##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_50110001-2.342000000358f4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5873284 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.219064155589955 33.0 33.0 33.0 33.0 33.0 2 32.20339166299467 33.0 33.0 33.0 33.0 33.0 3 32.19826539973207 33.0 33.0 33.0 33.0 33.0 4 35.95499979227975 37.0 37.0 37.0 33.0 37.0 5 35.936120575814144 37.0 37.0 37.0 33.0 37.0 6 35.881286687311565 37.0 37.0 37.0 33.0 37.0 7 35.844931217356425 37.0 37.0 37.0 33.0 37.0 8 35.75122912496654 37.0 37.0 37.0 33.0 37.0 9 35.69167556004443 37.0 37.0 37.0 33.0 37.0 10-11 35.60318247849074 37.0 37.0 37.0 33.0 37.0 12-13 35.6801813091279 37.0 37.0 37.0 33.0 37.0 14-15 37.46214519849542 40.0 37.0 40.0 33.0 40.0 16-17 37.4028828675746 40.0 37.0 40.0 33.0 40.0 18-19 37.36349536307115 40.0 37.0 40.0 33.0 40.0 20-21 37.31136107159129 40.0 37.0 40.0 33.0 40.0 22-23 37.29860040822136 40.0 37.0 40.0 33.0 40.0 24-25 37.12593150612162 40.0 37.0 40.0 33.0 40.0 26-27 37.05342871211404 40.0 37.0 40.0 33.0 40.0 28-29 36.95610666877338 40.0 37.0 40.0 33.0 40.0 30-31 37.004224297684225 40.0 37.0 40.0 33.0 40.0 32-33 36.93080114974859 40.0 37.0 40.0 33.0 40.0 34-35 36.810523890893066 40.0 37.0 40.0 33.0 40.0 36-37 36.74266977384373 40.0 37.0 40.0 33.0 40.0 38-39 36.706040096137016 40.0 37.0 40.0 33.0 40.0 40-41 36.57443186809969 40.0 37.0 40.0 33.0 40.0 42-43 36.46114465093123 40.0 37.0 40.0 33.0 40.0 44-45 36.364208337277745 40.0 37.0 40.0 33.0 40.0 46-47 36.24405988200128 37.0 37.0 40.0 33.0 40.0 48-49 36.09174066842333 37.0 37.0 40.0 30.0 40.0 50-51 34.86218093659357 37.0 35.0 38.5 27.0 38.5 52-53 35.30802213889197 37.0 37.0 38.5 30.0 40.0 54-55 36.08520343984728 37.0 37.0 40.0 33.0 40.0 56-57 36.101205475505694 37.0 37.0 40.0 33.0 40.0 58-59 35.92686067964702 37.0 37.0 40.0 30.0 40.0 60-61 35.6621814984598 37.0 37.0 40.0 27.0 40.0 62-63 35.362716497278186 37.0 37.0 40.0 27.0 40.0 64-65 35.07876087721963 37.0 35.0 38.5 27.0 40.0 66-67 34.82074559990629 37.0 33.0 37.0 27.0 40.0 68-69 34.623663524529036 37.0 33.0 37.0 27.0 40.0 70-71 34.358339048477816 37.0 33.0 37.0 27.0 40.0 72-73 34.111062397118886 37.0 33.0 37.0 27.0 38.5 74-75 33.855970612011944 37.0 33.0 37.0 27.0 37.0 76-77 33.6517185274882 37.0 33.0 37.0 27.0 37.0 78-79 33.43829133752088 37.0 33.0 37.0 27.0 37.0 80-81 33.234910571326026 37.0 33.0 37.0 27.0 37.0 82-83 33.05517960650294 37.0 33.0 37.0 27.0 37.0 84-85 32.926614480076225 37.0 33.0 37.0 27.0 37.0 86-87 32.73903330743073 37.0 33.0 37.0 27.0 37.0 88-89 32.62109205003539 37.0 33.0 37.0 24.5 37.0 90-91 32.513343301635 35.0 33.0 37.0 22.0 37.0 92-93 32.36172250482013 33.0 33.0 37.0 22.0 37.0 94-95 32.24432532123426 33.0 33.0 37.0 22.0 37.0 96-97 32.08010194977801 33.0 33.0 37.0 22.0 37.0 98-99 31.942988113634552 33.0 33.0 37.0 22.0 37.0 100-101 30.58644107793868 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.029592943170236197 2306 2 0.051860638806012105 2306 3 3.408434966445384E-5 2306 4 0.10212025476671727 2306 5 0.11739511541967573 2306 6 0.0773692658438776 2306 7 0.14236094126862042 2306 8 0.06986772139725872 2306 9 0.1303783000154226 2306 10-11 0.16915132761021567 2306 12-13 0.06469582545754093 2306 14-15 0.17722238384983058 2306 16-17 0.23120909725828653 2306 18-19 0.24162642999279882 2306 20-21 0.18119006746988475 2306 22-23 0.3103921094641535 2306 24-25 0.1262210025454138 2306 26-27 0.18087942505460575 2306 28-29 0.1282684109567782 2306 30-31 0.30476794590676803 2306 32-33 0.3053866075272822 2306 34-35 0.32307635359066467 2306 36-37 0.36646559589399175 2306 38-39 0.22852398827621556 2306 40-41 0.24854219674019618 2306 42-43 0.3178553673829043 2306 44-45 0.37612009360650944 2306 46-47 0.2652310771356312 2306 48-49 0.3293141033345677 2306 50-51 0.24715751901813832 2306 52-53 0.2489849236970869 2306 54-55 0.4267799320649672 2306 56-57 0.34124200612749434 2306 58-59 0.36964711785124393 2306 60-61 0.3720591377619513 2306 62-63 0.3680026479464473 2306 64-65 0.2627717042211799 2306 66-67 0.21417931801567391 2306 68-69 0.18258017436967577 2306 70-71 0.19848854471259614 2306 72-73 0.2062272265649412 2306 74-75 0.2371570089412316 2306 76-77 0.1582232933950749 2306 78-79 0.20934735655585968 2306 80-81 0.12581904706716074 2306 82-83 0.14316290528785203 2306 84-85 0.19774890314521087 2306 86-87 0.23444682493962432 2306 88-89 0.21527663015931608 2306 90-91 0.2325089929231723 2306 92-93 0.18515283133557858 2306 94-95 0.17273624745682525 2306 96-97 0.251512827593551 2306 98-99 0.1755480969124399 2306 100-101 -0.0924728139511295 2307 1 0.14605546518583168 2307 2 0.13506626260039667 2307 3 0.10805919928824892 2307 4 0.1471635997654559 2307 5 0.20006321548295602 2307 6 0.20136379148871697 2307 7 0.20367295663950813 2307 8 0.17751256688777772 2307 9 0.3072941337109185 2307 10-11 0.2262274844299057 2307 12-13 0.11974387855421043 2307 14-15 0.23552655366157893 2307 16-17 0.25754871313849037 2307 18-19 0.22908563918483793 2307 20-21 0.22213193485019644 2307 22-23 0.24705341995463215 2307 24-25 0.2737307618415272 2307 26-27 0.28003492190189405 2307 28-29 0.12295941105590202 2307 30-31 0.2640996056733016 2307 32-33 0.30666465597303727 2307 34-35 0.41993927360255867 2307 36-37 0.36233123783457444 2307 38-39 0.2838932213327823 2307 40-41 0.24386983842154564 2307 42-43 0.3391709471842148 2307 44-45 0.17472262721127407 2307 46-47 0.2812733966458367 2307 48-49 0.2458816521501035 2307 50-51 0.03554948437263761 2307 52-53 0.10170961624113772 2307 54-55 0.0722539631879826 2307 56-57 0.21637209830709025 2307 58-59 0.24808383872775153 2307 60-61 0.32270988457683103 2307 62-63 0.3638630480775902 2307 64-65 0.29592710195061755 2307 66-67 0.2673026739936333 2307 68-69 0.0888208820711327 2307 70-71 0.2441204019523937 2307 72-73 0.11157301443748224 2307 74-75 0.20650100125579485 2307 76-77 0.13627869646162338 2307 78-79 0.06291121513756082 2307 80-81 0.13786377209136447 2307 82-83 0.018068552718034425 2307 84-85 0.07770670181398032 2307 86-87 0.005854851198229483 2307 88-89 0.06623102069890052 2307 90-91 0.10032056607595052 2307 92-93 0.09957552703615136 2307 94-95 0.021501944293440545 2307 96-97 0.18511149178988973 2307 98-99 0.07362349509934418 2307 100-101 -0.12061180005175487 2201 1 0.1372825658730079 2201 2 0.13029957964632644 2201 3 0.14452601564390477 2201 4 0.21826334847409612 2201 5 0.20046787988489712 2201 6 0.2044282469465486 2201 7 0.22626086983540716 2201 8 0.1661308716018155 2201 9 0.25356771166552505 2201 10-11 0.13096358002920994 2201 12-13 0.09316642419772592 2201 14-15 0.17209826864754518 2201 16-17 0.2568507785362897 2201 18-19 0.2509279674303002 2201 20-21 0.2938487628397155 2201 22-23 0.16426086717569177 2201 24-25 0.10280552102064888 2201 26-27 0.15616718724695744 2201 28-29 0.1279106361012694 2201 30-31 0.2646381079302742 2201 32-33 0.3584064870649115 2201 34-35 0.3955051639030174 2201 36-37 0.33281933639661787 2201 38-39 0.3083211747818311 2201 40-41 0.25682966670577656 2201 42-43 0.3223501803577804 2201 44-45 0.33072002704374626 2201 46-47 0.2537837023060945 2201 48-49 0.2561451881452683 2201 50-51 0.1816410893798519 2201 52-53 0.28235295895911605 2201 54-55 0.3586316350976375 2201 56-57 0.3849122525720645 2201 58-59 0.5294407732834117 2201 60-61 0.4107483383593902 2201 62-63 0.43530429902768475 2201 64-65 0.46403355564009985 2201 66-67 0.3761841559921706 2201 68-69 0.34091492824979497 2201 70-71 0.3027201506610382 2201 72-73 0.37330382244778804 2201 74-75 0.2854129833551653 2201 76-77 0.29966165789925725 2201 78-79 0.2764030501073975 2201 80-81 0.2577645061878826 2201 82-83 0.2356443570054978 2201 84-85 0.23583843006254313 2201 86-87 0.23345913905092885 2201 88-89 0.27803192271078103 2201 90-91 0.29375810000804137 2201 92-93 0.14482513148494291 2201 94-95 0.16530962884522893 2201 96-97 0.28470207689544935 2201 98-99 0.31946957720336755 2201 100-101 0.17925140515400528 1101 1 0.05710565570873882 1101 2 0.005013090271866361 1101 3 -0.025643779674226153 1101 4 0.03837314809464232 1101 5 0.026833806913202807 1101 6 0.1088491936883571 1101 7 0.04203290558067607 1101 8 0.04651756833833076 1101 9 0.10256277271037817 1101 10-11 0.06834374292127166 1101 12-13 0.045287873827781766 1101 14-15 0.1331347879978111 1101 16-17 0.12757292219446725 1101 18-19 0.11866401369179158 1101 20-21 0.07062610782952561 1101 22-23 0.02954720667110422 1101 24-25 -0.10932013403891716 1101 26-27 0.03434387347430601 1101 28-29 -0.008414995506633716 1101 30-31 0.09048600451020405 1101 32-33 0.13603900217624698 1101 34-35 0.2068917830550916 1101 36-37 0.1347797983061909 1101 38-39 0.07246038860456139 1101 40-41 0.14699782217089563 1101 42-43 0.05171159426373606 1101 44-45 0.04374405265367187 1101 46-47 0.08926480961243755 1101 48-49 0.031015123997036653 1101 50-51 0.01904516679501711 1101 52-53 0.12348794442966238 1101 54-55 0.15464862168257554 1101 56-57 0.21223822096698086 1101 58-59 0.30160373940722707 1101 60-61 0.30300311861908824 1101 62-63 0.15640323418120516 1101 64-65 0.2195450046353784 1101 66-67 0.18149102009949303 1101 68-69 0.2866327739061276 1101 70-71 0.20518063549327792 1101 72-73 0.11419294017159842 1101 74-75 0.10210364536205674 1101 76-77 0.09978391952106591 1101 78-79 0.0801877823705297 1101 80-81 0.0698885963339535 1101 82-83 -0.042704403583016415 1101 84-85 -0.016051081754973495 1101 86-87 0.04665195490778018 1101 88-89 -0.034175857245635655 1101 90-91 0.11689545621233322 1101 92-93 0.04088176989206005 1101 94-95 0.1446118625718853 1101 96-97 0.19259140092335514 1101 98-99 0.08981916124495726 1101 100-101 -0.05186361554693519 2304 1 0.02672399798380809 2304 2 0.034974704539706636 2304 3 -0.011313596205056342 2304 4 0.11369086292653208 2304 5 0.08640485103418882 2304 6 0.059150756863559195 2304 7 0.15470846876856825 2304 8 0.1333413660306917 2304 9 0.12930192568337162 2304 10-11 0.1355154615531049 2304 12-13 0.10060172671725098 2304 14-15 0.09041537261028765 2304 16-17 0.18587909785719603 2304 18-19 0.1296132107185528 2304 20-21 0.1471624144650221 2304 22-23 0.13502752301149457 2304 24-25 0.1133972584263816 2304 26-27 0.05895757282566194 2304 28-29 0.054493930511192445 2304 30-31 0.04290661685896424 2304 32-33 0.11579749304897291 2304 34-35 0.1247622851951391 2304 36-37 0.09551539193187608 2304 38-39 0.007445101849519631 2304 40-41 0.05245865212473433 2304 42-43 0.0032275733014728303 2304 44-45 0.06488196366967003 2304 46-47 0.11713769043276301 2304 48-49 0.029233583214519854 2304 50-51 -0.0917980817883901 2304 52-53 0.08289928289667614 2304 54-55 0.14633492531059744 2304 56-57 0.18786074335906733 2304 58-59 0.17843096458352647 2304 60-61 0.21450089376602222 2304 62-63 0.20392335559186847 2304 64-65 0.1963962575341469 2304 66-67 0.18312250807643693 2304 68-69 0.19267887806228856 2304 70-71 0.17256364469729846 2304 72-73 0.27626267870869725 2304 74-75 0.23294633902714423 2304 76-77 0.15022659738182398 2304 78-79 0.1436178176500036 2304 80-81 0.01564290098636434 2304 82-83 0.08877688783960735 2304 84-85 0.20921054296074715 2304 86-87 0.12980435756357878 2304 88-89 0.14247496252773573 2304 90-91 0.005693879762453946 2304 92-93 0.13609503319486294 2304 94-95 0.16830639312504303 2304 96-97 0.28639600687392175 2304 98-99 0.16291525535075024 2304 100-101 0.1039172561213384 2202 1 0.015850649527649807 2202 2 0.004476977250490677 2202 3 0.04821813134170583 2202 4 0.047435218344652696 2202 5 0.11937731517099337 2202 6 0.09744885968012795 2202 7 0.08947995797661434 2202 8 0.059847962678269084 2202 9 0.032526236766429406 2202 10-11 0.0740411799146301 2202 12-13 0.045376914178596905 2202 14-15 0.11922251954710816 2202 16-17 0.11561360416566657 2202 18-19 0.022050326670218112 2202 20-21 0.08463325847224468 2202 22-23 0.1625268324290161 2202 24-25 -0.037661914432810306 2202 26-27 0.08822993305810911 2202 28-29 0.09722328616274467 2202 30-31 0.15667851349051176 2202 32-33 0.16356524244781667 2202 34-35 0.2765047664211622 2202 36-37 0.2415696099671365 2202 38-39 0.20273814371709875 2202 40-41 0.2044866559496299 2202 42-43 0.2034519493934539 2202 44-45 0.3136089759214684 2202 46-47 0.1623009782840299 2202 48-49 0.20208586437939147 2202 50-51 0.16165008502203904 2202 52-53 0.2789790097917333 2202 54-55 0.2201983372211842 2202 56-57 0.3654981802657744 2202 58-59 0.3919538081137688 2202 60-61 0.38551918282328757 2202 62-63 0.28690864928162796 2202 64-65 0.32536512793030425 2202 66-67 0.35579357776065734 2202 68-69 0.41454761879970903 2202 70-71 0.25602166398874715 2202 72-73 0.3452354877519639 2202 74-75 0.40070263820572904 2202 76-77 0.29045438076267516 2202 78-79 0.26440692273939703 2202 80-81 0.2521777336440607 2202 82-83 0.27622305117297685 2202 84-85 0.3133182059600159 2202 86-87 0.3600218904944157 2202 88-89 0.3070532081059767 2202 90-91 0.26392016639877625 2202 92-93 0.3141828603347179 2202 94-95 0.33468099598972856 2202 96-97 0.30855859237954775 2202 98-99 0.176405213303088 2202 100-101 0.14471332896622613 1102 1 -0.13872389429641174 1102 2 -0.18257026046906333 1102 3 -0.1784148435525168 1102 4 -0.12952066673205564 1102 5 -0.14154659896217225 1102 6 -0.08977555397999737 1102 7 -0.15027746946711318 1102 8 -0.09298999223957338 1102 9 -0.1512509054792872 1102 10-11 -0.08452665484715283 1102 12-13 -0.11946352788579162 1102 14-15 -0.2858269749075717 1102 16-17 -0.1521282916149147 1102 18-19 -0.15400245740070773 1102 20-21 -0.19078258884530896 1102 22-23 -0.20025021962405276 1102 24-25 -0.26638659109416096 1102 26-27 -0.1581619971948527 1102 28-29 -0.2019906812965715 1102 30-31 -0.05893658281279812 1102 32-33 0.024454270621731666 1102 34-35 0.06555040648024857 1102 36-37 -0.010077762333345675 1102 38-39 0.0882137828689693 1102 40-41 -0.07065561914296126 1102 42-43 0.09014318754525164 1102 44-45 -0.029450958116804316 1102 46-47 -0.026013638145442997 1102 48-49 0.08528008620049832 1102 50-51 0.2046353504024765 1102 52-53 0.16252594573318646 1102 54-55 0.1863455403525549 1102 56-57 0.17744413323312358 1102 58-59 0.20373806942441774 1102 60-61 0.21005801088930554 1102 62-63 0.30259843078581383 1102 64-65 0.2607844479624646 1102 66-67 0.25567984106349684 1102 68-69 0.27381759635477465 1102 70-71 0.27628051687991473 1102 72-73 0.23491745908922468 1102 74-75 0.2229637876676449 1102 76-77 0.19557343869355037 1102 78-79 0.2535900420642676 1102 80-81 0.16296996188346924 1102 82-83 0.27749607105896246 1102 84-85 0.3270905004337763 1102 86-87 0.1405344990357733 1102 88-89 0.13284244172458415 1102 90-91 0.14792924865916746 1102 92-93 0.21014351026966693 1102 94-95 0.1677121762422118 1102 96-97 0.28591198350719793 1102 98-99 0.09103623704519137 1102 100-101 0.11627124710293657 2305 1 -0.003614519630936286 2305 2 -0.034607789140096656 2305 3 -0.0959237375923152 2305 4 -0.12227059175422994 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12864.0 16 14127.0 17 14699.0 18 15854.0 19 16950.0 20 19148.0 21 21517.0 22 23856.0 23 26307.0 24 30193.0 25 34807.0 26 40827.0 27 48592.0 28 59458.0 29 72205.0 30 89722.0 31 116205.0 32 158454.0 33 226041.0 34 344543.0 35 578803.0 36 1127707.0 37 2027723.0 38 645072.0 39 616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.732499841297034 28.895355702828873 16.506899995290105 16.865244460583988 2 33.69778153111253 23.6326280242345 28.373691732806105 14.295898711846867 3 39.69936033314688 21.141615750077044 21.191767296823098 17.96725661995298 4 33.36983069252161 20.58244598169024 27.563986405714815 18.483736920073344 5 34.859146697837524 18.55973584406609 27.237138822646077 19.343978635450306 6 31.568348835641398 19.339165580694356 29.489051094890513 19.603434488773736 7 36.099011158047134 19.451223944903624 22.798057879319167 21.651707017730075 8 30.79939352658218 26.027681013819347 22.567605474231364 20.605319985367103 9 29.382630911039627 23.215628078510967 26.530839961959966 20.870901048489436 10-11 22.805201513572655 30.268450771572176 31.08920817372906 15.837139541126108 12-13 23.193308713526207 29.014586318559598 31.314074029203354 16.478030938710845 14-15 22.882286361112232 28.699857858312694 31.710768753535735 16.707087027039343 16-17 22.229809772326572 29.053400512159634 31.84261009448462 16.87417962102917 18-19 21.410989572705454 29.499240422879037 31.649592674209657 17.44017733020585 20-21 21.737486723984876 29.42830755640371 30.85144178526545 17.982763934345964 22-23 21.534179225489872 28.175625917331605 31.351030123097672 18.939164734080855 24-25 20.747898002085954 28.287197897959302 31.735937554811944 19.228966545142796 26-27 20.601167583778903 28.890171737268705 31.4700667825388 19.03859389641359 28-29 20.625055661576926 28.93282138513294 30.78542687734896 19.65669607594117 30-31 20.099149981962483 30.578478234728234 30.203405469740694 19.118966313568585 32-33 19.492472301655482 31.04484934947705 31.333534876304853 18.129143472562614 34-35 19.67561286792135 30.512927368621906 32.24026509195615 17.571194671500606 36-37 19.702756528772575 30.157244300155828 32.829812025529286 17.31018714554231 38-39 20.16800769875066 29.641343630919348 31.81235098496259 18.3782976853674 40-41 20.214996150253757 29.49424332618075 31.125809365351596 19.16495115821389 42-43 20.57565368573579 28.813960891248136 31.263719318382066 19.346666104634007 44-45 20.103555376770153 28.772408318854026 31.610438572044746 19.513597732331075 46-47 20.589939568137716 29.11099541232764 31.002505265211163 19.296559754323482 48-49 20.47823143980322 29.921484301176775 30.16887751792026 19.431406741099746 50-51 21.12821023992343 29.845471813166576 28.838607759689655 20.187710187220343 52-53 21.320251492921834 29.75011004931252 28.823661119417586 20.105977338348055 54-55 21.79103829634785 29.622760736364096 28.791365101330058 19.794835865957996 56-57 21.7455899848567 30.28462687025148 28.59760659436596 19.37217655052586 58-59 21.20481940068093 31.223590536585082 28.425834867679818 19.14575519505417 60-61 20.910827404036986 31.27476639218842 28.11279301255187 19.701613191222734 62-63 20.772415915815635 31.044486717149088 28.932345092618956 19.250752274416318 64-65 20.67288672804036 30.1112129478466 29.66740163953822 19.548498684574824 66-67 21.143516703019365 29.374229480801418 29.33433121710209 20.147922599077127 68-69 21.28230646872592 30.02450909202741 29.82240518851242 18.870779250734248 70-71 20.613981673621574 30.809423872185747 29.707525047308785 18.869069406883895 72-73 20.362386925938832 30.54089478255427 29.530961625545864 19.565756665961036 74-75 20.60851680295741 29.60187832448086 29.24240758879438 20.54719728376735 76-77 20.56629052953147 29.00367231853146 28.811946834441272 21.6180903174958 78-79 19.900939855034892 29.66852708381683 29.133148564336008 21.29738449681227 80-81 19.484733209161135 30.25435653412634 28.766323228367845 21.494587028344682 82-83 18.715271767297835 30.450524406246227 28.790361731544532 22.043842094911405 84-85 18.4443019261001 30.767305167461807 29.887011289469857 20.901381616968237 86-87 18.99420723728687 30.40352594624041 30.539084695971834 20.063182120500883 88-89 19.782218359360645 30.208150125158422 29.96493513041858 20.04469638506235 90-91 19.750995001582297 30.318986605498193 29.419743032254935 20.51027536066458 92-93 19.201051467908858 30.827162271798 29.540058812364705 20.431727447928438 94-95 19.339200762033006 30.232091622274094 29.788989992851246 20.639717622841655 96-97 19.736398294643365 30.802229109359857 29.35045523011766 20.110917365879118 98-99 20.541155283792023 30.57392701976958 28.747417721002027 20.137499975436363 100-101 20.326454960443943 31.071790524662507 27.922003900061355 20.679750614832194 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15153.0 1 9016.5 2 2262.0 3 1581.0 4 1360.5 5 1208.5 6 1329.5 7 1533.5 8 1796.0 9 2121.5 10 2418.0 11 2629.5 12 2655.0 13 2439.5 14 2127.0 15 1907.5 16 1782.5 17 1792.0 18 1898.0 19 2090.5 20 2544.0 21 3193.5 22 3984.0 23 5202.0 24 6924.5 25 9081.0 26 11935.5 27 15653.5 28 20305.5 29 26031.5 30 33619.5 31 42700.0 32 59287.5 33 92884.5 34 138973.0 35 181335.0 36 235985.5 37 318354.5 38 397163.0 39 440089.5 40 441950.0 41 429820.0 42 430846.0 43 423781.5 44 374833.5 45 311692.0 46 271190.5 47 237434.0 48 200920.5 49 161366.0 50 117315.0 51 87280.5 52 70924.0 53 54609.5 54 39685.0 55 29111.5 56 21925.0 57 16753.5 58 12581.5 59 9273.5 60 6923.0 61 5568.5 62 4506.5 63 3470.0 64 2596.5 65 1962.5 66 1503.0 67 1235.0 68 1038.5 69 895.0 70 791.0 71 624.5 72 444.0 73 330.0 74 272.0 75 230.5 76 196.5 77 139.0 78 98.0 79 75.5 80 57.0 81 46.5 82 38.0 83 30.0 84 22.0 85 24.0 86 24.5 87 13.0 88 9.0 89 13.0 90 9.0 91 8.0 92 7.5 93 5.0 94 4.5 95 2.5 96 2.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22610859614484846 2 0.24173869337835527 3 0.22212445371277806 4 0.21429237884631494 5 0.2231971074444893 6 0.24652306954678166 7 0.22641506863962305 8 0.21310054136663578 9 0.20584735899030251 10-11 0.19170705860639467 12-13 0.21838719190149838 14-15 0.21423278697233097 16-17 0.21804666690730434 18-19 0.20649435647927122 20-21 0.19885808348446965 22-23 0.16702750965218097 24-25 0.5012357652039302 26-27 0.35509946394555414 28-29 0.4905688197608016 30-31 0.3204340195366 32-33 0.3705082199328349 34-35 0.3725428567731443 36-37 0.38064735163496266 38-39 0.3736410498794201 40-41 0.37881702979116966 42-43 0.4335308832332984 44-45 0.3864022240368421 46-47 0.4052843349649021 48-49 0.403343342497996 50-51 0.4168111060183706 52-53 0.4217316921844747 54-55 0.3864958684102454 56-57 0.40015943380228164 58-59 0.3974777994730035 60-61 0.3549462276981668 62-63 0.3184760008199842 64-65 0.34450062350126437 66-67 0.35583159268307135 68-69 0.3465522865912835 70-71 0.3177608983321767 72-73 0.3063958766509503 74-75 0.31846748769512934 76-77 0.32486935758597746 78-79 0.3348807924152825 80-81 0.3426021966586325 82-83 0.3615864650849508 84-85 0.3910078245833166 86-87 0.3925061345577704 88-89 0.38647884216053574 90-91 0.35809608389446174 92-93 0.3363195105157524 94-95 0.3136150065278641 96-97 0.32990912750004936 98-99 0.3598923532388354 100-101 0.37617796108616575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5873284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.66163264570327 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33247255855103 30.63113703666891 2 8.853736781635973 6.668923644234505 3 3.056064129497843 3.452890937605761 4 1.6415929293565519 2.473002794368415 5 0.9094464187676821 1.7125618467289434 6 0.6752757421795527 1.5259192161912567 7 0.4533324737117224 1.1951268763909177 8 0.39329879813934654 1.184981988441654 9 0.320442433298919 1.0861546686299246 >10 1.91550440256899 14.20197300699452 >50 0.23151276522902584 6.099553113527956 >100 0.17955403043056684 14.170698056513075 >500 0.024404651432048674 6.370209298132353 >1k 0.013179716457467378 8.417245348458481 >5k 9.108451291416097E-5 0.28753536372560357 >10k+ 9.10842302430452E-5 0.5220868033872936 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19857 0.3380902404855614 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10582 0.18017177442807122 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8994 0.15313408988906377 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7770 0.13229396024438797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.002337363560148E-4 0.0 0.0 2 9.194174843239319E-4 0.0 0.0 3 0.0010556274820015515 0.0 0.0 4 0.0011067062311306587 0.0 0.0 5 0.001276968728227683 0.0 0.0 6 0.0015834412230023272 0.0 0.0 7 0.001770729969809054 0.0 0.0 8 0.0020942287142934005 0.0 0.0 9 0.0024007012090680444 0.0 0.0 10-11 0.0033711974425210834 0.0 0.0 12-13 0.005286650534862609 0.0 0.0 14-15 0.008802571099916163 0.0 0.0 16-17 0.015255519739893388 0.0 0.0 18-19 0.02215115087232288 0.0 0.0 20-21 0.027718734527395577 0.0 0.0 22-23 0.03549121751987474 0.0 0.0 24-25 0.04406393424870992 1.7026249709702442E-5 0.0 26-27 0.05455210406988663 1.7026249709702442E-5 0.0 28-29 0.06598523074995181 1.7026249709702442E-5 0.0 30-31 0.07945299427032645 1.7026249709702442E-5 0.0 32-33 0.09328682215945969 1.7026249709702442E-5 0.0 34-35 0.10793791003465864 1.7026249709702442E-5 0.0 36-37 0.12332112664737478 1.7026249709702442E-5 0.0 38-39 0.14067087510156157 1.7026249709702442E-5 0.0 40-41 0.15876977854297528 1.7026249709702442E-5 0.0 42-43 0.1778732307172614 1.7026249709702442E-5 0.0 44-45 0.19945400222430926 1.7026249709702442E-5 0.0 46-47 0.2210177474816474 1.7026249709702442E-5 0.0 48-49 0.2436200939712774 1.7026249709702442E-5 0.0 50-51 0.2675249485636996 1.7026249709702442E-5 0.0 52-53 0.2937453731166414 1.7026249709702442E-5 0.0 54-55 0.3214726207688918 1.7026249709702442E-5 0.0 56-57 0.34919986842114226 1.7026249709702442E-5 0.0 58-59 0.3807665353829306 1.7026249709702442E-5 0.0 60-61 0.41513402042196496 1.7026249709702442E-5 0.0 62-63 0.45155316855101846 1.7026249709702442E-5 0.0 64-65 0.49254386472712713 1.7026249709702442E-5 0.0 66-67 0.5369823764694505 1.7026249709702442E-5 0.0 68-69 0.5861967512553454 1.7026249709702442E-5 0.0 70-71 0.6397783590917789 1.7026249709702442E-5 0.0 72-73 0.702826561766807 1.7026249709702442E-5 0.0 74-75 0.7779208361114498 1.7026249709702442E-5 0.0 76-77 0.867283107712823 1.7026249709702442E-5 0.0 78-79 0.9762170533554992 1.7026249709702442E-5 0.0 80-81 1.1113714235511172 1.7026249709702442E-5 0.0 82-83 1.2823915887602233 1.7026249709702442E-5 0.0 84-85 1.4950579607592618 1.7026249709702442E-5 0.0 86-87 1.7375117566254246 1.7026249709702442E-5 0.0 88-89 2.024574326731008 1.7026249709702442E-5 1.7026249709702442E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTGG 270 0.0 56.207634 1 CGCGGAG 360 0.0 50.05992 1 CGAGGGG 1165 0.0 41.929565 1 GGACGGG 870 0.0 41.428898 1 CGGACGG 115 5.638867E-11 41.239292 1 CGACGGG 280 0.0 40.650158 1 CTGACGG 180 0.0 39.52099 1 GTTGACG 205 0.0 39.3282 1 CGGCTCT 205 0.0 39.3282 1 GTTACGG 230 0.0 39.177326 1 TCGAGCG 50 0.0016289213 37.94015 1 CGGAAAG 385 0.0 36.949642 5 CACGGGG 565 0.0 36.93289 1 CGGGAGA 790 0.0 36.614445 4 CGACCGG 300 0.0 36.35931 1 GAACCGG 185 0.0 35.88933 1 CGCAGCC 440 0.0 34.491043 1 GAGCGGC 690 0.0 34.354347 9 CGCGGGG 580 0.0 34.342377 1 GGTACGG 155 3.8198777E-11 33.65658 1 >>END_MODULE