 2201 12-13 0.10094111428783492 2201 14-15 0.14700668746274204 2201 16-17 0.2820041431608544 2201 18-19 0.3645098910615303 2201 20-21 0.51148213036398 2201 22-23 0.36387850782215736 2201 24-25 0.39339281643579227 2201 26-27 0.38375847003616315 2201 28-29 0.33083473156390397 2201 30-31 0.49450216820403625 2201 32-33 0.5077933792790148 2201 34-35 0.5588677841406238 2201 36-37 0.5257382190424451 2201 38-39 0.47267220754794437 2201 40-41 0.431167826299216 2201 42-43 0.43129215707543267 2201 44-45 0.5341510466262633 2201 46-47 0.6412421488314948 2201 48-49 0.6015605080253934 2201 50-51 0.5001244258018289 2201 52-53 0.4897298395773859 2201 54-55 0.6427414496384571 2201 56-57 0.7140222518892685 2201 58-59 0.6883890061078191 2201 60-61 0.7736077099438674 2201 62-63 0.6897189610703265 2201 64-65 0.7495904878341761 2201 66-67 0.6525742213050947 2201 68-69 0.5795789747439954 2201 70-71 0.5697350849187686 2201 72-73 0.636856762814098 2201 74-75 0.5813790757783863 2201 76-77 0.7379385170739354 2201 78-79 0.6785675125121102 2201 80-81 0.6462747222538567 2201 82-83 0.6906096985339332 2201 84-85 0.7435502864234174 2201 86-87 0.7354453173464925 2201 88-89 0.8439598613436878 2201 90-91 0.8891145647095264 2201 92-93 0.866897618294594 2201 94-95 0.964853733526251 2201 96-97 1.037863113297309 2201 98-99 1.0588337194588142 2201 100-101 0.9090841002690659 1101 1 0.17014577751651672 1101 2 0.11627330223554111 1101 3 0.060711092190707916 1101 4 0.2145387660112945 1101 5 0.20314017102858628 1101 6 0.20887740739194527 1101 7 0.22291098196799908 1101 8 0.21364761443467017 1101 9 0.19325396425058727 1101 10-11 0.15665073299816612 1101 12-13 0.17263765362136496 1101 14-15 0.14485006408867918 1101 16-17 0.27506931899113596 1101 18-19 0.3743198291663674 1101 20-21 0.39005245425234847 1101 22-23 0.38370322047529015 1101 24-25 0.23270167030596411 1101 26-27 0.41658856931191934 1101 28-29 0.38029294328416796 1101 30-31 0.335725132261409 1101 32-33 0.46739025457721795 1101 34-35 0.4151474607458354 1101 36-37 0.543697850632995 1101 38-39 0.5602374014762361 1101 40-41 0.5658559319652099 1101 42-43 0.49643442380045144 1101 44-45 0.5538464408500445 1101 46-47 0.5205253693539689 1101 48-49 0.5533833055811712 1101 50-51 0.6465372378689196 1101 52-53 0.6704674811119631 1101 54-55 0.6561203131499269 1101 56-57 0.76171903472315 1101 58-59 0.8006916967866857 1101 60-61 0.884151933227109 1101 62-63 0.5796556509792055 1101 64-65 0.7380610752952279 1101 66-67 0.7575218851824772 1101 68-69 0.7153180805712225 1101 70-71 0.8953690907298721 1101 72-73 0.6620700724116304 1101 74-75 0.8269736937625396 1101 76-77 0.6680312117343661 1101 78-79 0.735131163174664 1101 80-81 0.6375605357260028 1101 82-83 0.6382762083512787 1101 84-85 0.6789425687785204 1101 86-87 0.7148690441522625 1101 88-89 0.7844075729339828 1101 90-91 0.8474054225875882 1101 92-93 0.7785206468466548 1101 94-95 0.7290820513007894 1101 96-97 0.9647152789696847 1101 98-99 0.8927217290408826 1101 100-101 0.7664924339040304 2304 1 0.07649143945089065 2304 2 0.07730762873378438 2304 3 0.043154909903108774 2304 4 0.19799413321597825 2304 5 0.15946973403205078 2304 6 0.12862244802683875 2304 7 0.260134393391084 2304 8 0.15597826262532521 2304 9 0.1362500349189304 2304 10-11 0.16088475182795747 2304 12-13 0.1105533190736594 2304 14-15 0.0698727218432893 2304 16-17 0.2738730789858934 2304 18-19 0.36703461573628715 2304 20-21 0.34744678265861495 2304 22-23 0.37922652516842703 2304 24-25 0.252980062559395 2304 26-27 0.3385294743103415 2304 28-29 0.23945248965207355 2304 30-31 0.44127002144710303 2304 32-33 0.47586813047235665 2304 34-35 0.6310997125092612 2304 36-37 0.4535998856571126 2304 38-39 0.27410499075076444 2304 40-41 0.4810196342423154 2304 42-43 0.5032316011212359 2304 44-45 0.5718690449415575 2304 46-47 0.5897352696002258 2304 48-49 0.5485740480996455 2304 50-51 0.4791700689553622 2304 52-53 0.49277791620047395 2304 54-55 0.7023224560965033 2304 56-57 0.7785591127135234 2304 58-59 0.7922989365355448 2304 60-61 0.802582376901718 2304 62-63 0.7760377143851471 2304 64-65 0.7743713195815971 2304 66-67 0.7395489194612779 2304 68-69 0.718886527859155 2304 70-71 0.7619955597573167 2304 72-73 0.9130181205653187 2304 74-75 0.8865026211563531 2304 76-77 0.9758998623458339 2304 78-79 0.8828518228730715 2304 80-81 0.8830856291892388 2304 82-83 0.8945024698813882 2304 84-85 0.9286195467110652 2304 86-87 0.8746023385301491 2304 88-89 0.7683834722381455 2304 90-91 0.8534384969467617 2304 92-93 1.0294221869777083 2304 94-95 1.0976509755940747 2304 96-97 1.1740952912525735 2304 98-99 1.1623326119181776 2304 100-101 0.9681971318105731 2202 1 0.10037764750835265 2202 2 0.14265944110051976 2202 3 0.1818748440668756 2202 4 0.09548973789932802 2202 5 0.07556968540301057 2202 6 0.15610907504213856 2202 7 0.12431536821620881 2202 8 0.0960859946419319 2202 9 0.178194448190915 2202 10-11 0.19466883891134046 2202 12-13 0.12972812175199522 2202 14-15 0.20158913975374304 2202 16-17 0.2226395848026712 2202 18-19 0.3116657832072036 2202 20-21 0.4318190940340685 2202 22-23 0.3422048665442574 2202 24-25 0.21340537958329975 2202 26-27 0.33983965274148886 2202 28-29 0.3235255454258308 2202 30-31 0.39755064838758614 2202 32-33 0.3808507362405038 2202 34-35 0.4832861487491158 2202 36-37 0.4080513127322618 2202 38-39 0.3578544764052012 2202 40-41 0.3309485610369123 2202 42-43 0.3011414877781746 2202 44-45 0.4090412665403278 2202 46-47 0.3523077455662573 2202 48-49 0.37541072935501774 2202 50-51 0.4513449090406496 2202 52-53 0.4830839050532063 2202 54-55 0.42816878834504024 2202 56-57 0.6184087367736737 2202 58-59 0.6528392799313494 2202 60-61 0.6890438664464753 2202 62-63 0.5253615837600734 2202 64-65 0.5733949981810795 2202 66-67 0.4876523870855536 2202 68-69 0.5759648580128953 2202 70-71 0.5255826992478347 2202 72-73 0.5575959831321882 2202 74-75 0.555917763489802 2202 76-77 0.6478287517334778 2202 78-79 0.5713295470342175 2202 80-81 0.458067860887887 2202 82-83 0.5977260138158584 2202 84-85 0.5917390993239344 2202 86-87 0.6401598891230513 2202 88-89 0.8128491139543819 2202 90-91 0.7253449421642806 2202 92-93 0.7409323055577453 2202 94-95 0.914953560414336 2202 96-97 0.9908105869545132 2202 98-99 0.8398472941461357 2202 100-101 0.6942940906255224 1102 1 -0.2751449442411946 1102 2 -0.23682455433391425 1102 3 -0.2710302459240239 1102 4 -0.17593134065293015 1102 5 -0.3550430063655199 1102 6 -0.2504642143814877 1102 7 -0.2212619069811339 1102 8 -0.20312856278005142 1102 9 -0.15118220997170084 1102 10-11 -0.16235707843859615 1102 12-13 -0.18899519396944697 1102 14-15 -0.38958407008534834 1102 16-17 -0.16996585560750077 1102 18-19 -0.13067889783269493 1102 20-21 -0.16046058812248276 1102 22-23 -0.10174932797517755 1102 24-25 -0.19193904175204324 1102 26-27 -0.10588882578996817 1102 28-29 -0.15799705796312224 1102 30-31 -0.047322878407442204 1102 32-33 0.06953281931831867 1102 34-35 0.15400369713818662 1102 36-37 0.05292534875190569 1102 38-39 0.08867307677954983 1102 40-41 0.0018974428888043349 1102 42-43 0.06137471992130372 1102 44-45 0.24632418227311348 1102 46-47 0.13610079959717325 1102 48-49 0.006249262235293429 1102 50-51 0.1657275578669939 1102 52-53 0.3236264038474346 1102 54-55 0.3162159410884158 1102 56-57 0.38463917982090123 1102 58-59 0.6122949988510058 1102 60-61 0.5296572929533596 1102 62-63 0.45224056798793555 1102 64-65 0.47690439228382075 1102 66-67 0.3677379950160784 1102 68-69 0.443221097651076 1102 70-71 0.3710392154677322 1102 72-73 0.40786321692957017 1102 74-75 0.35480932702030543 1102 76-77 0.30799462073617434 1102 78-79 0.3519328545207756 1102 80-81 0.45915669221015065 1102 82-83 0.41624509409801647 1102 84-85 0.44563092110405833 1102 86-87 0.3062800486703985 1102 88-89 0.383800469182912 1102 90-91 0.3331861644779224 1102 92-93 0.6705254569233041 1102 94-95 0.6471156044127966 1102 96-97 0.7659773761988156 1102 98-99 0.6801451832646599 1102 100-101 0.48583590633131735 2305 1 0.008124263931499343 2305 2 -0.06227332972684252 2305 3 -0.04481669481887707 2305 4 0.026405130386535802 2305 5 -0.03842533879204524 2305 6 0.04228203587292967 2305 7 0.004346383628409001 2305 8 -0.009793572004340945 2305 9 0.0490984662430094 2305 10-11 0.051187111621466386 2305 12-13 -0.024114742718232662 2305 14-15 -0.07161763427361478 2305 16-17 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17065.0 18 16968.0 19 18018.0 20 19613.0 21 21680.0 22 23569.0 23 25234.0 24 27992.0 25 31388.0 26 35068.0 27 40605.0 28 46601.0 29 55638.0 30 66562.0 31 82002.0 32 105595.0 33 141643.0 34 204345.0 35 329573.0 36 647040.0 37 955533.0 38 318383.0 39 206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.82316540805621 32.60754186438459 19.32652269384874 15.242770033710457 2 30.226012197272418 26.32056207993046 30.434744471589152 13.018681251207976 3 33.845491340247584 24.255350805251734 24.849703277375777 17.0494545771249 4 29.28575510782935 24.26599569220851 29.271936065915316 17.176313134046822 5 29.07696173943916 22.643337614512987 30.497129936272216 17.782570709775637 6 26.689804578015437 23.327769667880023 31.97296772448906 18.009458029615477 7 30.705556946697488 23.25360275518684 26.0681248253512 19.972715472764467 8 27.113436477012197 28.521436593125944 25.125381678581427 19.239745251280425 9 25.041740669048046 26.49741815593625 29.025351598239784 19.43548957677592 10-11 20.11848004173148 31.62264691686913 32.99807168751452 15.260801353884867 12-13 20.297397668343255 30.859767052050564 33.03000466597458 15.8128306136316 14-15 20.367510862838774 30.841330056880317 33.181087026234856 15.610072054046054 16-17 20.04297033580214 30.914087299937325 33.1223271113033 15.920615252957235 18-19 19.408244090808694 31.32911129276169 32.70132235205686 16.56132226437275 20-21 19.374860263495997 31.5847355357696 32.23757238898894 16.80283181174547 22-23 19.43074124805509 31.03747284379424 32.29226484871712 17.239521059433553 24-25 19.362276588095746 30.752510169025605 32.45747370882145 17.427739534057196 26-27 19.35498425387597 31.197265624999996 32.295391230620154 17.152358890503876 28-29 19.12489874285742 31.466088200258618 32.09738090621182 17.311632150672143 30-31 18.61806311537246 32.492785966934726 31.86227705519719 17.026873862495627 32-33 18.32151344989103 32.90610723172721 31.99057296840185 16.78180634997991 34-35 18.74887456442141 32.38086209008967 32.37895386626592 16.491309479223002 36-37 18.755671216867636 31.946881892833524 32.826089952788514 16.47135693751033 38-39 19.040819074213154 31.82449647257059 32.18100287340525 16.953681579811015 40-41 18.948075482785857 31.758066751330734 31.654167736326716 17.639690029556697 42-43 19.19938365868099 31.35604637168297 31.617042795200017 17.82752717443602 44-45 18.873925523132968 31.314243303216426 31.830582925000567 17.981248248650044 46-47 18.96894131598965 31.542956662854515 31.760065822055328 17.728036199100504 48-49 18.93865116180423 32.13045414743961 31.169227413065414 17.761667277690755 50-51 19.486167606886674 32.00260119202109 30.34093221400865 18.170298987083587 52-53 19.59592684318225 31.815806400047276 30.47143130960886 18.116835447161616 54-55 19.963733037349034 31.901552705845006 30.320946867021544 17.81376738978442 56-57 20.088286911253324 31.97308673189213 30.16478052113213 17.77384583572242 58-59 19.938443341849712 32.49981291841516 30.07412975251606 17.48761398721907 60-61 19.715960985442223 32.37497849886983 29.94435144112082 17.964709074567132 62-63 19.532523656932963 32.26391085477059 30.26791791594071 17.935647572355727 64-65 19.408614042872937 31.872249320548395 30.70550475786451 18.013631878714158 66-67 19.84504378130968 31.51687989499996 30.415972410234232 18.222103913456127 68-69 20.25450515888631 31.516361467698502 30.489429154509505 17.739704218905686 70-71 19.975467881890445 31.814787957163848 30.543622232020134 17.666121928925573 72-73 20.074320355723117 31.7989361056515 30.191940778596077 17.934802760029307 74-75 20.47525990918182 31.343411898026087 29.84776201949892 18.33356617329317 76-77 20.54433118402424 31.189867140637833 29.4111280595564 18.85467361578152 78-79 20.377028581809373 31.344465302167855 29.673534576722783 18.60497153929999 80-81 20.234533023267083 31.69087545268462 29.290799147317802 18.783792376730496 82-83 19.87419250303616 31.82119526207876 29.16978863492506 19.134823599960022 84-85 20.035902498254636 31.776816183662287 29.6856191120895 18.501662205993576 86-87 20.406456208042663 31.504945887737694 29.937109623872594 18.151488280347053 88-89 20.865109193237366 31.39681401517062 29.764773436585283 17.97330335500673 90-91 20.88906453947747 31.331022619555966 29.316749495004558 18.463163345962005 92-93 20.66851552970774 31.484848764688707 29.18547737711341 18.66115832849015 94-95 20.58070951054095 31.26044900251094 29.261217873526775 18.89762361342134 96-97 20.75591773661864 31.701141887620526 28.95529514204263 18.5876452337182 98-99 21.34540718979981 31.421666313334747 28.673142736000486 18.559783760864956 100-101 21.808217883472906 31.336015661419637 28.077162721677514 18.77860373342994 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 143792.0 1 81520.0 2 13945.5 3 8471.5 4 7456.5 5 6506.5 6 6693.5 7 7470.5 8 7883.5 9 7634.5 10 7191.0 11 6726.5 12 6330.5 13 6013.5 14 5864.5 15 5843.5 16 5843.5 17 5851.5 18 5938.0 19 6070.5 20 6214.0 21 6418.0 22 6849.0 23 7560.0 24 8490.5 25 10115.5 26 12479.0 27 15392.0 28 18743.5 29 22376.5 30 26945.5 31 32616.0 32 41340.5 33 55697.5 34 75335.5 35 96474.5 36 124461.5 37 161296.5 38 188738.0 39 193021.0 40 191987.5 41 191077.5 42 183805.5 43 178541.5 44 167735.5 45 155047.0 46 151150.5 47 140124.0 48 116484.0 49 93382.0 50 74317.0 51 59865.0 52 48455.5 53 37809.5 54 30176.5 55 25322.0 56 20029.5 57 14814.5 58 10820.5 59 7651.0 60 5519.5 61 4291.5 62 3257.5 63 2534.0 64 2082.5 65 1536.5 66 1043.0 67 769.5 68 595.0 69 480.0 70 375.5 71 279.0 72 243.5 73 191.5 74 142.0 75 130.0 76 107.0 77 91.5 78 75.5 79 54.0 80 43.5 81 31.0 82 24.5 83 22.5 84 20.0 85 16.5 86 12.0 87 6.0 88 4.5 89 6.5 90 3.0 91 3.0 92 4.0 93 3.5 94 3.5 95 1.0 96 1.5 97 1.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23021320518634447 2 0.24650614500294027 3 0.22710547777688267 4 0.21989434330250043 5 0.22924769764165728 6 0.2517862643879482 7 0.2318123270572326 8 0.2177521234377258 9 0.21099357062491572 10-11 0.19625449451305077 12-13 0.2232736197089055 14-15 0.2198792572471147 16-17 0.22286629621349063 18-19 0.21097848456952997 20-21 0.20379752220591923 22-23 0.17146810551428515 24-25 0.5033462379451104 26-27 0.35962138828519524 28-29 0.4949282190398691 30-31 0.3329492423632125 32-33 0.3846793262809042 34-35 0.3867913740349073 36-37 0.39554128615863465 38-39 0.3872741278072509 40-41 0.3933387220723171 42-43 0.4480105867902275 44-45 0.40110804059597155 46-47 0.41955828633272774 48-49 0.41785356207413954 50-51 0.4312650653120596 52-53 0.43512709549080825 54-55 0.3995692629466264 56-57 0.41385575739691927 58-59 0.41094414870747203 60-61 0.3671040717565207 62-63 0.3288910934644492 64-65 0.3572227054788631 66-67 0.3688540541812662 68-69 0.35936492534363773 70-71 0.3287100607998204 72-73 0.3172144865958889 74-75 0.32904195401830666 76-77 0.3368565307081183 78-79 0.34755254397660573 80-81 0.3560007349926184 82-83 0.3756126069940763 84-85 0.40534722215936364 86-87 0.4072028069718093 88-89 0.4003084796605275 90-91 0.37138851148607 92-93 0.3490460634597937 94-95 0.32507432145185783 96-97 0.34296638313934175 98-99 0.3747074436709321 100-101 0.3909702113767564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3314319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.57171062190845 #Duplication Level Percentage of deduplicated Percentage of total 1 82.67730494345228 36.85068910938836 2 8.190777687205422 7.301539456850094 3 2.807053472315067 3.7534552520475133 4 1.5825569777807331 2.821490866252993 5 0.9544444991748524 2.1270612010946928 6 0.6289582026903789 1.6820245802154725 7 0.5082627392842899 1.5857897814694508 8 0.3808164999110047 1.357891426726506 9 0.29520982425304715 1.184220617341614 >10 1.6825982844534695 14.434042552876427 >50 0.16256210513295596 5.055226217630826 >100 0.11907536235203658 10.949920149734552 >500 0.008857349083181816 2.660273444808742 >1k 0.0013949308218554927 0.8037372748810501 >5k 0.0 0.0 >10k+ 1.271220894738575E-4 7.43263806868172 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 145603 4.393149844658888 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116754 3.5227146210126423 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.5086055385736858E-4 0.0 0.0 2 2.4137688617178975E-4 0.0 0.0 3 2.7154899694326344E-4 0.0 0.0 4 2.7154899694326344E-4 0.0 0.0 5 3.620653292576846E-4 0.0 0.0 6 4.2240955080063205E-4 0.0 0.0 7 4.827537723435795E-4 0.0 0.0 8 8.448191016012641E-4 0.0 0.0 9 0.0010861959877730538 0.0 0.0 10-11 0.002670231803275424 0.0 0.0 12-13 0.00974559177918601 0.0 0.0 14-15 0.021618317367760917 0.0 0.0 16-17 0.045891780483411526 0.0 0.0 18-19 0.06776656079272997 0.0 0.0 20-21 0.07556605142715593 0.0 0.0 22-23 0.08347114444928204 0.0 0.0 24-25 0.09214562629608072 0.0 0.0 26-27 0.10208733679528133 0.0 0.0 28-29 0.1136583412761415 0.0 0.0 30-31 0.1265418325755608 0.0 0.0 32-33 0.14078306885969638 0.0 0.0 34-35 0.15505447725460342 0.0 0.0 36-37 0.17084957724346994 0.0 0.0 38-39 0.18824379910322453 0.0 0.0 40-41 0.20771989660621082 0.0 0.0 42-43 0.2289158044231711 0.0 0.0 44-45 0.2515901456679336 0.0 0.0 46-47 0.27536576895585485 0.0 0.0 48-49 0.30064999778234985 0.0 0.0 50-51 0.3284837699690344 0.0 0.0 52-53 0.3589726879036086 0.0 0.0 54-55 0.3900650480536122 0.0 0.0 56-57 0.4211272360928444 0.0 0.0 58-59 0.45549327026155295 1.5086055385736859E-5 0.0 60-61 0.49299720395049484 3.0172110771473718E-5 0.0 62-63 0.5330355949442405 3.0172110771473718E-5 0.0 64-65 0.5768756718951917 3.0172110771473718E-5 0.0 66-67 0.6243816603048771 4.5258166157210576E-5 0.0 68-69 0.6745880526286094 6.0344221542947436E-5 0.0 70-71 0.727117697481745 6.0344221542947436E-5 0.0 72-73 0.7865416696461627 6.0344221542947436E-5 0.0 74-75 0.8515323962479171 6.0344221542947436E-5 0.0 76-77 0.9263441449057861 6.0344221542947436E-5 0.0 78-79 1.009649342745825 6.0344221542947436E-5 0.0 80-81 1.1062151832699265 6.0344221542947436E-5 0.0 82-83 1.2174748417397359 6.0344221542947436E-5 0.0 84-85 1.3451330424138412 6.0344221542947436E-5 0.0 86-87 1.4918298449847467 6.0344221542947436E-5 3.0172110771473718E-5 88-89 1.6602203952003411 6.0344221542947436E-5 3.0172110771473718E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGCC 70 8.0179234E-8 47.43128 1 CGGTGCG 60 1.5081114E-6 47.43128 1 CGTACCC 40 5.428263E-4 47.43128 1 CGCAGCC 160 0.0 44.466824 1 CGCGGAG 150 0.0 44.26919 1 CCGCACG 50 0.0016277913 37.945023 1 CGGGTCG 90 5.767306E-7 36.89099 1 ACGAGTC 65 1.3400897E-4 36.4856 1 ATTGACG 80 1.08897E-5 35.57346 1 TGCCGGC 80 1.08953445E-5 35.57077 2 CGCGAGG 70 2.0726274E-4 33.876923 2 TTGGCCG 70 2.0729957E-4 33.875896 3 GCTGCGG 100 1.3157405E-6 33.199383 2 CGGAGCG 60 0.003980124 31.620852 1 CGACTGG 75 3.1078816E-4 31.617502 3 CCGTTGT 155 1.382432E-9 30.595734 7 CGGGGTG 685 0.0 30.466805 1 CGACGGG 125 2.1835149E-7 30.356016 1 GTTTGAT 14385 0.0 30.13389 3 CGGGCTG 425 0.0 30.13281 1 >>END_MODULE