FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809825
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67430.37257745914660256No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64430.35600127084110345No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA46570.25731769646236513No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40160.2218999074496153No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT27250.15056704377495062Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11600.071.136689
CGGGAGA17750.056.662564
AGAGCGG15850.053.8618938
TCGGGAG18450.053.7413523
GGAGAGC16650.052.997746
ATCGGGA21250.051.7978552
ATTGGGA8150.047.735222
CGGAAAG8500.045.767125
GATCGGG25550.044.194561
TGTGTTT399700.040.571461
AAGCGGC3700.038.452269
GATTGGG11150.037.8702241
TTGTGTT223200.036.773471
AGAGCGT29250.034.375458
TCGGAAA7100.033.4095274
TGGGAGA13000.033.209074
TTGGGAG10750.033.098743
CGGAGAG12500.032.639775
GAGGGGC23100.031.8216029
GAACCGG3450.031.629441
Produced by FastQC (version 0.11.2)