FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578654
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG59420.23043029425428926No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC56810.2203087347119854No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA28510.11056155653298193No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA27220.10555894664425704No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA27130.10520992734969484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5100.066.970012
GAGCGGC3650.059.7779129
ACGCGTA250.005048393756.9245073
AGAGCGG4050.056.216278
TCGGGAA1800.050.0724833
CGGGAGA6100.048.99244
TTCGGAG1650.045.99963
ATTCGGA1052.1827873E-1145.1781772
CACCGAG857.3068804E-944.6510121
GTTCGGA1600.044.472272
GAGAGCG5500.042.2588467
GTCGCAA459.685661E-442.1704031
GGTCCGG901.21071935E-842.1704031
CGGAAAG2700.042.16635
CGGAGCC853.6783058E-739.0696371
TTGTGTT328700.038.8395541
GATCCGG1600.038.5463831
CGGACCG500.001625968137.9533581
AAGGGGC5250.037.0425079
TCGGAAA2700.036.8955154
Produced by FastQC (version 0.11.2)