##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2578654 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04071736650206 33.0 33.0 33.0 33.0 33.0 2 31.99352181409371 33.0 33.0 33.0 33.0 33.0 3 31.981879693824762 33.0 33.0 33.0 33.0 33.0 4 35.71342646202243 37.0 37.0 37.0 33.0 37.0 5 35.69424630058938 37.0 37.0 37.0 33.0 37.0 6 35.704846404364446 37.0 37.0 37.0 33.0 37.0 7 35.664517612677 37.0 37.0 37.0 33.0 37.0 8 35.59628356499166 37.0 37.0 37.0 33.0 37.0 9 35.510798656973755 37.0 37.0 37.0 33.0 37.0 10-11 35.45322133174904 37.0 37.0 37.0 33.0 37.0 12-13 35.48088906072703 37.0 37.0 37.0 33.0 37.0 14-15 37.22400426734257 40.0 37.0 40.0 33.0 40.0 16-17 37.18666870390521 40.0 37.0 40.0 33.0 40.0 18-19 37.18292663536869 40.0 37.0 40.0 33.0 40.0 20-21 37.13602406526816 40.0 37.0 40.0 33.0 40.0 22-23 37.18605074585423 40.0 37.0 40.0 33.0 40.0 24-25 36.97439982254308 40.0 37.0 40.0 33.0 40.0 26-27 36.877035655035534 40.0 37.0 40.0 33.0 40.0 28-29 36.77125469334001 40.0 37.0 40.0 33.0 40.0 30-31 36.81096203678353 40.0 37.0 40.0 33.0 40.0 32-33 36.72343594759126 40.0 37.0 40.0 33.0 40.0 34-35 36.64005620761839 40.0 37.0 40.0 33.0 40.0 36-37 36.571760305958065 40.0 37.0 40.0 33.0 40.0 38-39 36.5112000291625 40.0 37.0 40.0 33.0 40.0 40-41 36.39312738351093 40.0 37.0 40.0 33.0 40.0 42-43 36.28503106659521 40.0 37.0 40.0 33.0 40.0 44-45 36.202017990781236 37.0 37.0 40.0 33.0 40.0 46-47 36.096044486774886 37.0 37.0 40.0 33.0 40.0 48-49 35.957606565285616 37.0 37.0 40.0 27.0 40.0 50-51 34.7772463851296 37.0 35.0 38.5 27.0 38.5 52-53 35.2267491101947 37.0 37.0 38.5 27.0 40.0 54-55 36.025911386327905 37.0 37.0 40.0 33.0 40.0 56-57 36.06677126904191 37.0 37.0 40.0 30.0 40.0 58-59 35.88204660260741 37.0 37.0 40.0 27.0 40.0 60-61 35.623342061401026 37.0 37.0 40.0 27.0 40.0 62-63 35.365571534606815 37.0 37.0 40.0 27.0 40.0 64-65 35.09653078699198 37.0 37.0 40.0 27.0 40.0 66-67 34.84157684590488 37.0 33.0 37.0 27.0 40.0 68-69 34.621175427180226 37.0 33.0 37.0 27.0 40.0 70-71 34.32626343045635 37.0 33.0 37.0 27.0 40.0 72-73 34.09615714244718 37.0 33.0 37.0 27.0 38.5 74-75 33.83649027748585 37.0 33.0 37.0 27.0 37.0 76-77 33.612809822488785 37.0 33.0 37.0 27.0 37.0 78-79 33.3667310542632 37.0 33.0 37.0 27.0 37.0 80-81 33.13948051968197 37.0 33.0 37.0 27.0 37.0 82-83 32.946380165776404 37.0 33.0 37.0 27.0 37.0 84-85 32.789221237126036 37.0 33.0 37.0 27.0 37.0 86-87 32.58822548507865 37.0 33.0 37.0 24.5 37.0 88-89 32.469684184074325 35.0 33.0 37.0 22.0 37.0 90-91 32.36917263812826 33.0 33.0 37.0 22.0 37.0 92-93 32.214260230337224 33.0 33.0 37.0 22.0 37.0 94-95 32.08579243279634 33.0 33.0 37.0 22.0 37.0 96-97 31.9090769835736 33.0 33.0 37.0 22.0 37.0 98-99 31.780034273694724 33.0 33.0 37.0 22.0 37.0 100-101 30.474580730877427 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09766147551278692 2306 2 0.02922744221000073 2306 3 0.04113885935673878 2306 4 0.13723895234574002 2306 5 0.19172036928018343 2306 6 0.1497309330412122 2306 7 0.16802097468895028 2306 8 0.15857235384239488 2306 9 0.20689798366442602 2306 10-11 0.30114373126863825 2306 12-13 0.18641676843449773 2306 14-15 0.4945603442386144 2306 16-17 0.5073261083978196 2306 18-19 0.4714686469802487 2306 20-21 0.44285265842852084 2306 22-23 0.40096229582240284 2306 24-25 0.4511112645235187 2306 26-27 0.36077631446946157 2306 28-29 0.36286882245394736 2306 30-31 0.48895728149113893 2306 32-33 0.4419898501810451 2306 34-35 0.4860404674521419 2306 36-37 0.5725934826392276 2306 38-39 0.35232402050942113 2306 40-41 0.5762467694718225 2306 42-43 0.6577921799874886 2306 44-45 0.5670179129324069 2306 46-47 0.5423454051905452 2306 48-49 0.6057069414894585 2306 50-51 0.5035679526200312 2306 52-53 0.4785532366629752 2306 54-55 0.7297044867107161 2306 56-57 0.5832784523374599 2306 58-59 0.5832431417105468 2306 60-61 0.4903535837871402 2306 62-63 0.5926312818990525 2306 64-65 0.44938639042999284 2306 66-67 0.5220354918037984 2306 68-69 0.5201596269346211 2306 70-71 0.5088759287192346 2306 72-73 0.3749937048452736 2306 74-75 0.49345832604355877 2306 76-77 0.5054080513493133 2306 78-79 0.44012612017554176 2306 80-81 0.30636706338385267 2306 82-83 0.349759599410568 2306 84-85 0.33318084258878855 2306 86-87 0.5206202597846996 2306 88-89 0.37803506503195194 2306 90-91 0.5190338515999784 2306 92-93 0.42683691606033847 2306 94-95 0.45553169982586184 2306 96-97 0.33980944813001557 2306 98-99 0.3112424470216375 2306 100-101 0.009029710487748588 2307 1 0.16917679591861656 2307 2 0.046844154876914956 2307 3 0.04829088549238847 2307 4 0.1697242209012373 2307 5 0.23788688327147867 2307 6 0.22945440779443516 2307 7 0.2623125955283001 2307 8 0.22181751096956503 2307 9 0.2945292029625932 2307 10-11 0.1376650895162186 2307 12-13 0.09884570521107605 2307 14-15 0.2599399179531545 2307 16-17 0.2825536144727181 2307 18-19 0.17429059685726855 2307 20-21 0.1824755502483768 2307 22-23 0.11287473031774908 2307 24-25 0.21175444289875145 2307 26-27 0.2599905550799235 2307 28-29 0.11989880710734724 2307 30-31 0.1674974232569042 2307 32-33 0.20082266422046047 2307 34-35 0.32982860541534365 2307 36-37 0.45675511694760473 2307 38-39 0.28457024587013535 2307 40-41 0.18518645627484887 2307 42-43 0.3178219146287944 2307 44-45 0.10042911564874402 2307 46-47 0.2981833854880023 2307 48-49 0.24654960580818397 2307 50-51 0.0828216664856285 2307 52-53 0.03208571995515541 2307 54-55 0.17079871953424686 2307 56-57 0.19788071568815724 2307 58-59 0.14128461308613538 2307 60-61 0.2789876184390181 2307 62-63 0.18863531272663892 2307 64-65 0.21539012974147909 2307 66-67 0.13298861601633405 2307 68-69 0.05299965575024146 2307 70-71 0.20568585331044176 2307 72-73 0.06255701077609643 2307 74-75 0.1590116729941613 2307 76-77 0.11346034340693478 2307 78-79 0.1083901962029401 2307 80-81 0.11216386085725105 2307 82-83 -0.11511913483644065 2307 84-85 0.1009602360574462 2307 86-87 0.11299639756212088 2307 88-89 0.057749053159831476 2307 90-91 0.15196160699525052 2307 92-93 -0.011330791664640572 2307 94-95 -0.08388967531977443 2307 96-97 0.1371495614359013 2307 98-99 -0.1073671249833339 2307 100-101 -0.054672361055239094 2201 1 0.19918986908666625 2201 2 0.1986923483751255 2201 3 0.06280568526063135 2201 4 0.18892877462754143 2201 5 0.1043438293695047 2201 6 0.11630648763608775 2201 7 0.1015939836954729 2201 8 0.14122337931436135 2201 9 0.23337219716654545 2201 10-11 0.159750653074326 2201 12-13 0.126981749992936 2201 14-15 0.20762932958847813 2201 16-17 0.29887851053077696 2201 18-19 0.27919572793486935 2201 20-21 0.32455244643804804 2201 22-23 0.163913721531614 2201 24-25 0.04897795080316314 2201 26-27 0.17194138229979217 2201 28-29 0.09685129185438512 2201 30-31 0.23865232643861134 2201 32-33 0.3741147069857931 2201 34-35 0.4674231920735963 2201 36-37 0.37049282831612373 2201 38-39 0.3091904400273009 2201 40-41 0.31639916895608877 2201 42-43 0.4281233051261992 2201 44-45 0.28704718104973637 2201 46-47 0.25568623686292113 2201 48-49 0.35023891648196326 2201 50-51 0.29906845256185477 2201 52-53 0.4176286801071143 2201 54-55 0.450154472190178 2201 56-57 0.44524236287578134 2201 58-59 0.5139168901769011 2201 60-61 0.5124054169229311 2201 62-63 0.49517845474460387 2201 64-65 0.5373177380593646 2201 66-67 0.32845501441374836 2201 68-69 0.300758951705717 2201 70-71 0.3802286473716521 2201 72-73 0.2788983356214487 2201 74-75 0.1208175595480796 2201 76-77 0.35380707870941563 2201 78-79 0.2012589306431991 2201 80-81 0.28285501700892723 2201 82-83 0.16252027153671378 2201 84-85 0.3022881508206581 2201 86-87 0.4237555855698787 2201 88-89 0.2315495094248874 2201 90-91 0.25589689875721433 2201 92-93 0.233687443387808 2201 94-95 0.27797667548147587 2201 96-97 0.31651946673712317 2201 98-99 0.37394293220642894 2201 100-101 0.04658553417146649 1101 1 0.17582853562170442 1101 2 0.11695928889262319 1101 3 0.14938107698505476 1101 4 0.3099486999637122 1101 5 0.3464351963980903 1101 6 0.2817959063612392 1101 7 0.41243320976791864 1101 8 0.1761256080432858 1101 9 0.19840080105892355 1101 10-11 0.27702004400527613 1101 12-13 0.31498114821621215 1101 14-15 0.35324051573923754 1101 16-17 0.36073857117229835 1101 18-19 0.30241923975356855 1101 20-21 0.3090346321436357 1101 22-23 0.22608134097721688 1101 24-25 0.050898936206380085 1101 26-27 0.11757613051308624 1101 28-29 0.2243172269203768 1101 30-31 0.2679109486038982 1101 32-33 0.4204846613332691 1101 34-35 0.24778433609793638 1101 36-37 0.3851831996217996 1101 38-39 0.24450929445952596 1101 40-41 0.2848530974271668 1101 42-43 0.3558281036797908 1101 44-45 0.29179838475710795 1101 46-47 0.2509765951561036 1101 48-49 0.2986596528210512 1101 50-51 0.30320546226109 1101 52-53 0.5088765642934234 1101 54-55 0.5635463850253188 1101 56-57 0.5959242157159252 1101 58-59 0.5736897174130036 1101 60-61 0.6301941789274181 1101 62-63 0.5290714965268322 1101 64-65 0.5652251334067486 1101 66-67 0.5127678118770973 1101 68-69 0.44893907997938953 1101 70-71 0.498288537290847 1101 72-73 0.38129099328869387 1101 74-75 0.30112367753613256 1101 76-77 0.39955247420417805 1101 78-79 0.37859793228084015 1101 80-81 0.36090835142047695 1101 82-83 0.2466171313171941 1101 84-85 0.29166859788000465 1101 86-87 0.29639586450529265 1101 88-89 0.40177997225728035 1101 90-91 0.4355745582480637 1101 92-93 0.3612455332105782 1101 94-95 0.2886767868676472 1101 96-97 0.2618159721014912 1101 98-99 0.19742562671299524 1101 100-101 0.23162356288885633 2304 1 -0.08524587624043534 2304 2 -0.10675652293911853 2304 3 -0.08266887713797288 2304 4 -0.0925858527124177 2304 5 -0.03760362137772688 2304 6 -0.10397671968410549 2304 7 -0.08466417281286454 2304 8 -0.034759145295737426 2304 9 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-0.0923738438376347 2304 72-73 -0.025279512593876063 2304 74-75 0.0695811259982051 2304 76-77 -0.06892772679628223 2304 78-79 -0.17614061282687743 2304 80-81 -0.01077667716133135 2304 82-83 -0.027552958878594325 2304 84-85 -0.07209194853183476 2304 86-87 -0.0463714125002852 2304 88-89 -0.11962963236539537 2304 90-91 -0.23404256401927626 2304 92-93 -0.14904536552612768 2304 94-95 -0.23154522298059987 2304 96-97 -0.1906968293005029 2304 98-99 -0.14689208416422517 2304 100-101 -0.35462867827431666 2202 1 -0.1627432543420433 2202 2 -0.1604208711653996 2202 3 -0.18493122109216031 2202 4 -0.15559554109974982 2202 5 -0.15792834442147807 2202 6 -0.08297413825856381 2202 7 -0.1363554254104642 2202 8 -0.09233963943212586 2202 9 -0.07756718878444957 2202 10-11 -0.09284966501600422 2202 12-13 -0.1565454541102156 2202 14-15 -0.14605529917742643 2202 16-17 -0.1986797030051477 2202 18-19 -0.19745590152678716 2202 20-21 -0.08658320680164877 2202 22-23 -0.1522147935053937 2202 24-25 -0.47325480215415894 2202 26-27 -0.2633715326926449 2202 28-29 -0.2176818263248066 2202 30-31 -0.1274769874380297 2202 32-33 -0.1810197776656537 2202 34-35 -0.09938623864665885 2202 36-37 0.008504166513247924 2202 38-39 -0.05984731579633973 2202 40-41 0.03063730609250115 2202 42-43 -0.16143840960969413 2202 44-45 -0.14821333386706925 2202 46-47 -0.2658149121347435 2202 48-49 -0.13693143378639405 2202 50-51 -0.18199724134638018 2202 52-53 -0.12513710429823988 2202 54-55 -0.23446839752325843 2202 56-57 -0.0664917353655099 2202 58-59 -0.033010563392451786 2202 60-61 -0.04470248691047374 2202 62-63 0.15293397025673272 2202 64-65 0.16976182974637055 2202 66-67 0.16965767888626715 2202 68-69 0.04533806402751139 2202 70-71 -0.032235076750225744 2202 72-73 0.05632002746487785 2202 74-75 0.1322946026089582 2202 76-77 -0.00606817677661553 2202 78-79 0.03912263566899554 2202 80-81 0.06417780413756446 2202 82-83 0.11477600444734293 2202 84-85 0.1474513480507369 2202 86-87 0.09407741903616085 2202 88-89 0.10818346931477407 2202 90-91 -0.14100970890183362 2202 92-93 -0.015313436476979803 2202 94-95 0.03705063776027373 2202 96-97 0.12248712210097423 2202 98-99 0.018217173090047112 2202 100-101 -0.07513784440281768 1102 1 -0.04749282090979534 1102 2 -0.09391694102510684 1102 3 -0.09242667404215865 1102 4 0.06643877184365721 1102 5 -0.0493362812839635 1102 6 -0.019778120517088382 1102 7 0.024897326751379012 1102 8 0.03304725868204628 1102 9 0.047681436464174 1102 10-11 0.1871582059009569 1102 12-13 0.03454764607379701 1102 14-15 -0.13707022201053576 1102 16-17 0.06111905679362195 1102 18-19 0.10551058325788176 1102 20-21 -0.003919965047494145 1102 22-23 0.0017653377474573517 1102 24-25 -0.010537953073679773 1102 26-27 0.024712005390895797 1102 28-29 -0.11992556947764399 1102 30-31 0.008035738983778629 1102 32-33 0.05855125735993738 1102 34-35 0.19033264198131405 1102 36-37 -0.017565586829228153 1102 38-39 0.0296444782409111 1102 40-41 -0.053762323761674224 1102 42-43 0.06975913976967263 1102 44-45 0.1483500536054052 1102 46-47 0.18970908884379867 1102 48-49 0.13722377218699222 1102 50-51 0.24267861514765343 1102 52-53 0.3074203055318989 1102 54-55 0.24643399247003117 1102 56-57 0.3206217653843524 1102 58-59 0.3938430907339381 1102 60-61 0.448038187648379 1102 62-63 0.4189363953500731 1102 64-65 0.3753017852863181 1102 66-67 0.46552970203102007 1102 68-69 0.394171253769791 1102 70-71 0.2524900261566927 1102 72-73 0.3180785392233858 1102 74-75 0.23392515878497733 1102 76-77 0.12618184183704528 1102 78-79 0.12327762891489868 1102 80-81 0.10619680070201554 1102 82-83 0.2649164045877441 1102 84-85 0.09766779826718874 1102 86-87 -0.0858341926936319 1102 88-89 0.01722385562788986 1102 90-91 -0.03573885363097418 1102 92-93 0.12101031348313995 1102 94-95 0.05322324484197338 1102 96-97 0.20211899718738735 1102 98-99 0.03299641514201923 1102 100-101 -0.11761420506099896 2305 1 0.11851381142422213 2305 2 0.1457340707257373 2305 3 0.14173642536076514 2305 4 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74-75 -0.3300490734751733 1213 76-77 -0.41001636507594696 1213 78-79 -0.3029296872993541 1213 80-81 -0.2593830919424107 1213 82-83 -0.43736999998873927 1213 84-85 -0.41551932560695803 1213 86-87 -0.46811124429753903 1213 88-89 -0.34898240579003215 1213 90-91 -0.24521089134728413 1213 92-93 -0.2498041728761109 1213 94-95 -0.24926864474221233 1213 96-97 -0.18578836365542983 1213 98-99 -0.17735834691295693 1213 100-101 -0.12325119022608888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2726.0 3 233.0 4 205.0 5 189.0 6 439.0 7 1725.0 8 6149.0 9 10366.0 10 12803.0 11 9712.0 12 7074.0 13 5755.0 14 5130.0 15 5206.0 16 5484.0 17 5778.0 18 6036.0 19 6998.0 20 7854.0 21 9049.0 22 9980.0 23 11103.0 24 12652.0 25 14499.0 26 17523.0 27 20764.0 28 25520.0 29 31444.0 30 39091.0 31 51475.0 32 70476.0 33 100173.0 34 152836.0 35 256655.0 36 500826.0 37 882718.0 38 270967.0 39 1041.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.64865423714453 25.122616799327442 23.918156225792924 14.310572737735114 2 32.907690035064256 22.067158551091968 32.493721865014265 12.531429548829506 3 38.99497104630143 18.537440810975625 26.037068662960607 16.43051947976234 4 31.68771460051028 17.81127397161784 33.817212345443906 16.68379908242797 5 30.876781266809907 16.60048941112089 35.61561949062688 16.907109831442334 6 25.307322360269936 17.18175064407365 40.585056402852075 16.925870592804333 7 33.957793547995905 17.122982048226394 30.1494257966668 18.769798607110904 8 30.73003826515741 26.45892227967998 24.909816882217466 17.90122257294514 9 29.678140658408005 26.825886469425615 24.822853785298086 18.673119086868294 10-11 24.549396579190724 28.14217884258216 30.179841160059993 17.128583418167125 12-13 25.20364447729858 25.874653953048384 29.790430507087283 19.131271062565755 14-15 23.496428894137676 26.795001714869226 30.823729903724423 18.884839487268675 16-17 22.64022174927594 27.9576221649781 31.549019120123 17.853136965622955 18-19 22.189069787278857 28.006933513543025 31.473729145685976 18.330267553492142 20-21 22.189661356199466 28.312384324517353 31.07476245117975 18.423191868103427 22-23 22.30908012777746 27.767862536099347 31.074639336725685 18.84841799939751 24-25 22.256328013180703 27.797445118570778 31.02239101903933 18.923835849209187 26-27 21.755977774945542 28.382787744114164 30.977468480292337 18.883766000647956 28-29 21.293524292549105 28.627125859866904 30.458767792827242 19.620582054756753 30-31 21.26083113734625 28.89631128632326 30.34288130621746 19.499976270113024 32-33 20.758465476928343 29.1215909190422 31.366590349828215 18.753353254201237 34-35 21.490948620954455 28.846364171710565 31.15244093961064 18.510246267724344 36-37 20.998683755463833 29.131724295912324 31.477823624789146 18.391768323834693 38-39 21.2420443895042 29.001197233751718 30.87638989653036 18.880368480213725 40-41 21.29004860462645 29.001610293422164 30.03623452131235 19.672106580639035 42-43 21.338394361392144 28.858530855706565 30.67581977204733 19.12725501085396 44-45 20.817918971076114 28.820320779634063 31.284081984586777 19.07767826470305 46-47 21.057480903874822 28.759081828633814 30.87683520002118 19.306602067470184 48-49 20.893987143102144 29.71143324841362 29.892511381623045 19.502068226861194 50-51 20.9417150191015 29.2079857545764 29.790901759767735 20.059397466554366 52-53 21.228377842415284 28.828991784062012 29.801189370779905 20.1414410027428 54-55 21.955427458775013 28.978419092129638 29.402785092866925 19.66336835622843 56-57 22.023334457178763 29.177045366239945 29.13497902717552 19.664641149405774 58-59 21.610940699078217 29.213882522499514 29.801943540691838 19.37323323773043 60-61 20.881246890430493 29.444327590678792 29.679803602654566 19.994621916236152 62-63 21.137557827541908 29.244432169278 29.401258226171457 20.216751777008636 64-65 20.77669865135137 29.170752301574332 29.612876364796612 20.43967268227768 66-67 21.164622399296718 28.40673231593762 29.759709970033065 20.668935314732597 68-69 21.215209959631267 28.759760219569795 30.09561138620459 19.929418434594346 70-71 20.567682260070384 30.234089721750774 29.46223199222307 19.735996025955767 72-73 20.345238002629348 30.497008922667625 29.04059930454535 20.117153770157685 74-75 20.92050160376197 29.764872993449774 29.033547421114577 20.281077981673683 76-77 20.82265286027899 29.431422123547122 29.291663376127847 20.454261640046045 78-79 20.68301018440003 29.61840938319532 29.474649300293027 20.223931132111623 80-81 19.985311512661283 30.017840189257754 29.48251778182441 20.514330516256557 82-83 19.44750592123339 29.68649180445258 29.67919485236028 21.186807421953745 84-85 19.6162451546474 29.37988245610108 30.04454366826889 20.95932872098263 86-87 19.978452449890266 29.37501399033572 30.617355802218015 20.029177757555995 88-89 20.124098722324245 30.051217971737177 29.884377594108035 19.940305711830543 90-91 20.405000729678456 29.654696696988857 29.4664007394075 20.473901833925183 92-93 19.84724077630882 30.25159438694866 29.64763090333647 20.25353393340605 94-95 19.47525806780996 30.640397154568394 29.80489441464921 20.079450362972434 96-97 19.85742925248592 31.136200854895378 29.264818763658035 19.741551128960662 98-99 20.495271637608965 30.274945516811957 28.757491438356166 20.472291407222915 100-101 20.43298867551013 30.102914700156646 28.19762751849676 21.266469105836464 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2153.0 1 1348.0 2 429.0 3 307.5 4 289.0 5 284.5 6 342.5 7 473.0 8 609.5 9 772.0 10 942.5 11 1032.5 12 1016.0 13 901.5 14 789.5 15 689.5 16 609.5 17 622.5 18 685.0 19 725.5 20 803.0 21 994.5 22 1321.5 23 1742.0 24 2305.5 25 3272.0 26 5136.0 27 7897.0 28 10804.0 29 13668.5 30 16896.0 31 20265.0 32 25934.5 33 36628.5 34 53003.0 35 76564.0 36 110132.0 37 147254.5 38 180442.5 39 199287.5 40 197410.5 41 180734.0 42 162334.5 43 153792.0 44 142412.0 45 128451.5 46 120568.0 47 107348.5 48 89497.0 49 75284.0 50 62452.0 51 51283.0 52 41663.0 53 33151.0 54 27252.0 55 21695.5 56 15592.5 57 10483.0 58 7267.0 59 5414.0 60 4111.5 61 2969.0 62 2140.0 63 1647.0 64 1252.0 65 951.0 66 688.0 67 506.0 68 438.0 69 353.0 70 242.5 71 183.0 72 143.5 73 108.0 74 87.0 75 65.5 76 56.5 77 52.0 78 48.0 79 39.0 80 32.0 81 26.0 82 22.0 83 17.5 84 15.5 85 15.0 86 9.0 87 5.0 88 5.0 89 9.0 90 8.5 91 4.0 92 5.0 93 5.5 94 4.0 95 3.0 96 2.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22496232530614807 2 0.24175403136675178 3 0.22310088906848302 4 0.21557758427458665 5 0.2236050280495173 6 0.2464464018825325 7 0.2277156997410277 8 0.21546124450973259 9 0.20770526018612812 10-11 0.193666928560404 12-13 0.21968825596609706 14-15 0.21606233329481195 16-17 0.21941679651477092 18-19 0.2076858702253191 20-21 0.2009963337462102 22-23 0.1680915702533182 24-25 0.4956655681607536 26-27 0.3502408620931695 28-29 0.48422549128343706 30-31 0.31241104855478863 32-33 0.361487039362396 34-35 0.3636393250121963 36-37 0.37238419733706035 38-39 0.3644537033661748 40-41 0.3706197109034403 42-43 0.4237869834417491 44-45 0.37835630526623576 46-47 0.3955939804254468 48-49 0.39359681446211864 50-51 0.4083913545593941 52-53 0.41329701464407403 54-55 0.37860837475675296 56-57 0.39136696896908235 58-59 0.3894667528097992 60-61 0.34768138726638004 62-63 0.30913414517806576 64-65 0.3363770401147265 66-67 0.34857332546359454 68-69 0.33814152654834656 70-71 0.30919231506049283 72-73 0.29759711849670406 74-75 0.30944438455101 76-77 0.31617270095173683 78-79 0.32573195163057933 80-81 0.33405024481764517 82-83 0.3524319276645878 84-85 0.3810708997794974 86-87 0.3845610927251194 88-89 0.3773868072257852 90-91 0.3501439122891245 92-93 0.32829142645736886 94-95 0.3050622534081734 96-97 0.3211365309188437 98-99 0.35111341032957505 100-101 0.3679245063509878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2578654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.09763478691357 #Duplication Level Percentage of deduplicated Percentage of total 1 87.80148507441305 43.10845247932175 2 5.621707869950279 5.520251197550731 3 1.81218548994693 2.6692206405467522 4 0.9995928738723694 1.9631058342794778 5 0.6353322052698938 1.5596654291352823 6 0.46118931311499983 1.3585982677768678 7 0.31679581842575505 1.0887747776562373 8 0.2979271280712069 1.1702013861723315 9 0.1983202906407787 0.8763351480643954 >10 1.5393944624982006 14.783548915413682 >50 0.16373041971292027 5.5466954159543205 >100 0.13734471100852794 14.121498666268062 >500 0.012576511780815565 4.035271392392134 >1k 0.002258106967964198 1.7426375847700224 >5k 1.5972432618223178E-4 0.45574286469779274 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5942 0.23043029425428926 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5681 0.2203087347119854 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2851 0.11056155653298193 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2722 0.10555894664425704 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2713 0.10520992734969484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.42918902652314E-4 0.0 0.0 2 5.42918902652314E-4 0.0 0.0 3 5.816988242703364E-4 0.0 0.0 4 6.592586675063812E-4 0.0 0.0 5 7.755984323604485E-4 0.0 0.0 6 8.919381972145158E-4 0.0 0.0 7 9.694980404505606E-4 0.0 0.0 8 0.001085837805304628 0.0 0.0 9 0.0011633976485406728 0.0 0.0 10-11 0.0021522856498002447 0.0 0.0 12-13 0.003083003768632783 0.0 0.0 14-15 0.004323961260409501 0.0 0.0 16-17 0.005991497889984465 0.0 0.0 18-19 0.00783354416684053 0.0 0.0 20-21 0.010082779620685831 0.0 0.0 22-23 0.012235065270486074 0.0 0.0 24-25 0.014872099940511599 0.0 0.0 26-27 0.017644864336200203 0.0 0.0 28-29 0.020533968496742876 0.0 0.0 30-31 0.03185770560920542 0.0 0.0 32-33 0.03565813792777162 0.0 0.0 34-35 0.039109550951775617 0.0 0.0 36-37 0.04273547362306071 0.0 0.0 38-39 0.04645834609839086 0.0 0.0 40-41 0.05115071661417158 0.0 0.0 42-43 0.05599820681642438 0.0 0.0 44-45 0.06181519505912775 0.0 0.0 46-47 0.06848534157742761 0.0 0.0 48-49 0.07523304793896352 0.0 0.0 50-51 0.08240733343829765 0.0 0.0 52-53 0.09016331776190215 0.0 3.877992161802243E-5 54-55 0.0990633097732383 0.0 3.877992161802243E-5 56-57 0.1084092708831817 0.0 3.877992161802243E-5 58-59 0.11907374932813786 0.0 3.877992161802243E-5 60-61 0.12944737836095888 0.0 3.877992161802243E-5 62-63 0.14137220425850075 0.0 3.877992161802243E-5 64-65 0.15527480615856182 0.0 3.877992161802243E-5 66-67 0.17057348523687166 0.0 3.877992161802243E-5 68-69 0.1883346893379259 0.0 3.877992161802243E-5 70-71 0.20704600151862174 0.0 3.877992161802243E-5 72-73 0.2277544796626457 0.0 3.877992161802243E-5 74-75 0.250692803299706 0.0 3.877992161802243E-5 76-77 0.27867251674710913 0.0 3.877992161802243E-5 78-79 0.3072145390579737 0.0 3.877992161802243E-5 80-81 0.3419031789452947 0.0 3.877992161802243E-5 82-83 0.38101272989707036 0.0 3.877992161802243E-5 84-85 0.42555146987536907 0.0 3.877992161802243E-5 86-87 0.47755534476513717 0.0 3.877992161802243E-5 88-89 0.532487103737066 0.0 3.877992161802243E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 510 0.0 66.97001 2 GAGCGGC 365 0.0 59.777912 9 ACGCGTA 25 0.0050483937 56.924507 3 AGAGCGG 405 0.0 56.21627 8 TCGGGAA 180 0.0 50.072483 3 CGGGAGA 610 0.0 48.9924 4 TTCGGAG 165 0.0 45.9996 3 ATTCGGA 105 2.1827873E-11 45.178177 2 CACCGAG 85 7.3068804E-9 44.651012 1 GTTCGGA 160 0.0 44.47227 2 GAGAGCG 550 0.0 42.258846 7 GTCGCAA 45 9.685661E-4 42.170403 1 GGTCCGG 90 1.21071935E-8 42.170403 1 CGGAAAG 270 0.0 42.1663 5 CGGAGCC 85 3.6783058E-7 39.069637 1 TTGTGTT 32870 0.0 38.839554 1 GATCCGG 160 0.0 38.546383 1 CGGACCG 50 0.0016259681 37.953358 1 AAGGGGC 525 0.0 37.042507 9 TCGGAAA 270 0.0 36.895515 4 >>END_MODULE