FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047836
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT100530.32984058197357075Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56510.18541023860863906No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC54910.18016061231641073No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG53570.1757640502966695No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG47200.1548639756207355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43850.14387257057138245No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA38520.12638475298539684No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA33190.10889693539941127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA37000.086.512544
GAGCGGC28950.086.305629
AAGCGGC11500.084.9271249
AGAGCGG30450.084.549498
GTTCGGA7450.084.0250852
CGGAAAG18450.082.506035
TCGGAGA11600.081.761534
GAGAGCG34000.080.4654857
ATTCGGA8100.079.624222
TCGGGAG41550.077.152973
AAAGCGG13750.075.860988
GGATCGG17900.074.978881
AAGGGGC30050.074.9421549
GATCCGG9200.074.745621
TCGGGAA15250.073.387013
TTCGGAG8100.073.181623
ACCGGAA4300.072.786783
CGGAGAG19500.072.470075
ATCGGGA55150.071.113372
ATCCGGA9500.070.3860862
Produced by FastQC (version 0.11.2)