##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_35011-1.342000000358a4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5928097 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.462051818652764 33.0 33.0 33.0 27.0 33.0 2 31.220732892865957 33.0 33.0 33.0 27.0 33.0 3 31.131156592073307 33.0 33.0 33.0 27.0 33.0 4 34.67382618739201 37.0 37.0 37.0 33.0 37.0 5 34.668774987993615 37.0 37.0 37.0 33.0 37.0 6 34.892831038358516 37.0 37.0 37.0 33.0 37.0 7 34.68145190606699 37.0 37.0 37.0 33.0 37.0 8 34.738934433765166 37.0 37.0 37.0 33.0 37.0 9 34.4333166275788 37.0 37.0 37.0 27.0 37.0 10-11 34.42505773775294 37.0 37.0 37.0 27.0 37.0 12-13 34.44863646799301 37.0 37.0 37.0 27.0 37.0 14-15 36.13731801284628 40.0 37.0 40.0 27.0 40.0 16-17 36.07311064579409 40.0 37.0 40.0 27.0 40.0 18-19 36.10096132030228 40.0 37.0 40.0 27.0 40.0 20-21 36.053551839654446 40.0 37.0 40.0 27.0 40.0 22-23 36.42109584239259 40.0 37.0 40.0 27.0 40.0 24-25 36.03758010369938 40.0 37.0 40.0 27.0 40.0 26-27 35.89473308213411 40.0 37.0 40.0 27.0 40.0 28-29 35.693386427381334 40.0 37.0 40.0 27.0 40.0 30-31 35.73008606303169 40.0 37.0 40.0 27.0 40.0 32-33 35.59417086123928 40.0 37.0 40.0 27.0 40.0 34-35 35.609735721260975 40.0 37.0 40.0 27.0 40.0 36-37 35.5004368180885 40.0 37.0 40.0 27.0 40.0 38-39 35.42118651229897 40.0 37.0 40.0 27.0 40.0 40-41 35.28352403815255 38.5 37.0 40.0 24.5 40.0 42-43 35.19539541947441 37.0 37.0 40.0 22.0 40.0 44-45 35.1050795896221 37.0 37.0 40.0 22.0 40.0 46-47 35.00535213914347 37.0 37.0 40.0 22.0 40.0 48-49 34.8891495027831 37.0 37.0 40.0 22.0 40.0 50-51 33.84790296110202 37.0 35.0 38.5 22.0 38.5 52-53 34.27149901562002 37.0 35.0 38.5 22.0 40.0 54-55 35.02388785136276 37.0 37.0 40.0 22.0 40.0 56-57 35.187889300731754 37.0 37.0 40.0 24.5 40.0 58-59 34.89355774711514 37.0 37.0 40.0 22.0 40.0 60-61 34.66914500555575 37.0 37.0 40.0 22.0 40.0 62-63 34.393546107629476 37.0 33.0 40.0 22.0 40.0 64-65 34.1614594025705 37.0 33.0 40.0 22.0 40.0 66-67 33.94878398582209 37.0 33.0 38.5 22.0 40.0 68-69 33.86116927573891 37.0 33.0 37.0 22.0 40.0 70-71 33.553024857724154 37.0 33.0 37.0 22.0 40.0 72-73 33.503222028924284 37.0 33.0 37.0 22.0 40.0 74-75 33.401480947427146 37.0 33.0 37.0 22.0 38.5 76-77 33.16009766709283 37.0 33.0 37.0 22.0 37.0 78-79 32.822848209130186 37.0 33.0 37.0 22.0 37.0 80-81 32.46871103829778 37.0 33.0 37.0 22.0 37.0 82-83 32.18558282700165 37.0 33.0 37.0 22.0 37.0 84-85 31.98544457015464 37.0 33.0 37.0 15.0 37.0 86-87 31.763081980608618 37.0 33.0 37.0 15.0 37.0 88-89 31.621280319805834 33.0 33.0 37.0 15.0 37.0 90-91 31.50356514071885 33.0 33.0 37.0 15.0 37.0 92-93 31.34498364314889 33.0 33.0 37.0 15.0 37.0 94-95 31.222906271607904 33.0 33.0 37.0 15.0 37.0 96-97 31.054415776260072 33.0 33.0 37.0 15.0 37.0 98-99 30.93118466178944 33.0 33.0 37.0 15.0 37.0 100-101 29.681214393084325 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.03844119836126225 2306 2 -0.04280446072182542 2306 3 -0.07657062738158871 2306 4 0.009040941739115738 2306 5 0.03447730655323511 2306 6 -0.053325357479522495 2306 7 0.20381534223473352 2306 8 -0.0786915335418712 2306 9 0.10505220338138344 2306 10-11 0.10200993160266592 2306 12-13 0.017230107382424364 2306 14-15 0.19929650190614012 2306 16-17 0.21974059044159588 2306 18-19 0.2381740171759148 2306 20-21 0.23693559847776413 2306 22-23 0.15107754283078378 2306 24-25 0.06169264027520427 2306 26-27 -0.013559271820589913 2306 28-29 0.011879541547081374 2306 30-31 0.21757053517870162 2306 32-33 0.27063132034717086 2306 34-35 0.2767289476960144 2306 36-37 0.3738303120044648 2306 38-39 0.2554612323149854 2306 40-41 0.25435968627878935 2306 42-43 0.3237330096149975 2306 44-45 0.3107182964654953 2306 46-47 0.341354654721286 2306 48-49 0.3818931674706505 2306 50-51 0.34476000956687614 2306 52-53 0.2433075559030229 2306 54-55 0.3753452014376535 2306 56-57 0.3594667324288139 2306 58-59 0.4448608245136825 2306 60-61 0.434669541763661 2306 62-63 0.43692881367756087 2306 64-65 0.3825825792336346 2306 66-67 0.32888800091193815 2306 68-69 0.2149748660959503 2306 70-71 0.2644991786066768 2306 72-73 0.18548931949251113 2306 74-75 0.20967451580233387 2306 76-77 0.1741435809552172 2306 78-79 0.2673738195025024 2306 80-81 0.2580275753436396 2306 82-83 0.33421307160381275 2306 84-85 0.3859550541508341 2306 86-87 0.3176529722017776 2306 88-89 0.29676712205781897 2306 90-91 0.314935014380584 2306 92-93 0.279732634960947 2306 94-95 0.29922224429359545 2306 96-97 0.17118523984894551 2306 98-99 0.18242730023500187 2306 100-101 -0.05849989954345247 2307 1 0.06653856910710942 2307 2 0.05044186322665212 2307 3 -0.024015439114556614 2307 4 0.16950977287505253 2307 5 0.16378851391927185 2307 6 0.11019038526653446 2307 7 0.25457255818771785 2307 8 0.09667876294862054 2307 9 0.22424318708137037 2307 10-11 0.22354198385895785 2307 12-13 0.09013521712569883 2307 14-15 0.3290433102955248 2307 16-17 0.2737515552705503 2307 18-19 0.2920421827597153 2307 20-21 0.21438970324365414 2307 22-23 0.2861721196257534 2307 24-25 0.31552803485705994 2307 26-27 0.08945953905115545 2307 28-29 0.023152053800586714 2307 30-31 0.2963627100333639 2307 32-33 0.3128098162373405 2307 34-35 0.3897759226317348 2307 36-37 0.49301678375490354 2307 38-39 0.3444187503311227 2307 40-41 0.3858580809746357 2307 42-43 0.42927067568639643 2307 44-45 0.28338419372602885 2307 46-47 0.34641437076925996 2307 48-49 0.30089290783566014 2307 50-51 0.08511654856911122 2307 52-53 0.21381974924877056 2307 54-55 0.2778141877213258 2307 56-57 0.32710885092368613 2307 58-59 0.4516579577634303 2307 60-61 0.4781692472995971 2307 62-63 0.5198095272305068 2307 64-65 0.48772905136403466 2307 66-67 0.39130045325941865 2307 68-69 0.22486363466618542 2307 70-71 0.3526011676640124 2307 72-73 0.19457242801993146 2307 74-75 0.15725832724400846 2307 76-77 0.22457957776360615 2307 78-79 0.11960755115391208 2307 80-81 0.33507797936101724 2307 82-83 0.26291009973304114 2307 84-85 0.3712681042920565 2307 86-87 0.2968846267732559 2307 88-89 0.2941888199440079 2307 90-91 0.34010987221459743 2307 92-93 0.2890732412403132 2307 94-95 0.33370960766859525 2307 96-97 0.27603036124413194 2307 98-99 0.22791202609844774 2307 100-101 0.1413391742562098 2201 1 0.17918453730236195 2201 2 0.19651205488924006 2201 3 0.1555549066759383 2201 4 0.27307654954673666 2201 5 0.2498470402147035 2201 6 0.10387605197550442 2201 7 0.2989882169787492 2201 8 0.16122473537483728 2201 9 0.35590808581027034 2201 10-11 0.2870107608691228 2201 12-13 0.14621038772414607 2201 14-15 0.3843383972417911 2201 16-17 0.4330187601760471 2201 18-19 0.42866580694147416 2201 20-21 0.4399704405794722 2201 22-23 0.2555161542712838 2201 24-25 0.32496100281658613 2201 26-27 0.2866580754404495 2201 28-29 0.3879733787566195 2201 30-31 0.5189908401898506 2201 32-33 0.5525833539865843 2201 34-35 0.5264129574525498 2201 36-37 0.6828836671072835 2201 38-39 0.5546890710380552 2201 40-41 0.49549572172968936 2201 42-43 0.5692384599315048 2201 44-45 0.5760101932908483 2201 46-47 0.5764102304705503 2201 48-49 0.6225778734137606 2201 50-51 0.4554649456388731 2201 52-53 0.541893656602781 2201 54-55 0.711753946375147 2201 56-57 0.6766620688726377 2201 58-59 0.8139565715188937 2201 60-61 0.6798099162147153 2201 62-63 0.6587411035056903 2201 64-65 0.6912594870926938 2201 66-67 0.6021160769939158 2201 68-69 0.5085507822119268 2201 70-71 0.5701680093111889 2201 72-73 0.42110003202605384 2201 74-75 0.29380583188294906 2201 76-77 0.4580210272912453 2201 78-79 0.4640118392340824 2201 80-81 0.4911141646650705 2201 82-83 0.4872793463800065 2201 84-85 0.49487830876841343 2201 86-87 0.5292009047958288 2201 88-89 0.5567645994987878 2201 90-91 0.5264537240221223 2201 92-93 0.32977906395090884 2201 94-95 0.35304831583495755 2201 96-97 0.46440809701449837 2201 98-99 0.46737013050650233 2201 100-101 0.20212657898400366 1101 1 0.017708644952730168 1101 2 -0.11166416568249815 1101 3 -0.14429661028805185 1101 4 0.013767078361013318 1101 5 -0.06045145152978648 1101 6 -0.11227055839879796 1101 7 0.18508596532920052 1101 8 -0.07357912795191623 1101 9 0.029422276589535556 1101 10-11 -0.020476797474351827 1101 12-13 -0.04655630455041404 1101 14-15 0.09661892993100452 1101 16-17 -0.02259134342116198 1101 18-19 0.004896606083320876 1101 20-21 0.03196266842583384 1101 22-23 -0.08042528015787553 1101 24-25 -0.162079050811478 1101 26-27 -0.1514094105888475 1101 28-29 -0.1561707054821042 1101 30-31 -0.07778042167630161 1101 32-33 -0.05155712534460832 1101 34-35 -0.026528805738777805 1101 36-37 0.05515332094164904 1101 38-39 -0.06289859099714334 1101 40-41 -0.018769653470982917 1101 42-43 -0.09113292317835686 1101 44-45 -0.06034805013357669 1101 46-47 -0.04371960020680632 1101 48-49 -0.046216858830725016 1101 50-51 0.01568546606871024 1101 52-53 0.07789640483846938 1101 54-55 0.27636491001959484 1101 56-57 0.14703136599668198 1101 58-59 0.26210927580711285 1101 60-61 0.22771054700601923 1101 62-63 0.1553927249714988 1101 64-65 0.18813776543206018 1101 66-67 0.21178838482330775 1101 68-69 0.09819079101492179 1101 70-71 0.16366211319463275 1101 72-73 0.07110949997140636 1101 74-75 0.01698302456458123 1101 76-77 0.09335416732772472 1101 78-79 0.1982549672947087 1101 80-81 0.06916480078948695 1101 82-83 0.07211238988057289 1101 84-85 0.07316420898943221 1101 86-87 0.04804675719443452 1101 88-89 0.02084075080769665 1101 90-91 0.07209124584396776 1101 92-93 0.050793818418899406 1101 94-95 0.05895207763022725 1101 96-97 0.0935066541063776 1101 98-99 0.024855494851944826 1101 100-101 0.026419909094975935 2304 1 -0.018279385737727694 2304 2 -0.051305831531795576 2304 3 -0.005352938731626011 2304 4 0.08741368795638493 2304 5 0.06188075421581374 2304 6 -0.0862341927440653 2304 7 0.09547068336003406 2304 8 -0.11756280492198101 2304 9 0.11653747682189675 2304 10-11 0.06846451702073608 2304 12-13 -0.004088084960500282 2304 14-15 0.05285909775377462 2304 16-17 0.07726360226377693 2304 18-19 0.06707109401174449 2304 20-21 0.04947053987018535 2304 22-23 -0.059367123090915186 2304 24-25 -0.00240287682385798 2304 26-27 -0.09252262404660172 2304 28-29 -0.04035486192407234 2304 30-31 0.009697689967801182 2304 32-33 0.10978581415419342 2304 34-35 0.11016161022172355 2304 36-37 0.1324741329568795 2304 38-39 -0.046160585207893234 2304 40-41 0.0789352721029033 2304 42-43 -0.015163429634803549 2304 44-45 0.09501857935234881 2304 46-47 0.10527231887451904 2304 48-49 0.025782240923916788 2304 50-51 -0.06041752458592242 2304 52-53 0.034630681148414055 2304 54-55 0.18205227092446563 2304 56-57 0.18148861840830222 2304 58-59 0.26510283874863205 2304 60-61 0.22222788927489745 2304 62-63 0.21233510698726832 2304 64-65 0.19285878821420255 2304 66-67 0.2198977022867794 2304 68-69 0.1842818100789856 2304 70-71 0.19167443758406222 2304 72-73 0.18922288906824036 2304 74-75 0.2243990618578593 2304 76-77 0.21851319293377713 2304 78-79 0.12847066882078906 2304 80-81 0.10206228686951846 2304 82-83 0.153634948858425 2304 84-85 0.19577757033378163 2304 86-87 0.12378453640165787 2304 88-89 0.09630336211231594 2304 90-91 0.14994754797968923 2304 92-93 0.18452728192284695 2304 94-95 0.20496588772864044 2304 96-97 0.33458981503208207 2304 98-99 0.12424623251330047 2304 100-101 -0.019246747687240884 2202 1 0.03885955296342658 2202 2 0.021917940692883775 2202 3 0.036996544126484565 2202 4 0.12604989280512058 2202 5 0.1336797662509781 2202 6 0.06488199016139617 2202 7 0.2266381534920825 2202 8 -0.016948755534087923 2202 9 0.17470940547520541 2202 10-11 0.053883108252229306 2202 12-13 0.07139962240731279 2202 14-15 0.14514945280526348 2202 16-17 0.15763161787248947 2202 18-19 0.07210045090304362 2202 20-21 0.1388047633551821 2202 22-23 0.06883878140927635 2202 24-25 -0.035408613644300146 2202 26-27 0.018202409657540386 2202 28-29 -0.0031687762366274796 2202 30-31 0.1908572677668019 2202 32-33 0.20329805762101927 2202 34-35 0.16893904901732526 2202 36-37 0.3299071839775749 2202 38-39 0.2102636445133399 2202 40-41 0.18887055497188499 2202 42-43 0.09762214990826834 2202 44-45 0.15922671958228563 2202 46-47 0.10344854302712747 2202 48-49 0.11845453314207077 2202 50-51 0.1502635190651418 2202 52-53 0.11116716313448194 2202 54-55 0.15206761569003646 2202 56-57 0.2878938289006996 2202 58-59 0.23975913411337757 2202 60-61 0.22012542350304187 2202 62-63 0.20738130948124933 2202 64-65 0.27149307820180724 2202 66-67 0.29616895012932076 2202 68-69 0.283478603462882 2202 70-71 0.25389526247519 2202 72-73 0.11003173676174782 2202 74-75 0.19450135166950133 2202 76-77 0.07846827132664203 2202 78-79 0.19219802364715122 2202 80-81 0.22612820984551973 2202 82-83 0.13248608393118388 2202 84-85 0.1627625179726735 2202 86-87 0.21980007265334223 2202 88-89 0.1951720414532936 2202 90-91 0.1648966618388883 2202 92-93 0.1376812393456497 2202 94-95 0.15567943226598047 2202 96-97 0.10340009110847603 2202 98-99 0.023029001868273724 2202 100-101 0.021197965872822522 1102 1 -0.05302301430057099 1102 2 -0.1808190199189923 1102 3 -0.16334214763983468 1102 4 -0.1054466088176369 1102 5 -0.11998368333367182 1102 6 -0.09675705175072125 1102 7 0.04848369342644787 1102 8 -0.08013159553043181 1102 9 0.010106249755196473 1102 10-11 0.05826057057280565 1102 12-13 -0.048582085899106175 1102 14-15 -0.12001859995722697 1102 16-17 -0.04823347828055802 1102 18-19 -0.04869373531487042 1102 20-21 -0.07429454120341461 1102 22-23 -0.1097321314486095 1102 24-25 -0.16499575188846194 1102 26-27 -0.13127284560542307 1102 28-29 -0.13451953518076465 1102 30-31 0.01960028337477837 1102 32-33 0.048483770130516746 1102 34-35 0.09956540760371979 1102 36-37 0.016709485337422336 1102 38-39 0.014633934240308122 1102 40-41 0.03592327464085798 1102 42-43 0.01472448844236851 1102 44-45 0.02134160146594155 1102 46-47 0.026699737524644718 1102 48-49 0.06595727776503679 1102 50-51 0.17175965573876084 1102 52-53 0.09947480862951608 1102 54-55 0.17097518860600758 1102 56-57 0.2223393250095711 1102 58-59 0.3012074703481318 1102 60-61 0.21963334250455802 1102 62-63 0.35919615735038235 1102 64-65 0.29759640557430345 1102 66-67 0.30423747020049774 1102 68-69 0.27696163862012213 1102 70-71 0.23191941515727166 1102 72-73 0.13333745244992912 1102 74-75 0.11658606758344803 1102 76-77 0.17544737711907743 1102 78-79 0.13023506171987975 1102 80-81 0.20268248639387565 1102 82-83 0.20940011186543472 1102 84-85 0.1834421164780622 1102 86-87 0.08410496538910195 1102 88-89 0.03524469003143693 1102 90-91 0.11764389948192289 1102 92-93 0.09659058803149279 1102 94-95 0.10120304660857826 1102 96-97 0.19924415765940395 1102 98-99 0.10871656527029216 1102 100-101 0.11765800654806569 2305 1 -0.023701959516269966 2305 2 -0.08042910807695591 2305 3 -0.029509318380995353 2305 4 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1213 100-101 -0.06958843436056128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6384.0 3 701.0 4 728.0 5 545.0 6 1429.0 7 7407.0 8 31207.0 9 78170.0 10 95611.0 11 62297.0 12 38414.0 13 25995.0 14 19330.0 15 15496.0 16 14364.0 17 14030.0 18 15254.0 19 16440.0 20 18306.0 21 20789.0 22 22642.0 23 24914.0 24 28145.0 25 32372.0 26 37928.0 27 45396.0 28 55357.0 29 68533.0 30 84242.0 31 109593.0 32 148601.0 33 211989.0 34 321557.0 35 539019.0 36 1051476.0 37 1929013.0 38 733330.0 39 1093.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.47836225512175 24.875358690366774 23.315989728419908 15.33028932609156 2 30.54802069863385 24.582020475758785 31.976348863396353 12.89360996221101 3 37.031832253638214 20.829067893738184 26.416690913806363 15.722408938817239 4 28.3167534823773 19.822856729395326 34.376840593371796 17.48354919485557 5 32.79254549340085 18.418639377388963 31.75567196600202 17.033143163208166 6 27.555769609963633 18.838403841074072 37.18930027094643 16.416526278015866 7 33.629898172322875 19.307779420820214 29.27516128999534 17.787161116861572 8 27.959767185645422 28.53390825204796 26.3820541780757 17.124270384230915 9 30.804051682927174 26.008272135264356 25.36953806811766 17.81813811369081 10-11 24.71501806019434 29.96031675608453 29.119065450225406 16.205599733495724 12-13 26.121176807816532 26.301016153588463 29.04607040251807 18.531736636076932 14-15 24.095440768227235 26.593087097726904 30.153447989925663 19.158024144120205 16-17 24.186078357947164 27.342348777310697 30.977466743645643 17.494106121096493 18-19 23.859607514932048 27.53589147415896 31.2234504854062 17.38105052550279 20-21 23.331092299195383 28.671813298051486 30.57748956566899 17.419604837084144 22-23 24.759625539323878 27.643468516192303 29.874436850737183 17.722469093746632 24-25 23.018720717753112 29.122338092183657 29.83950122390902 18.019439966154213 26-27 21.63804851458163 30.708149020922313 29.54073833308787 18.113064131408187 28-29 22.209452158325853 29.77712198414246 29.361719151205495 18.65170670632619 30-31 23.416304849798546 28.337465522548623 29.580599858155715 18.665629769497116 32-33 22.061029579334864 29.229991172015662 30.542700929765882 18.16627831888359 34-35 23.948862231812267 28.168871835403387 29.838122260893947 18.044143671890396 36-37 22.81689978671395 27.92455539443018 30.498601008790853 18.759943810065018 38-39 21.42338303723543 28.838061090659362 30.97713324761321 18.761422624492003 40-41 21.477995208868037 29.89684123099665 29.574982823804618 19.05018073633069 42-43 22.607998899456287 28.524675067686996 30.321792137855297 18.54553389500142 44-45 21.710215989003057 28.983540570278027 30.75268689744047 18.55355654327845 46-47 22.31384137290463 29.381177369647293 29.58411723698268 18.720864020465388 48-49 20.96430897803684 29.622843663188885 30.0130649613454 19.399782397428876 50-51 20.744603498531163 28.27934043959683 30.772743692171268 20.20331236970074 52-53 22.916503267614022 27.986086277748974 29.10152514628838 19.995885308348623 54-55 23.970487851838783 28.111185601672535 28.613869886645837 19.30445665984285 56-57 24.134635765116855 28.314638673071617 28.509862869694075 19.040862692117454 58-59 23.43802739306575 28.296525795930076 28.88463177982442 19.380815031179754 60-61 21.618015828584998 28.663278349785966 28.538302917425142 21.1804029042039 62-63 22.378302758767504 28.487135194262407 28.67473531055214 20.459826736417945 64-65 21.329272612094062 28.844475984574675 29.732223447999196 20.094027955332066 66-67 20.65803171479689 28.280058468459192 29.655094833494484 21.406814983249443 68-69 20.228407883504804 28.120833281846654 31.78715593286498 19.863602901783562 70-71 19.843478783248276 30.27131669803606 30.405306102246033 19.47989841646963 72-73 19.87074435068076 32.117544113898475 28.371692012811017 19.640019522609755 74-75 20.087337537728207 31.82476073599699 28.358148716987188 19.72975300928762 76-77 19.903556738729183 31.813812110798057 28.46298079368025 19.81965035679251 78-79 19.70989350237101 31.91131699684218 28.592478692223168 19.78631080856365 80-81 19.548197160731902 32.02704127722548 28.414363674863502 20.010397887179117 82-83 19.30029974843343 31.59531836811776 28.535160361729684 20.56922152171913 84-85 19.445907178097624 31.256133003005317 28.842237211114334 20.455722607782725 86-87 19.87131868709701 31.01922268771479 29.11530284926669 19.99415577592151 88-89 20.144605864603164 31.130428244627318 28.63401528086752 20.090950609902 90-91 20.227591843755153 30.87076137976148 28.564539573321795 20.337107203161572 92-93 19.857199980535732 31.40265010229319 28.632317876871582 20.107832040299495 94-95 19.514741809459547 31.514682584168657 28.736881069270325 20.23369453710147 96-97 19.787609672904104 31.573847797199683 28.606350740554166 20.03219178934205 98-99 20.35512556272414 31.022182058056654 28.12000131711075 20.502691062108457 100-101 20.502710006451185 30.965332913413558 27.53757689357574 20.994380186559518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4777.0 1 3015.0 2 986.5 3 646.0 4 614.5 5 689.5 6 875.5 7 1208.5 8 1582.0 9 1981.5 10 2270.5 11 2441.5 12 2407.5 13 2242.5 14 2113.5 15 2010.5 16 1975.5 17 2047.5 18 2279.0 19 2525.0 20 2930.5 21 3599.5 22 4585.0 23 6106.0 24 8195.0 25 11265.5 26 16352.5 27 22918.5 28 30619.0 29 39864.0 30 50536.0 31 62155.5 32 79114.5 33 106930.5 34 142950.0 35 187189.5 36 247104.5 37 309076.0 38 362693.5 39 393250.5 40 399111.0 41 385477.5 42 358233.0 43 336689.5 44 309925.5 45 284045.0 46 270624.0 47 248325.5 48 215838.0 49 190116.0 50 163103.0 51 136132.5 52 112625.0 53 90323.0 54 73509.0 55 59474.0 56 45095.0 57 32375.5 58 23731.0 59 17920.5 60 13411.5 61 9875.5 62 7150.0 63 5179.5 64 3806.0 65 2826.0 66 2089.0 67 1576.5 68 1201.5 69 884.5 70 669.0 71 548.0 72 426.5 73 342.0 74 286.0 75 226.5 76 169.5 77 147.0 78 133.0 79 107.0 80 90.0 81 88.0 82 69.5 83 59.0 84 54.5 85 41.5 86 34.0 87 38.5 88 30.0 89 17.0 90 16.0 91 16.0 92 19.0 93 15.0 94 13.0 95 12.0 96 7.5 97 4.5 98 5.0 99 2.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22815078768110578 2 0.2440918223841479 3 0.22452399142591628 4 0.21735474301449523 5 0.22592410346861733 6 0.2488319607455816 7 0.22933160506651626 8 0.21635948264679206 9 0.20804315448954364 10-11 0.19389021468440884 12-13 0.2209056295806226 14-15 0.21706797307803838 16-17 0.22034052411760469 18-19 0.20886129224943517 20-21 0.20123658570364147 22-23 0.16934608188766143 24-25 0.498954386205219 26-27 0.3543632973616997 28-29 0.4872305564500716 30-31 0.3171338120816849 32-33 0.3690729082199566 34-35 0.370658577280365 36-37 0.37808085798865976 38-39 0.3720418205032745 40-41 0.3777687848225156 42-43 0.43116703387275884 44-45 0.38493803323393666 46-47 0.402911760721864 48-49 0.40071881414895877 50-51 0.4153019088587788 52-53 0.4193588600186536 54-55 0.3846090912480008 56-57 0.39816318795053457 58-59 0.39580998758960934 60-61 0.35316561115649764 62-63 0.315767437678567 64-65 0.342091230963326 66-67 0.35391627363722283 68-69 0.34446130014404286 70-71 0.3150167751978417 72-73 0.30408577997289854 74-75 0.3158686505973165 76-77 0.3227089570228017 78-79 0.33315075647378917 80-81 0.3401766199169818 82-83 0.35948298416844393 84-85 0.38895281234433243 86-87 0.3900324168109935 88-89 0.38467656652716714 90-91 0.356994833249186 92-93 0.3345846061560733 94-95 0.31138997894265225 96-97 0.3280563728967323 98-99 0.35809974094553443 100-101 0.3746058811115945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5928097.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.413736536564514 #Duplication Level Percentage of deduplicated Percentage of total 1 92.710699960236 56.00999801518179 2 3.7874280891711125 4.576253654607351 3 0.9532501030145367 1.7276820173091962 4 0.5096363146279722 1.2315613616560004 5 0.29815513300188395 0.9006332826100083 6 0.21265344277424195 0.7708313439213871 7 0.1543428431164709 0.6527099502239947 8 0.12286079575300426 0.5937983796235731 9 0.10928792661026991 0.5942242804374218 >10 0.9347471231962942 11.435109583289199 >50 0.11572175059771207 4.886787708078106 >100 0.07887924301562216 9.585688823861972 >500 0.009509889632676958 4.064263435339798 >1k 0.0027150253255965825 2.2766205426865014 >5k 5.618418022578744E-5 0.2577190563049141 >10k+ 5.617574634902573E-5 0.4361185648687313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 13329 0.2248444990019563 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 12372 0.2087010384614152 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9542 0.16096227845124667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.109162687452651E-4 0.0 0.0 2 0.001045866827077897 0.0 0.0 3 0.001062735646869476 0.0 0.0 4 0.0011133421062442129 0.0 0.0 5 0.0011976862052021078 0.0 0.0 6 0.0012314238447852658 0.0 1.6868819791578983E-5 7 0.0014675873218673715 0.0 1.6868819791578983E-5 8 0.0016531443395747403 0.0 3.3737639583157966E-5 9 0.001923045456240004 0.0 3.3737639583157966E-5 10-11 0.0032725510395663227 0.0 3.3737639583157966E-5 12-13 0.0044533684249768515 0.0 3.3737639583157966E-5 14-15 0.005693226679657907 0.0 3.3737639583157966E-5 16-17 0.00679813437600633 0.0 3.3737639583157966E-5 18-19 0.00813077113954107 0.0 5.060645937473695E-5 20-21 0.009699571380157916 0.0 5.060645937473695E-5 22-23 0.011656354475981078 0.0 5.904086927052644E-5 24-25 0.013908341918156871 0.0 6.747527916631593E-5 26-27 0.016632656314496876 0.0 6.747527916631593E-5 28-29 0.01978712561552215 0.0 6.747527916631593E-5 30-31 0.029275836748285326 0.0 6.747527916631593E-5 32-33 0.03275924803524639 0.0 6.747527916631593E-5 34-35 0.035829373237313764 0.0 7.590968906210542E-5 36-37 0.03932121893417061 0.0 8.434409895789492E-5 38-39 0.04352998947216957 0.0 9.277850885368441E-5 40-41 0.047764063239855895 8.434409895789492E-6 1.012129187494739E-4 42-43 0.05215839079556222 1.6868819791578983E-5 1.012129187494739E-4 44-45 0.057185299093452754 3.3737639583157966E-5 1.012129187494739E-4 46-47 0.06267609993561171 3.3737639583157966E-5 1.012129187494739E-4 48-49 0.06874044065068435 3.3737639583157966E-5 1.012129187494739E-4 50-51 0.07482165018554858 3.3737639583157966E-5 1.012129187494739E-4 52-53 0.08101250704905807 3.3737639583157966E-5 1.0964732864526339E-4 54-55 0.08721179832246334 3.3737639583157966E-5 1.2651614843684237E-4 56-57 0.0947099887198202 3.3737639583157966E-5 1.4338496822842136E-4 58-59 0.1028238910395697 3.3737639583157966E-5 1.5181937812421085E-4 60-61 0.11146072677285815 3.3737639583157966E-5 1.5181937812421085E-4 62-63 0.12074701206812236 3.3737639583157966E-5 1.5181937812421085E-4 64-65 0.13076709102432027 3.3737639583157966E-5 1.6025378802000034E-4 66-67 0.14115828401593294 3.3737639583157966E-5 1.6868819791578983E-4 68-69 0.1527302943929561 3.3737639583157966E-5 1.6868819791578983E-4 70-71 0.16538190923664037 3.3737639583157966E-5 1.6868819791578983E-4 72-73 0.17962762755062883 3.3737639583157966E-5 1.6868819791578983E-4 74-75 0.1957626536812741 3.3737639583157966E-5 1.8555701770736881E-4 76-77 0.21353395533170255 3.3737639583157966E-5 1.8555701770736881E-4 78-79 0.23441755423367736 3.3737639583157966E-5 1.8555701770736881E-4 80-81 0.2578230416944932 3.3737639583157966E-5 1.8555701770736881E-4 82-83 0.28373354889435853 3.3737639583157966E-5 1.939914276031583E-4 84-85 0.3125454930983754 3.3737639583157966E-5 2.024258374989478E-4 86-87 0.3465024273388239 3.3737639583157966E-5 2.024258374989478E-4 88-89 0.38508985261206086 3.3737639583157966E-5 2.108602473947373E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2775 0.0 81.00937 9 AGAGCGG 3075 0.0 75.11104 8 CGGGAGA 3860 0.0 72.746666 4 GAGAGCG 3650 0.0 69.646484 7 CGGAAAG 2155 0.0 67.35236 5 GATCGGG 5170 0.0 66.43324 1 AAGCGGC 1085 0.0 63.817974 9 TCGGGAG 4490 0.0 63.067654 3 ATCGGGA 5290 0.0 62.49863 2 GATCCGG 980 0.0 61.96142 1 GTTCGGA 735 0.0 59.373665 2 TCGGAAA 1835 0.0 57.12614 4 TCGGGAA 1665 0.0 56.691444 3 GGATCGG 2155 0.0 54.373478 1 AAGGGGC 3755 0.0 52.794205 9 GGTTCGG 1065 0.0 52.561756 1 GTCGGGA 875 0.0 51.500202 2 AGAGCGT 5985 0.0 51.348854 8 GGACCGG 905 0.0 49.798065 1 GAGCGTC 6185 0.0 49.45838 9 >>END_MODULE