FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5928097
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC133290.2248444990019563No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG123720.2087010384614152No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA95420.16096227845124667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC27750.081.009379
AGAGCGG30750.075.111048
CGGGAGA38600.072.7466664
GAGAGCG36500.069.6464847
CGGAAAG21550.067.352365
GATCGGG51700.066.433241
AAGCGGC10850.063.8179749
TCGGGAG44900.063.0676543
ATCGGGA52900.062.498632
GATCCGG9800.061.961421
GTTCGGA7350.059.3736652
TCGGAAA18350.057.126144
TCGGGAA16650.056.6914443
GGATCGG21550.054.3734781
AAGGGGC37550.052.7942059
GGTTCGG10650.052.5617561
GTCGGGA8750.051.5002022
AGAGCGT59850.051.3488548
GGACCGG9050.049.7980651
GAGCGTC61850.049.458389
Produced by FastQC (version 0.11.2)