FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873284
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC198570.3380902404855614No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105820.18017177442807122No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG89940.15313408988906377No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA77700.13229396024438797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG2700.056.2076341
CGCGGAG3600.050.059921
CGAGGGG11650.041.9295651
GGACGGG8700.041.4288981
CGGACGG1155.638867E-1141.2392921
CGACGGG2800.040.6501581
CTGACGG1800.039.520991
GTTGACG2050.039.32821
CGGCTCT2050.039.32821
GTTACGG2300.039.1773261
TCGAGCG500.001628921337.940151
CGGAAAG3850.036.9496425
CACGGGG5650.036.932891
CGGGAGA7900.036.6144454
CGACCGG3000.036.359311
GAACCGG1850.035.889331
CGCAGCC4400.034.4910431
GAGCGGC6900.034.3543479
CGCGGGG5800.034.3423771
GGTACGG1553.8198777E-1133.656581
Produced by FastQC (version 0.11.2)