##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3047836 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93400826028697 33.0 33.0 33.0 27.0 33.0 2 30.373019414430434 33.0 33.0 33.0 22.0 33.0 3 30.233239255655487 33.0 33.0 33.0 22.0 33.0 4 33.55722683241487 37.0 37.0 37.0 15.0 37.0 5 33.6178675624279 37.0 37.0 37.0 15.0 37.0 6 34.21486129831133 37.0 37.0 37.0 27.0 37.0 7 33.69899200613156 37.0 37.0 37.0 22.0 37.0 8 33.90359815948102 37.0 37.0 37.0 22.0 37.0 9 33.29737492437257 37.0 37.0 37.0 15.0 37.0 10-11 33.34802151428095 37.0 37.0 37.0 15.0 37.0 12-13 33.364960417817755 37.0 37.0 37.0 15.0 37.0 14-15 34.93301690117185 40.0 37.0 40.0 15.0 40.0 16-17 34.82325508984079 40.0 37.0 40.0 15.0 40.0 18-19 34.8801849902685 40.0 37.0 40.0 15.0 40.0 20-21 34.81342729726927 40.0 37.0 40.0 15.0 40.0 22-23 35.67968535708614 40.0 37.0 40.0 24.5 40.0 24-25 34.997377811667036 40.0 37.0 40.0 18.5 40.0 26-27 34.75916748801445 40.0 37.0 40.0 18.5 40.0 28-29 34.42640352039939 40.0 37.0 40.0 15.0 40.0 30-31 34.45827301731458 40.0 37.0 40.0 15.0 40.0 32-33 34.2390400927084 37.0 37.0 40.0 15.0 40.0 34-35 34.42276208431163 37.0 37.0 40.0 15.0 40.0 36-37 34.23864226946594 37.0 37.0 40.0 15.0 40.0 38-39 34.123292723099276 37.0 37.0 40.0 15.0 40.0 40-41 33.936825505046855 37.0 37.0 40.0 15.0 40.0 42-43 33.83364885774694 37.0 33.0 40.0 15.0 40.0 44-45 33.70989465968641 37.0 33.0 40.0 15.0 40.0 46-47 33.624315251870506 37.0 33.0 40.0 10.5 40.0 48-49 33.50755831350506 37.0 33.0 40.0 10.5 40.0 50-51 32.566740303612136 37.0 33.0 38.5 15.0 38.5 52-53 32.98093811478046 37.0 33.0 38.5 15.0 40.0 54-55 33.64614647901003 37.0 33.0 40.0 15.0 40.0 56-57 33.959090646609596 37.0 33.0 40.0 18.5 40.0 58-59 33.39929231756564 37.0 33.0 40.0 15.0 40.0 60-61 33.14833212810663 37.0 33.0 40.0 10.5 40.0 62-63 32.77583488744145 37.0 33.0 40.0 6.0 40.0 64-65 32.545700293585355 37.0 33.0 38.5 6.0 40.0 66-67 32.40405668152748 37.0 33.0 37.0 10.5 40.0 68-69 32.49169984867952 37.0 33.0 37.0 15.0 40.0 70-71 32.09900188199103 37.0 33.0 37.0 15.0 40.0 72-73 32.34878041338182 37.0 33.0 37.0 15.0 37.0 74-75 32.47552575007317 37.0 33.0 37.0 22.0 37.0 76-77 32.22071545188127 37.0 33.0 37.0 22.0 37.0 78-79 31.75217416553909 37.0 33.0 37.0 18.5 37.0 80-81 31.23990414838594 37.0 33.0 37.0 15.0 37.0 82-83 30.879013995503694 33.0 33.0 37.0 15.0 37.0 84-85 30.631032476813054 33.0 30.0 37.0 15.0 37.0 86-87 30.409308604531216 33.0 27.0 37.0 15.0 37.0 88-89 30.28710747559908 33.0 27.0 37.0 10.5 37.0 90-91 30.176781821594076 33.0 27.0 37.0 6.0 37.0 92-93 30.00690309452346 33.0 27.0 37.0 6.0 37.0 94-95 29.87617804895014 33.0 27.0 37.0 6.0 37.0 96-97 29.719963606965727 33.0 27.0 37.0 6.0 37.0 98-99 29.601928712699767 33.0 27.0 37.0 6.0 37.0 100-101 28.39177272005449 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.21519247626092763 2306 2 -0.35211910015197034 2306 3 -0.523015095880563 2306 4 -0.4063873374500844 2306 5 -0.407579780159665 2306 6 -0.5536836775376699 2306 7 -0.07028459311368351 2306 8 -0.6822785886121352 2306 9 -0.44534224307534487 2306 10-11 -0.540063450147791 2306 12-13 -0.5349483465309604 2306 14-15 -0.258664821851724 2306 16-17 -0.18632597458496747 2306 18-19 -0.1551694541188553 2306 20-21 -0.3022186289693991 2306 22-23 -0.4053261388571059 2306 24-25 -0.19631270927582278 2306 26-27 -0.5791934442843569 2306 28-29 -0.5710859057559929 2306 30-31 -0.1503192839763443 2306 32-33 -0.09051820254000376 2306 34-35 -0.34269526069851963 2306 36-37 0.18509092167575147 2306 38-39 -0.2563272666088139 2306 40-41 -0.14189408124146752 2306 42-43 -0.14871850729785052 2306 44-45 -0.20101486329945573 2306 46-47 -0.22054284710854688 2306 48-49 -0.1351694133511927 2306 50-51 -0.13237858327496355 2306 52-53 -0.11620986801842292 2306 54-55 -0.06627790483878471 2306 56-57 -0.025781759161567663 2306 58-59 0.13902607304565606 2306 60-61 -0.02659947061850687 2306 62-63 0.0553076447599139 2306 64-65 -0.005515908017827087 2306 66-67 -0.10356254550085708 2306 68-69 -0.2852378283652399 2306 70-71 -0.15318216804908502 2306 72-73 -0.5114450981410208 2306 74-75 -0.27421883926133717 2306 76-77 -0.030141169409006352 2306 78-79 -1.5515598302684452E-4 2306 80-81 0.0319417952481551 2306 82-83 0.016631335782612666 2306 84-85 -0.01265540389998776 2306 86-87 -0.06763224968363701 2306 88-89 -0.04994459105429172 2306 90-91 -0.12381531136893287 2306 92-93 -0.18839134960800763 2306 94-95 -0.15943826368051717 2306 96-97 -0.19781463152677858 2306 98-99 -0.15209253365825148 2306 100-101 -0.263985372060521 2307 1 0.08897573180740181 2307 2 0.12903607139893936 2307 3 0.15382353187725784 2307 4 0.33057833792118174 2307 5 0.32100273397350065 2307 6 0.12923723088578498 2307 7 0.5621139413809502 2307 8 0.04980851825305166 2307 9 0.23847693490224486 2307 10-11 0.28899587949856453 2307 12-13 0.1814056284279033 2307 14-15 0.4583447970105965 2307 16-17 0.4887208866053001 2307 18-19 0.4188005413046625 2307 20-21 0.3754475831450179 2307 22-23 0.19404009353414864 2307 24-25 0.33702516442057373 2307 26-27 0.23586995827403712 2307 28-29 0.22054651470638476 2307 30-31 0.479549287991766 2307 32-33 0.46660446620284546 2307 34-35 0.4704308772944614 2307 36-37 0.6968050583540872 2307 38-39 0.5589369696221453 2307 40-41 0.6572749549271677 2307 42-43 0.6920859160476596 2307 44-45 0.5021358132920355 2307 46-47 0.693809524276638 2307 48-49 0.6597074318807259 2307 50-51 0.41201610635769015 2307 52-53 0.47613002355976874 2307 54-55 0.5786784741391315 2307 56-57 0.5286066307173414 2307 58-59 0.7696970767909406 2307 60-61 0.7353263346897023 2307 62-63 0.8512590855770554 2307 64-65 0.9250785802910002 2307 66-67 0.6953147207465449 2307 68-69 0.4670616333437607 2307 70-71 0.5454583285840755 2307 72-73 0.148131378857137 2307 74-75 0.24547550143409325 2307 76-77 0.46831639702863015 2307 78-79 0.432746533290004 2307 80-81 0.7002233865009373 2307 82-83 0.6412044513642599 2307 84-85 0.6593398689629311 2307 86-87 0.649897576116043 2307 88-89 0.7729052761658863 2307 90-91 0.6321383686727167 2307 92-93 0.6348456093600099 2307 94-95 0.5814766246342877 2307 96-97 0.7314410946191607 2307 98-99 0.6301289524748093 2307 100-101 0.6022250913093252 2201 1 -0.1362383857547158 2201 2 -0.07223894947999554 2201 3 -0.23459339703439142 2201 4 -0.10008728184823923 2201 5 -0.08852847404734376 2201 6 -0.30577565053227573 2201 7 0.13240219218087645 2201 8 -0.2950767847119309 2201 9 -0.07451271344996968 2201 10-11 -0.12331606255830962 2201 12-13 -0.16813760363825025 2201 14-15 0.19262554458409653 2201 16-17 0.18319489363228314 2201 18-19 0.08215191764825391 2201 20-21 0.014699148804844242 2201 22-23 -0.05860786921629568 2201 24-25 0.005978968343931967 2201 26-27 -0.2537284094548724 2201 28-29 -0.128642313483752 2201 30-31 0.16861837557360815 2201 32-33 0.32840639296586005 2201 34-35 0.22676588996761637 2201 36-37 0.3992297223646375 2201 38-39 0.19416027183319073 2201 40-41 0.30035102784064094 2201 42-43 0.2652402910437601 2201 44-45 0.17911629226835402 2201 46-47 0.24101952228419776 2201 48-49 0.383155431008781 2201 50-51 0.1463985584715104 2201 52-53 0.1590142254776552 2201 54-55 0.29382835469704105 2201 56-57 0.37234923284015053 2201 58-59 0.6146715881752556 2201 60-61 0.5638212393963613 2201 62-63 0.5618603780925042 2201 64-65 0.4387753702457786 2201 66-67 0.3546360980690011 2201 68-69 0.20531198202024825 2201 70-71 0.26794725042570633 2201 72-73 0.08448932885460891 2201 74-75 -0.10973402727470472 2201 76-77 0.23816110495609877 2201 78-79 0.3199367596572351 2201 80-81 0.3964642061909416 2201 82-83 0.4227441400858858 2201 84-85 0.4413492035653128 2201 86-87 0.255835871659027 2201 88-89 0.3070387777803134 2201 90-91 0.3536223941175969 2201 92-93 0.14159073778195364 2201 94-95 0.1420415048416146 2201 96-97 0.45263562659430434 2201 98-99 0.2963068819647532 2201 100-101 0.23119234938962663 1101 1 -0.019861143345263343 1101 2 -0.05742196558541224 1101 3 -0.08618279023432862 1101 4 0.16700117223874855 1101 5 0.07295813434995324 1101 6 -0.03355771717232159 1101 7 0.3528307826241104 1101 8 -0.19003515316956765 1101 9 0.2055582746151643 1101 10-11 0.18454971084187122 1101 12-13 0.03674134692151654 1101 14-15 0.2270512067696373 1101 16-17 0.24122142542162806 1101 18-19 0.21297730179720986 1101 20-21 0.19227934313467188 1101 22-23 0.01610943560348943 1101 24-25 0.10391942710430868 1101 26-27 -0.04510335954138611 1101 28-29 0.05807227012688543 1101 30-31 0.271930856162804 1101 32-33 0.3158666470439755 1101 34-35 0.18194372985994534 1101 36-37 0.5163260147171513 1101 38-39 0.26207130012489444 1101 40-41 0.4763280750329173 1101 42-43 0.3699246159916214 1101 44-45 0.20401003200835532 1101 46-47 0.388507284237555 1101 48-49 0.3454229081225222 1101 50-51 0.4214412252564941 1101 52-53 0.49901836644811937 1101 54-55 0.6783807601572107 1101 56-57 0.5319179803444811 1101 58-59 0.7195750887742136 1101 60-61 0.7037404904722777 1101 62-63 0.5802350471845941 1101 64-65 0.5905150215169073 1101 66-67 0.5510553417503274 1101 68-69 0.5434384886679808 1101 70-71 0.5737747790057881 1101 72-73 0.2652447601810479 1101 74-75 0.25795161391020827 1101 76-77 0.3760489142348291 1101 78-79 0.5311093937581255 1101 80-81 0.3726903031783806 1101 82-83 0.44874529364194515 1101 84-85 0.4194786680490985 1101 86-87 0.40599367221214067 1101 88-89 0.35598356977049406 1101 90-91 0.5086609199321082 1101 92-93 0.32571159496283997 1101 94-95 0.44848899585718627 1101 96-97 0.3856276256477962 1101 98-99 0.3621728624134555 1101 100-101 0.18662959150329783 2304 1 -0.030187102321530546 2304 2 -0.055806423663124605 2304 3 -0.05824652574898437 2304 4 0.09215485631881393 2304 5 0.07062947970534594 2304 6 -0.08184178484289362 2304 7 0.3588816285062393 2304 8 -0.00290542025762619 2304 9 0.15905195373767356 2304 10-11 0.13564246352204634 2304 12-13 -0.04264647299544322 2304 14-15 0.13476616308121692 2304 16-17 0.08715747301412335 2304 18-19 0.07618948497871969 2304 20-21 0.11989158518709786 2304 22-23 0.013327939662488575 2304 24-25 0.1403867072679077 2304 26-27 -0.0716847408390251 2304 28-29 -0.029424941250695724 2304 30-31 0.2076814603643271 2304 32-33 0.25047306397401314 2304 34-35 0.2142384562651003 2304 36-37 0.3044015554000552 2304 38-39 0.07265629113933869 2304 40-41 0.2632771671813927 2304 42-43 0.13181135144430556 2304 44-45 0.2632499000135624 2304 46-47 0.14879156880098066 2304 48-49 0.14922922280307205 2304 50-51 0.05866058139455532 2304 52-53 0.11426182867777612 2304 54-55 0.380607087851331 2304 56-57 0.3966347027835937 2304 58-59 0.4424857134256328 2304 60-61 0.37792319171785493 2304 62-63 0.4481436578309328 2304 64-65 0.43446766141279625 2304 66-67 0.3641139183134996 2304 68-69 0.29733829966134806 2304 70-71 0.34199130004439837 2304 72-73 0.19338877703672352 2304 74-75 0.1703722156329519 2304 76-77 0.28840847611126463 2304 78-79 0.35265940305353993 2304 80-81 0.20886268652101236 2304 82-83 0.26855915309537437 2304 84-85 0.23834532542035802 2304 86-87 0.22430825980586988 2304 88-89 0.36133617558322584 2304 90-91 0.2579144212024822 2304 92-93 0.3375644021760742 2304 94-95 0.3337892375377969 2304 96-97 0.2949407724532733 2304 98-99 0.3836291430108396 2304 100-101 0.324322471220718 2202 1 -0.2624590784155245 2202 2 -0.41796317099746716 2202 3 -0.3744812732191569 2202 4 -0.3537651626973286 2202 5 -0.3836664137137049 2202 6 -0.45264974445623096 2202 7 -0.06169230806134607 2202 8 -0.48422800418898504 2202 9 -0.479384138445333 2202 10-11 -0.4279375231213791 2202 12-13 -0.49291685875144964 2202 14-15 -0.3334866440634201 2202 16-17 -0.27203139801569165 2202 18-19 -0.4022073864133091 2202 20-21 -0.2870967051712654 2202 22-23 -0.22575645578269388 2202 24-25 -0.2837312494024573 2202 26-27 -0.33521294245539934 2202 28-29 -0.46092806533101793 2202 30-31 -0.17857456707324815 2202 32-33 -0.21036429708365034 2202 34-35 -0.13002727166824712 2202 36-37 -0.04145847221862908 2202 38-39 -0.20321924368963096 2202 40-41 -0.20186641600290045 2202 42-43 -0.08356526436173084 2202 44-45 -0.17907841705795136 2202 46-47 -0.19833195688212868 2202 48-49 -0.09551898311457307 2202 50-51 -0.26437710565409134 2202 52-53 -0.13670864536745597 2202 54-55 -0.11180187803281427 2202 56-57 0.1653810240658231 2202 58-59 0.15354691031061662 2202 60-61 -0.02862336952996003 2202 62-63 0.02011055503058401 2202 64-65 0.0436493164962215 2202 66-67 0.033825478542603094 2202 68-69 0.02527717069116875 2202 70-71 -0.05029357985961269 2202 72-73 -0.04540528268301358 2202 74-75 0.08292272725771 2202 76-77 0.13958948600419063 2202 78-79 0.20700227086871692 2202 80-81 0.11572731536668357 2202 82-83 -0.011506299783082596 2202 84-85 -0.08465529147231621 2202 86-87 -0.05132080093259006 2202 88-89 -0.06252625169135584 2202 90-91 -0.06788796373854566 2202 92-93 -0.0817243884879062 2202 94-95 -0.06039952030070239 2202 96-97 -0.0730901797120751 2202 98-99 -0.17698141094745878 2202 100-101 0.007003067965335674 1102 1 -0.10099297172470045 1102 2 -0.29643011294637844 1102 3 -0.3801442969057156 1102 4 -0.1707808992733959 1102 5 -0.22088872961380446 1102 6 -0.19876043510617336 1102 7 -0.0023194444070995246 1102 8 -0.35661272484809814 1102 9 -0.08877850444000046 1102 10-11 -0.02843475657405037 1102 12-13 -0.16294521046960853 1102 14-15 -0.22631368551130038 1102 16-17 -0.09896213172260815 1102 18-19 -0.12328693461579832 1102 20-21 -0.1950571460934185 1102 22-23 -0.22376346985921458 1102 24-25 -0.32181914887868857 1102 26-27 -0.2546451107409453 1102 28-29 -0.23874993706726855 1102 30-31 0.023158756848729922 1102 32-33 0.026858607026376546 1102 34-35 -0.06203183879149776 1102 36-37 0.008834521678366514 1102 38-39 -0.10137592451254562 1102 40-41 -0.05511902079249609 1102 42-43 0.04924835282864848 1102 44-45 0.00424962238609794 1102 46-47 0.02676166653019152 1102 48-49 0.06234472995401319 1102 50-51 0.2262843429370136 1102 52-53 0.18307873635333038 1102 54-55 0.19542624153849886 1102 56-57 0.2997330062678998 1102 58-59 0.304837704302507 1102 60-61 0.16753529290681968 1102 62-63 0.24902303331583653 1102 64-65 0.19265909777362822 1102 66-67 0.13844292641740452 1102 68-69 0.14517637765348468 1102 70-71 0.25749529983577446 1102 72-73 0.2297433056785323 1102 74-75 0.3898111229216852 1102 76-77 0.20528044312629135 1102 78-79 0.3596689532209787 1102 80-81 0.3684453026953385 1102 82-83 0.2947962388002203 1102 84-85 0.1852301252351971 1102 86-87 0.11622628527153367 1102 88-89 0.10286256160166829 1102 90-91 0.03289389795892461 1102 92-93 0.06748211632953627 1102 94-95 0.02281224964908901 1102 96-97 0.1803577107710801 1102 98-99 0.11302549071560719 1102 100-101 -0.04176058013873174 2305 1 -0.2529093863935046 2305 2 -0.47637949454294315 2305 3 -0.39846936252089904 2305 4 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-1.2067183349103203 1213 60-61 -0.574860426646822 1213 62-63 -0.6316372002107755 1213 64-65 -0.40982365941000864 1213 66-67 -0.26544744997473657 1213 68-69 -0.06577511118324253 1213 70-71 -0.2644340266075922 1213 72-73 0.18846592558451647 1213 74-75 -0.13129990441630213 1213 76-77 -0.3607630309920218 1213 78-79 -0.46823106969998207 1213 80-81 -0.3639548642570851 1213 82-83 -0.5530271682921715 1213 84-85 -0.5167773360874612 1213 86-87 -0.43009024736077706 1213 88-89 -0.3846524718309716 1213 90-91 -0.2865478357885465 1213 92-93 -0.25076171469412856 1213 94-95 -0.36819780932983903 1213 96-97 -0.32578728683581915 1213 98-99 -0.3303248130388674 1213 100-101 -0.1249786326329918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3316.0 3 427.0 4 440.0 5 318.0 6 679.0 7 3995.0 8 24041.0 9 75513.0 10 92664.0 11 53349.0 12 27802.0 13 17113.0 14 12519.0 15 11148.0 16 10291.0 17 9463.0 18 9383.0 19 9821.0 20 10454.0 21 11407.0 22 12202.0 23 13337.0 24 14977.0 25 17322.0 26 20035.0 27 24045.0 28 29703.0 29 37126.0 30 44041.0 31 56943.0 32 77649.0 33 110819.0 34 167461.0 35 282408.0 36 551475.0 37 943175.0 38 260606.0 39 369.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.393626991565135 22.92507275686874 23.527779147963628 13.153521103602491 2 31.34527266053694 25.22647710035639 31.755075836610224 11.673174402496445 3 36.45058060578718 19.92463995338581 29.043573877376005 14.581205563451002 4 27.39217911187818 17.6938806444708 36.05438038849616 18.859559855154856 5 33.316672377428524 16.189878337753967 33.4462661153644 17.0471831694531 6 30.363690423722232 16.46832575316045 38.18265958160603 14.98532424151128 7 36.70013607428643 18.338492844097274 28.640414654983616 16.320956426632677 8 27.882840494279197 32.34045449434102 24.08394539544506 15.692759615934722 9 32.52311873331665 27.513059819876574 23.063630394850573 16.900191051956202 10-11 27.102372700832266 29.793893650471443 26.790603871304956 16.313129777391335 12-13 29.567530669304194 23.801711345365515 26.849192416365337 19.781565568964957 14-15 25.980367415940737 24.339731922455417 28.961242670332616 20.71865799127123 16-17 27.142640594707924 25.650675517489013 29.81993434222537 17.3867495455777 18-19 26.871533451474072 25.75950906932933 30.23412250909892 17.13483497009768 20-21 25.551804841292515 28.074907184615906 29.208647142423654 17.16464083166792 22-23 28.75465321147591 25.668687070521766 28.326789450869285 17.249870267133037 24-25 25.436166447147045 29.161355907501374 28.152752683736605 17.24972496161498 26-27 22.365735523089604 32.10376023176359 28.057742030328264 17.472762214818555 28-29 23.724509644892304 30.286590520350742 27.816681766689484 18.172218068067476 30-31 26.790599922318847 26.976030756469587 28.060390512366446 18.17297880884512 32-33 23.30919970427111 29.037744951944056 30.06061124988268 17.592444093902156 34-35 27.359172833879143 27.02408176814794 28.48982113546966 17.12692426250326 36-37 24.74228382965241 27.37031360023397 29.694715846926144 18.192686723187474 38-39 20.933873911494214 29.8220617012602 31.358764130568662 17.885300256676924 40-41 21.601916926699225 31.188674705897952 28.944970693950584 18.264437673452242 42-43 24.493884367455966 27.625464546202867 30.251143282887632 17.629507803453542 44-45 22.450243317837867 29.015073700214707 30.985693744453112 17.548989237494318 46-47 23.922552913506262 29.969789594515778 28.425881559595506 17.681775932382454 48-49 21.083056656798714 30.038399524045534 29.835654385506317 19.042889433649435 50-51 19.811012887042466 27.56564230694023 31.950060301191314 20.67328450482599 52-53 24.974794501049736 26.889572237971542 28.186765828064893 19.948867432913836 54-55 27.437516098156102 26.876970891898782 27.39942399068267 18.28608901926245 56-57 28.095705553807814 26.825242574526502 26.77915712043325 18.299894751232433 58-59 26.37863394704044 26.668448774208127 27.507867543621696 19.44504973512974 60-61 22.1997166637444 26.90362533760396 27.33187302386956 23.564784974782086 62-63 24.509061082405854 26.94361188588612 27.300012046773077 21.247314984934953 64-65 22.189357294587253 28.245594512054595 29.600855746526243 19.96419244683191 66-67 20.47879565162274 28.19354328867845 28.824528851710323 22.50313220798849 68-69 20.086323436159375 27.255787874617504 33.50122378508793 19.15666490413519 70-71 19.342171828965597 31.465441622514234 30.84565635194324 18.346730196576928 72-73 19.21742367196057 35.57335219373999 26.776226409133034 18.432997725166402 74-75 19.896099894984072 35.324538064036844 26.186786161991144 18.592575878987944 76-77 20.143143545628693 34.757060688867206 26.456555361143174 18.64324040436093 78-79 19.97093116562569 34.60369845775514 27.00338340821384 18.42198696840534 80-81 19.203395675731734 34.81305099686656 26.945826066272303 19.037727261129405 82-83 18.81455070159708 33.977809952855864 27.236580451433834 19.971058894113224 84-85 18.96710737164971 32.97788943167733 27.82032688739575 20.2346763092772 86-87 19.206597686856917 32.70071189469393 28.409020352200532 19.683670066248617 88-89 19.640720357642675 32.99884753580494 27.80200474094588 19.558427365606505 90-91 20.07836855116141 32.29388535965156 27.41934501343049 20.20840107575654 92-93 19.674982041660712 32.77522019129664 27.53164848666013 20.018149280382513 94-95 19.350391105913666 33.09961824982099 27.624596251090473 19.925394393174873 96-97 19.956932173687477 33.456093481839375 26.87769122478637 19.70928311968678 98-99 20.6303163534919 32.359387471780025 26.514279900001842 20.49601627472623 100-101 20.50080548523672 32.3861382437606 26.083494147696502 21.02956212330618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2886.0 1 1847.0 2 633.5 3 478.5 4 477.0 5 456.0 6 534.0 7 678.5 8 843.0 9 1055.0 10 1259.5 11 1326.5 12 1255.0 13 1155.5 14 1082.0 15 1013.5 16 956.5 17 992.5 18 1044.0 19 1110.5 20 1204.0 21 1333.5 22 1515.0 23 1772.5 24 2252.5 25 3058.0 26 4628.5 27 6984.0 28 9280.0 29 11371.0 30 13807.5 31 16080.0 32 19424.0 33 28302.5 34 44962.5 35 74586.0 36 112578.5 37 152156.0 38 196510.5 39 226587.5 40 230535.0 41 209960.0 42 184696.0 43 178938.5 44 172877.5 45 160042.0 46 157304.5 47 145712.0 48 118859.0 49 98292.5 50 83112.0 51 72192.5 52 62943.0 53 51568.0 54 41091.0 55 32245.0 56 24571.0 57 18171.5 58 13732.0 59 10502.5 60 8047.0 61 6116.0 62 4580.0 63 3376.0 64 2493.5 65 1863.5 66 1416.0 67 1100.0 68 815.5 69 588.5 70 436.5 71 327.0 72 250.5 73 198.5 74 156.5 75 124.0 76 90.5 77 69.0 78 65.5 79 58.0 80 45.5 81 36.0 82 33.5 83 32.0 84 26.0 85 17.5 86 14.0 87 14.5 88 9.5 89 6.0 90 8.0 91 7.0 92 6.0 93 8.0 94 8.0 95 4.5 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2259307915517764 2 0.2412203281278914 3 0.21989372131571383 4 0.2138894612439777 5 0.22291225643374513 6 0.2443701039032284 7 0.22474962563602502 8 0.2125442445065942 9 0.20522757786180096 10-11 0.19156214441984412 12-13 0.21664551504739757 14-15 0.21374181550450877 16-17 0.2169408065263354 18-19 0.20558848966939167 20-21 0.1983374433532513 22-23 0.16552727902682426 24-25 0.4946952526317033 26-27 0.3478861723531056 28-29 0.48322810019961704 30-31 0.3207521664551505 32-33 0.36980336212315884 34-35 0.3723297447762937 36-37 0.378957397970232 38-39 0.3718704024757238 40-41 0.3778910676296231 42-43 0.43158490154982093 44-45 0.386717001833432 46-47 0.40331894498260407 48-49 0.40197372824522054 50-51 0.415229034633097 52-53 0.41944514074904293 54-55 0.38630687477935166 56-57 0.39933251001694314 58-59 0.39715063408923573 60-61 0.35462866112218633 62-63 0.3175367703511606 64-65 0.3441130034555665 66-67 0.35569499146279526 68-69 0.3457535116718879 70-71 0.3178320618300985 72-73 0.307496860067274 74-75 0.3190952531566659 76-77 0.3250338928997492 78-79 0.33527066416959445 80-81 0.3426529511430405 82-83 0.3602227941398422 84-85 0.3893582200617094 86-87 0.39121199434615245 88-89 0.38496165804196814 90-91 0.35774562673319693 92-93 0.3363041843458769 94-95 0.3147315013012511 96-97 0.3303819496849568 98-99 0.3602556043041686 100-101 0.37625055941330177 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3047836.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.38626823724175 #Duplication Level Percentage of deduplicated Percentage of total 1 89.7670677196565 44.33260485273601 2 4.509536000160954 4.454183090588942 3 1.501706855800687 2.2249109278283297 4 0.9157708182734451 1.8090601310036294 5 0.6263376904728151 1.546624059439247 6 0.38084362047519305 1.1285067118338108 7 0.2852572267373598 0.9861452941384059 8 0.24154594744936647 0.9543242361882491 9 0.17252186786778254 0.7668190118977458 >10 1.2969024255225765 12.551893754808951 >50 0.15231771064027008 5.309555454365888 >100 0.12783155995531825 13.587434276779875 >500 0.017759885339081575 5.974724326060515 >1k 0.004335195868459229 3.502915120833098 >5k 1.9910683898473298E-4 0.5407901292347583 >10k+ 6.63689412792904E-5 0.32950862226249794 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 10053 0.32984058197357075 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5651 0.18541023860863906 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5491 0.18016061231641073 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5357 0.1757640502966695 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4720 0.1548639756207355 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4385 0.14387257057138245 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3852 0.12638475298539684 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3319 0.10889693539941127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004823094155984771 0.0 0.0 2 0.005282436456554749 0.0 0.0 3 0.00603707023606257 0.0 0.0 4 0.006397982043653268 0.0 0.0 5 0.007480717466425359 0.0 0.0 6 0.008497832560544595 0.0 0.0 7 0.009908669626580957 0.0 0.0 8 0.011614798171555163 0.0 0.0 9 0.013550597866814356 0.0 0.0 10-11 0.022179671084664662 0.0 0.0 12-13 0.03144854250688029 0.0 0.0 14-15 0.04301412543194581 0.0 0.0 16-17 0.0552687218078663 0.0 0.0 18-19 0.06760534359460285 0.0 0.0 20-21 0.07674297435951277 0.0 0.0 22-23 0.08632354234282946 0.0 0.0 24-25 0.09780709985707892 0.0 0.0 26-27 0.11037339279410047 0.0 0.0 28-29 0.12451457361879051 0.0 0.0 30-31 0.22269899036562335 0.0 3.281016432642701E-5 32-33 0.23949779450075398 0.0 3.281016432642701E-5 34-35 0.25224454334157087 0.0 3.281016432642701E-5 36-37 0.265549064975937 0.0 3.281016432642701E-5 38-39 0.2808221964698888 0.0 3.281016432642701E-5 40-41 0.29604611271735093 1.6405082163213505E-5 3.281016432642701E-5 42-43 0.3128121066881552 3.281016432642701E-5 3.281016432642701E-5 44-45 0.33200605281911494 3.281016432642701E-5 3.281016432642701E-5 46-47 0.3516593412506447 3.281016432642701E-5 3.281016432642701E-5 48-49 0.37214928887249843 3.281016432642701E-5 3.281016432642701E-5 50-51 0.39372197191712416 3.281016432642701E-5 3.281016432642701E-5 52-53 0.41750934105378373 3.281016432642701E-5 3.281016432642701E-5 54-55 0.44467615711606534 3.281016432642701E-5 3.281016432642701E-5 56-57 0.4735983169698107 3.281016432642701E-5 3.281016432642701E-5 58-59 0.5054897966950977 3.281016432642701E-5 3.281016432642701E-5 60-61 0.5397436082518876 3.281016432642701E-5 3.281016432642701E-5 62-63 0.5767698786942604 3.281016432642701E-5 6.562032865285402E-5 64-65 0.6153218217778123 3.281016432642701E-5 6.562032865285402E-5 66-67 0.6562525017750299 3.281016432642701E-5 6.562032865285402E-5 68-69 0.7033678977477791 3.281016432642701E-5 6.562032865285402E-5 70-71 0.7543220829467202 3.281016432642701E-5 6.562032865285402E-5 72-73 0.8067199153760242 3.281016432642701E-5 6.562032865285402E-5 74-75 0.8640556775364554 3.281016432642701E-5 6.562032865285402E-5 76-77 0.9254434949912003 3.281016432642701E-5 6.562032865285402E-5 78-79 0.9936230164615156 3.281016432642701E-5 6.562032865285402E-5 80-81 1.0709237636145779 3.281016432642701E-5 6.562032865285402E-5 82-83 1.1545240623183137 3.281016432642701E-5 6.562032865285402E-5 84-85 1.2411428961400812 3.281016432642701E-5 6.562032865285402E-5 86-87 1.3364400184261882 4.9215246489640516E-5 6.562032865285402E-5 88-89 1.4404318342587987 6.562032865285402E-5 6.562032865285402E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 3700 0.0 86.51254 4 GAGCGGC 2895 0.0 86.30562 9 AAGCGGC 1150 0.0 84.927124 9 AGAGCGG 3045 0.0 84.54949 8 GTTCGGA 745 0.0 84.025085 2 CGGAAAG 1845 0.0 82.50603 5 TCGGAGA 1160 0.0 81.76153 4 GAGAGCG 3400 0.0 80.465485 7 ATTCGGA 810 0.0 79.62422 2 TCGGGAG 4155 0.0 77.15297 3 AAAGCGG 1375 0.0 75.86098 8 GGATCGG 1790 0.0 74.97888 1 AAGGGGC 3005 0.0 74.942154 9 GATCCGG 920 0.0 74.74562 1 TCGGGAA 1525 0.0 73.38701 3 TTCGGAG 810 0.0 73.18162 3 ACCGGAA 430 0.0 72.78678 3 CGGAGAG 1950 0.0 72.47007 5 ATCGGGA 5515 0.0 71.11337 2 ATCCGGA 950 0.0 70.386086 2 >>END_MODULE