##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6731577 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11856107417326 33.0 33.0 33.0 33.0 33.0 2 32.06510940898396 33.0 33.0 33.0 33.0 33.0 3 32.05113838852323 33.0 33.0 33.0 33.0 33.0 4 35.78502347963932 37.0 37.0 37.0 33.0 37.0 5 35.76400715612404 37.0 37.0 37.0 33.0 37.0 6 35.80398619818209 37.0 37.0 37.0 33.0 37.0 7 35.7408501752264 37.0 37.0 37.0 33.0 37.0 8 35.70268185300413 37.0 37.0 37.0 33.0 37.0 9 35.5991330114771 37.0 37.0 37.0 33.0 37.0 10-11 35.55780257731584 37.0 37.0 37.0 33.0 37.0 12-13 35.600321291727035 37.0 37.0 37.0 33.0 37.0 14-15 37.41897596952393 40.0 37.0 40.0 33.0 40.0 16-17 37.37771988643969 40.0 37.0 40.0 33.0 40.0 18-19 37.368648757935915 40.0 37.0 40.0 33.0 40.0 20-21 37.3275028273464 40.0 37.0 40.0 33.0 40.0 22-23 37.40949030219814 40.0 37.0 40.0 33.0 40.0 24-25 37.19139311635297 40.0 37.0 40.0 33.0 40.0 26-27 37.11674753181907 40.0 37.0 40.0 33.0 40.0 28-29 36.983418595672305 40.0 37.0 40.0 33.0 40.0 30-31 37.02166936514282 40.0 37.0 40.0 33.0 40.0 32-33 36.93963309043335 40.0 37.0 40.0 33.0 40.0 34-35 36.87713815054036 40.0 37.0 40.0 33.0 40.0 36-37 36.80080871094545 40.0 37.0 40.0 33.0 40.0 38-39 36.74987584632843 40.0 37.0 40.0 33.0 40.0 40-41 36.64483812634097 40.0 37.0 40.0 33.0 40.0 42-43 36.56399020615823 40.0 37.0 40.0 33.0 40.0 44-45 36.48978805412165 40.0 37.0 40.0 33.0 40.0 46-47 36.381872628063235 40.0 37.0 40.0 33.0 40.0 48-49 36.25834919514402 38.5 37.0 40.0 33.0 40.0 50-51 35.13476292405183 37.0 35.0 38.5 30.0 38.5 52-53 35.563113665638824 37.0 37.0 38.5 33.0 40.0 54-55 36.351914640506976 37.0 37.0 40.0 33.0 40.0 56-57 36.415958548791764 38.5 37.0 40.0 33.0 40.0 58-59 36.25266523728392 37.0 37.0 40.0 33.0 40.0 60-61 36.05015578073311 37.0 37.0 40.0 33.0 40.0 62-63 35.82202736446452 37.0 37.0 40.0 30.0 40.0 64-65 35.60089909095595 37.0 37.0 40.0 27.0 40.0 66-67 35.36895143589682 37.0 37.0 40.0 27.0 40.0 68-69 35.182629345248515 37.0 35.0 40.0 27.0 40.0 70-71 34.89799255063115 37.0 33.0 37.0 27.0 40.0 72-73 34.68478953742934 37.0 33.0 37.0 27.0 40.0 74-75 34.438703664832175 37.0 33.0 37.0 27.0 40.0 76-77 34.18775897534857 37.0 33.0 37.0 27.0 37.0 78-79 33.90395764618009 37.0 33.0 37.0 27.0 37.0 80-81 33.62644050569428 37.0 33.0 37.0 27.0 37.0 82-83 33.39254234186134 37.0 33.0 37.0 27.0 37.0 84-85 33.2083223589361 37.0 33.0 37.0 27.0 37.0 86-87 32.99615491288297 37.0 33.0 37.0 27.0 37.0 88-89 32.86239071468691 37.0 33.0 37.0 27.0 37.0 90-91 32.73618952884294 37.0 33.0 37.0 27.0 37.0 92-93 32.57293149881521 35.0 33.0 37.0 27.0 37.0 94-95 32.4441946515653 33.0 33.0 37.0 22.0 37.0 96-97 32.28166275153653 33.0 33.0 37.0 22.0 37.0 98-99 32.163812580618185 33.0 33.0 37.0 22.0 37.0 100-101 30.871187241860266 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1647893522741697 2306 2 0.16112618574626225 2306 3 0.17520275235064986 2306 4 0.21558274140883782 2306 5 0.14952936130024597 2306 6 0.14042619584485294 2306 7 0.23215085893323817 2306 8 0.2307970827097776 2306 9 0.22022766697693896 2306 10-11 0.2657914351697386 2306 12-13 0.21741765373020883 2306 14-15 0.3747823251185096 2306 16-17 0.4643863852726682 2306 18-19 0.4130678072276126 2306 20-21 0.42497137932672757 2306 22-23 0.384902807831665 2306 24-25 0.34751477329641745 2306 26-27 0.243517180952864 2306 28-29 0.3450102269313504 2306 30-31 0.4457189471666112 2306 32-33 0.5562528176268628 2306 34-35 0.5202801150938043 2306 36-37 0.5962008445437519 2306 38-39 0.4389153952030682 2306 40-41 0.4553016674128756 2306 42-43 0.4533642074711395 2306 44-45 0.49256008279407837 2306 46-47 0.4128674846529634 2306 48-49 0.5192780663226486 2306 50-51 0.3665648546160156 2306 52-53 0.4794232895291586 2306 54-55 0.5329185627578994 2306 56-57 0.580890729102272 2306 58-59 0.48163048324546054 2306 60-61 0.40877539460332457 2306 62-63 0.4619467003595048 2306 64-65 0.443346120020621 2306 66-67 0.43512128101502157 2306 68-69 0.4540885476728036 2306 70-71 0.4958543900940171 2306 72-73 0.44932409195730116 2306 74-75 0.42560400776843466 2306 76-77 0.40858489259462516 2306 78-79 0.4485534882320863 2306 80-81 0.3181003652174539 2306 82-83 0.3751398446546048 2306 84-85 0.3756542899709956 2306 86-87 0.39555451178785717 2306 88-89 0.397198368868132 2306 90-91 0.44322631810379676 2306 92-93 0.11071117561287735 2306 94-95 0.2096514327597987 2306 96-97 0.29511973170483685 2306 98-99 0.2465403903527914 2306 100-101 0.035428373467478735 2307 1 0.1536849488472143 2307 2 0.14970454239718833 2307 3 0.1264521423706242 2307 4 0.19820779360301088 2307 5 0.22243578407552178 2307 6 0.2633989743856304 2307 7 0.22864929688128655 2307 8 0.24280491057718478 2307 9 0.283913556189475 2307 10-11 0.24245764906772393 2307 12-13 0.17849554503553833 2307 14-15 0.31465099704735877 2307 16-17 0.3042483411327481 2307 18-19 0.32409453567205304 2307 20-21 0.32751855530785434 2307 22-23 0.3199122153586913 2307 24-25 0.2557590621225856 2307 26-27 0.30542031863981833 2307 28-29 0.19587096534675652 2307 30-31 0.39585035069920593 2307 32-33 0.4106239219296768 2307 34-35 0.4299174222269144 2307 36-37 0.48006738845979413 2307 38-39 0.4014492145081192 2307 40-41 0.4049100979657041 2307 42-43 0.4114088253092234 2307 44-45 0.20097722864093015 2307 46-47 0.2600070682063915 2307 48-49 0.33837325842232957 2307 50-51 0.09733005883984447 2307 52-53 0.22938402442303385 2307 54-55 0.35012754568852245 2307 56-57 0.34901815330124464 2307 58-59 0.3897221890263083 2307 60-61 0.48691396194259795 2307 62-63 0.4241070609646016 2307 64-65 0.3824663904744412 2307 66-67 0.2922496711205156 2307 68-69 0.21503146360372227 2307 70-71 0.2820726145364816 2307 72-73 0.17497152601657007 2307 74-75 0.22245457398081925 2307 76-77 0.1768018392232662 2307 78-79 0.15523222873023457 2307 80-81 0.2716098004136782 2307 82-83 0.10396289762351074 2307 84-85 0.15907571196649428 2307 86-87 0.11623899741749 2307 88-89 0.05053434335209772 2307 90-91 0.07178558300869042 2307 92-93 0.04161190859506547 2307 94-95 0.13149945843680655 2307 96-97 0.12883422080568607 2307 98-99 0.012242483653324143 2307 100-101 -0.1688376130400151 2201 1 0.10090947070078471 2201 2 0.039587579608756585 2201 3 0.06987590428448698 2201 4 0.1481016567714022 2201 5 0.14858312322515133 2201 6 0.1357535138579138 2201 7 0.16090056521642992 2201 8 0.09632812270226054 2201 9 0.19466044107346647 2201 10-11 0.15531267676943372 2201 12-13 0.04660593795409085 2201 14-15 0.20821141013190925 2201 16-17 0.259579063500027 2201 18-19 0.2248027336809244 2201 20-21 0.24193246772373556 2201 22-23 0.11104501376239284 2201 24-25 0.040418575166803805 2201 26-27 0.12988883845556387 2201 28-29 0.19103165650980714 2201 30-31 0.3088364573879545 2201 32-33 0.4044687817229189 2201 34-35 0.4514417063339877 2201 36-37 0.38869074068549025 2201 38-39 0.2876169319294206 2201 40-41 0.30900273419233315 2201 42-43 0.3997900263536138 2201 44-45 0.36852323895742956 2201 46-47 0.36103022726305056 2201 48-49 0.3485003042097645 2201 50-51 0.2795967170155649 2201 52-53 0.28551156905357544 2201 54-55 0.41099436519222365 2201 56-57 0.4763982717499502 2201 58-59 0.4907740050274967 2201 60-61 0.4421756953693361 2201 62-63 0.37475980247896246 2201 64-65 0.4416957840085942 2201 66-67 0.32515288494377614 2201 68-69 0.3290879602391783 2201 70-71 0.371478832032075 2201 72-73 0.3307501458132194 2201 74-75 0.24786797693991502 2201 76-77 0.19015040319192877 2201 78-79 0.16415913229430856 2201 80-81 0.1934153471244997 2201 82-83 0.2066818579071068 2201 84-85 0.2295017452085517 2201 86-87 0.25576939500606244 2201 88-89 0.2918576573685243 2201 90-91 0.18598854847176938 2201 92-93 0.04911100643199262 2201 94-95 0.12208246805280965 2201 96-97 0.14632185586480517 2201 98-99 0.1660827795628137 2201 100-101 -0.006795062784227923 1101 1 0.17364836447051601 1101 2 0.11267231560191249 1101 3 0.10599061791875641 1101 4 0.19226556925100624 1101 5 0.19959194592058083 1101 6 0.25512369984107863 1101 7 0.28320029979181527 1101 8 0.2370176921193945 1101 9 0.24158214313782622 1101 10-11 0.26968668607556623 1101 12-13 0.2539070321914494 1101 14-15 0.3840501588710268 1101 16-17 0.28597033470261835 1101 18-19 0.29838072482104394 1101 20-21 0.2568289009933906 1101 22-23 0.21210020441721866 1101 24-25 0.05982943479855862 1101 26-27 0.2272329073079149 1101 28-29 0.10897803237151038 1101 30-31 0.2632525116256801 1101 32-33 0.35146758940395983 1101 34-35 0.3250183029490117 1101 36-37 0.40053717325787375 1101 38-39 0.2762641031352686 1101 40-41 0.28147794650877955 1101 42-43 0.24759353311938526 1101 44-45 0.11136397954322064 1101 46-47 0.2319661162960358 1101 48-49 0.18811550825202517 1101 50-51 0.29936027528579245 1101 52-53 0.3419563086724864 1101 54-55 0.4564843548262516 1101 56-57 0.3705253455249746 1101 58-59 0.5054363905021333 1101 60-61 0.46983830861639575 1101 62-63 0.4381007592161481 1101 64-65 0.39668998510840225 1101 66-67 0.4431071050091262 1101 68-69 0.41141136687805613 1101 70-71 0.36611914914708166 1101 72-73 0.29143046488055546 1101 74-75 0.3004742281310726 1101 76-77 0.3032602754999161 1101 78-79 0.2901562758420724 1101 80-81 0.3614979797089717 1101 82-83 0.3061813977138286 1101 84-85 0.22132936331320963 1101 86-87 0.25629739706995025 1101 88-89 0.21217507452212203 1101 90-91 0.25267316964902165 1101 92-93 0.1855492498881901 1101 94-95 0.24411503486218322 1101 96-97 0.3252656890424177 1101 98-99 0.2677289583316309 1101 100-101 0.1350072795530899 2304 1 0.07073083173445127 2304 2 0.0734741572051476 2304 3 0.05391552560388391 2304 4 0.11696356266417496 2304 5 0.14995927068658688 2304 6 0.09279059324421723 2304 7 0.2122290697564324 2304 8 0.0711950157094492 2304 9 0.11633584471895375 2304 10-11 0.15905942605625967 2304 12-13 0.09046912654089567 2304 14-15 0.13492930393363878 2304 16-17 0.21778617973996006 2304 18-19 0.1504509339512481 2304 20-21 0.2055724895368698 2304 22-23 0.06774610060737984 2304 24-25 0.07098656674646975 2304 26-27 0.08693834164984082 2304 28-29 0.0455825083652428 2304 30-31 0.12058025427958086 2304 32-33 0.14807059391305444 2304 34-35 0.22491064608108502 2304 36-37 0.23839868957709598 2304 38-39 0.11669216083533485 2304 40-41 0.1712259288187994 2304 42-43 0.0996608040913145 2304 44-45 0.1224950460754286 2304 46-47 0.15087004443149965 2304 48-49 0.1028931281596428 2304 50-51 0.08628292460970499 2304 52-53 0.10248472629866257 2304 54-55 0.2640859510125182 2304 56-57 0.35893391598486346 2304 58-59 0.3126647546778756 2304 60-61 0.30847668428979347 2304 62-63 0.22810155028518864 2304 64-65 0.16448768119369817 2304 66-67 0.18161528075236788 2304 68-69 0.16099578071479215 2304 70-71 0.2208115012963816 2304 72-73 0.23868181267045685 2304 74-75 0.27271170815760826 2304 76-77 0.20433930810838064 2304 78-79 0.10134696121370723 2304 80-81 0.15337817831609613 2304 82-83 0.19415437160628102 2304 84-85 0.13272665791206606 2304 86-87 0.17047900040539332 2304 88-89 0.05231527862342489 2304 90-91 -0.009444877508727245 2304 92-93 0.09655851859194087 2304 94-95 0.13199586055132073 2304 96-97 0.17804119361353798 2304 98-99 0.0856629574688128 2304 100-101 -0.0018593660688033253 2202 1 0.10625631264355917 2202 2 0.08701801893646888 2202 3 0.07802765135824785 2202 4 0.14888920761826796 2202 5 0.1384794254193551 2202 6 0.12122932131418906 2202 7 0.19968514243595337 2202 8 0.12073123065739111 2202 9 0.16976308267283002 2202 10-11 0.1592751435260169 2202 12-13 0.1270716031203989 2202 14-15 0.17891569670509 2202 16-17 0.18571719363549022 2202 18-19 0.11277653705320745 2202 20-21 0.1723690872131911 2202 22-23 0.12596420042927292 2202 24-25 0.01594410557201087 2202 26-27 0.12242721395590905 2202 28-29 0.04215367272664139 2202 30-31 0.15545476875760755 2202 32-33 0.16484391104049934 2202 34-35 0.29144721756498626 2202 36-37 0.3734414895087639 2202 38-39 0.24776504456977477 2202 40-41 0.20152262608011995 2202 42-43 0.18139673299363324 2202 44-45 0.22286979924515293 2202 46-47 0.20230366015060497 2202 48-49 0.12204612461133735 2202 50-51 0.16195197611112633 2202 52-53 0.10196367686246077 2202 54-55 0.20300565305993956 2202 56-57 0.32741832342281896 2202 58-59 0.32537532772293787 2202 60-61 0.22320392587149485 2202 62-63 0.2155104131829404 2202 64-65 0.3895765328860321 2202 66-67 0.39429913161943375 2202 68-69 0.36267810068756035 2202 70-71 0.24758791649068002 2202 72-73 0.2322885511039985 2202 74-75 0.2139683544660116 2202 76-77 0.17394176450660837 2202 78-79 0.17896017279305454 2202 80-81 0.17550662231773373 2202 82-83 0.22546791110741538 2202 84-85 0.20590893716188674 2202 86-87 0.21706030946416632 2202 88-89 0.17811979697194147 2202 90-91 0.1219111447896637 2202 92-93 0.14553899752736044 2202 94-95 0.2834213857391177 2202 96-97 0.23214864884293007 2202 98-99 0.13708266852572137 2202 100-101 0.12770598800576138 1102 1 -0.16196397236228322 1102 2 -0.19441745299257107 1102 3 -0.19191097777854793 1102 4 -0.11389198111531584 1102 5 -0.1347011248869805 1102 6 -0.10271740596120083 1102 7 -0.11154031714747958 1102 8 -0.07690718805508823 1102 9 -0.030132674523358105 1102 10-11 -0.09176816694347423 1102 12-13 -0.09415075557283359 1102 14-15 -0.13639688208812828 1102 16-17 -0.14822709391470568 1102 18-19 -0.1700992340996237 1102 20-21 -0.15047687409069255 1102 22-23 -0.12023169059091998 1102 24-25 -0.19120760824669247 1102 26-27 -0.14211611121199041 1102 28-29 -0.20210161999139586 1102 30-31 -0.025057948632650096 1102 32-33 -0.02354266249479764 1102 34-35 0.05644471830859743 1102 36-37 -0.011288027625838026 1102 38-39 -0.016239681363899194 1102 40-41 -0.016773050174720083 1102 42-43 -0.004423427175836991 1102 44-45 -0.03954280975619895 1102 46-47 0.051917123598400394 1102 48-49 0.006318515951896586 1102 50-51 0.21314969626669722 1102 52-53 0.14532522200980935 1102 54-55 0.2088344547349834 1102 56-57 0.268883989782843 1102 58-59 0.26786528618070804 1102 60-61 0.25430154511905556 1102 62-63 0.24554634894951732 1102 64-65 0.29009061369862366 1102 66-67 0.2596364421982926 1102 68-69 0.2937882768018909 1102 70-71 0.2379649891639346 1102 72-73 0.2449116660857058 1102 74-75 0.241788320658614 1102 76-77 0.21313066045377838 1102 78-79 0.22727714962424983 1102 80-81 0.1873384070849724 1102 82-83 0.21716078115672133 1102 84-85 0.1297270164791371 1102 86-87 -0.03277119882547197 1102 88-89 0.05034792197226068 1102 90-91 0.020739004607513323 1102 92-93 0.1548957335027339 1102 94-95 0.1288971811469537 1102 96-97 0.19338844332650496 1102 98-99 0.057819956008593465 1102 100-101 -0.07245856832407682 2305 1 -0.0869715022999955 2305 2 -0.047867865588983705 2305 3 -0.09500162511776011 2305 4 -0.09353103893454318 2305 5 -0.012265148271971782 2305 6 -0.022125091744364056 2305 7 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-0.6777909718595865 1213 88-89 -0.614811048824464 1213 90-91 -0.5108231347446477 1213 92-93 -0.5205121171259393 1213 94-95 -0.536236152666909 1213 96-97 -0.49829854313504995 1213 98-99 -0.471040608666474 1213 100-101 -0.3271463050270178 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7358.0 3 629.0 4 591.0 5 521.0 6 1016.0 7 3188.0 8 13089.0 9 25440.0 10 32860.0 11 22483.0 12 15200.0 13 12401.0 14 11424.0 15 11721.0 16 12572.0 17 12701.0 18 13795.0 19 15358.0 20 17401.0 21 20050.0 22 22495.0 23 24650.0 24 28913.0 25 33537.0 26 39713.0 27 48805.0 28 59950.0 29 74526.0 30 93422.0 31 121483.0 32 166728.0 33 239670.0 34 365541.0 35 616645.0 36 1195977.0 37 2303250.0 38 1045569.0 39 905.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.418576311646916 26.76725172530654 21.465868799870396 16.348303163176148 2 31.963326356586148 24.47262612131081 29.94694018454474 13.617107337558302 3 36.1233099078715 21.28403077164061 25.290803200808092 17.3018561196798 4 30.924748043748103 20.934221667307128 30.19535496162237 17.945675327322405 5 29.78269832636064 19.638211228977884 32.60086453722273 17.978225907438738 6 26.47097721606462 19.779056294103103 35.45262555887513 18.29734093095714 7 32.19511824673699 19.748569798267066 27.977528943167325 20.07878301182862 8 29.858289845289214 27.393589342936608 23.634885344468337 19.113235467305838 9 27.537195466722007 27.052729146376358 25.551735113550794 19.858340273350837 10-11 23.359334367956492 29.484229538679735 29.932766364605527 17.22366972875825 12-13 23.62198827358382 27.453464256823967 30.184786568892434 18.739760900699775 14-15 22.47226735776089 27.68923420439981 31.131474234677487 18.70702420316181 16-17 22.141779380116475 27.972286502050085 32.0184018150486 17.867532302784838 18-19 21.929877320295933 28.10551005384032 31.889070928832968 18.07554169703078 20-21 21.787728666422236 28.813275473305833 31.183343850496403 18.21565200977553 22-23 22.311414562658335 28.75376941703652 30.474642969389894 18.460173050915255 24-25 21.764837187912704 29.0405296348085 30.50489403048476 18.68973914679404 26-27 21.45947180164313 29.510947298317653 30.355165958479645 18.67441494155957 28-29 21.677113827649986 29.403651159225063 30.107388265489615 18.811846747635336 30-31 21.90714633659794 29.107366850057776 30.131764840189962 18.853721973154318 32-33 21.367505245716643 29.408944319655305 30.35769137173552 18.865859062892536 34-35 22.070267285163002 28.99084132455557 30.224717501076793 18.714173889204638 36-37 21.53156858467225 28.768420991411382 30.69043309657572 19.00957732734065 38-39 21.278919395682905 29.20915161571356 30.531806349228834 18.980122639374702 40-41 21.35686192871691 29.65780800157952 29.7911845610071 19.194145508696465 42-43 21.52576790137309 29.060473387145446 30.183606102910467 19.230152608571 44-45 21.11450628266645 29.097282228884307 30.498925174422403 19.28928631402684 46-47 21.37127948366418 29.346373192518087 29.889906174051973 19.39244114976576 48-49 21.075049758392026 29.61762240989549 29.723661277936163 19.583666553776315 50-51 20.997115257092997 29.22405532762949 29.90219443050831 19.876634984769208 52-53 21.634635001635495 29.090020733648146 29.4028642197804 19.87248004493596 54-55 21.828538021900012 29.244774335324685 29.24031514123826 19.686372501537043 56-57 21.820331979512734 29.187441395408463 29.368808459629886 19.623418165448914 58-59 21.66164284068815 29.229345169471816 29.50266695691725 19.606345032922786 60-61 20.91469689058995 29.292475854445936 29.414116789013285 20.378710465950824 62-63 21.021399664097785 29.205221439649264 29.473992392088306 20.299386504164648 64-65 20.787406516504735 29.299897915009478 29.684907998287454 20.227787570198338 66-67 20.639795508751373 29.040016012219382 29.705961830834777 20.61422664819447 68-69 20.46221163855683 29.12842084361268 30.294925589938387 20.114441927892106 70-71 20.293205029586748 29.96658949097651 29.690770414877644 20.049435064559102 72-73 20.29417065671132 30.401141330469873 29.200287090986432 20.104400921832376 74-75 20.455244890833 30.417339982762908 29.079967671885438 20.047447454518654 76-77 20.462970470333214 30.335521985687063 29.113545121989027 20.087962421990696 78-79 20.328791499438683 30.30458367209957 29.349328093235428 20.01729673522632 80-81 20.15571361735015 30.60539469415132 29.236660116797513 20.002231571701017 82-83 20.091582908251223 30.40121663252964 29.25890901907139 20.248291440147757 84-85 20.207192336017787 30.032217623018916 29.480183969458135 20.280406071505165 86-87 20.357873134384008 30.01405690473669 29.524548501168553 20.10352145971075 88-89 20.581469378880033 30.283127300504393 29.095906341871974 20.039496978743603 90-91 20.615530566343914 30.104094767932786 29.07632694420948 20.204047721513827 92-93 20.400402076026413 30.247493490337856 29.018235550458016 20.333868883177715 94-95 20.131383508793004 30.300275145042406 29.00208769262798 20.566253653536613 96-97 20.25131688686388 30.599374774513333 28.819046430895828 20.33026190772696 98-99 20.53458274613973 30.519264522493067 28.687794503808234 20.25835822755897 100-101 20.664207082272902 30.351374311792807 28.34990130496356 20.634517300970725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5436.0 1 3377.5 2 996.5 3 552.0 4 445.5 5 509.0 6 671.5 7 961.0 8 1381.0 9 1850.5 10 2192.0 11 2301.0 12 2205.0 13 1974.0 14 1664.0 15 1474.5 16 1393.5 17 1333.5 18 1387.5 19 1581.0 20 1861.5 21 2358.0 22 3281.5 23 4773.5 24 7009.5 25 10672.5 26 17005.5 27 25872.5 28 37151.0 29 50721.0 30 66837.0 31 85727.5 32 110224.5 33 143671.5 34 184424.5 35 230718.0 36 283539.0 37 341039.5 38 394379.5 39 427211.0 40 442546.0 41 437131.0 42 411771.0 43 389261.0 44 361940.0 45 334407.5 46 318812.0 47 292991.5 48 252697.5 49 215101.0 50 176904.5 51 142678.5 52 116223.0 53 92653.0 54 75647.0 55 60032.0 56 43213.5 57 30052.0 58 21527.0 59 15823.5 60 11505.0 61 8202.0 62 5835.0 63 4051.0 64 2861.0 65 2031.5 66 1448.5 67 1036.0 68 762.0 69 574.0 70 446.5 71 350.5 72 260.0 73 225.0 74 199.5 75 147.5 76 118.5 77 105.5 78 99.0 79 85.0 80 58.5 81 46.0 82 42.0 83 36.0 84 33.0 85 26.5 86 18.0 87 18.0 88 15.0 89 14.0 90 18.5 91 15.5 92 9.0 93 8.0 94 6.0 95 4.5 96 2.5 97 1.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2327686365319746 2 0.25092188650594055 3 0.23059975396552695 4 0.2233354829039317 5 0.2319515917295457 6 0.25546762667945416 7 0.23643791046288262 8 0.22253329346154696 9 0.21532844384012842 10-11 0.20071076955667297 12-13 0.22710874435514888 14-15 0.22399654642589695 16-17 0.22703446755492807 18-19 0.2152838777599959 20-21 0.20776706557764993 22-23 0.17388198931691637 24-25 0.5070431490273378 26-27 0.35937344250834535 28-29 0.49417840722909356 30-31 0.3235868801619591 32-33 0.37365687119080715 34-35 0.37602630111785096 36-37 0.3839219249813231 38-39 0.37627884223860175 40-41 0.38254037649721606 42-43 0.43720067377971017 44-45 0.3907405352415935 46-47 0.40953256569745844 48-49 0.40750480905143033 50-51 0.42159511805331795 52-53 0.4264156823876485 54-55 0.39113420228276374 56-57 0.40432576200197967 58-59 0.40147353287350057 60-61 0.35847469322567355 62-63 0.32070494031339164 64-65 0.3482244947952018 66-67 0.35949228538869865 68-69 0.34978430759983875 70-71 0.3203187009522434 72-73 0.30917718091912194 74-75 0.32129172703513603 76-77 0.3286154195369079 78-79 0.3386502152467394 80-81 0.346137316708997 82-83 0.3645431078037138 84-85 0.39442466453254565 86-87 0.3964449934985517 88-89 0.39001262259942954 90-91 0.36204740731629453 92-93 0.3401208958911114 94-95 0.3163894582205626 96-97 0.3339113553926517 98-99 0.36444654796342674 100-101 0.37979213488904606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6731577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.60945761132349 #Duplication Level Percentage of deduplicated Percentage of total 1 91.96536977503345 62.17728769514815 2 4.771559411460949 6.452050875381614 3 1.107125451604469 2.245564538720092 4 0.5283134777159646 1.4287595070851362 5 0.22772036101208135 0.7698025047540799 6 0.23207921031014347 0.9414449719159846 7 0.15768629488678224 0.7462759409024192 8 0.099584140566587 0.5386263784317394 9 0.08454726306327807 0.5144575138407105 >10 0.6736809930789447 9.11600062354366 >50 0.08410829253289377 3.972289271181459 >100 0.06242467704936984 8.104799667107967 >500 0.005045353163354393 2.286384737990266 >1k 7.552985217728617E-4 0.706255773996621 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.982019220756147E-4 0.0 0.0 2 8.170448024289108E-4 0.0 0.0 3 9.50743042826369E-4 0.0 0.0 4 9.95309122958855E-4 0.0 0.0 5 0.001158718083444637 0.0 0.0 6 0.0014706806443720395 0.0 0.0 7 0.0017677878452552797 0.0 0.0 8 0.00221344864658014 0.0 0.0 9 0.0024511344072867323 0.0 1.4855360044162015E-5 10-11 0.004553167853535657 0.0 1.4855360044162015E-5 12-13 0.0068260379402924455 0.0 2.2283040066243024E-5 14-15 0.009693122428815714 0.0 3.7138400110405036E-5 16-17 0.013510949960165351 0.0 5.199376015456705E-5 18-19 0.01684597829007972 0.0 7.427680022081007E-5 20-21 0.01834636965454009 0.0 8.170448024289107E-5 22-23 0.02012158517981745 0.0 1.039875203091341E-4 24-25 0.022156769505867646 0.0 1.1141520033121511E-4 26-27 0.024377645832469867 0.0 1.1884288035329612E-4 28-29 0.02735614552132435 0.0 1.1884288035329612E-4 30-31 0.046125892937123054 0.0 1.2627056037537712E-4 32-33 0.05095388495147571 0.0 1.4112592041953912E-4 34-35 0.054467177601920026 0.0 1.4855360044162014E-4 36-37 0.05861925073426331 0.0 1.4855360044162014E-4 38-39 0.06335811058835099 0.0 1.4855360044162014E-4 40-41 0.06852777588371937 0.0 1.4855360044162014E-4 42-43 0.0746184735018258 0.0 1.4855360044162014E-4 44-45 0.08137766232191951 0.0 1.5598128046370117E-4 46-47 0.0883076877825211 0.0 1.7083664050786317E-4 48-49 0.09613646252579447 0.0 1.7826432052994417E-4 50-51 0.10382411134864832 0.0 1.7826432052994417E-4 52-53 0.11297501313585212 0.0 1.8569200055202517E-4 54-55 0.12328463300650055 0.0 1.931196805741062E-4 56-57 0.1342553163991142 0.0 2.005473605961872E-4 58-59 0.14655555451568036 0.0 2.3768576070659224E-4 60-61 0.15941286863390258 0.0 2.3768576070659224E-4 62-63 0.1746544680392128 0.0 2.5254112075075424E-4 64-65 0.1915153016893367 0.0 2.971072008832403E-4 66-67 0.21015877854476 0.0 3.119625609274023E-4 68-69 0.229686149322811 0.0 3.119625609274023E-4 70-71 0.2525634037908205 0.0 3.119625609274023E-4 72-73 0.2798229894718578 0.0 3.2681792097156434E-4 74-75 0.3099273766013521 0.0 3.2681792097156434E-4 76-77 0.3440501386227922 0.0 3.4910096103780736E-4 78-79 0.3815970611344117 0.0 3.5652864105988833E-4 80-81 0.42367486845950064 0.0 3.5652864105988833E-4 82-83 0.4704246865184785 0.0 3.6395632108196936E-4 84-85 0.5251221222010831 0.0 3.713840011040504E-4 86-87 0.585724563501242 0.0 3.713840011040504E-4 88-89 0.6553590637082514 0.0 3.713840011040504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1380 0.0 55.65716 9 AGAGCGG 1545 0.0 52.7823 8 CGGGAGA 2005 0.0 51.086784 4 GAGAGCG 1905 0.0 47.788177 7 GATCGGG 2625 0.0 46.97515 1 ATCGGGA 2815 0.0 39.7569 2 TTGTGTT 53730 0.0 39.350243 1 AAGCGGC 665 0.0 35.64786 9 GGCGCCG 1285 0.0 35.49028 56-57 GATCCGG 550 0.0 35.354553 1 TCGGGAG 2900 0.0 35.156826 3 CGCCGGA 730 0.0 35.137962 58-59 TGTGTTT 95255 0.0 35.051697 1 AGCGGCG 1335 0.0 34.626488 10-11 GGTCCGG 390 0.0 34.050037 1 ATTCGGA 650 0.0 33.560112 2 CGCCGTA 1205 0.0 33.11295 58-59 GGTTCGG 610 0.0 32.654545 1 GGGCGCC 2060 0.0 31.709644 54-55 CGCGCGG 60 0.003982237 31.617891 1 >>END_MODULE