FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365610
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56160.23740176952244876No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG46350.19593255016676459No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC41380.1749231699223456No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA35400.14964427779727005No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG35020.14803792679266659No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA34360.14524794873203953No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC28740.12149086282185145No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28700.1213217732424195No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA27540.11641817543889313No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT26670.11274047708624838No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG24310.1027641918997637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC200.002085847871.1507642
CGGGAGA6950.067.566194
GAGCGGC5400.061.4700789
GAGAGCG7850.060.4124767
TCGGAGA2200.058.213024
AGAGCGG6100.056.7494548
ATTCGGA1300.054.7313542
CGGAAAG3450.053.6197245
TCGGGAG8900.052.7623673
TTGTGTT300400.051.4026371
TCGGGAA2850.049.92933
AAGCGGC2400.049.3955969
ACCGGAA1450.049.068453
GGATCGG3950.048.039361
TCGACGG405.4265006E-447.433842
GATTCGC601.5104488E-647.4197739
TTCGGAG1650.045.9954763
CGGAGAG4850.044.9878465
GGACCGG2650.044.753651
GTTCGGA1700.041.853392
Produced by FastQC (version 0.11.2)