##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3820979 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24510969570888 33.0 33.0 33.0 33.0 33.0 2 32.24733765875185 33.0 33.0 33.0 33.0 33.0 3 32.241509571238154 33.0 33.0 33.0 33.0 33.0 4 36.021667745360546 37.0 37.0 37.0 37.0 37.0 5 35.995947897122704 37.0 37.0 37.0 33.0 37.0 6 35.95656950744822 37.0 37.0 37.0 33.0 37.0 7 35.94358225993914 37.0 37.0 37.0 33.0 37.0 8 35.879907740921894 37.0 37.0 37.0 33.0 37.0 9 35.83601977398986 37.0 37.0 37.0 33.0 37.0 10-11 35.78874982039943 37.0 37.0 37.0 33.0 37.0 12-13 35.83545028643183 37.0 37.0 37.0 33.0 37.0 14-15 37.679161413868016 40.0 37.0 40.0 33.0 40.0 16-17 37.63252022583741 40.0 37.0 40.0 33.0 40.0 18-19 37.59495773203673 40.0 37.0 40.0 33.0 40.0 20-21 37.54706267686894 40.0 37.0 40.0 33.0 40.0 22-23 37.55218322320013 40.0 37.0 40.0 33.0 40.0 24-25 37.37518225041279 40.0 37.0 40.0 33.0 40.0 26-27 37.3050710302255 40.0 37.0 40.0 33.0 40.0 28-29 37.19999259352119 40.0 37.0 40.0 33.0 40.0 30-31 37.24568193125374 40.0 37.0 40.0 33.0 40.0 32-33 37.18999921224378 40.0 37.0 40.0 33.0 40.0 34-35 37.088540921057145 40.0 37.0 40.0 33.0 40.0 36-37 37.02357288537833 40.0 37.0 40.0 33.0 40.0 38-39 36.98882132039982 40.0 37.0 40.0 33.0 40.0 40-41 36.87428797698182 40.0 37.0 40.0 33.0 40.0 42-43 36.78010831778976 40.0 37.0 40.0 33.0 40.0 44-45 36.694743546091196 40.0 37.0 40.0 33.0 40.0 46-47 36.58665619465587 40.0 37.0 40.0 33.0 40.0 48-49 36.442130276036586 38.5 37.0 40.0 33.0 40.0 50-51 35.25482579202869 37.0 35.0 38.5 30.0 38.5 52-53 35.690173513123206 37.0 37.0 38.5 33.0 40.0 54-55 36.48214632951398 37.0 37.0 40.0 33.0 40.0 56-57 36.50462852583068 37.0 37.0 40.0 33.0 40.0 58-59 36.35346883612812 37.0 37.0 40.0 33.0 40.0 60-61 36.098657569172715 37.0 37.0 40.0 33.0 40.0 62-63 35.839113483743304 37.0 37.0 40.0 33.0 40.0 64-65 35.572219972944104 37.0 37.0 40.0 33.0 40.0 66-67 35.31948382862089 37.0 37.0 38.5 27.0 40.0 68-69 35.09005650122651 37.0 33.0 37.0 27.0 40.0 70-71 34.78887727464611 37.0 33.0 37.0 27.0 40.0 72-73 34.529473074832396 37.0 33.0 37.0 27.0 40.0 74-75 34.2453733715888 37.0 33.0 37.0 27.0 37.0 76-77 34.01911787528798 37.0 33.0 37.0 27.0 37.0 78-79 33.78707721241075 37.0 33.0 37.0 27.0 37.0 80-81 33.575078795251166 37.0 33.0 37.0 27.0 37.0 82-83 33.38002564264289 37.0 33.0 37.0 27.0 37.0 84-85 33.22044167214737 37.0 33.0 37.0 27.0 37.0 86-87 33.02134937145689 37.0 33.0 37.0 27.0 37.0 88-89 32.898710644575644 37.0 33.0 37.0 27.0 37.0 90-91 32.7880477751906 37.0 33.0 37.0 27.0 37.0 92-93 32.63214257393197 33.0 33.0 37.0 27.0 37.0 94-95 32.50150406479596 33.0 33.0 37.0 24.5 37.0 96-97 32.33375556369192 33.0 33.0 37.0 22.0 37.0 98-99 32.20708213785001 33.0 33.0 37.0 22.0 37.0 100-101 30.856352782886272 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13602836315254052 2306 2 0.15843952595982813 2306 3 0.17079703302706406 2306 4 0.2701141786832082 2306 5 0.3109432266936736 2306 6 0.2775000927112288 2306 7 0.25215567840465525 2306 8 0.22599581966257176 2306 9 0.27051434200246405 2306 10-11 0.27930852468246314 2306 12-13 0.19478612060805034 2306 14-15 0.48179330093292805 2306 16-17 0.5342954393906894 2306 18-19 0.4798851280289895 2306 20-21 0.539560751416289 2306 22-23 0.41082970448672285 2306 24-25 0.34930711919182755 2306 26-27 0.4378164379174265 2306 28-29 0.37850838048601787 2306 30-31 0.5124604752210686 2306 32-33 0.5636054403779767 2306 34-35 0.4556706095291929 2306 36-37 0.5196826078391936 2306 38-39 0.3816787452742503 2306 40-41 0.4759703710755261 2306 42-43 0.5575628021933667 2306 44-45 0.5683806577057737 2306 46-47 0.4139635803878221 2306 48-49 0.5276533740263147 2306 50-51 0.49807032721366085 2306 52-53 0.560250111825674 2306 54-55 0.666487095781001 2306 56-57 0.6588276148709511 2306 58-59 0.6601225822706382 2306 60-61 0.5361985836862573 2306 62-63 0.5617404624306914 2306 64-65 0.5390301366191466 2306 66-67 0.5296361672654655 2306 68-69 0.4407895293893631 2306 70-71 0.48502652123261214 2306 72-73 0.5372129135801131 2306 74-75 0.5319274035320873 2306 76-77 0.43323846617219175 2306 78-79 0.4855264443679985 2306 80-81 0.44502264022527527 2306 82-83 0.31115065628515737 2306 84-85 0.4751169484343194 2306 86-87 0.4357034612646089 2306 88-89 0.42797990219315096 2306 90-91 0.4068536081725611 2306 92-93 0.23301017950379332 2306 94-95 0.27411051662496533 2306 96-97 0.3473994822891626 2306 98-99 0.42455838686377234 2306 100-101 0.2942035323584591 2307 1 -0.028839259041802734 2307 2 -0.0733196649897323 2307 3 -0.020042187432288472 2307 4 -0.014584307648419781 2307 5 0.05530476177673194 2307 6 -0.060400636134588126 2307 7 0.01461142090018086 2307 8 0.03883510327516859 2307 9 0.04940462472804086 2307 10-11 0.04751678110758206 2307 12-13 -0.016851643882027645 2307 14-15 0.07395185001590932 2307 16-17 0.13642295726736364 2307 18-19 0.02696179319337233 2307 20-21 -0.026229998930332954 2307 22-23 -0.012909598933028121 2307 24-25 -0.11495474859398769 2307 26-27 0.03738033607996272 2307 28-29 -0.13470126818101846 2307 30-31 0.050037514999004884 2307 32-33 0.010809847325980115 2307 34-35 0.10398651352478794 2307 36-37 0.1627152839003827 2307 38-39 0.08160112419944454 2307 40-41 0.03701360521041863 2307 42-43 0.09322428933737115 2307 44-45 -0.07998904136902496 2307 46-47 0.09014573730066644 2307 48-49 -0.05666429826003139 2307 50-51 -0.18185182206337203 2307 52-53 -0.06912102542067089 2307 54-55 -0.0656880453808455 2307 56-57 0.04262346914772053 2307 58-59 0.0777433700212029 2307 60-61 0.11837478697079717 2307 62-63 0.12939348704036036 2307 64-65 0.17817687891660228 2307 66-67 0.025642930403492414 2307 68-69 -0.048941236860393644 2307 70-71 -0.05215982962957355 2307 72-73 -0.019753089881831443 2307 74-75 -0.11029409785010813 2307 76-77 -0.16756886190028553 2307 78-79 -0.22880675787451565 2307 80-81 -0.1135033057098056 2307 82-83 -0.08972688670927909 2307 84-85 -0.011006619494168035 2307 86-87 0.00894468655231151 2307 88-89 0.057827444527355 2307 90-91 -9.912300546730535E-4 2307 92-93 -0.12308872995455289 2307 94-95 -0.12749070510319171 2307 96-97 -0.025697696196253617 2307 98-99 -0.03610914289718892 2307 100-101 -0.11661605318404256 2201 1 0.17118556075066493 2201 2 0.0927168253859918 2201 3 0.08395855188068424 2201 4 0.07197762301458965 2201 5 0.12995741670240335 2201 6 0.21254631816002956 2201 7 0.16854060527955284 2201 8 0.06845620537956876 2201 9 0.12272651631307951 2201 10-11 0.10951952712718338 2201 12-13 0.09372422811120629 2201 14-15 0.12123962758833073 2201 16-17 0.2814700884690069 2201 18-19 0.23792844489775433 2201 20-21 0.262981312558054 2201 22-23 0.20047988698042474 2201 24-25 0.143255585945127 2201 26-27 0.20259143633608545 2201 28-29 0.08434396193013782 2201 30-31 0.23671931997880336 2201 32-33 0.29035305752850604 2201 34-35 0.3354466566346517 2201 36-37 0.4087787063913382 2201 38-39 0.3063517380932481 2201 40-41 0.30430771975112236 2201 42-43 0.2576369315891114 2201 44-45 0.2695723876173233 2201 46-47 0.26057110629704994 2201 48-49 0.27736495741023504 2201 50-51 0.28409621479666214 2201 52-53 0.27247325018463897 2201 54-55 0.4666265322866181 2201 56-57 0.42602114031186744 2201 58-59 0.4737162266737016 2201 60-61 0.4869991367714448 2201 62-63 0.5203004656266899 2201 64-65 0.3920524631058697 2201 66-67 0.3385600481390796 2201 68-69 0.3539972681431749 2201 70-71 0.3544504204166259 2201 72-73 0.2798004178941582 2201 74-75 0.3817551192702737 2201 76-77 0.3581454992540998 2201 78-79 0.3656998827401665 2201 80-81 0.2308813482653207 2201 82-83 0.31076158792964037 2201 84-85 0.31822502310082257 2201 86-87 0.2767626571382209 2201 88-89 0.2774371928969046 2201 90-91 0.12495311024052569 2201 92-93 0.10284584084786985 2201 94-95 0.09317969333368836 2201 96-97 0.13498458405616987 2201 98-99 0.21508458350420057 2201 100-101 1.5446935180207788E-4 1101 1 0.17912736176697308 1101 2 0.11573418958383996 1101 3 0.08694866441993554 1101 4 0.2222544098033552 1101 5 0.19815313951188784 1101 6 0.21348265719861104 1101 7 0.1841223816063291 1101 8 0.24279752658035392 1101 9 0.21254712897073347 1101 10-11 0.26447655335787346 1101 12-13 0.1826243208910725 1101 14-15 0.2993116503834443 1101 16-17 0.2809445623606024 1101 18-19 0.27635842715699965 1101 20-21 0.24861460305996275 1101 22-23 0.21830381098633467 1101 24-25 0.0851029921850639 1101 26-27 0.22071097484360536 1101 28-29 0.09852152084923915 1101 30-31 0.26434015701621405 1101 32-33 0.2916669213245555 1101 34-35 0.253768253379981 1101 36-37 0.2936724753888882 1101 38-39 0.1934287453797623 1101 40-41 0.3407813667761559 1101 42-43 0.24956372478317945 1101 44-45 0.2922234848653673 1101 46-47 0.2921713842484408 1101 48-49 0.2406714919852888 1101 50-51 0.35551273815273277 1101 52-53 0.5367235966533812 1101 54-55 0.5472426255167946 1101 56-57 0.6139747037962024 1101 58-59 0.542078196246571 1101 60-61 0.5273982648736535 1101 62-63 0.457921147383999 1101 64-65 0.5597757461931465 1101 66-67 0.4448481160275435 1101 68-69 0.3893470754017443 1101 70-71 0.37759123229866276 1101 72-73 0.3708225103712266 1101 74-75 0.355138556317776 1101 76-77 0.37782472937533385 1101 78-79 0.40349418706588125 1101 80-81 0.2738793800899728 1101 82-83 0.2922820907266228 1101 84-85 0.23681544852949798 1101 86-87 0.11793663296887047 1101 88-89 0.16481136748609515 1101 90-91 0.19760983639240948 1101 92-93 0.19107628817103262 1101 94-95 0.21846618512907412 1101 96-97 0.07448250524375766 1101 98-99 0.02651484914964186 1101 100-101 0.08935308813525822 2304 1 0.1546592755342786 2304 2 0.12045426366656642 2304 3 0.15264864772732523 2304 4 0.24560527331955484 2304 5 0.20221997842183725 2304 6 0.16706272826749569 2304 7 0.2272684588955869 2304 8 0.23366096866740094 2304 9 0.27677821541269765 2304 10-11 0.2659383479229973 2304 12-13 0.2493733713002868 2304 14-15 0.25102469874719446 2304 16-17 0.2658587030493109 2304 18-19 0.26546257211239066 2304 20-21 0.3124439904552432 2304 22-23 0.2077700409159391 2304 24-25 0.1131655481873608 2304 26-27 0.060680312316328866 2304 28-29 0.0821656290808832 2304 30-31 0.1361384312190026 2304 32-33 0.17022526200134536 2304 34-35 0.3498091312207734 2304 36-37 0.39353037644652744 2304 38-39 0.2012980058829612 2304 40-41 0.2565731859143554 2304 42-43 0.21006214216128427 2304 44-45 0.307998179078254 2304 46-47 0.2903126107989351 2304 48-49 0.3342484892138913 2304 50-51 0.1576512540066446 2304 52-53 0.31516857403012466 2304 54-55 0.3678028320252267 2304 56-57 0.3739735074335684 2304 58-59 0.3554182307399216 2304 60-61 0.44649666799421794 2304 62-63 0.41481106684656055 2304 64-65 0.36880239049477836 2304 66-67 0.4806309165677831 2304 68-69 0.3938317148466268 2304 70-71 0.4518509432165132 2304 72-73 0.4196929534735929 2304 74-75 0.46502552298795763 2304 76-77 0.32930437232990784 2304 78-79 0.2904893105647446 2304 80-81 0.41139253037393786 2304 82-83 0.3813105278076563 2304 84-85 0.3847222638792118 2304 86-87 0.41926919213676683 2304 88-89 0.2925112562137855 2304 90-91 0.2383280739349729 2304 92-93 0.2808592012893385 2304 94-95 0.39258428235488907 2304 96-97 0.3948451707538183 2304 98-99 0.29248801074788133 2304 100-101 -0.03718215568450489 2202 1 -0.013352934241765979 2202 2 -0.02440146873681215 2202 3 -0.04255928572830214 2202 4 0.055970055195182056 2202 5 -0.016709297204087648 2202 6 0.02792642305014681 2202 7 0.05659232579814244 2202 8 0.024026798944973393 2202 9 0.04706408853464694 2202 10-11 0.058588961693089914 2202 12-13 -0.08658208079648233 2202 14-15 0.08141419939624939 2202 16-17 0.10766157272353638 2202 18-19 0.03097914237896049 2202 20-21 0.052223742302849985 2202 22-23 0.04777291234555037 2202 24-25 -0.08024858725023165 2202 26-27 0.023755909746405734 2202 28-29 -0.08019499365054372 2202 30-31 0.12972325003670448 2202 32-33 0.05655207948704799 2202 34-35 0.14931288763206396 2202 36-37 0.164835220570815 2202 38-39 0.06899918169553843 2202 40-41 0.00884482412131149 2202 42-43 0.05150101022215381 2202 44-45 0.10734266213678012 2202 46-47 0.16135800362395258 2202 48-49 0.04227225073675811 2202 50-51 -0.011001753211942855 2202 52-53 0.0973637940419465 2202 54-55 0.12094950740334554 2202 56-57 0.3111270446104939 2202 58-59 0.19439858818056877 2202 60-61 0.22702128929525145 2202 62-63 0.20982863724188405 2202 64-65 0.3162459686342558 2202 66-67 0.2623535504787853 2202 68-69 0.31588761979345037 2202 70-71 0.333703975139386 2202 72-73 0.2929949872042741 2202 74-75 0.18276008742325445 2202 76-77 0.10645208451474275 2202 78-79 0.16763129760530404 2202 80-81 -0.022559113173258538 2202 82-83 0.055867881956785936 2202 84-85 0.14267105500010047 2202 86-87 0.16145708782898538 2202 88-89 0.20249797726099672 2202 90-91 0.04070881599903231 2202 92-93 0.15643909358416153 2202 94-95 0.19507240107006396 2202 96-97 0.16188196410615063 2202 98-99 0.06449170898172696 2202 100-101 0.1137764114221973 1102 1 -0.0655542894271548 1102 2 -0.15011257713241122 1102 3 -0.14875938756988205 1102 4 -0.10422250587204474 1102 5 -0.04749492422660495 1102 6 -0.050947396059441985 1102 7 -0.02127513457403296 1102 8 0.028849480088808832 1102 9 0.04183412983049095 1102 10-11 0.0388419175991217 1102 12-13 -0.02287239765043836 1102 14-15 -0.100997919337658 1102 16-17 -0.05876760166434991 1102 18-19 -0.04896842192962225 1102 20-21 -0.017853961462421353 1102 22-23 -0.048569829844055334 1102 24-25 -0.1105157971311641 1102 26-27 -0.04907957201427138 1102 28-29 -0.05435450472811709 1102 30-31 0.026780813904430545 1102 32-33 -0.03395818322695021 1102 34-35 0.1824842247007794 1102 36-37 0.09591791759151391 1102 38-39 0.07658992263298359 1102 40-41 0.10089887294550692 1102 42-43 0.17986774435911457 1102 44-45 0.21760337155422604 1102 46-47 0.12355379184435833 1102 48-49 0.1736664566549848 1102 50-51 0.1820381203411685 1102 52-53 0.1523099085430104 1102 54-55 0.28012195135570295 1102 56-57 0.3543734713527513 1102 58-59 0.41858957339578495 1102 60-61 0.35539190684519895 1102 62-63 0.3393710839074515 1102 64-65 0.3077451776015181 1102 66-67 0.33749511382693953 1102 68-69 0.38140715831676175 1102 70-71 0.2517684362444541 1102 72-73 0.2889006494127315 1102 74-75 0.3334129150361065 1102 76-77 0.2620325563767878 1102 78-79 0.23675349685086644 1102 80-81 0.18067778922125655 1102 82-83 0.23716723643738646 1102 84-85 0.27569337256771576 1102 86-87 0.04039864105807567 1102 88-89 0.01718269294634922 1102 90-91 0.11476646330465456 1102 92-93 0.17274314070704833 1102 94-95 0.215315375495166 1102 96-97 0.2522293083990377 1102 98-99 0.16723712662837187 1102 100-101 0.19223825589098809 2305 1 -0.0671099627504077 2305 2 -0.05287641409089616 2305 3 -0.05648289994539368 2305 4 -0.008258179716300162 2305 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-0.5256750419328498 1213 82-83 -0.5917775708422042 1213 84-85 -0.5471480412561078 1213 86-87 -0.5596729877386935 1213 88-89 -0.4505909910544119 1213 90-91 -0.3923896731807659 1213 92-93 -0.38405234574275937 1213 94-95 -0.47519483047612 1213 96-97 -0.45187341033499706 1213 98-99 -0.4434950350133775 1213 100-101 -0.29712083697060976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4065.0 3 308.0 4 330.0 5 294.0 6 568.0 7 1607.0 8 6824.0 9 7532.0 10 8040.0 11 7067.0 12 6020.0 13 5495.0 14 5627.0 15 5987.0 16 6431.0 17 6969.0 18 7956.0 19 8684.0 20 10065.0 21 11450.0 22 12943.0 23 14446.0 24 16496.0 25 19545.0 26 23331.0 27 28823.0 28 35448.0 29 43625.0 30 55108.0 31 72157.0 32 100389.0 33 145667.0 34 220856.0 35 371367.0 36 732027.0 37 1356669.0 38 458623.0 39 2140.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.89247340597148 32.217609110667986 15.656285067211007 19.233632416149526 2 31.339161917004994 24.602097503066236 29.665881797372545 14.392858782556225 3 32.78051475159231 23.337570382137034 24.07904517939084 19.80286968687982 4 28.832840550570317 22.84341205880031 28.579739140310238 19.74400825031913 5 30.883377470380612 21.076165784625196 28.499635921820772 19.54082082317342 6 28.434935054167877 21.42008204678283 30.323280905809913 19.82170199323938 7 31.813927574263502 21.935105801424676 24.481695257061734 21.769271367250088 8 27.83771244108275 25.424187131484437 25.057471445248495 21.68062898218431 9 26.269880941203866 23.237262114503938 28.32082461552018 22.17203232877201 10-11 22.25203054836278 29.041804057819004 31.00607689534236 17.70008849847586 12-13 22.63536193673263 28.315225010560475 30.078302917025297 18.971110135681606 14-15 22.384907443883804 28.112821995040804 30.571962649178513 18.93030791189688 16-17 22.53450031396112 27.79026715284739 30.798969410167516 18.876263123023975 18-19 22.091961875638845 27.810025970041025 30.88920345209442 19.208808702225713 20-21 22.138190993648337 28.274466601509285 30.32489180718904 19.262450597653338 22-23 22.21836121538087 28.283376696474484 30.017133345296482 19.48112874284816 24-25 21.661635325838997 28.475876591619393 30.273954568129984 19.588533514411623 26-27 21.51922921603738 28.6803023408367 30.47882451899874 19.321643924127187 28-29 21.380556282849025 29.121442784257372 30.09626004875927 19.401740884134323 30-31 21.239407627951916 29.45062100345126 30.086537464342022 19.2234339042548 32-33 20.763444382479516 29.52995064375371 30.41814767365773 19.28845730010904 34-35 20.862037284039836 29.777333654342108 30.45862355797249 18.902005503645565 36-37 20.80117358585886 29.338608760096502 30.949675334271127 18.91054231977351 38-39 21.03312876183346 28.889107799001113 30.848292008576898 19.22947143058853 40-41 21.008137894182944 29.29137507214608 30.030397899599976 19.670089134071 42-43 21.399173114688082 28.887022043177936 30.053396109817143 19.660408732316842 44-45 20.889008227159348 29.030990175436017 30.342044268076023 19.737957329328612 46-47 21.09535797853967 29.28424526010012 30.07277781697557 19.54761894438464 48-49 20.875251753587158 29.685815990381432 29.83405974921145 19.604872506819962 50-51 20.993755748163707 29.367957246999655 29.518390116003346 20.11989688883329 52-53 21.203318280663005 29.13110909147296 29.507219972761167 20.158352655102867 54-55 21.555683416946803 29.10727351784783 29.47300526377323 19.864037801432136 56-57 21.578208859137998 28.869556648951892 29.57157403088808 19.980660461022026 58-59 21.54890174771282 29.31062117719024 29.47508180990253 19.665395265194412 60-61 21.08787613115784 29.582453244775543 29.139392895170484 20.190277728896127 62-63 21.131977139916604 29.488234224619287 29.071018667842885 20.308769967621227 64-65 20.917748046897568 29.369465947158822 29.281882289665283 20.430903716278323 66-67 21.279698900556287 29.03785542814219 29.28014802830293 20.402297642998597 68-69 21.29373010447506 29.150395921110444 29.706649914659835 19.84922405975466 70-71 21.198487889847055 29.868363136952507 29.296786339286967 19.636362633913468 72-73 20.955094369985368 30.07786099687645 29.039714371857105 19.927330261281075 74-75 21.119105257692752 29.61010189878594 28.943689641053673 20.327103202467637 76-77 21.206220649279842 29.304173859320294 28.977936227304003 20.51166926409586 78-79 20.885366698759917 28.99307240071914 29.74957679843106 20.371984102089886 80-81 20.844192925443792 29.431005737961396 29.40059515970215 20.324206176892663 82-83 20.400882175605897 29.710138360993582 29.264204449568098 20.624775013832423 84-85 20.45383157680396 29.696698994689356 29.41900861460246 20.430460813904226 86-87 20.641305495898322 29.517995523911967 29.875825078389344 19.964873901800367 88-89 20.76677244946242 29.961520949748927 29.59890463705922 19.672801963729437 90-91 21.087393956363037 29.718365645578192 29.070763698044562 20.12347670001421 92-93 20.815015049397 29.79599414515171 29.08749605448979 20.301494750961496 94-95 20.39985371461696 29.942310211649115 29.0744824156936 20.583353658040327 96-97 20.530187455525592 30.332202490829406 28.942211497307248 20.19539855633775 98-99 20.87693748882878 30.075050913193646 28.817255025359085 20.230756572618493 100-101 20.953826317769423 30.095946917236034 28.446633517085136 20.503593247909414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3136.0 1 1949.0 2 598.0 3 380.0 4 305.5 5 315.0 6 391.5 7 541.0 8 734.5 9 957.0 10 1192.5 11 1286.5 12 1223.5 13 1099.5 14 953.0 15 793.0 16 670.5 17 656.5 18 702.0 19 741.5 20 928.0 21 1252.5 22 1707.0 23 2421.0 24 3410.5 25 5006.5 26 7924.5 27 12266.0 28 17042.5 29 22089.0 30 28014.0 31 32622.5 32 39623.5 33 54850.0 34 76604.5 35 108592.5 36 150613.5 37 200690.5 38 250987.0 39 270123.0 40 275739.5 41 265065.0 42 233569.5 43 218739.5 44 206274.0 45 196880.5 46 201203.0 47 187819.0 48 154007.0 49 125336.0 50 100034.0 51 80868.0 52 66546.0 53 52862.5 54 43182.5 55 33499.0 56 23093.5 57 14989.0 58 9983.5 59 7163.0 60 5327.5 61 4007.5 62 2881.5 63 2008.5 64 1434.0 65 1061.0 66 816.5 67 645.5 68 470.5 69 326.0 70 244.0 71 187.0 72 155.0 73 119.0 74 103.5 75 89.5 76 72.0 77 57.5 78 46.5 79 38.5 80 29.5 81 27.0 82 23.0 83 20.5 84 22.5 85 17.5 86 14.0 87 10.5 88 7.0 89 6.5 90 6.0 91 5.5 92 4.5 93 5.0 94 4.0 95 2.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2265649719613743 2 0.2434978051436556 3 0.2244712677039052 4 0.21664604804161447 5 0.22536109201332954 6 0.24849652405836306 7 0.22881570403815357 8 0.2150234272420759 9 0.20688415194116483 10-11 0.19246376386784644 12-13 0.22019225962770275 14-15 0.21630582109977572 16-17 0.21996980355034665 18-19 0.20757769147645147 20-21 0.20015812701404534 22-23 0.1697863296291343 24-25 0.5053286081917749 26-27 0.35819354149813437 28-29 0.4928187252533971 30-31 0.3199180105412775 32-33 0.37074268139133976 34-35 0.37240455914570586 36-37 0.3801512648983415 38-39 0.3727186147843262 40-41 0.3785940723568489 42-43 0.4339725499669064 44-45 0.3874661441478741 46-47 0.40545891511049914 48-49 0.4036269238852137 50-51 0.417680913713475 52-53 0.4218028939703673 54-55 0.3866548337481049 56-57 0.4005256244538376 58-59 0.39793466543521966 60-61 0.35585120986009083 62-63 0.31815144757403796 64-65 0.3452256607534352 66-67 0.35590355246652755 68-69 0.3474502215269961 70-71 0.3174448223871421 72-73 0.30595562027428047 74-75 0.31799441975472775 76-77 0.3246681020754105 78-79 0.33513662336275596 80-81 0.34267395868964473 82-83 0.36117707006502786 84-85 0.3910385270371808 86-87 0.3919676083014327 88-89 0.3860790650773009 90-91 0.358599196698019 92-93 0.33637976026562827 94-95 0.313689240375307 96-97 0.3299939622803475 98-99 0.36020873184594837 100-101 0.37673590982834504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3820979.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.29617225946368 #Duplication Level Percentage of deduplicated Percentage of total 1 87.77313603081718 38.88013953374416 2 5.7696470403475 5.111465583510753 3 1.7965920896084502 2.387464580438572 4 0.8993692129982717 1.593544543353189 5 0.6052979520118832 1.340619117530948 6 0.3756325454157307 0.9983450362797615 7 0.3151914985626454 0.9773243840534608 8 0.2855347500616082 1.0118477179833525 9 0.20105507820999285 0.8015373340226808 >10 1.5826669579197994 14.273724107523005 >50 0.21036740120321454 6.5526852155697215 >100 0.15297397104781954 13.328193641177613 >500 0.023714651653336815 7.292517727646265 >1k 0.008820820142530971 5.450591477166479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.945095222978196E-4 0.0 0.0 2 0.001099194735171274 0.0 0.0 3 0.0015441068898834566 0.0 0.0 4 0.0018058199220670932 0.0 0.0 5 0.0021722181671241848 0.0 0.0 6 0.0030096998701118222 0.0 0.0 7 0.004030380695628005 0.0 0.0 8 0.005181918037236007 0.0 0.0 9 0.00570534410160328 0.0 0.0 10-11 0.0075373353268887375 0.0 0.0 12-13 0.010455435635736286 0.0 0.0 14-15 0.015349469337570292 0.0 0.0 16-17 0.02360651550296403 0.0 0.0 18-19 0.03308052726801168 0.0 0.0 20-21 0.042227397742829784 0.0 0.0 22-23 0.05397831288787507 0.0 0.0 24-25 0.06775750403234354 0.0 0.0 26-27 0.08294994555060364 0.0 0.0 28-29 0.09926775310725341 0.0 0.0 30-31 0.12058427957861062 0.0 0.0 32-33 0.13954538876031508 0.0 0.0 34-35 0.16200036692167114 0.0 0.0 36-37 0.1872949314822196 0.0 0.0 38-39 0.21484022811954737 0.0 0.0 40-41 0.24606259285905524 0.0 0.0 42-43 0.27916929143028524 0.0 0.0 44-45 0.3168690537163381 0.0 0.0 46-47 0.3578271432530773 0.0 0.0 48-49 0.4035091530207311 0.0 0.0 50-51 0.4518213787618304 0.0 0.0 52-53 0.502672220915111 0.0 0.0 54-55 0.5564804203320668 0.0 0.0 56-57 0.6155228803926951 0.0 0.0 58-59 0.6803884554194095 0.0 0.0 60-61 0.7471514499294553 0.0 0.0 62-63 0.8162044334711078 0.0 0.0 64-65 0.8913160737078116 0.0 0.0 66-67 0.9711908911302574 0.0 0.0 68-69 1.0553316309772967 0.0 0.0 70-71 1.144222462358469 0.0 0.0 72-73 1.2370259035707865 0.0 0.0 74-75 1.3381649048581528 0.0 0.0 76-77 1.448202149239763 0.0 0.0 78-79 1.5654103307032046 0.0 0.0 80-81 1.6899595627193973 0.0 0.0 82-83 1.8240482347586835 0.0 0.0 84-85 1.9723348387939321 0.0 0.0 86-87 2.128276549020552 0.0 0.0 88-89 2.2950008361731378 0.0 2.617130321836367E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGGCG 175 0.0 59.60926 1 ACGCGGA 45 1.5334756E-5 52.67942 2 CGGGAGA 460 0.0 46.380188 4 GGACCGG 205 0.0 43.94696 1 CGGGCTG 365 0.0 42.869675 1 CGAGGGG 510 0.0 42.767784 1 GAGCGGC 460 0.0 39.156246 9 CGGACGG 50 0.0016303231 37.933167 1 TCCGGGC 100 3.0842784E-8 37.929188 2 GCGTGCC 75 7.016357E-6 37.928688 3 CGCAGCC 145 1.6370905E-11 35.971107 1 CGAGTTG 95 8.827683E-7 34.938442 1 ACGGCGA 95 8.827683E-7 34.938442 1 CGCGGAG 125 5.7571015E-9 34.13985 1 CGATTGC 70 2.0755731E-4 33.868896 1 ACGAGCG 70 2.0755731E-4 33.868896 1 CGAGCCC 170 3.6379788E-12 33.470444 1 GCGCACC 85 1.6541728E-5 33.46693 2 TCGGAAA 235 0.0 32.27973 4 TTGTGTT 24355 0.0 32.104183 1 >>END_MODULE