##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2365610 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78448603108712 33.0 33.0 33.0 27.0 33.0 2 31.709720114473644 33.0 33.0 33.0 27.0 33.0 3 31.67243290314126 33.0 33.0 33.0 27.0 33.0 4 35.346944762661636 37.0 37.0 37.0 33.0 37.0 5 35.326585531850135 37.0 37.0 37.0 33.0 37.0 6 35.36358402272564 37.0 37.0 37.0 33.0 37.0 7 35.291735746805266 37.0 37.0 37.0 33.0 37.0 8 35.257558092838636 37.0 37.0 37.0 33.0 37.0 9 35.126537764043945 37.0 37.0 37.0 33.0 37.0 10-11 35.0748470796116 37.0 37.0 37.0 33.0 37.0 12-13 35.09354606211505 37.0 37.0 37.0 33.0 37.0 14-15 36.751518635785274 40.0 37.0 40.0 33.0 40.0 16-17 36.69016828640393 40.0 37.0 40.0 33.0 40.0 18-19 36.71043388386082 40.0 37.0 40.0 33.0 40.0 20-21 36.67704735776396 40.0 37.0 40.0 33.0 40.0 22-23 36.77677343264528 40.0 37.0 40.0 33.0 40.0 24-25 36.51451528358436 40.0 37.0 40.0 33.0 40.0 26-27 36.400744839597394 40.0 37.0 40.0 33.0 40.0 28-29 36.28320496616095 40.0 37.0 40.0 33.0 40.0 30-31 36.33834972797714 40.0 37.0 40.0 33.0 40.0 32-33 36.25750969094652 40.0 37.0 40.0 33.0 40.0 34-35 36.1558179497043 40.0 37.0 40.0 33.0 40.0 36-37 36.084612425547746 40.0 37.0 40.0 33.0 40.0 38-39 36.024394976348596 38.5 37.0 40.0 30.0 40.0 40-41 35.92649971888857 37.0 37.0 40.0 27.0 40.0 42-43 35.7881675339553 37.0 37.0 40.0 27.0 40.0 44-45 35.69509936126411 37.0 37.0 40.0 27.0 40.0 46-47 35.6048355392478 37.0 37.0 40.0 27.0 40.0 48-49 35.472151791715454 37.0 37.0 40.0 27.0 40.0 50-51 34.310886832571725 37.0 35.0 38.5 24.5 38.5 52-53 34.78027760281703 37.0 35.0 38.5 27.0 40.0 54-55 35.572094301258446 37.0 37.0 40.0 27.0 40.0 56-57 35.63035876581516 37.0 37.0 40.0 27.0 40.0 58-59 35.420012808535645 37.0 37.0 40.0 27.0 40.0 60-61 35.16012888853192 37.0 37.0 40.0 27.0 40.0 62-63 34.92793761439967 37.0 35.0 40.0 27.0 40.0 64-65 34.66224652415233 37.0 33.0 40.0 27.0 40.0 66-67 34.406345509192136 37.0 33.0 37.0 27.0 40.0 68-69 34.1998059697076 37.0 33.0 37.0 27.0 40.0 70-71 33.89952443555785 37.0 33.0 37.0 27.0 40.0 72-73 33.67042411893761 37.0 33.0 37.0 27.0 38.5 74-75 33.41449837462642 37.0 33.0 37.0 27.0 37.0 76-77 33.17689454305655 37.0 33.0 37.0 27.0 37.0 78-79 32.92497495360605 37.0 33.0 37.0 24.5 37.0 80-81 32.71069618407091 37.0 33.0 37.0 22.0 37.0 82-83 32.527451481858805 37.0 33.0 37.0 22.0 37.0 84-85 32.36303321342064 37.0 33.0 37.0 22.0 37.0 86-87 32.16526540723112 33.0 33.0 37.0 22.0 37.0 88-89 32.05765637615668 33.0 33.0 37.0 22.0 37.0 90-91 31.93909710391823 33.0 33.0 37.0 22.0 37.0 92-93 31.764223181335893 33.0 33.0 37.0 22.0 37.0 94-95 31.625617282645912 33.0 33.0 37.0 22.0 37.0 96-97 31.459287456512275 33.0 33.0 37.0 15.0 37.0 98-99 31.33034312502906 33.0 33.0 37.0 15.0 37.0 100-101 30.056277239274436 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08845166819196493 2306 2 0.07632735834589965 2306 3 0.13619705310670582 2306 4 0.2008944578799614 2306 5 0.13665996474516362 2306 6 0.19332791587584808 2306 7 0.16748618802582627 2306 8 0.09274679260674645 2306 9 0.24777047113318673 2306 10-11 0.3315460967926498 2306 12-13 0.24366010281834605 2306 14-15 0.5642170304917045 2306 16-17 0.5922625667803985 2306 18-19 0.48015777260699366 2306 20-21 0.5080596434465932 2306 22-23 0.47334373883644076 2306 24-25 0.39086981195728043 2306 26-27 0.4136328985296842 2306 28-29 0.3705131654152538 2306 30-31 0.6029227677105382 2306 32-33 0.6206695555227455 2306 34-35 0.6436074635534936 2306 36-37 0.7157201154688195 2306 38-39 0.6725849269750483 2306 40-41 0.6105312479144303 2306 42-43 0.5740843724885778 2306 44-45 0.5786988971678184 2306 46-47 0.41813892580845646 2306 48-49 0.6502591238469009 2306 50-51 0.6128228958687032 2306 52-53 0.6075677255085665 2306 54-55 0.6730264503101395 2306 56-57 0.7527088478326078 2306 58-59 0.7894252247083742 2306 60-61 0.7116738811984433 2306 62-63 0.758912716819097 2306 64-65 0.8142897229878727 2306 66-67 0.7870571202374634 2306 68-69 0.6487079518350711 2306 70-71 0.7374440815162657 2306 72-73 0.7193646243679765 2306 74-75 0.7840349641598294 2306 76-77 0.7482004443087433 2306 78-79 0.6513099081736158 2306 80-81 0.6314047759988668 2306 82-83 0.5645975610733984 2306 84-85 0.5331193024573508 2306 86-87 0.6143745779724199 2306 88-89 0.8142939201487849 2306 90-91 0.8043509777379505 2306 92-93 0.5411118059943192 2306 94-95 0.7694431627069598 2306 96-97 0.6165609126555687 2306 98-99 0.6339072468626306 2306 100-101 0.3099166904656734 2307 1 -0.049232155006201594 2307 2 -0.13101241521887985 2307 3 -0.015961073135525083 2307 4 0.07617218610364063 2307 5 -0.045380186692177915 2307 6 0.0665072530762032 2307 7 0.05676340088682963 2307 8 0.045502811628317374 2307 9 0.08148492056908196 2307 10-11 0.03189648935124012 2307 12-13 -0.011890857720374015 2307 14-15 0.11224351580836611 2307 16-17 0.23176232261381813 2307 18-19 0.14584281753905515 2307 20-21 0.11601100474285175 2307 22-23 0.12491937912049877 2307 24-25 -0.010576537166691935 2307 26-27 -0.1049899839333861 2307 28-29 -0.1503202805410453 2307 30-31 0.11551536630967973 2307 32-33 0.16791443748486756 2307 34-35 0.22477933700492514 2307 36-37 0.17567544469726926 2307 38-39 0.12895434110139092 2307 40-41 0.07714394565783067 2307 42-43 0.09721532686456413 2307 44-45 0.05167073782344289 2307 46-47 0.14608780038972924 2307 48-49 0.08294077567436631 2307 50-51 -0.0983753403656209 2307 52-53 -0.008633042489940124 2307 54-55 -0.021176080012466514 2307 56-57 0.10808699969881985 2307 58-59 0.2534453418722862 2307 60-61 0.26842098768931066 2307 62-63 0.14201864186807 2307 64-65 0.209741377103839 2307 66-67 0.004985254174094678 2307 68-69 -0.05736954357441704 2307 70-71 -0.023829026463026537 2307 72-73 -0.2823895908842431 2307 74-75 -0.05144451233260838 2307 76-77 -0.08716235479531775 2307 78-79 -0.22846237720756335 2307 80-81 -0.06885912212510448 2307 82-83 -0.0676988301265169 2307 84-85 -0.05207861663071611 2307 86-87 -0.16325814143062445 2307 88-89 -0.13130062998104108 2307 90-91 -0.1098870873815514 2307 92-93 -0.15469222284436768 2307 94-95 -0.10635623507553404 2307 96-97 0.062045542774733065 2307 98-99 -3.285117386084835E-4 2307 100-101 -0.24110015973137067 2201 1 0.10576787397596021 2201 2 0.05755185588764533 2201 3 -0.0011311641763995794 2201 4 0.09784952611024522 2201 5 0.07621790027433661 2201 6 0.15566639623702372 2201 7 0.21020208174526545 2201 8 0.12337471995615346 2201 9 0.16817056791729357 2201 10-11 0.09469490681979664 2201 12-13 -0.013001269746595767 2201 14-15 0.06301322934341158 2201 16-17 0.10900191920320168 2201 18-19 0.1418708122000112 2201 20-21 0.1673405219158397 2201 22-23 -0.005438829866179162 2201 24-25 0.09675455347423423 2201 26-27 0.10844453930213405 2201 28-29 0.013763548519001745 2201 30-31 0.24071606778340282 2201 32-33 0.3127504221590982 2201 34-35 0.31836919409124675 2201 36-37 0.3092543623347055 2201 38-39 0.2974562373252212 2201 40-41 0.17795220546867796 2201 42-43 0.18618872071265713 2201 44-45 0.1958252464730421 2201 46-47 0.2294101995436577 2201 48-49 0.12118998432959671 2201 50-51 0.21065842596121342 2201 52-53 0.164850492216722 2201 54-55 0.3562164633514371 2201 56-57 0.30487416892671604 2201 58-59 0.41922699869292757 2201 60-61 0.40141551954571497 2201 62-63 0.29634720684528304 2201 64-65 0.33676202663098564 2201 66-67 0.3339915506805795 2201 68-69 0.20738066443846748 2201 70-71 0.17417091451876132 2201 72-73 0.22904387213109345 2201 74-75 0.12098011955441024 2201 76-77 0.2720089714126175 2201 78-79 0.2705876665554996 2201 80-81 0.23469306341974772 2201 82-83 0.13946737835851764 2201 84-85 0.14374384180491262 2201 86-87 0.1707762967554345 2201 88-89 0.2640383269456663 2201 90-91 0.1787865782138276 2201 92-93 0.06224914202577736 2201 94-95 0.1664681398714407 2201 96-97 0.3413048799052554 2201 98-99 0.21111796690232865 2201 100-101 -0.055556752093043116 1101 1 0.04108685113812882 1101 2 -0.03603252832880699 1101 3 0.013330536662952142 1101 4 0.16441672290647347 1101 5 0.07357830646258634 1101 6 0.13668131337753664 1101 7 0.1588616958168032 1101 8 0.08399910890593532 1101 9 0.11025123775955592 1101 10-11 0.12650256097384016 1101 12-13 0.06677928419592405 1101 14-15 0.2351373389218665 1101 16-17 0.23276926181646473 1101 18-19 0.11505748711818597 1101 20-21 0.23692509603799294 1101 22-23 0.16033632178401547 1101 24-25 -0.05576787615449774 1101 26-27 0.13244124792142742 1101 28-29 -0.033243957527680834 1101 30-31 0.1609395696158913 1101 32-33 0.083839378665445 1101 34-35 0.15493672966397298 1101 36-37 0.1537700240678035 1101 38-39 0.07231670673584745 1101 40-41 0.12735354921851894 1101 42-43 -0.06183921832857209 1101 44-45 0.06601384478287287 1101 46-47 -0.05758324012096239 1101 48-49 0.0582717921746152 1101 50-51 0.27468074322111846 1101 52-53 0.24496159182321975 1101 54-55 0.3762527076655928 1101 56-57 0.38177369115641824 1101 58-59 0.3523143452951629 1101 60-61 0.3555040270539038 1101 62-63 0.2718293311361819 1101 64-65 0.36455661069216916 1101 66-67 0.29507925228242016 1101 68-69 0.2959570015201294 1101 70-71 0.30769967258795816 1101 72-73 0.14292104036034203 1101 74-75 0.20078810962205296 1101 76-77 0.24060200397286025 1101 78-79 0.17838701734520868 1101 80-81 0.10730928165289555 1101 82-83 0.1258491594354112 1101 84-85 0.2121751443719333 1101 86-87 0.18941628087588214 1101 88-89 0.169082017185616 1101 90-91 0.22992536826639665 1101 92-93 0.09593473930067731 1101 94-95 0.1266336298066797 1101 96-97 0.3067623212265431 1101 98-99 0.12776534875640522 1101 100-101 -0.09796870718178141 2304 1 0.005954370227144068 2304 2 0.016323757361906388 2304 3 0.006714697149956095 2304 4 0.1884679498641404 2304 5 0.19678861430871564 2304 6 0.008972323376482905 2304 7 0.15950487163268434 2304 8 0.03659660770608042 2304 9 0.07597395889892056 2304 10-11 0.051706314636618345 2304 12-13 0.039650350066885665 2304 14-15 0.10167569570967316 2304 16-17 0.12928537802813622 2304 18-19 0.2828815745404185 2304 20-21 0.24102190420038738 2304 22-23 0.20441781792629854 2304 24-25 0.038465143338612506 2304 26-27 0.05876811962958328 2304 28-29 -0.022601172052993945 2304 30-31 0.0761308501035387 2304 32-33 0.09688010100955324 2304 34-35 0.0850091024681916 2304 36-37 0.20098423000293764 2304 38-39 0.10525573632992291 2304 40-41 0.17049645223914212 2304 42-43 0.2384901764030687 2304 44-45 0.3093104002880622 2304 46-47 0.3167821227765799 2304 48-49 0.3372439794780533 2304 50-51 0.15409969375335208 2304 52-53 0.35008867892874207 2304 54-55 0.481248498190773 2304 56-57 0.39141068272594026 2304 58-59 0.4573112447441616 2304 60-61 0.5225622916857091 2304 62-63 0.6274900323841166 2304 64-65 0.4738264154062435 2304 66-67 0.5062006049210908 2304 68-69 0.4935301882744412 2304 70-71 0.389851188841682 2304 72-73 0.42761993205752447 2304 74-75 0.47141520160684536 2304 76-77 0.4322550900191331 2304 78-79 0.4893540965019625 2304 80-81 0.322135770560557 2304 82-83 0.42319044804037276 2304 84-85 0.47905096885679654 2304 86-87 0.3228772587337758 2304 88-89 0.08793718197613032 2304 90-91 0.15850801281018434 2304 92-93 0.2791710935494933 2304 94-95 0.3209840621538973 2304 96-97 0.3454003321188388 2304 98-99 0.41472821932548243 2304 100-101 0.27266401813601604 2202 1 0.04879186464815888 2202 2 0.07157206048108833 2202 3 0.0373239678286339 2202 4 0.08903657158371914 2202 5 -0.02244706270884933 2202 6 0.09238508047555882 2202 7 0.07653470709092858 2202 8 0.110606446592449 2202 9 0.16054474348428016 2202 10-11 0.1178534077267912 2202 12-13 -0.013719315326511605 2202 14-15 0.05819066643397974 2202 16-17 0.08873619891519269 2202 18-19 0.011104902853013243 2202 20-21 0.10427053357113891 2202 22-23 0.010099191031400778 2202 24-25 -0.14667848601972366 2202 26-27 -0.017146854572523296 2202 28-29 0.06981786745905794 2202 30-31 0.06101438905541556 2202 32-33 0.04692614057211131 2202 34-35 0.05835997941422022 2202 36-37 -0.020958755463965417 2202 38-39 -0.027763407350775537 2202 40-41 -0.08426069320861274 2202 42-43 0.08931102205708186 2202 44-45 0.1843448678562538 2202 46-47 0.10001713901049669 2202 48-49 0.055156847121757835 2202 50-51 0.09093999691890531 2202 52-53 0.02180610366109903 2202 54-55 -0.008281020538468908 2202 56-57 0.22986874656359646 2202 58-59 0.16778222284472832 2202 60-61 0.27972243732309465 2202 62-63 0.14525211217995349 2202 64-65 0.2447986368234254 2202 66-67 0.2756765405551249 2202 68-69 0.24826828857940342 2202 70-71 0.2062613947196681 2202 72-73 0.1458843067930644 2202 74-75 0.041817849047305344 2202 76-77 -0.08777439683019139 2202 78-79 -0.03997356027475263 2202 80-81 -0.11897851106080282 2202 82-83 0.041331401970182924 2202 84-85 0.0751266757835154 2202 86-87 0.01647900161275828 2202 88-89 0.1006098613875821 2202 90-91 -0.09772844340213638 2202 92-93 -0.16812168080613787 2202 94-95 0.0752323710694256 2202 96-97 0.024601462903486748 2202 98-99 -0.07196067495493352 2202 100-101 -0.09289300674770473 1102 1 -0.0693027995117852 1102 2 -0.10486217102607398 1102 3 -0.21586000894881252 1102 4 -0.04394835142636566 1102 5 -0.08936410589195276 1102 6 0.02448847084031769 1102 7 -0.05025896241746608 1102 8 -0.16237353035938185 1102 9 -0.07649702755951893 1102 10-11 -0.022986179875346124 1102 12-13 -0.11614776434372232 1102 14-15 -0.24581299797018374 1102 16-17 -0.2063960039750512 1102 18-19 -0.13428654370698467 1102 20-21 -0.23187141752924134 1102 22-23 -0.057225651182776005 1102 24-25 -0.1810918823529235 1102 26-27 -0.17539130096316313 1102 28-29 -0.10648403191219558 1102 30-31 0.013250300601981735 1102 32-33 0.13290190488664422 1102 34-35 0.03731405784820652 1102 36-37 -0.1222942104839646 1102 38-39 0.012774690608182482 1102 40-41 -0.24597991913892514 1102 42-43 -0.10229240746718204 1102 44-45 -0.05083991294501544 1102 46-47 -0.005577636069240555 1102 48-49 -0.12260581770022583 1102 50-51 0.16131773765054902 1102 52-53 0.09247975911065254 1102 54-55 0.17704164803357259 1102 56-57 0.2596959470290372 1102 58-59 0.38642143567300735 1102 60-61 0.4015062938056104 1102 62-63 0.2844973483195119 1102 64-65 0.25057080641072105 1102 66-67 0.19780216503356485 1102 68-69 0.32427352090718387 1102 70-71 0.16225700499651197 1102 72-73 0.13425094242791147 1102 74-75 0.09308092649092714 1102 76-77 0.08896606975825705 1102 78-79 0.08281474727439075 1102 80-81 0.06882017665194695 1102 82-83 -0.07674291081900719 1102 84-85 0.09691026220044563 1102 86-87 0.043320434148640174 1102 88-89 -0.06286353695954006 1102 90-91 -0.058633074371019944 1102 92-93 -0.018470914186394793 1102 94-95 0.039193083471570134 1102 96-97 0.12312215937948068 1102 98-99 -0.029761287849485285 1102 100-101 -0.044984626111098436 2305 1 0.02621037061835807 2305 2 0.007691461457728366 2305 3 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-0.6586651370230285 1213 78-79 -0.44465543249601325 1213 80-81 -0.49364239566546786 1213 82-83 -0.6154743904905686 1213 84-85 -0.5246144596915201 1213 86-87 -0.5127679665760283 1213 88-89 -0.5747766340851612 1213 90-91 -0.4973680528090263 1213 92-93 -0.3677335187627051 1213 94-95 -0.44586839958122937 1213 96-97 -0.39343956905977606 1213 98-99 -0.3095521696858725 1213 100-101 -0.38373661206631837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2390.0 3 221.0 4 215.0 5 211.0 6 488.0 7 2704.0 8 11621.0 9 16345.0 10 15472.0 11 12540.0 12 9278.0 13 7060.0 14 5558.0 15 4998.0 16 4999.0 17 5530.0 18 5968.0 19 6778.0 20 7639.0 21 8799.0 22 9626.0 23 10783.0 24 12121.0 25 14272.0 26 17249.0 27 20331.0 28 24294.0 29 29939.0 30 37919.0 31 49950.0 32 68348.0 33 97498.0 34 148771.0 35 248946.0 36 480649.0 37 771763.0 38 194223.0 39 114.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.07155530775866 24.880950161755262 26.313022272911375 14.734472257574705 2 29.42773414824099 22.682458044882633 35.082342452739844 12.80746535413653 3 38.28710990042174 19.82643176845142 26.04447023984783 15.84198809127901 4 28.030417600218925 18.779661332869047 36.60007786132973 16.589843205582294 5 31.635050609980325 17.69951245634222 34.275912089625336 16.38952484405212 6 24.284384947337376 18.51234444440678 41.16892549264215 16.034345115613696 7 32.36615464916859 18.638109150561615 31.774628696931284 17.221107503338505 8 27.551964457277055 27.770505775523034 27.79850651430198 16.87902325289793 9 30.609964478534206 25.730697645654526 25.413096941494207 18.246240934317058 10-11 24.782197416883918 28.585401842880593 28.81497310304067 17.81742763719482 12-13 25.64167086592391 25.468214535127924 28.861955746077218 20.02815885287095 14-15 24.271291900080318 26.100772001884252 30.019219559640366 19.60871653839506 16-17 23.159270195969032 27.56673599368622 31.04505451374393 18.228939296600817 18-19 22.189841807318505 27.909667961200828 31.182191298925986 18.718298932554678 20-21 22.138662146502124 28.43364354402586 30.63256466817113 18.795129641300885 22-23 22.80348050683293 27.808932030613214 30.53477860462162 18.85280885793223 24-25 22.605938152773888 27.951180531688514 30.54770450873594 18.895176806801654 26-27 21.99020419660159 28.608310397838917 30.367308690827272 19.034176714732222 28-29 21.457879747689038 28.596672653944072 30.146440861989536 19.79900673637735 30-31 21.57999978373652 28.696213205260122 29.908264000039015 19.81552301096435 32-33 20.826673449053924 28.49033265870076 31.427710195493546 19.255283696751775 34-35 20.552852999624953 29.26501085265003 31.17016429164185 19.011971856083168 36-37 20.917515506972677 30.057284256948808 30.66001850780896 18.365181728269555 38-39 21.87510275307167 29.191927570195062 29.802622884188303 19.13034679254497 40-41 21.486236986760243 28.579390070383347 29.456142945001197 20.478229997855212 42-43 21.84493880995682 28.743698028367998 30.295778761527792 19.11558440014739 44-45 21.045605728832626 29.274024111149842 30.839596123495017 18.840774036522518 46-47 21.276423726892965 29.20531366829594 30.605263504116476 18.912999100694616 48-49 21.24830007106334 29.79847845087431 29.721961755657112 19.231259722405238 50-51 20.740465477160143 29.50613987914215 29.79862826301119 19.954766380686515 52-53 21.29109579446988 29.01723673945137 29.810288900453248 19.881378565625507 54-55 21.941925869601377 29.11866193238716 29.339393188476148 19.60001900953532 56-57 22.23780868192447 29.012896846447322 28.75203170952177 19.997262762106445 58-59 21.745619570480475 28.633998557998545 29.825347228263567 19.795034643257413 60-61 20.682724833458856 29.135394359287716 29.871316492117227 20.310564315136205 62-63 21.309890165806063 29.177711746990187 29.45621420264997 20.05618388455378 64-65 21.055130650724692 29.129868188194724 29.55101703236287 20.26398412871771 66-67 21.102773977084418 28.827965147070394 29.362814789143 20.706446086702186 68-69 21.21194576814674 28.767419321317156 29.952346717395038 20.068288193141065 70-71 20.651157386745627 30.22278467808601 29.581032337372314 19.545025597796048 72-73 20.30719689983613 31.20148309261697 28.909621658748787 19.581698348798113 74-75 21.06472883008947 30.56150616969851 28.582453462239748 19.791311537972266 76-77 21.449082566181247 29.680638422069382 29.177124297615077 19.69315471413429 78-79 21.391784877057308 29.580401266801175 29.72807457459633 19.299739281545182 80-81 20.394114306339564 30.403224814513365 29.47153962922607 19.731121249921006 82-83 19.59545550284153 30.19174523394344 29.722918708666406 20.489880554548623 84-85 19.71068937393756 29.493440131171916 30.270682109240337 20.525188385650186 86-87 19.66722815382471 29.705146041473103 31.002323534148275 19.62530227055392 88-89 19.962392232508762 30.273489048641867 30.409505858630776 19.354612860218595 90-91 20.389196682656955 29.602605617238037 29.851272722105175 20.156924977999832 92-93 19.715950959924594 30.609035494997066 29.689327326493114 19.985686218585226 94-95 19.26724851631308 31.133712135586926 29.813154986318047 19.785884361781946 96-97 19.93211630093 31.549147403725225 29.181424030965182 19.33731226437959 98-99 20.426935968089037 30.50919501855718 28.76873587637429 20.295133136979494 100-101 20.322509064773353 30.28487105662206 28.343316676043283 21.049303202561305 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1827.0 1 1143.5 2 363.0 3 255.5 4 230.0 5 232.0 6 323.0 7 456.5 8 578.0 9 708.0 10 833.5 11 900.5 12 896.0 13 834.5 14 755.5 15 696.5 16 627.0 17 630.0 18 659.5 19 736.5 20 930.5 21 1132.5 22 1466.0 23 1947.0 24 2559.5 25 3376.5 26 4564.0 27 6366.5 28 8312.0 29 10057.0 30 12360.5 31 15134.0 32 20428.0 33 30494.5 34 46347.0 35 69101.0 36 99941.0 37 138294.0 38 177500.0 39 196782.0 40 193202.0 41 172005.5 42 146469.0 43 137786.5 44 131051.0 45 122125.0 46 120581.0 47 107564.5 48 80273.5 49 60660.0 50 48745.0 51 40289.0 52 33792.5 53 26914.5 54 20654.5 55 15209.0 56 11085.0 57 8003.0 58 6164.5 59 4894.0 60 3821.5 61 3021.5 62 2332.5 63 1833.5 64 1483.0 65 1137.5 66 886.0 67 767.5 68 640.0 69 480.0 70 381.0 71 319.5 72 256.5 73 191.5 74 133.0 75 110.0 76 94.0 77 74.5 78 64.0 79 48.5 80 34.0 81 34.0 82 26.5 83 20.5 84 16.0 85 10.0 86 8.5 87 6.5 88 7.0 89 8.0 90 4.5 91 2.0 92 3.0 93 5.5 94 4.0 95 2.5 96 2.0 97 0.5 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22209916258385787 2 0.23621814246642514 3 0.21791419549291724 4 0.21148879147450342 5 0.22007008763067454 6 0.24019174758307585 7 0.22294461048101757 8 0.20958653370589403 9 0.2022311370006045 10-11 0.18893646881776793 12-13 0.2144689953119914 14-15 0.21110833992078154 16-17 0.21383490938912164 18-19 0.20233681798774947 20-21 0.1958902777719066 22-23 0.16422825402327518 24-25 0.4951365609715887 26-27 0.34976179505497523 28-29 0.48408232971622545 30-31 0.3117800482750749 32-33 0.3603510299669007 34-35 0.3627816926712349 36-37 0.3706020857199623 38-39 0.3645782694526993 40-41 0.36910141570250377 42-43 0.4226394037901429 44-45 0.3762031780386454 46-47 0.3951200747375941 48-49 0.3915057849772363 50-51 0.4066181661389663 52-53 0.4105494988607589 54-55 0.37613976944635846 56-57 0.3897514805906299 58-59 0.3873419540837247 60-61 0.3449216058437359 62-63 0.30784871555328225 64-65 0.3336348764166536 66-67 0.34741567714035704 68-69 0.33627690109527775 70-71 0.308651891055584 72-73 0.2969635738773509 74-75 0.30901120641187685 76-77 0.3163454669197374 78-79 0.3256031213936363 80-81 0.3336560126140826 82-83 0.3526151817078893 84-85 0.3807897328807369 86-87 0.383431757559361 88-89 0.3769006725538022 90-91 0.34838794222209074 92-93 0.32729401718795575 94-95 0.30484737551836527 96-97 0.3215449714872697 98-99 0.35090314971614084 100-101 0.3670934769467495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2365610.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.16144063648726 #Duplication Level Percentage of deduplicated Percentage of total 1 88.91241686453978 39.26500419209981 2 4.711061161967006 4.160944956781332 3 1.7274214644227417 2.288562613658964 4 0.9603498232896438 1.6964172684586651 5 0.58453148341368 1.290687620246553 6 0.4325594336929033 1.1461468651669016 7 0.36812335611946867 1.1379800416722061 8 0.24506879564366413 0.8658072856538478 9 0.23159904544997312 0.9204972747395491 >10 1.4229419074428489 12.317581058963842 >50 0.19728685760572262 6.055537853455799 >100 0.17591647162498183 16.901088900556104 >500 0.02444134812061122 7.256216363121556 >1k 0.006184382154138504 4.455458118083285 >5k 9.760451284722147E-5 0.24206958734151934 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5616 0.23740176952244876 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4635 0.19593255016676459 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4138 0.1749231699223456 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3540 0.14964427779727005 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3502 0.14803792679266659 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3436 0.14524794873203953 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2874 0.12149086282185145 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2870 0.1213217732424195 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2754 0.11641817543889313 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2667 0.11274047708624838 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2431 0.1027641918997637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010568098714496472 0.0 0.0 2 0.001141354661165619 0.0 0.0 3 0.0012681718457395767 0.0 0.0 4 0.0013527166354555485 0.0 0.0 5 0.0017331681891774215 0.0 0.0 6 0.0018599853737513792 0.0 0.0 7 0.0019445301634673509 0.0 0.0 8 0.0021136197428992943 0.0 0.0 9 0.0021981645326152664 0.0 0.0 10-11 0.0032127020092069278 0.0 0.0 12-13 0.004184967090940603 0.0 0.0 14-15 0.005854726687831046 0.0 0.0 16-17 0.007587894877008467 0.0 0.0 18-19 0.009574697435333804 0.0 0.0 20-21 0.011688317178233099 0.0 0.0 22-23 0.014182388474854266 0.0 0.0 24-25 0.01650737019204349 0.0 0.0 26-27 0.018959169093806672 0.0 0.0 28-29 0.021875964339007698 0.0 0.0 30-31 0.03514949632441527 0.0 0.0 32-33 0.03922878242821091 0.0 0.0 34-35 0.04296988937314265 0.0 0.0 36-37 0.04715485646408326 0.0 0.0 38-39 0.05161459412160077 0.0 0.0 40-41 0.05618001276626325 0.0 0.0 42-43 0.06121042775436357 0.0 0.0 44-45 0.06704401824476562 0.0 0.0 46-47 0.07268738295830673 0.0 0.0 48-49 0.07947210233301347 0.0 0.0 50-51 0.08577068916685338 0.0 0.0 52-53 0.09291472389785299 0.0 0.0 54-55 0.10143261146173714 0.0 0.0 56-57 0.11037322297420116 0.0 0.0 58-59 0.11984223942239 0.0 0.0 60-61 0.13036806574202847 0.0 0.0 62-63 0.14224660869712252 0.0 0.0 64-65 0.1540406068625006 0.0 0.0 66-67 0.16638414616103248 0.0 0.0 68-69 0.18111607576904054 0.0 0.0 70-71 0.19663004468192136 0.0 2.1136197428992946E-5 72-73 0.212778099517672 0.0 8.454478971597178E-5 74-75 0.230765003529745 0.0 8.454478971597178E-5 76-77 0.2510346168641492 0.0 8.454478971597178E-5 78-79 0.27434784262832845 0.0 8.454478971597178E-5 80-81 0.2997746881354069 0.0 8.454478971597178E-5 82-83 0.3270615190162368 0.0 8.454478971597178E-5 84-85 0.3581740016317144 0.0 8.454478971597178E-5 86-87 0.3939998562738575 0.0 8.454478971597178E-5 88-89 0.4334188644789293 0.0 8.454478971597178E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGC 20 0.0020858478 71.150764 2 CGGGAGA 695 0.0 67.56619 4 GAGCGGC 540 0.0 61.470078 9 GAGAGCG 785 0.0 60.412476 7 TCGGAGA 220 0.0 58.21302 4 AGAGCGG 610 0.0 56.749454 8 ATTCGGA 130 0.0 54.731354 2 CGGAAAG 345 0.0 53.619724 5 TCGGGAG 890 0.0 52.762367 3 TTGTGTT 30040 0.0 51.402637 1 TCGGGAA 285 0.0 49.9293 3 AAGCGGC 240 0.0 49.395596 9 ACCGGAA 145 0.0 49.06845 3 GGATCGG 395 0.0 48.03936 1 TCGACGG 40 5.4265006E-4 47.43384 2 GATTCGC 60 1.5104488E-6 47.419773 9 TTCGGAG 165 0.0 45.995476 3 CGGAGAG 485 0.0 44.987846 5 GGACCGG 265 0.0 44.75365 1 GTTCGGA 170 0.0 41.85339 2 >>END_MODULE