##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7491522 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.759249455584595 33.0 33.0 33.0 27.0 33.0 2 31.588251492820817 33.0 33.0 33.0 27.0 33.0 3 31.549611414075805 33.0 33.0 33.0 27.0 33.0 4 35.17006464107027 37.0 37.0 37.0 33.0 37.0 5 35.17020800312673 37.0 37.0 37.0 33.0 37.0 6 35.33129689801351 37.0 37.0 37.0 33.0 37.0 7 35.145605659303946 37.0 37.0 37.0 33.0 37.0 8 35.16244549505428 37.0 37.0 37.0 33.0 37.0 9 34.94513811746131 37.0 37.0 37.0 33.0 37.0 10-11 34.919718382993466 37.0 37.0 37.0 33.0 37.0 12-13 34.945325796280116 37.0 37.0 37.0 33.0 37.0 14-15 36.64289572933244 40.0 37.0 40.0 33.0 40.0 16-17 36.558719242898846 40.0 37.0 40.0 33.0 40.0 18-19 36.55696365838611 40.0 37.0 40.0 33.0 40.0 20-21 36.50164959803895 40.0 37.0 40.0 33.0 40.0 22-23 36.74505821380488 40.0 37.0 40.0 33.0 40.0 24-25 36.39829803610001 40.0 37.0 40.0 33.0 40.0 26-27 36.28279580571211 40.0 37.0 40.0 33.0 40.0 28-29 36.090079559267124 40.0 37.0 40.0 30.0 40.0 30-31 36.13279377675191 40.0 37.0 40.0 33.0 40.0 32-33 36.051947121025606 40.0 37.0 40.0 27.0 40.0 34-35 35.9627657771011 40.0 37.0 40.0 27.0 40.0 36-37 35.867119525244675 40.0 37.0 40.0 27.0 40.0 38-39 35.761465427185556 37.0 37.0 40.0 27.0 40.0 40-41 35.68116685768259 37.0 37.0 40.0 27.0 40.0 42-43 35.56203652608909 37.0 37.0 40.0 27.0 40.0 44-45 35.43626081589295 37.0 37.0 40.0 27.0 40.0 46-47 35.31277442687881 37.0 37.0 40.0 27.0 40.0 48-49 35.17864901150928 37.0 37.0 40.0 27.0 40.0 50-51 34.053463702035444 37.0 35.0 38.5 24.5 38.5 52-53 34.49777067997665 37.0 35.0 38.5 27.0 40.0 54-55 35.228540608971045 37.0 37.0 40.0 27.0 40.0 56-57 35.31985309527223 37.0 37.0 40.0 27.0 40.0 58-59 35.05319332973994 37.0 37.0 40.0 27.0 40.0 60-61 34.83216895044825 37.0 35.0 40.0 27.0 40.0 62-63 34.54931434493551 37.0 33.0 40.0 27.0 40.0 64-65 34.30550053780794 37.0 33.0 40.0 27.0 40.0 66-67 34.069672290890956 37.0 33.0 37.0 27.0 40.0 68-69 33.93882391321817 37.0 33.0 37.0 27.0 40.0 70-71 33.61719634541552 37.0 33.0 37.0 22.0 40.0 72-73 33.51467972729707 37.0 33.0 37.0 27.0 38.5 74-75 33.34312506857752 37.0 33.0 37.0 27.0 37.0 76-77 33.08298060127168 37.0 33.0 37.0 22.0 37.0 78-79 32.790071964014786 37.0 33.0 37.0 22.0 37.0 80-81 32.50362656613703 37.0 33.0 37.0 22.0 37.0 82-83 32.244714358444114 37.0 33.0 37.0 22.0 37.0 84-85 32.058563266583214 35.0 33.0 37.0 22.0 37.0 86-87 31.846851080461352 33.0 33.0 37.0 22.0 37.0 88-89 31.73232134938668 33.0 33.0 37.0 15.0 37.0 90-91 31.62253544740308 33.0 33.0 37.0 15.0 37.0 92-93 31.442317061873407 33.0 33.0 37.0 15.0 37.0 94-95 31.30587616241399 33.0 33.0 37.0 15.0 37.0 96-97 31.14176097994506 33.0 33.0 37.0 15.0 37.0 98-99 31.00492109614041 33.0 33.0 37.0 15.0 37.0 100-101 29.70817732364665 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07151060550819466 2306 2 0.13613900811463253 2306 3 0.06555122028053972 2306 4 0.21196315021619228 2306 5 0.14715916318080957 2306 6 0.0779952773407615 2306 7 0.255497712421608 2306 8 0.08476411440128118 2306 9 0.24575681457640997 2306 10-11 0.23811004210895703 2306 12-13 0.17937998691003543 2306 14-15 0.4441361968891684 2306 16-17 0.4510378488710671 2306 18-19 0.4321595358909178 2306 20-21 0.41976724704611 2306 22-23 0.347705678199695 2306 24-25 0.386105554567429 2306 26-27 0.34202236639097094 2306 28-29 0.30388687481344334 2306 30-31 0.49324998869142433 2306 32-33 0.5992554757492599 2306 34-35 0.7374373069556697 2306 36-37 0.6231709273983128 2306 38-39 0.5466320926291317 2306 40-41 0.5369327007114251 2306 42-43 0.7093078438678688 2306 44-45 0.7029805241108562 2306 46-47 0.5483400053282708 2306 48-49 0.6498775865872091 2306 50-51 0.6075085499808921 2306 52-53 0.6643333597711276 2306 54-55 0.7001295101273257 2306 56-57 0.6668427477279266 2306 58-59 0.6978904744809071 2306 60-61 0.5879841235593872 2306 62-63 0.7362043228103019 2306 64-65 0.6820674498187671 2306 66-67 0.5935104063033521 2306 68-69 0.5689339860815537 2306 70-71 0.5588455201592382 2306 72-73 0.5643788580999782 2306 74-75 0.5176613613799148 2306 76-77 0.48481940177723715 2306 78-79 0.5636308067276019 2306 80-81 0.6243269672029399 2306 82-83 0.5702752328848604 2306 84-85 0.5803557219194033 2306 86-87 0.5963369996494983 2306 88-89 0.5431463592721784 2306 90-91 0.5621206640249525 2306 92-93 0.5458416885519419 2306 94-95 0.5600119419198357 2306 96-97 0.5282310375465471 2306 98-99 0.5151296192050836 2306 100-101 0.46460898252703586 2307 1 0.20915981507088688 2307 2 0.2690800070225734 2307 3 0.2053452703887153 2307 4 0.27634341196212375 2307 5 0.3213155453911867 2307 6 0.25466904000569457 2307 7 0.32752774477240365 2307 8 0.2245918270402143 2307 9 0.33498334286320386 2307 10-11 0.32832505164667936 2307 12-13 0.263517072430254 2307 14-15 0.45406838190350385 2307 16-17 0.4196722722066326 2307 18-19 0.41155917334704384 2307 20-21 0.36945590814660534 2307 22-23 0.2889721479484564 2307 24-25 0.4301378634851645 2307 26-27 0.34346307924642616 2307 28-29 0.22258789227529263 2307 30-31 0.46088271467684194 2307 32-33 0.47305119080937175 2307 34-35 0.43795927373248844 2307 36-37 0.4647420193444347 2307 38-39 0.45465552917842444 2307 40-41 0.5376386656170524 2307 42-43 0.5462982239699912 2307 44-45 0.3754208086777666 2307 46-47 0.4543045751195436 2307 48-49 0.5177115370669441 2307 50-51 0.2506830372594564 2307 52-53 0.3110104689341284 2307 54-55 0.4344161212903259 2307 56-57 0.4684572256189199 2307 58-59 0.5236295882871289 2307 60-61 0.5248784153108019 2307 62-63 0.6433173894036415 2307 64-65 0.6166864587180001 2307 66-67 0.5513972962490001 2307 68-69 0.36097433844764737 2307 70-71 0.4486048607213178 2307 72-73 0.2674073203228744 2307 74-75 0.331074101936629 2307 76-77 0.3513221018915047 2307 78-79 0.36260234134390146 2307 80-81 0.3567755330685074 2307 82-83 0.3692063290032337 2307 84-85 0.42132981757471555 2307 86-87 0.3709093827828305 2307 88-89 0.3694735993713607 2307 90-91 0.36751607232932315 2307 92-93 0.2404849407949321 2307 94-95 0.3326024040447493 2307 96-97 0.36033983705851824 2307 98-99 0.2603905314754691 2307 100-101 -0.04045195376543731 2201 1 0.18444794415770005 2201 2 0.13501053273970953 2201 3 0.0989386114746047 2201 4 0.19848851053549055 2201 5 0.21609319649810743 2201 6 0.17133223077171778 2201 7 0.2968182404716089 2201 8 0.1426584739879999 2201 9 0.22237105035932814 2201 10-11 0.24283419938064554 2201 12-13 0.12892299387650752 2201 14-15 0.2620263886391925 2201 16-17 0.30881392782108463 2201 18-19 0.33818936065280525 2201 20-21 0.3422241506106616 2201 22-23 0.210425729790515 2201 24-25 0.20394756078065512 2201 26-27 0.17543001728719076 2201 28-29 0.2172037885237259 2201 30-31 0.40046066360931576 2201 32-33 0.45563672089748763 2201 34-35 0.4826998437582688 2201 36-37 0.3696298164062952 2201 38-39 0.41711957524099574 2201 40-41 0.33890686455302443 2201 42-43 0.3905600196912289 2201 44-45 0.37829923517303143 2201 46-47 0.41408363937478043 2201 48-49 0.44434669159337403 2201 50-51 0.3250303539824273 2201 52-53 0.40208262534308403 2201 54-55 0.5071766273514626 2201 56-57 0.5024116057642942 2201 58-59 0.6220855268763543 2201 60-61 0.5984237941600341 2201 62-63 0.5272533804481085 2201 64-65 0.53141283149251 2201 66-67 0.5185343200599917 2201 68-69 0.4962356860805741 2201 70-71 0.5264694715932308 2201 72-73 0.490400550191481 2201 74-75 0.4322890287289667 2201 76-77 0.489897208777613 2201 78-79 0.38172667745409683 2201 80-81 0.3641216543701091 2201 82-83 0.39055000892453506 2201 84-85 0.45331347455334026 2201 86-87 0.37247980334719344 2201 88-89 0.40765529199594397 2201 90-91 0.3580591418536301 2201 92-93 0.2477007201492576 2201 94-95 0.26023236998900146 2201 96-97 0.380439998380691 2201 98-99 0.33522532811970507 2201 100-101 0.15686932970207224 1101 1 0.03763954598845132 1101 2 -0.0057142166167096775 1101 3 -0.06560235074158882 1101 4 0.05882499157274168 1101 5 0.057341006488293544 1101 6 0.028441553495468952 1101 7 0.18046194529809867 1101 8 0.022307807194202667 1101 9 0.0738896894402501 1101 10-11 0.04354197076980171 1101 12-13 0.043531980927170366 1101 14-15 0.1648030911648064 1101 16-17 0.14946409543661332 1101 18-19 0.11593345515477438 1101 20-21 0.08755570076833408 1101 22-23 -0.021703676528879612 1101 24-25 -0.09586354751518655 1101 26-27 -0.014902026787261491 1101 28-29 0.03961762153343784 1101 30-31 0.1295450837181633 1101 32-33 0.23257641252273942 1101 34-35 0.1787975144049767 1101 36-37 0.12562178824794756 1101 38-39 0.07348283804849842 1101 40-41 0.19247091463133614 1101 42-43 0.18134852084197206 1101 44-45 0.014425800411885348 1101 46-47 0.12340320746128697 1101 48-49 0.15320230329534468 1101 50-51 0.2601284917780262 1101 52-53 0.3440316559554262 1101 54-55 0.3398295946678047 1101 56-57 0.3373289773853827 1101 58-59 0.4336405273187012 1101 60-61 0.4407820880849016 1101 62-63 0.3280242190856484 1101 64-65 0.25891291145852335 1101 66-67 0.1939479438994809 1101 68-69 0.21216302573428436 1101 70-71 0.24542695539635417 1101 72-73 0.13141274744950238 1101 74-75 0.21579700006693514 1101 76-77 0.25159743426029024 1101 78-79 0.28136825165436363 1101 80-81 0.24926701662660378 1101 82-83 0.21286412231567908 1101 84-85 0.21155196962298817 1101 86-87 0.21097469494612042 1101 88-89 0.2555878312518871 1101 90-91 0.18612284256792933 1101 92-93 0.17211487621554156 1101 94-95 0.10912365727856255 1101 96-97 0.2180183044505739 1101 98-99 0.13642182847527096 1101 100-101 -0.038503366885247914 2304 1 0.060734168450593984 2304 2 0.06341911077408824 2304 3 0.006526665177176483 2304 4 0.10718481063617702 2304 5 0.10791001160119151 2304 6 0.07614808526984973 2304 7 0.21131726114160898 2304 8 0.027904539375050774 2304 9 0.1848761961488563 2304 10-11 0.14786020330291905 2304 12-13 0.07330664149076682 2304 14-15 0.18195057767700717 2304 16-17 0.2188465284001282 2304 18-19 0.11802635459115152 2304 20-21 0.13356017964061806 2304 22-23 0.03037542864542786 2304 24-25 0.10142787479838233 2304 26-27 -0.018831615277200342 2304 28-29 0.05189877766967044 2304 30-31 0.15791132498341653 2304 32-33 0.22353879348232653 2304 34-35 0.23114919835155234 2304 36-37 0.2541754844625501 2304 38-39 0.033466347784248285 2304 40-41 0.15298880172858276 2304 42-43 0.14495616688557078 2304 44-45 0.16297841669535984 2304 46-47 0.20412136494574895 2304 48-49 0.18106996451154345 2304 50-51 0.08070225110193263 2304 52-53 0.16097245261121884 2304 54-55 0.2823393978028008 2304 56-57 0.38381942526878277 2304 58-59 0.45489022188018424 2304 60-61 0.3651834518149428 2304 62-63 0.3697388955957166 2304 64-65 0.31295623702775544 2304 66-67 0.34881303366629623 2304 68-69 0.37743388869490246 2304 70-71 0.34093147971209703 2304 72-73 0.2976359282259864 2304 74-75 0.35851097904861007 2304 76-77 0.26270391935059934 2304 78-79 0.24406616775344503 2304 80-81 0.32292937353367535 2304 82-83 0.26834488759990904 2304 84-85 0.2501568980767317 2304 86-87 0.157306626318249 2304 88-89 0.12233397523917233 2304 90-91 0.08241106022958178 2304 92-93 0.18179602709421872 2304 94-95 0.22052056241235363 2304 96-97 0.2669854754731382 2304 98-99 0.26051668249956705 2304 100-101 0.12476590711191804 2202 1 6.822728762614361E-4 2202 2 0.011482872052997806 2202 3 0.006066284483729589 2202 4 0.07930450025835967 2202 5 0.033463284253571146 2202 6 0.04074218581252609 2202 7 0.1993111365926623 2202 8 -0.013447960118512015 2202 9 0.11495927909029291 2202 10-11 0.0872099189500588 2202 12-13 0.0617570265591354 2202 14-15 0.18398888618412457 2202 16-17 0.16273222876306903 2202 18-19 0.02513683165510372 2202 20-21 0.1305137512558474 2202 22-23 0.13896990746821558 2202 24-25 -0.09871406948373362 2202 26-27 0.037767183907739366 2202 28-29 -0.010068709680410848 2202 30-31 0.14318941742515534 2202 32-33 0.1795718584057795 2202 34-35 0.31249313420465796 2202 36-37 0.2535496966653952 2202 38-39 0.2347168066755998 2202 40-41 0.22113441805804968 2202 42-43 0.17343416659513622 2202 44-45 0.23974862182549117 2202 46-47 0.1572230725389261 2202 48-49 0.22819865227533853 2202 50-51 0.1385888524005523 2202 52-53 0.13843066977106844 2202 54-55 0.26427569030893494 2202 56-57 0.3725249516387805 2202 58-59 0.32529306873469466 2202 60-61 0.3826383150068935 2202 62-63 0.3654975347462752 2202 64-65 0.3961826856477586 2202 66-67 0.38846818083845847 2202 68-69 0.3642148269564487 2202 70-71 0.32389430684125387 2202 72-73 0.2561795850046593 2202 74-75 0.2531538534622868 2202 76-77 0.3153824640546219 2202 78-79 0.3145595857301231 2202 80-81 0.24979260146754 2202 82-83 0.28821730416610336 2202 84-85 0.26119209402155263 2202 86-87 0.25396362285180984 2202 88-89 0.2629016255024723 2202 90-91 0.239057231256524 2202 92-93 0.17215301966505692 2202 94-95 0.18322268353777105 2202 96-97 0.2465496304079302 2202 98-99 0.20546059760081548 2202 100-101 0.20771668928516362 1102 1 -0.15392012392538845 1102 2 -0.2284970769365664 1102 3 -0.23996014672325217 1102 4 -0.15746543167121274 1102 5 -0.1941395270251931 1102 6 -0.292036343185039 1102 7 -0.08307802170102008 1102 8 -0.15894847349645858 1102 9 -0.05696152219091033 1102 10-11 -0.1004898780553134 1102 12-13 -0.17377762066772817 1102 14-15 -0.274308296363138 1102 16-17 -0.23777503919506415 1102 18-19 -0.19608522859368804 1102 20-21 -0.20430915121214355 1102 22-23 -0.23124592879935335 1102 24-25 -0.28152232204627836 1102 26-27 -0.22172031678669413 1102 28-29 -0.3115071702780554 1102 30-31 -0.0790045687432368 1102 32-33 -0.05112953988668778 1102 34-35 -0.044364766681461276 1102 36-37 -0.11004265234502242 1102 38-39 0.03407535348278401 1102 40-41 -0.03757390403897176 1102 42-43 0.04218517226463092 1102 44-45 0.0874012705923306 1102 46-47 -0.03052030481087087 1102 48-49 -0.054065043250432154 1102 50-51 0.10989316295192708 1102 52-53 0.042263095615979296 1102 54-55 0.13251557861870822 1102 56-57 0.2638737041180974 1102 58-59 0.3152979748556817 1102 60-61 0.23354522229421804 1102 62-63 0.279668077739025 1102 64-65 0.21616885339359015 1102 66-67 0.24344290327901064 1102 68-69 0.24129069141102377 1102 70-71 0.23127574233961923 1102 72-73 0.17509170572530053 1102 74-75 0.2335410883347535 1102 76-77 0.2585640808480747 1102 78-79 0.21712854413429739 1102 80-81 0.1545803933409502 1102 82-83 0.15645371628495752 1102 84-85 0.1752824926862786 1102 86-87 0.031365534820796626 1102 88-89 0.026685165013375922 1102 90-91 -0.010429268166973316 1102 92-93 0.14498236502899786 1102 94-95 0.032164220724322234 1102 96-97 0.12033348874739147 1102 98-99 -0.05566170449915475 1102 100-101 -0.06258297010144176 2305 1 -0.010376706462828622 2305 2 -0.03254060476611187 2305 3 0.02035694787149822 2305 4 0.025218500033240332 2305 5 0.0879330057037393 2305 6 0.02841278218503618 2305 7 0.15122841849714064 2305 8 0.05995801722019678 2305 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-0.5021069063823163 1213 98-99 -0.4887447559026086 1213 100-101 -0.3857797315840905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7878.0 3 796.0 4 769.0 5 659.0 6 1309.0 7 5211.0 8 24930.0 9 62359.0 10 80434.0 11 54889.0 12 32817.0 13 23979.0 14 20484.0 15 20417.0 16 21674.0 17 21857.0 18 23063.0 19 24854.0 20 26947.0 21 30010.0 22 32847.0 23 35662.0 24 40350.0 25 46015.0 26 53518.0 27 64385.0 28 78794.0 29 97372.0 30 119713.0 31 154873.0 32 210413.0 33 300083.0 34 457889.0 35 774993.0 36 1497966.0 37 2375860.0 38 664823.0 39 630.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.44473693293971 26.2134296101522 22.360947630615748 14.980885826292337 2 32.53481302255328 24.40985425277064 29.78648869218219 13.268844032493893 3 36.478805518720556 20.3413988593103 26.062921484202334 17.116874137766803 4 30.842226195018142 19.190561856914066 31.18717629992386 18.780035648143937 5 30.967149307718888 17.86335577232714 32.777310791146114 18.39218412880786 6 27.520905820068435 18.199902875971226 36.469818554168725 17.809372749791613 7 33.552151958042266 18.882705840846445 28.24721625702951 19.317925944081775 8 29.878861040144837 27.832290042167745 23.641641323480194 18.647207594207227 9 29.337908186018563 27.35116190039998 24.08370744449328 19.227222469088176 10-11 25.048930418630928 28.625873083976554 28.67133952053138 17.653856976861135 12-13 25.72298267691065 25.790514237179323 28.546993865638015 19.93950922027202 14-15 24.627943652671693 26.536079790466683 28.877002450343326 19.958974106518298 16-17 24.450512349011504 27.537857198327526 29.60578828330021 18.40584216936076 18-19 23.824159437423205 27.35816196234132 30.02710314202223 18.790575458213247 20-21 23.08991825722414 28.643256105629412 29.49261750962908 18.774208127517365 22-23 24.472804080728334 27.61090224835038 28.82274453289077 19.09354913803051 24-25 23.154760942517928 28.41653492069316 29.141710729375536 19.286993407413373 26-27 22.093210949594738 29.360188598947673 29.18140458742105 19.365195864036544 28-29 22.409293307908328 29.308316369618336 28.609864243779583 19.672526078693753 30-31 22.83591668983278 28.95988566384442 28.764580591563465 19.439617054759335 32-33 22.781416024232083 28.553496759340298 29.30130729685512 19.3637799195725 34-35 21.980997489769365 28.457971322061947 28.95690913651067 20.604122051658017 36-37 20.49181519500116 29.129720354421977 29.411139288437386 20.96732516213947 38-39 20.567026114046048 29.376786158452273 29.377938383683897 20.67824934381778 40-41 21.393414556972836 28.24562659934958 29.985788620995123 20.375170222682453 42-43 22.224904932299516 28.22711197896261 30.01359579890283 19.534387289835045 44-45 21.176606794328006 28.91460970867334 30.046231682078677 19.862551814919975 46-47 22.112291568955058 29.237122054185622 29.03070555201622 19.619880824843104 48-49 21.500270152386317 29.056877681713917 29.12499374290486 20.31785842299491 50-51 20.77544094101373 28.637491094723988 29.187282562145928 21.399785402116358 52-53 22.735241408324192 28.37407960393404 28.179345474832584 20.711333512909185 54-55 23.67420326504786 28.07452464838982 28.256971066521285 19.994301020041032 56-57 23.939642216086774 27.58727741531012 28.11557253044984 20.357507838153268 58-59 22.997364493050203 27.659140207736804 28.563013488981824 20.780481810231173 60-61 21.372162384638415 27.84049542438395 28.214240503997594 22.573101686980042 62-63 22.08186343596113 27.966938607444504 28.46755290124097 21.483645055353396 64-65 21.49931207259027 28.6591550065471 29.287317383132706 20.554215537729924 66-67 21.50238312710777 28.247442276790764 28.73687397995991 21.513300616141553 68-69 21.14404913517316 27.488202157794266 30.606044492116823 20.761704214915753 70-71 20.926799596810774 29.460310935084994 29.50526298522722 20.107626482877013 72-73 21.026928665266002 31.133693514016358 28.003369731647897 19.83600808906974 74-75 21.8131494005379 30.791603312557875 27.527171129407087 19.868076157497143 76-77 21.926038542973867 29.927142533261325 27.74502752409555 20.401791399669257 78-79 21.645705548997533 29.84074452347107 28.334727526898828 20.17882240063257 80-81 21.096861781051963 30.581421420230853 28.07112887321623 20.25058792550095 82-83 20.44995932185861 30.46181244864996 28.36345534185781 20.724772887633616 84-85 20.62614639804548 30.023461293957094 28.562041641786724 20.788350666210697 86-87 20.48048126117185 30.222235682307268 29.05256396976144 20.244719086759435 88-89 20.536505346749472 30.557566999854746 28.90369526288341 20.00223239051237 90-91 21.05266083860767 29.597111610424275 28.68818705516107 20.662040495806988 92-93 20.72387435626416 30.03698350461229 28.174502301725614 21.064639837397937 94-95 20.44297063102199 30.55851156597705 27.770723894463075 21.227793908537887 96-97 20.757840009395945 30.915805985522265 27.47170201462398 20.85465199045781 98-99 21.067282237333142 30.00237182166018 27.790067057379797 21.14027888362688 100-101 21.341322321911516 30.124399697033155 27.30334588638636 21.230932094668965 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7726.0 1 4923.0 2 1715.0 3 1487.0 4 1527.5 5 1421.0 6 1676.0 7 2135.5 8 2483.0 9 2798.5 10 3130.0 11 3233.5 12 3145.0 13 2996.5 14 2731.5 15 2490.0 16 2408.5 17 2445.5 18 2592.5 19 2961.5 20 3520.5 21 4351.5 22 5652.5 23 7395.5 24 9913.5 25 13107.0 26 17244.5 27 22668.0 28 28821.5 29 35801.5 30 44039.5 31 54531.0 32 69521.0 33 91288.0 34 121820.0 35 171084.5 36 239618.5 37 319656.5 38 397099.5 39 435376.0 40 452907.0 41 441396.5 42 416161.5 43 430014.0 44 431847.5 45 423585.5 46 438260.5 47 410419.5 48 331112.5 49 265877.0 50 223970.5 51 200223.0 52 182293.5 53 156473.0 54 124925.5 55 93938.5 56 72055.0 57 59665.0 58 49895.5 59 38325.5 60 27984.5 61 20795.5 62 15086.5 63 10819.0 64 7975.5 65 5627.5 66 4042.5 67 3032.5 68 2239.0 69 1508.0 70 1008.0 71 732.0 72 525.0 73 411.5 74 342.0 75 268.5 76 206.0 77 167.0 78 132.0 79 113.5 80 96.0 81 78.0 82 57.5 83 44.0 84 34.5 85 28.0 86 28.0 87 22.0 88 19.5 89 21.0 90 13.5 91 8.0 92 8.0 93 8.0 94 6.0 95 5.5 96 3.5 97 2.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22644263742401077 2 0.24348857281604458 3 0.22353268134299012 4 0.21675168276886858 5 0.2253881120552005 6 0.24832070172122567 7 0.2288320050318213 8 0.2154435373746483 9 0.20796842083624661 10-11 0.19459330160146363 12-13 0.22022894680146435 14-15 0.2168184248808186 16-17 0.22012215942234437 18-19 0.20871593249008677 20-21 0.20131423227483014 22-23 0.1690043758798279 24-25 0.500132015897437 26-27 0.3519845500019889 28-29 0.4858024844617689 30-31 0.316597882246091 32-33 0.36751543945275744 34-35 0.3692974538418228 36-37 0.3773799235989696 38-39 0.36979801968144793 40-41 0.3755778865763192 42-43 0.4302530246857715 44-45 0.38420096744026117 46-47 0.4024749576921753 48-49 0.4003525585321648 50-51 0.41456195416632297 52-53 0.41847971613778884 54-55 0.38420764165145616 56-57 0.39756273825265415 58-59 0.39484633429628857 60-61 0.352491790052809 62-63 0.3149827231369006 64-65 0.34195988478709666 66-67 0.3528722200909241 68-69 0.3437218765425771 70-71 0.31454889940922554 72-73 0.30271552296048787 74-75 0.3147157546891005 76-77 0.321229784815422 78-79 0.3315748121676743 80-81 0.33958386560167614 82-83 0.3580714306118303 84-85 0.38734452091310684 86-87 0.38882619579839717 88-89 0.38269926992138575 90-91 0.3550813839964696 92-93 0.33304313863057466 94-95 0.3099036484175045 96-97 0.32664924430576325 98-99 0.3576776521513252 100-101 0.3737424784976938 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7491522.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.51226387833716 #Duplication Level Percentage of deduplicated Percentage of total 1 89.43720026939069 40.70489459200196 2 5.099155440332379 4.641482159141315 3 1.6926107243613135 2.311036377913065 4 0.9160290901265528 1.6676223068029103 5 0.47417938025096174 1.0790488539824072 6 0.3587031910639928 0.9795236571423619 7 0.20276668572225937 0.645985963442913 8 0.19589322378506469 0.7132435274307214 9 0.1361192807382823 0.5575586961501144 >10 1.1431733436323555 10.438882173706936 >50 0.16236104534178192 5.131036192854845 >100 0.14978839712950953 14.07764261340719 >500 0.019325224239126814 6.178463947428809 >1k 0.012369582513847415 9.649114019282669 >5k 2.069733414668535E-4 0.6172196030885561 >10k+ 1.1814803037906128E-4 0.60724531622332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13879 0.18526275435085154 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10481 0.13990481506962138 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10480 0.13989146664723134 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 10332 0.1379159001335109 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9746 0.13009372461296917 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9010 0.12026928573392696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002055657048060461 0.0 0.0 2 0.0022558833839105056 0.0 0.0 3 0.002456109719760551 0.0 0.0 4 0.0027898202795106253 0.0 0.0 5 0.003350454019890751 0.0 0.0 6 0.0038710424931008677 0.0 0.0 7 0.004725341526061059 0.0 0.0 8 0.005566292136631248 0.0 0.0 9 0.0062337132561313974 0.0 0.0 10-11 0.010645366856027386 0.0 0.0 12-13 0.014236092478938192 0.0 6.674211195001496E-6 14-15 0.019448651422234363 1.3348422390002993E-5 1.3348422390002993E-5 16-17 0.0258625683806308 1.3348422390002993E-5 1.3348422390002993E-5 18-19 0.033477843354127505 1.3348422390002993E-5 1.3348422390002993E-5 20-21 0.03859696334069365 1.3348422390002993E-5 2.6696844780005985E-5 22-23 0.04368271227128479 1.3348422390002993E-5 2.6696844780005985E-5 24-25 0.049309072308671056 1.3348422390002993E-5 2.6696844780005985E-5 26-27 0.05558950504316746 1.3348422390002993E-5 3.337105597500748E-5 28-29 0.06307797000395915 1.3348422390002993E-5 4.0045267170008976E-5 30-31 0.11169292434835004 1.3348422390002993E-5 4.0045267170008976E-5 32-33 0.12269202439771251 1.3348422390002993E-5 4.0045267170008976E-5 34-35 0.13141521842957946 1.3348422390002993E-5 4.0045267170008976E-5 36-37 0.14065900093465653 1.3348422390002993E-5 5.339368956001197E-5 38-39 0.1507370598391088 1.3348422390002993E-5 5.339368956001197E-5 40-41 0.16204317360344134 2.0022633585004488E-5 6.674211195001496E-5 42-43 0.17447055484853413 2.6696844780005985E-5 6.674211195001496E-5 44-45 0.18795913567363215 2.6696844780005985E-5 6.674211195001496E-5 46-47 0.20223527341974035 2.6696844780005985E-5 6.674211195001496E-5 48-49 0.2167049632905036 2.6696844780005985E-5 7.341632314501646E-5 50-51 0.23391107975121744 2.6696844780005985E-5 8.009053434001795E-5 52-53 0.25194479840011147 2.6696844780005985E-5 9.343895673002094E-5 54-55 0.27232783938964605 2.6696844780005985E-5 1.0678737912002394E-4 56-57 0.29385217049352585 2.6696844780005985E-5 1.0678737912002394E-4 58-59 0.3176657560372912 2.6696844780005985E-5 1.0678737912002394E-4 60-61 0.3422001563901167 2.6696844780005985E-5 1.0678737912002394E-4 62-63 0.3697846712590579 2.6696844780005985E-5 1.0678737912002394E-4 64-65 0.3995516531887646 2.6696844780005985E-5 1.0678737912002394E-4 66-67 0.43242881753534196 2.6696844780005985E-5 1.0678737912002394E-4 68-69 0.46812249900620995 2.6696844780005985E-5 1.0678737912002394E-4 70-71 0.5078474040388589 2.6696844780005985E-5 1.2013580151002693E-4 72-73 0.5512831704959287 2.6696844780005985E-5 1.2013580151002693E-4 74-75 0.5989170157946542 2.6696844780005985E-5 1.2013580151002693E-4 76-77 0.6513896642097561 2.6696844780005985E-5 1.2013580151002693E-4 78-79 0.7107634470004893 2.6696844780005985E-5 1.2013580151002693E-4 80-81 0.776177390922699 2.6696844780005985E-5 1.2013580151002693E-4 82-83 0.8488595508362653 2.6696844780005985E-5 1.2013580151002693E-4 84-85 0.9307521221989337 2.6696844780005985E-5 1.2013580151002693E-4 86-87 1.021741643420389 2.6696844780005985E-5 1.2013580151002693E-4 88-89 1.119231846345776 2.6696844780005985E-5 1.2013580151002693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 4240 0.0 76.84511 2 GAGCGGC 2825 0.0 76.5347 9 CGGGAGA 3965 0.0 71.16892 4 AGAGCGG 3330 0.0 67.06657 8 CGGAAAG 1955 0.0 61.374844 5 GTTCGGA 805 0.0 57.14802 2 TCGGGAG 5085 0.0 55.6801 3 AAGCGGC 1105 0.0 54.494595 9 TCGGAGA 1145 0.0 54.26033 4 GGTCCGG 570 0.0 53.25739 1 GATCGGG 6565 0.0 53.248516 1 ATTCGGA 680 0.0 53.00661 2 GGTTCGG 1070 0.0 52.751884 1 TCGGGAA 1515 0.0 51.652092 3 GGATCGG 1855 0.0 50.372913 1 GAGAGCG 5165 0.0 49.758152 7 TCGGAAA 1715 0.0 48.117355 4 GATCCGG 1170 0.0 46.21615 1 CGGAGAG 2210 0.0 45.065445 5 GAACCGG 475 0.0 44.93592 1 >>END_MODULE