FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820979
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCG1750.059.609261
ACGCGGA451.5334756E-552.679422
CGGGAGA4600.046.3801884
GGACCGG2050.043.946961
CGGGCTG3650.042.8696751
CGAGGGG5100.042.7677841
GAGCGGC4600.039.1562469
CGGACGG500.001630323137.9331671
TCCGGGC1003.0842784E-837.9291882
GCGTGCC757.016357E-637.9286883
CGCAGCC1451.6370905E-1135.9711071
CGAGTTG958.827683E-734.9384421
ACGGCGA958.827683E-734.9384421
CGCGGAG1255.7571015E-934.139851
CGATTGC702.0755731E-433.8688961
ACGAGCG702.0755731E-433.8688961
CGAGCCC1703.6379788E-1233.4704441
GCGCACC851.6541728E-533.466932
TCGGAAA2350.032.279734
TTGTGTT243550.032.1041831
Produced by FastQC (version 0.11.2)