FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491522
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA138790.18526275435085154No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG104810.13990481506962138No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC104800.13989146664723134No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT103320.1379159001335109Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC97460.13009372461296917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90100.12026928573392696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA42400.076.845112
GAGCGGC28250.076.53479
CGGGAGA39650.071.168924
AGAGCGG33300.067.066578
CGGAAAG19550.061.3748445
GTTCGGA8050.057.148022
TCGGGAG50850.055.68013
AAGCGGC11050.054.4945959
TCGGAGA11450.054.260334
GGTCCGG5700.053.257391
GATCGGG65650.053.2485161
ATTCGGA6800.053.006612
GGTTCGG10700.052.7518841
TCGGGAA15150.051.6520923
GGATCGG18550.050.3729131
GAGAGCG51650.049.7581527
TCGGAAA17150.048.1173554
GATCCGG11700.046.216151
CGGAGAG22100.045.0654455
GAACCGG4750.044.935921
Produced by FastQC (version 0.11.2)