FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3314319
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1456034.393149844658888No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1167543.5227146210126423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC708.0179234E-847.431281
CGGTGCG601.5081114E-647.431281
CGTACCC405.428263E-447.431281
CGCAGCC1600.044.4668241
CGCGGAG1500.044.269191
CCGCACG500.001627791337.9450231
CGGGTCG905.767306E-736.890991
ACGAGTC651.3400897E-436.48561
ATTGACG801.08897E-535.573461
TGCCGGC801.08953445E-535.570772
CGCGAGG702.0726274E-433.8769232
TTGGCCG702.0729957E-433.8758963
GCTGCGG1001.3157405E-633.1993832
CGGAGCG600.00398012431.6208521
CGACTGG753.1078816E-431.6175023
CCGTTGT1551.382432E-930.5957347
CGGGGTG6850.030.4668051
CGACGGG1252.1835149E-730.3560161
GTTTGAT143850.030.133893
CGGGCTG4250.030.132811
Produced by FastQC (version 0.11.2)