##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_40825-1.342000000356f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1809825 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.480250852983023 33.0 33.0 33.0 27.0 33.0 2 31.215474976862403 33.0 33.0 33.0 27.0 33.0 3 31.153644136864063 33.0 33.0 33.0 27.0 33.0 4 34.733548271241695 37.0 37.0 37.0 33.0 37.0 5 34.74442335584932 37.0 37.0 37.0 33.0 37.0 6 34.99310706836296 37.0 37.0 37.0 33.0 37.0 7 34.76725595016093 37.0 37.0 37.0 33.0 37.0 8 34.74447584711229 37.0 37.0 37.0 33.0 37.0 9 34.49748953628113 37.0 37.0 37.0 33.0 37.0 10-11 34.46371168483141 37.0 37.0 37.0 27.0 37.0 12-13 34.47856091058527 37.0 37.0 37.0 27.0 37.0 14-15 36.08385313497161 40.0 37.0 40.0 27.0 40.0 16-17 35.94361250397138 40.0 37.0 40.0 27.0 40.0 18-19 35.88378904037683 40.0 37.0 40.0 27.0 40.0 20-21 35.79162570966806 40.0 37.0 40.0 27.0 40.0 22-23 36.128686198941885 40.0 37.0 40.0 27.0 40.0 24-25 35.66537648667689 37.0 37.0 40.0 27.0 40.0 26-27 35.44394817178457 37.0 37.0 40.0 27.0 40.0 28-29 35.20752973353777 37.0 37.0 40.0 22.0 40.0 30-31 35.22207423369663 37.0 37.0 40.0 24.5 40.0 32-33 35.0546610860166 37.0 37.0 40.0 22.0 40.0 34-35 34.90596908540881 37.0 37.0 40.0 22.0 40.0 36-37 34.76329120218806 37.0 37.0 40.0 22.0 40.0 38-39 34.64402552732999 37.0 35.0 40.0 22.0 40.0 40-41 34.51906703686821 37.0 33.0 40.0 22.0 40.0 42-43 34.28200488997555 37.0 33.0 40.0 22.0 40.0 44-45 34.10248891467407 37.0 33.0 40.0 22.0 40.0 46-47 33.91675189590154 37.0 33.0 40.0 22.0 40.0 48-49 33.71490641360352 37.0 33.0 40.0 15.0 40.0 50-51 32.5118776677303 35.0 33.0 38.5 15.0 38.5 52-53 33.000623264680286 37.0 33.0 37.0 18.5 40.0 54-55 33.65622173414557 37.0 33.0 40.0 22.0 40.0 56-57 33.693236915171354 37.0 33.0 40.0 22.0 40.0 58-59 33.372448993687236 37.0 33.0 38.5 18.5 40.0 60-61 33.08898374152197 37.0 33.0 37.0 15.0 40.0 62-63 32.80171011009352 37.0 33.0 37.0 15.0 40.0 64-65 32.52937853107345 37.0 33.0 37.0 15.0 40.0 66-67 32.32080698410067 37.0 33.0 37.0 15.0 38.5 68-69 32.20344149296203 37.0 33.0 37.0 15.0 37.0 70-71 31.934402497479038 37.0 33.0 37.0 15.0 37.0 72-73 31.85276449380465 37.0 33.0 37.0 15.0 37.0 74-75 31.75464893014518 35.0 33.0 37.0 15.0 37.0 76-77 31.548320417719946 33.0 33.0 37.0 15.0 37.0 78-79 31.30692166369677 33.0 33.0 37.0 15.0 37.0 80-81 31.046065227300982 33.0 33.0 37.0 15.0 37.0 82-83 30.77382868509386 33.0 27.0 37.0 15.0 37.0 84-85 30.62827621455113 33.0 27.0 37.0 15.0 37.0 86-87 30.417997596452693 33.0 27.0 37.0 15.0 37.0 88-89 30.28282568756648 33.0 27.0 37.0 15.0 37.0 90-91 30.168024809028495 33.0 27.0 37.0 15.0 37.0 92-93 29.97925932065255 33.0 27.0 37.0 15.0 37.0 94-95 29.80655892144268 33.0 27.0 37.0 15.0 37.0 96-97 29.61050709322725 33.0 27.0 37.0 15.0 37.0 98-99 29.454877681543795 33.0 27.0 37.0 15.0 37.0 100-101 28.043785725139173 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.012295700409556076 2306 2 0.10901936781269228 2306 3 0.09240362341861896 2306 4 0.22587568675260883 2306 5 0.262910395525509 2306 6 0.04517230241529546 2306 7 0.23157651731969509 2306 8 0.0378457190858299 2306 9 0.20730646324259538 2306 10-11 0.15734112061171857 2306 12-13 0.1772901099538231 2306 14-15 0.36502681660235936 2306 16-17 0.4900753627508365 2306 18-19 0.4351167636027 2306 20-21 0.45957881107991483 2306 22-23 0.46148255916778425 2306 24-25 0.29544471693228047 2306 26-27 0.28277511905218944 2306 28-29 0.38555146131530904 2306 30-31 0.46379440563270435 2306 32-33 0.538771114525531 2306 34-35 0.5760635611997245 2306 36-37 0.532382685648777 2306 38-39 0.4029261408043894 2306 40-41 0.6738186656937657 2306 42-43 0.6574931320059036 2306 44-45 0.7367440031794317 2306 46-47 0.5730486925181921 2306 48-49 0.7096123556232783 2306 50-51 0.5103933278751356 2306 52-53 0.7660399594751794 2306 54-55 0.8497259685773599 2306 56-57 0.8504737043101684 2306 58-59 1.005377762750534 2306 60-61 0.9492136912394145 2306 62-63 1.010531359441785 2306 64-65 0.9827860941453821 2306 66-67 0.7615155746832869 2306 68-69 0.7194512875598207 2306 70-71 0.7804210634055764 2306 72-73 0.7042593516495401 2306 74-75 0.8203785603055387 2306 76-77 0.708459126103893 2306 78-79 0.6646464868476194 2306 80-81 0.6969980709530823 2306 82-83 0.4612080526308837 2306 84-85 0.5640448993052125 2306 86-87 0.7011165826014647 2306 88-89 0.7773794294655367 2306 90-91 0.7449828650346255 2306 92-93 0.6034876532700686 2306 94-95 0.5779424981516641 2306 96-97 0.6021096334388574 2306 98-99 0.6706288037364132 2306 100-101 0.4730380659486322 2307 1 -0.01813049009218659 2307 2 -0.0027630771063336113 2307 3 -0.06351923230355183 2307 4 0.038153992006947135 2307 5 0.18850477124383502 2307 6 -0.06342790782580465 2307 7 0.11480582883374524 2307 8 0.10820903145126692 2307 9 0.20199013317121484 2307 10-11 0.09540866043820273 2307 12-13 0.05366818825611119 2307 14-15 0.3099047065870906 2307 16-17 0.37127815240598494 2307 18-19 0.32183723236779116 2307 20-21 0.3417099015689331 2307 22-23 0.1724414149737683 2307 24-25 0.37276888019670906 2307 26-27 0.27232081123039364 2307 28-29 0.28277631964275685 2307 30-31 0.5807316925617343 2307 32-33 0.5534391875692393 2307 34-35 0.569300993110275 2307 36-37 0.6573555367460457 2307 38-39 0.5659445059830688 2307 40-41 0.40413973564415784 2307 42-43 0.6318671230249251 2307 44-45 0.5228114162684463 2307 46-47 0.5242987174313001 2307 48-49 0.4691844838059609 2307 50-51 0.36275695606215663 2307 52-53 0.5430395112947082 2307 54-55 0.5252453494443898 2307 56-57 0.756722219105491 2307 58-59 0.8117073281994465 2307 60-61 0.8181017386187861 2307 62-63 0.9698430114697629 2307 64-65 0.9472004622901196 2307 66-67 0.7245447738711519 2307 68-69 0.5496080076350864 2307 70-71 0.4297239306874374 2307 72-73 0.4131707330317056 2307 74-75 0.4165086726675007 2307 76-77 0.24684422504854453 2307 78-79 0.16474230650429433 2307 80-81 0.3869036873136622 2307 82-83 0.536744859899482 2307 84-85 0.6590949324971405 2307 86-87 0.44788771569061936 2307 88-89 0.5213278432286081 2307 90-91 0.5502494710921653 2307 92-93 0.27667184522418253 2307 94-95 0.235519839945745 2307 96-97 0.48128998954047475 2307 98-99 0.38473258843399094 2307 100-101 0.16630937968978188 2201 1 0.11715478027970505 2201 2 0.18649015444438533 2201 3 0.13520139050010727 2201 4 0.17081098175773946 2201 5 0.15474671305937449 2201 6 0.14744561592426209 2201 7 0.3171564775087248 2201 8 0.16670234605508227 2201 9 0.24773649959809063 2201 10-11 0.20097501541926022 2201 12-13 0.1026245853785781 2201 14-15 0.30772432622659807 2201 16-17 0.4427745271097976 2201 18-19 0.502179755460439 2201 20-21 0.46072958174828926 2201 22-23 0.17888890848228556 2201 24-25 0.3512710865902804 2201 26-27 0.20536492491287106 2201 28-29 0.24925096148808734 2201 30-31 0.3253996929968608 2201 32-33 0.4367216768919917 2201 34-35 0.43498696158225414 2201 36-37 0.48374759845885507 2201 38-39 0.36964358511526285 2201 40-41 0.1831331188282661 2201 42-43 0.4036118056873548 2201 44-45 0.4438780999619496 2201 46-47 0.3657909773175092 2201 48-49 0.4147456834408345 2201 50-51 0.485576930427321 2201 52-53 0.5726326173198402 2201 54-55 0.5122659377214234 2201 56-57 0.6553444646607787 2201 58-59 0.7865130567822902 2201 60-61 0.6530507290695695 2201 62-63 0.5921122151343141 2201 64-65 0.6901844617982391 2201 66-67 0.5720401742618151 2201 68-69 0.6527340865922824 2201 70-71 0.6192221594239911 2201 72-73 0.5462630894032827 2201 74-75 0.34191979970185216 2201 76-77 0.5033919876755952 2201 78-79 0.5625926690339149 2201 80-81 0.5516011536265495 2201 82-83 0.6000205857864138 2201 84-85 0.6272199945741299 2201 86-87 0.7059936698789855 2201 88-89 0.7916004144220459 2201 90-91 0.6031747753285366 2201 92-93 0.29736464807712437 2201 94-95 0.46523024192086027 2201 96-97 0.6636837728551264 2201 98-99 0.5536283511177942 2201 100-101 0.23026073970794414 1101 1 0.13476411653819298 1101 2 0.13296331097037495 1101 3 0.11278112905371174 1101 4 0.22740923618550113 1101 5 0.1448570121086945 1101 6 0.06687062425402246 1101 7 0.3775470460529462 1101 8 0.06971923492089616 1101 9 0.15164002146534017 1101 10-11 0.20554015849646845 1101 12-13 0.17480533908153717 1101 14-15 0.34939910629920234 1101 16-17 0.2811037712865314 1101 18-19 0.34688483856334784 1101 20-21 0.35255061948535626 1101 22-23 0.19878600885918019 1101 24-25 -0.00519432433750211 1101 26-27 0.16356876828735523 1101 28-29 0.2548273594820145 1101 30-31 0.32189643248946 1101 32-33 0.44899044482261274 1101 34-35 0.4708648753767761 1101 36-37 0.4821872282279074 1101 38-39 0.379418183880297 1101 40-41 0.4585382482316831 1101 42-43 0.5044549508329368 1101 44-45 0.42055930585205914 1101 46-47 0.3682403604822966 1101 48-49 0.3879690638968327 1101 50-51 0.4579543530903294 1101 52-53 0.5920026708466821 1101 54-55 0.7764936063828998 1101 56-57 0.6725604629991508 1101 58-59 0.7077833781597107 1101 60-61 0.8047869979413491 1101 62-63 0.673994871485057 1101 64-65 0.7004954107854644 1101 66-67 0.6455034951762357 1101 68-69 0.6435758903214222 1101 70-71 0.8299383459933907 1101 72-73 0.5267058866460275 1101 74-75 0.5630008082739373 1101 76-77 0.4994419489476094 1101 78-79 0.512702362282262 1101 80-81 0.49261193255679103 1101 82-83 0.5299075606326902 1101 84-85 0.4155386372337695 1101 86-87 0.5801129867085706 1101 88-89 0.5185464418821404 1101 90-91 0.6330842513539636 1101 92-93 0.5114940904373242 1101 94-95 0.5281292592251319 1101 96-97 0.5732861510566742 1101 98-99 0.6667262009570436 1101 100-101 0.3375342458676407 2304 1 0.06872906149604674 2304 2 0.07193219603854928 2304 3 -0.039576967276012454 2304 4 0.09607819527998629 2304 5 0.07244494820726999 2304 6 0.007086016951689089 2304 7 0.25487708402128106 2304 8 0.10898422524971352 2304 9 0.2023516292691454 2304 10-11 0.1567377132401262 2304 12-13 0.12158638834485913 2304 14-15 0.09876749467468215 2304 16-17 0.10768877021195777 2304 18-19 0.20742045477272342 2304 20-21 0.147623690622261 2304 22-23 0.09879735160102143 2304 24-25 0.06290212478732116 2304 26-27 -0.10414083364210569 2304 28-29 -0.13061273890134117 2304 30-31 0.11492243721759365 2304 32-33 0.3151395242695685 2304 34-35 0.35313676686853057 2304 36-37 0.253757541287591 2304 38-39 0.06393474428337953 2304 40-41 0.19461294317162725 2304 42-43 0.2712499695783066 2304 44-45 0.25936819856166693 2304 46-47 0.25876678310091705 2304 48-49 0.3987978004347852 2304 50-51 0.17361032176048496 2304 52-53 0.2456854800259478 2304 54-55 0.44988711594662334 2304 56-57 0.6619854456865468 2304 58-59 0.630752430384085 2304 60-61 0.4767208757262935 2304 62-63 0.44951962254172173 2304 64-65 0.40865740901838876 2304 66-67 0.5041430825392226 2304 68-69 0.5175634013656705 2304 70-71 0.5991625024515841 2304 72-73 0.4258844333423042 2304 74-75 0.5557486173338013 2304 76-77 0.6346766840050329 2304 78-79 0.5382617385508581 2304 80-81 0.3251035150483723 2304 82-83 0.3564627102374871 2304 84-85 0.4603275428382787 2304 86-87 0.36766820795173416 2304 88-89 0.5556778027723688 2304 90-91 0.3665996655431343 2304 92-93 0.4759005665459277 2304 94-95 0.41321474767941524 2304 96-97 0.412329062440012 2304 98-99 0.2516087140310468 2304 100-101 0.324245328323407 2202 1 -0.10681699738334771 2202 2 -0.21472166115794167 2202 3 -0.158548234309297 2202 4 -0.18307439201213072 2202 5 -0.2578008611083078 2202 6 -0.2202555762813816 2202 7 -0.0851182780671067 2202 8 -0.1613198892404455 2202 9 -0.20566422797151773 2202 10-11 -0.1682385450694639 2202 12-13 -0.3647196254805394 2202 14-15 -0.24593093064390104 2202 16-17 -0.15512180422652477 2202 18-19 -0.44652618715841896 2202 20-21 -0.2281117496480647 2202 22-23 -0.12725501262963945 2202 24-25 -0.37328738911536874 2202 26-27 -0.1755839971395261 2202 28-29 -0.3847034873719366 2202 30-31 -0.40200262738188997 2202 32-33 -0.26344160605574984 2202 34-35 -0.19486120128448192 2202 36-37 -0.1964147240009524 2202 38-39 -0.262916555436135 2202 40-41 -0.28624352450654555 2202 42-43 -0.3736080219851132 2202 44-45 -0.16049752285444896 2202 46-47 -0.24756120559141692 2202 48-49 -0.2602566177281602 2202 50-51 -0.12079050422948967 2202 52-53 -0.049604279023498066 2202 54-55 0.09059472874726282 2202 56-57 0.1680357118143334 2202 58-59 0.2973147440394186 2202 60-61 0.19634752384131815 2202 62-63 0.26176039641040205 2202 64-65 0.24784555363288518 2202 66-67 0.09970062577119165 2202 68-69 0.22375556550356634 2202 70-71 0.1698442754589351 2202 72-73 -0.0136195309678655 2202 74-75 -0.008113217070661705 2202 76-77 -0.02696723480942609 2202 78-79 0.041741276526519044 2202 80-81 0.03061269148778578 2202 82-83 -0.06397612454243529 2202 84-85 0.04026802297255827 2202 86-87 0.059586094830862635 2202 88-89 0.11085459554606203 2202 90-91 0.03558584014946575 2202 92-93 0.1720477871117545 2202 94-95 0.02469965238137206 2202 96-97 -0.05844546879963275 2202 98-99 0.1260455390492652 2202 100-101 -0.14875540049127522 1102 1 -0.47369573268313303 1102 2 -0.6266190253196875 1102 3 -0.6100123149043455 1102 4 -0.6054137825277763 1102 5 -0.6194428910489904 1102 6 -0.5032125214170264 1102 7 -0.4645507590907769 1102 8 -0.6527855838885159 1102 9 -0.5730630470227212 1102 10-11 -0.4806541995903828 1102 12-13 -0.509838684308292 1102 14-15 -0.5752044722797436 1102 16-17 -0.5107290692585096 1102 18-19 -0.4631666254634794 1102 20-21 -0.6378704935561359 1102 22-23 -0.5418760912654008 1102 24-25 -0.575824142447722 1102 26-27 -0.6038953838352512 1102 28-29 -0.5829024732086125 1102 30-31 -0.41840768295462993 1102 32-33 -0.438963146938363 1102 34-35 -0.3352815724270073 1102 36-37 -0.2880660347465138 1102 38-39 -0.47911447420442954 1102 40-41 -0.45972732344934286 1102 42-43 -0.48850393798262104 1102 44-45 -0.3695980918081361 1102 46-47 -0.4476522202479103 1102 48-49 -0.5138333143893163 1102 50-51 -0.27638521551575224 1102 52-53 -0.1344541496750793 1102 54-55 -0.16781740175788684 1102 56-57 -0.017826977108327924 1102 58-59 -0.0018724009249595497 1102 60-61 -0.04900422763039103 1102 62-63 0.06261539616381384 1102 64-65 0.09045520836004783 1102 66-67 -0.11119333542877285 1102 68-69 -0.1790111903843581 1102 70-71 -0.19657041959712274 1102 72-73 -0.15265450660015034 1102 74-75 -0.1737217823120325 1102 76-77 -0.24810594921619256 1102 78-79 -0.03817900718813405 1102 80-81 -0.1997395755841893 1102 82-83 0.0018436126359340221 1102 84-85 -0.15592908876923062 1102 86-87 -0.3414096862138791 1102 88-89 -0.1163166420290942 1102 90-91 -0.37024761647958826 1102 92-93 -0.056981853178598385 1102 94-95 -0.06454178753325834 1102 96-97 -0.05932871448092314 1102 98-99 -0.3419558597966841 1102 100-101 -0.05655710511060619 2305 1 -0.059661903214976775 2305 2 0.0018335944871452625 2305 3 -0.033539329343096114 2305 4 0.11301936211053487 2305 5 0.09826025466416866 2305 6 0.0853895786254526 2305 7 0.2603752813336371 2305 8 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8805.0 20 9270.0 21 9891.0 22 10551.0 23 11430.0 24 12746.0 25 14518.0 26 16731.0 27 19753.0 28 23419.0 29 28409.0 30 35758.0 31 46956.0 32 64171.0 33 92076.0 34 140182.0 35 237026.0 36 407127.0 37 417632.0 38 60736.0 39 42.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.527250194094435 22.60759919859211 24.44305141924287 12.422099188070584 2 34.80870234174099 21.800771737161853 31.762627960545807 11.62789796055135 3 39.647363644267095 16.637124331974995 27.530687203988297 16.18482481976961 4 32.79088374616903 15.172051018684888 35.04237252942268 16.994692705723406 5 31.449683695709957 14.506306150656531 37.465279623624475 16.578730530009036 6 27.32219825795667 14.70717255268169 42.43990194221326 15.530727247148382 7 38.33768506391921 15.095235062673225 29.27309024557051 17.293989627837046 8 32.78762321251189 28.533531632434972 22.82619408579943 15.85265106925371 9 33.780441689979895 27.40844170548207 20.87532990550875 17.935786699029286 10-11 28.14383290352599 25.596764224676356 27.38756203881746 18.871840832980197 12-13 29.63105496203791 21.8829295330731 26.12463879743946 22.36137670744953 14-15 27.37516285873911 23.605311811155293 26.230735880638733 22.788789449466865 16-17 27.315331238598496 25.02812221667576 27.501769837756335 20.15477670696941 18-19 27.341227394129447 24.965313304188758 27.016119209287286 20.677340092394505 20-21 26.079130069155003 26.25789284143399 27.221323979428718 20.441653109982287 22-23 28.197223133872797 25.582904457851395 25.444708684749262 20.775163723526543 24-25 26.658521065291406 26.645028351356935 25.639793400452643 21.056657182899013 26-27 23.99910842727514 28.32487180176701 26.053800980230985 21.62221879072687 28-29 24.835241828991276 27.572221970846666 25.367414677044557 22.2251215231175 30-31 26.247666566160888 26.000826955650346 26.09535596782633 21.65615051036244 32-33 25.186262426852956 25.347716204020127 27.599335108779588 21.866686260347336 34-35 23.98047104942323 26.24546553306033 26.763884417848338 23.010178999668103 36-37 24.804898284236245 27.703889057111898 26.161709264059468 21.32950339459239 38-39 25.743725620894303 26.92271220427597 24.771278654633818 22.56228352019591 40-41 25.323815735037876 26.02815239248752 24.17110603121364 24.476925841260968 42-43 26.85848551190457 25.627591816940143 25.134662810979886 22.379259860175406 44-45 25.272114257761046 26.35779814987263 25.495046896552488 22.875040695813837 46-47 25.79113406190835 26.67034448939561 25.218325653174105 22.320195795521933 48-49 24.37096176968618 26.702557424519156 25.731343531966765 23.1951372738279 50-51 23.562281464591194 25.81356545763967 25.750430309309525 24.87372276845961 52-53 26.211707996661087 25.541539855419227 24.43614247467312 23.810609673246567 54-55 26.909434172603714 25.34857595432538 25.011085601363714 22.73090427170719 56-57 27.31176294389776 25.109247939293756 24.621223887551448 22.957765229257035 58-59 26.295109992375878 25.080963008426476 25.067012715290048 23.556914283907602 60-61 24.697820537557856 25.276286286486087 24.909983787655996 25.115909388300057 62-63 26.12824496951266 25.08993231901926 25.19487760586186 23.58694510560622 64-65 25.46699911713058 26.1356237119046 25.648119016896487 22.749258154068336 66-67 24.36388415597348 26.046359345362486 25.22542877626121 24.36432772240282 68-69 23.563988662430095 24.604522553863852 28.10359046147236 23.727898322233695 70-71 23.513521228007896 26.50445017349261 26.859222543592082 23.122806054907414 72-73 23.58432455483355 28.38214418618699 25.351218962069378 22.68231229691008 74-75 24.054179059930792 28.483579923840484 25.158106781278207 22.304134234950514 76-77 24.052598525928722 28.045766382372783 24.87577038986896 23.025864701829533 78-79 24.01950403950477 28.225422881324924 24.97984959538431 22.775223483785993 80-81 24.25164630743989 28.727479259866413 24.829423506196193 22.191450926497506 82-83 23.78312514815087 28.386995715234274 25.07720479683058 22.752674339784278 84-85 23.429101980700214 28.42346268539166 25.025243022152015 23.122192311756105 86-87 23.216197800058406 28.632547964778084 25.02272019452979 23.12853404063372 88-89 23.312215342498575 28.691202211371436 25.157677793775413 22.838904652354575 90-91 23.76856867446632 28.058600259544903 24.796158003572415 23.376673062416362 92-93 23.432806655515012 27.882040060036005 24.45203286735674 24.233120417092245 94-95 23.492187357113682 28.414737083630996 23.97372637543583 24.11934918381949 96-97 23.434429605387514 29.007418419831676 23.657514344329314 23.900637630451495 98-99 23.640250072776166 27.78716089771136 24.27532957207613 24.297259457436336 100-101 24.37526099535657 27.992095133466023 23.872153452237228 23.76049041894018 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3476.0 1 2185.0 2 767.5 3 1051.0 4 1230.5 5 1005.0 6 1313.5 7 1805.5 8 2069.5 9 2060.0 10 1789.0 11 1539.0 12 1402.5 13 1279.0 14 1167.0 15 1077.0 16 1035.5 17 1010.0 18 999.0 19 988.0 20 1002.0 21 1065.5 22 1091.0 23 1174.0 24 1321.0 25 1530.5 26 1805.5 27 2115.5 28 2482.0 29 3037.0 30 3882.5 31 4888.5 32 6220.5 33 7904.0 34 10404.5 35 15011.5 36 20809.0 37 28077.0 38 35676.0 39 39957.0 40 45321.5 41 49281.5 42 55500.0 43 67550.5 44 74107.5 45 79551.5 46 91794.0 47 98607.0 48 95664.0 49 93083.0 50 90414.5 51 88031.5 52 86261.0 53 83859.0 54 78194.5 55 68511.5 56 59634.0 57 53479.5 58 48925.0 59 41654.5 60 32055.5 61 23741.5 62 17122.0 63 12894.5 64 10336.5 65 8561.5 66 7797.0 67 7042.0 68 5839.0 69 4422.5 70 3112.0 71 2344.5 72 2047.5 73 1643.0 74 1080.5 75 699.0 76 543.5 77 439.0 78 368.5 79 308.5 80 253.5 81 182.0 82 135.0 83 113.5 84 89.5 85 61.0 86 39.0 87 32.5 88 29.5 89 25.5 90 17.5 91 15.0 92 13.0 93 9.5 94 6.0 95 4.0 96 2.5 97 2.5 98 3.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22118160595637698 2 0.2369289848466012 3 0.21781111433425882 4 0.21107013109002254 5 0.21930297128175377 6 0.24085201607890266 7 0.22267346290387194 8 0.20990979790863756 9 0.2003508626524664 10-11 0.1876148246377412 12-13 0.21430248780959488 14-15 0.21062809940187588 16-17 0.21374994819941154 18-19 0.20206373544403464 20-21 0.19452156976503251 22-23 0.16399375630240492 24-25 0.4891633168952799 26-27 0.3464699625654414 28-29 0.47653778680259146 30-31 0.3103614990399624 32-33 0.3603939607420607 34-35 0.36210683353362894 36-37 0.36815714226513613 38-39 0.36130565109886315 40-41 0.3668310472006962 42-43 0.42205738123851755 44-45 0.37650049037890404 46-47 0.39385023413865977 48-49 0.3940988769632423 50-51 0.4057022087770917 52-53 0.4111723509179065 54-55 0.37630710151533986 56-57 0.3892641553741384 58-59 0.386529084303731 60-61 0.34594504993576725 62-63 0.30674236459326176 64-65 0.3341483292583537 66-67 0.3460003038967856 68-69 0.33635848769908694 70-71 0.30773693589159173 72-73 0.2961336040777423 74-75 0.308620999267885 76-77 0.31527910157059386 78-79 0.325501084358985 80-81 0.33238020250576716 82-83 0.3504482477587612 84-85 0.3785725139170914 86-87 0.3819982595002279 88-89 0.37467710966529916 90-91 0.3471882640586797 92-93 0.32641277471578745 94-95 0.30422830936692774 96-97 0.3205834818283536 98-99 0.3508626524663987 100-101 0.36644426947356784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1809825.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.73258036682894 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86306785447113 45.59911613666028 2 6.876528519162785 7.3898724260141115 3 2.624801147302094 4.231120157830636 4 1.3282634492820704 2.8548409014748106 5 0.7977484862055125 2.1432542323776915 6 0.5638452504484137 1.8178116140504512 7 0.4403981523622317 1.6564610380644613 8 0.3752222956358479 1.61293297245434 9 0.25319658153209074 1.224441509920148 >10 1.6695487120201653 16.809271090290665 >50 0.13392561567091074 4.934195154136909 >100 0.06895464584189345 6.747215861893604 >500 0.0034706027750163876 1.2230500668537263 >1k 8.23133292137607E-4 1.0282235016129375 >5k 2.0555399780141964E-4 0.7281933363653306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6743 0.37257745914660256 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6443 0.35600127084110345 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4657 0.25731769646236513 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4016 0.2218999074496153 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 2725 0.15056704377495062 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002762698050916525 0.0 0.0 2 0.003204729739063169 0.0 0.0 3 0.0035915074661914824 0.0 0.0 4 0.003867777271283135 0.0 0.0 5 0.0050281104526680755 0.0 0.0 6 0.0059674277899796945 0.0 0.0 7 0.006519967400162999 0.0 0.0 8 0.007459284737474618 0.0 0.0 9 0.009061649607006202 0.0 0.0 10-11 0.01768126752586576 0.0 0.0 12-13 0.03237882115674168 0.0 0.0 14-15 0.049811445858024946 0.0 0.0 16-17 0.07160913347975634 0.0 0.0 18-19 0.09064412305057118 0.0 0.0 20-21 0.09785476496346332 0.0 0.0 22-23 0.10340778804580553 0.0 0.0 24-25 0.11017639827055102 0.0 0.0 26-27 0.11810534167668145 0.0 0.0 28-29 0.1277747848548893 0.0 0.0 30-31 0.18501788846987968 0.0 0.0 32-33 0.19584766482947247 0.0 0.0 34-35 0.20380423521611205 0.0 0.0 36-37 0.21129114693409584 0.0 0.0 38-39 0.217838741314768 0.0 0.0 40-41 0.22568480377937095 0.0 0.0 42-43 0.23474645338637712 0.0 0.0 44-45 0.24320030942218168 0.0 0.0 46-47 0.2536433080546462 0.0 0.0 48-49 0.26444545743372977 0.0 0.0 50-51 0.2754133686958684 0.0 0.0 52-53 0.28823228765212106 0.0 0.0 54-55 0.3022115397897587 0.0 0.0 56-57 0.3171577362452171 0.0 0.0 58-59 0.33362341662867956 0.0 0.0 60-61 0.351249430193527 0.0 0.0 62-63 0.3719696655754009 0.0 0.0 64-65 0.39415413092426066 0.0 0.0 66-67 0.4178304532206153 0.0 0.0 68-69 0.44316439434751975 0.0 0.0 70-71 0.47070849391515757 0.0 0.0 72-73 0.5032807039354634 0.0 5.52539610183305E-5 74-75 0.5412125481745472 0.0 5.52539610183305E-5 76-77 0.583647590236625 0.0 5.52539610183305E-5 78-79 0.6320500600886827 0.0 5.52539610183305E-5 80-81 0.6879118146782147 0.0 5.52539610183305E-5 82-83 0.7468401641042642 0.0 5.52539610183305E-5 84-85 0.8126476316770959 0.0 5.52539610183305E-5 86-87 0.8883179312916994 0.0 5.52539610183305E-5 88-89 0.9647341593800506 0.0 5.52539610183305E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1160 0.0 71.13668 9 CGGGAGA 1775 0.0 56.66256 4 AGAGCGG 1585 0.0 53.861893 8 TCGGGAG 1845 0.0 53.741352 3 GGAGAGC 1665 0.0 52.99774 6 ATCGGGA 2125 0.0 51.797855 2 ATTGGGA 815 0.0 47.73522 2 CGGAAAG 850 0.0 45.76712 5 GATCGGG 2555 0.0 44.19456 1 TGTGTTT 39970 0.0 40.57146 1 AAGCGGC 370 0.0 38.45226 9 GATTGGG 1115 0.0 37.870224 1 TTGTGTT 22320 0.0 36.77347 1 AGAGCGT 2925 0.0 34.37545 8 TCGGAAA 710 0.0 33.409527 4 TGGGAGA 1300 0.0 33.20907 4 TTGGGAG 1075 0.0 33.09874 3 CGGAGAG 1250 0.0 32.63977 5 GAGGGGC 2310 0.0 31.821602 9 GAACCGG 345 0.0 31.62944 1 >>END_MODULE