##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4682624 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.621779796968536 33.0 33.0 33.0 27.0 33.0 2 31.48855299934396 33.0 33.0 33.0 27.0 33.0 3 31.431474105117132 33.0 33.0 33.0 27.0 33.0 4 35.03555292929776 37.0 37.0 37.0 33.0 37.0 5 35.024180673058524 37.0 37.0 37.0 33.0 37.0 6 35.137635863994205 37.0 37.0 37.0 33.0 37.0 7 34.98129873335976 37.0 37.0 37.0 33.0 37.0 8 35.01075294535713 37.0 37.0 37.0 33.0 37.0 9 34.78828921561928 37.0 37.0 37.0 33.0 37.0 10-11 34.75321144725692 37.0 37.0 37.0 33.0 37.0 12-13 34.773204938085996 37.0 37.0 37.0 33.0 37.0 14-15 36.4058030283875 40.0 37.0 40.0 33.0 40.0 16-17 36.3459943612812 40.0 37.0 40.0 33.0 40.0 18-19 36.36516811941339 40.0 37.0 40.0 33.0 40.0 20-21 36.323306654559495 40.0 37.0 40.0 33.0 40.0 22-23 36.52061984904191 40.0 37.0 40.0 33.0 40.0 24-25 36.19690786618784 40.0 37.0 40.0 27.0 40.0 26-27 36.09563580163601 40.0 37.0 40.0 27.0 40.0 28-29 35.918653622413416 40.0 37.0 40.0 27.0 40.0 30-31 35.953377422573325 40.0 37.0 40.0 27.0 40.0 32-33 35.86571321976738 40.0 37.0 40.0 27.0 40.0 34-35 35.76903718940491 38.5 37.0 40.0 27.0 40.0 36-37 35.689441539615395 37.0 37.0 40.0 27.0 40.0 38-39 35.59124136381652 37.0 37.0 40.0 27.0 40.0 40-41 35.50312388951152 37.0 37.0 40.0 27.0 40.0 42-43 35.3810652958683 37.0 37.0 40.0 27.0 40.0 44-45 35.267026244259625 37.0 37.0 40.0 27.0 40.0 46-47 35.15615528814614 37.0 37.0 40.0 27.0 40.0 48-49 35.0277829268376 37.0 37.0 40.0 24.5 40.0 50-51 33.92245896745073 37.0 35.0 38.5 22.0 38.5 52-53 34.38026456960883 37.0 35.0 38.5 24.5 40.0 54-55 35.12728440293306 37.0 37.0 40.0 27.0 40.0 56-57 35.22311603494109 37.0 37.0 40.0 27.0 40.0 58-59 34.96092052233961 37.0 37.0 40.0 27.0 40.0 60-61 34.73203133542219 37.0 33.0 40.0 27.0 40.0 62-63 34.46917625673127 37.0 33.0 40.0 27.0 40.0 64-65 34.22054952522346 37.0 33.0 40.0 24.5 40.0 66-67 33.982647549749885 37.0 33.0 37.0 22.0 40.0 68-69 33.83050507578657 37.0 33.0 37.0 22.0 40.0 70-71 33.50719148067408 37.0 33.0 37.0 22.0 40.0 72-73 33.37379148955799 37.0 33.0 37.0 22.0 38.5 74-75 33.176923237911055 37.0 33.0 37.0 22.0 37.0 76-77 32.92832320083782 37.0 33.0 37.0 22.0 37.0 78-79 32.62649328667004 37.0 33.0 37.0 22.0 37.0 80-81 32.32968747864445 37.0 33.0 37.0 22.0 37.0 82-83 32.104931978309594 37.0 33.0 37.0 22.0 37.0 84-85 31.929350509457947 35.0 33.0 37.0 22.0 37.0 86-87 31.723137817599707 33.0 33.0 37.0 15.0 37.0 88-89 31.60651954972255 33.0 33.0 37.0 15.0 37.0 90-91 31.490813056952682 33.0 33.0 37.0 15.0 37.0 92-93 31.31567396827078 33.0 33.0 37.0 15.0 37.0 94-95 31.178348720717274 33.0 33.0 37.0 15.0 37.0 96-97 31.008586958935844 33.0 33.0 37.0 15.0 37.0 98-99 30.878773845604513 33.0 33.0 37.0 15.0 37.0 100-101 29.614948797938933 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.009007973652664703 2306 2 0.050358491103729364 2306 3 -0.010288070745616551 2306 4 0.12236017439062152 2306 5 0.10565094179573009 2306 6 0.01623239371056684 2306 7 0.2142800578895745 2306 8 0.16326740621222058 2306 9 0.1868302225284424 2306 10-11 0.16063660165019655 2306 12-13 0.025170424854074724 2306 14-15 0.25959725126305244 2306 16-17 0.2512065008555169 2306 18-19 0.23834297437907281 2306 20-21 0.25474416305043235 2306 22-23 0.27377421983489825 2306 24-25 0.23629424303418034 2306 26-27 0.15661432394418284 2306 28-29 0.07414092735940159 2306 30-31 0.3158761367123404 2306 32-33 0.34623328750949867 2306 34-35 0.40471487206507817 2306 36-37 0.2496247539570362 2306 38-39 0.2934053465908164 2306 40-41 0.32159509183580326 2306 42-43 0.29883747909935465 2306 44-45 0.2765626152499294 2306 46-47 0.3928823377546067 2306 48-49 0.23193337966976202 2306 50-51 0.27574511033189 2306 52-53 0.2341045714085439 2306 54-55 0.4224297326084212 2306 56-57 0.4132293703615275 2306 58-59 0.4632915482293356 2306 60-61 0.34966882299010393 2306 62-63 0.45423387888637734 2306 64-65 0.4523813034909949 2306 66-67 0.3488768258616801 2306 68-69 0.34033431027485506 2306 70-71 0.37441611346780235 2306 72-73 0.37852811584510704 2306 74-75 0.30581456750753233 2306 76-77 0.34487582940968053 2306 78-79 0.3501443023917261 2306 80-81 0.2941250343336037 2306 82-83 0.28124016058741574 2306 84-85 0.2190242679205241 2306 86-87 0.2354554998795031 2306 88-89 0.3140527143125773 2306 90-91 0.3450646070727501 2306 92-93 0.39091560013152815 2306 94-95 0.42377991505772883 2306 96-97 0.3475569148021975 2306 98-99 0.3355808040156738 2306 100-101 0.2501768701628926 2307 1 0.16296688167265216 2307 2 0.16819681196353642 2307 3 0.15438803679803925 2307 4 0.2278298959408076 2307 5 0.2558363488999831 2307 6 0.11745049389070061 2307 7 0.30827959908236124 2307 8 0.13940483828613992 2307 9 0.20212925272116422 2307 10-11 0.22689031827125916 2307 12-13 0.17317690986322987 2307 14-15 0.3617005808656302 2307 16-17 0.44745516306034716 2307 18-19 0.34417458365244613 2307 20-21 0.2639168333084143 2307 22-23 0.26104873775759074 2307 24-25 0.3257498734560187 2307 26-27 0.3548654394015571 2307 28-29 0.32594441891055936 2307 30-31 0.3546544730038974 2307 32-33 0.42206648894752874 2307 34-35 0.46100348676877445 2307 36-37 0.5553810180415724 2307 38-39 0.393854273434151 2307 40-41 0.343467512811678 2307 42-43 0.49170726640876694 2307 44-45 0.3623739314896284 2307 46-47 0.358083647663328 2307 48-49 0.4180727972389704 2307 50-51 0.21561480955408285 2307 52-53 0.30493888218025234 2307 54-55 0.3948496603863205 2307 56-57 0.40365960104640664 2307 58-59 0.562431708651701 2307 60-61 0.5504700515948002 2307 62-63 0.5509266749536366 2307 64-65 0.6336202808125435 2307 66-67 0.4438274263621267 2307 68-69 0.2356246353779241 2307 70-71 0.39439878101251935 2307 72-73 0.36348151205481116 2307 74-75 0.36729143684075183 2307 76-77 0.33617963091479197 2307 78-79 0.251207931980737 2307 80-81 0.23356177546458667 2307 82-83 0.21418229482290485 2307 84-85 0.30979614275565126 2307 86-87 0.23126564554843654 2307 88-89 0.21048988080589126 2307 90-91 0.35457113730315015 2307 92-93 0.17608860201197984 2307 94-95 0.0674656514291776 2307 96-97 0.277682495612563 2307 98-99 0.22346132194966373 2307 100-101 0.1880189147095841 2201 1 0.14051244173699473 2201 2 0.14265397129539537 2201 3 0.12034625214962702 2201 4 0.19698483505558073 2201 5 0.1594219970019708 2201 6 0.12196018002601505 2201 7 0.32845001208495006 2201 8 0.2075418940266971 2201 9 0.2501927942008848 2201 10-11 0.2121375108041832 2201 12-13 0.07816893986382922 2201 14-15 0.28904739984506733 2201 16-17 0.41324191022047785 2201 18-19 0.384910482341013 2201 20-21 0.33611070598450254 2201 22-23 0.27316684454934403 2201 24-25 0.296470874871801 2201 26-27 0.299589536591931 2201 28-29 0.38728397079594146 2201 30-31 0.4168114846923956 2201 32-33 0.38323636937053607 2201 34-35 0.43707723491596084 2201 36-37 0.4790247056857311 2201 38-39 0.4359154415697688 2201 40-41 0.463380121155474 2201 42-43 0.3912327222171257 2201 44-45 0.4433146032876749 2201 46-47 0.55804027690818 2201 48-49 0.5570285528372878 2201 50-51 0.3906110810815946 2201 52-53 0.45675849099598054 2201 54-55 0.4643115701452416 2201 56-57 0.6108685247039105 2201 58-59 0.7147420945351044 2201 60-61 0.7338440657825203 2201 62-63 0.5872362848162496 2201 64-65 0.7141484319222329 2201 66-67 0.5847492501605416 2201 68-69 0.44602524572574964 2201 70-71 0.4138400647370162 2201 72-73 0.34761488834347176 2201 74-75 0.3142615492391343 2201 76-77 0.48389355799542244 2201 78-79 0.5327071372656604 2201 80-81 0.4762828416872438 2201 82-83 0.3930609551650903 2201 84-85 0.4823208732255182 2201 86-87 0.5141345192215603 2201 88-89 0.5328932333985001 2201 90-91 0.3691046503509341 2201 92-93 0.2784558912400783 2201 94-95 0.3304174197429326 2201 96-97 0.3871823838622461 2201 98-99 0.21414824159578316 2201 100-101 0.16345466824737898 1101 1 0.10056621379332853 1101 2 -0.022283151656790068 1101 3 0.005735317203239276 1101 4 0.09506342556512948 1101 5 0.045357901099301046 1101 6 0.06702996877402256 1101 7 0.1405130062162101 1101 8 0.02366530969073466 1101 9 0.18725450688889822 1101 10-11 0.05028488918712526 1101 12-13 0.12347362390308803 1101 14-15 0.13584249680041438 1101 16-17 0.1575014491491089 1101 18-19 0.2354011267262166 1101 20-21 0.14249097143479617 1101 22-23 0.12380667590601746 1101 24-25 -0.1992925388714255 1101 26-27 0.09064927127113265 1101 28-29 -0.050923082375589956 1101 30-31 0.14030867549902126 1101 32-33 0.21975968705137916 1101 34-35 0.2629370563556179 1101 36-37 0.2112537856526444 1101 38-39 0.11794189163745727 1101 40-41 0.07839113617183813 1101 42-43 0.17243775367587943 1101 44-45 0.1968988674883576 1101 46-47 0.27852148987110326 1101 48-49 0.2218588438894713 1101 50-51 0.3301614914401867 1101 52-53 0.35863554906127604 1101 54-55 0.41435349357266205 1101 56-57 0.4799301143733743 1101 58-59 0.5636751783781833 1101 60-61 0.49452415334151567 1101 62-63 0.41810776334276767 1101 64-65 0.364297025383145 1101 66-67 0.37377378318912946 1101 68-69 0.29660977268974165 1101 70-71 0.23828706127684995 1101 72-73 0.22136777428854515 1101 74-75 0.23085618530655694 1101 76-77 0.24593238006041673 1101 78-79 0.3165277967206279 1101 80-81 0.2538848594073073 1101 82-83 0.25552675411932313 1101 84-85 0.266482936138722 1101 86-87 0.1664523937631408 1101 88-89 0.2534479607370521 1101 90-91 0.23436156758157267 1101 92-93 0.005305202686898269 1101 94-95 0.2306075589427401 1101 96-97 0.175110406139396 1101 98-99 0.18212537956918595 1101 100-101 -0.016455107357074894 2304 1 0.17780499751703616 2304 2 0.12290723714845697 2304 3 0.16943019311364438 2304 4 0.34534012868791564 2304 5 0.3344669319099367 2304 6 0.17556949737799954 2304 7 0.32392285248146635 2304 8 0.20402394948656877 2304 9 0.3346842623213675 2304 10-11 0.285292610898189 2304 12-13 0.2398515616389787 2304 14-15 0.31946230569256784 2304 16-17 0.36146556858220436 2304 18-19 0.3069269865949593 2304 20-21 0.3475825983896996 2304 22-23 0.14194905865596041 2304 24-25 0.24438200348672723 2304 26-27 0.11268638837589862 2304 28-29 0.20468053729090485 2304 30-31 0.2556648890078961 2304 32-33 0.30670428367667313 2304 34-35 0.3165602280924489 2304 36-37 0.20888352002234 2304 38-39 0.12570366265011046 2304 40-41 0.2712621714694947 2304 42-43 0.14836751607189314 2304 44-45 0.28864447650360603 2304 46-47 0.3116332651010012 2304 48-49 0.24965442174089247 2304 50-51 0.09050325598379771 2304 52-53 0.250584642259021 2304 54-55 0.45312991729297636 2304 56-57 0.465273181647639 2304 58-59 0.4915224038278865 2304 60-61 0.3491386287839049 2304 62-63 0.3703637798850181 2304 64-65 0.29211201509509976 2304 66-67 0.2984869046835925 2304 68-69 0.2854986146333829 2304 70-71 0.2662063789808613 2304 72-73 0.13122792752097467 2304 74-75 0.23480392867190147 2304 76-77 0.22185122768600252 2304 78-79 0.24456608933444102 2304 80-81 0.13813716164144552 2304 82-83 0.17995116233142028 2304 84-85 0.2693056757288801 2304 86-87 0.2649674294657878 2304 88-89 0.23823003228380557 2304 90-91 0.23876276293280085 2304 92-93 0.2838156001989276 2304 94-95 0.27698977808535474 2304 96-97 0.27036274702512486 2304 98-99 0.23124630630595888 2304 100-101 -0.03145927844246188 2202 1 0.008203893495398518 2202 2 -0.01826747530310513 2202 3 0.022545880776917215 2202 4 0.05238729004682341 2202 5 -0.015516312304455937 2202 6 0.14109394791456253 2202 7 0.07813234450213713 2202 8 -0.04996453830308667 2202 9 0.11241106166645665 2202 10-11 0.034402936987710575 2202 12-13 -0.017070785081678252 2202 14-15 0.0841570741392701 2202 16-17 0.07921751826431489 2202 18-19 0.10090162275008652 2202 20-21 0.1313169091022104 2202 22-23 -0.011474278835542862 2202 24-25 -0.04100924896590641 2202 26-27 -0.012849255358624134 2202 28-29 0.0977791110084425 2202 30-31 0.12501845302040948 2202 32-33 0.18534262829945192 2202 34-35 0.3321236389540232 2202 36-37 0.24695493465209495 2202 38-39 0.22218658576979067 2202 40-41 0.0630707023994006 2202 42-43 0.1228325733872282 2202 44-45 0.13005575510469924 2202 46-47 0.028872337816117977 2202 48-49 0.10531183158796154 2202 50-51 0.018893061080824225 2202 52-53 0.1424150098506871 2202 54-55 0.18887271501218805 2202 56-57 0.2537754989991612 2202 58-59 0.249771088283552 2202 60-61 0.25857343406279654 2202 62-63 0.275999276718359 2202 64-65 0.21759451092142257 2202 66-67 0.28566390464001046 2202 68-69 0.28515513491066 2202 70-71 0.19773747741353276 2202 72-73 0.2621105340159957 2202 74-75 0.18558599490042837 2202 76-77 0.18687349053055158 2202 78-79 0.1675034965494433 2202 80-81 0.14557509235329036 2202 82-83 0.1404020248448603 2202 84-85 0.19259794605037683 2202 86-87 0.1959755169434807 2202 88-89 0.22475436176779695 2202 90-91 0.055344729820482996 2202 92-93 0.13510137421765833 2202 94-95 0.30782791997284775 2202 96-97 0.2262359275231134 2202 98-99 0.13847447040771144 2202 100-101 0.003465602330475548 1102 1 -0.2030217113152588 1102 2 -0.24378635710814933 1102 3 -0.252801555392125 1102 4 -0.20460167082246272 1102 5 -0.23545660568907323 1102 6 -0.23997857991596305 1102 7 -0.1789957508151403 1102 8 -0.20789825600947154 1102 9 -0.1858437463498177 1102 10-11 -0.2568283083431595 1102 12-13 -0.21621084208316432 1102 14-15 -0.3682899711443852 1102 16-17 -0.24648392296495558 1102 18-19 -0.1773793929604679 1102 20-21 -0.3229971798846947 1102 22-23 -0.35000372225412235 1102 24-25 -0.26854636804899457 1102 26-27 -0.23370489100670255 1102 28-29 -0.23536850773214013 1102 30-31 -0.11550507306341729 1102 32-33 -0.10795375764690363 1102 34-35 -0.014428820307941237 1102 36-37 -0.13301439517071856 1102 38-39 9.377256299742953E-4 1102 40-41 -0.09741809582316563 1102 42-43 -0.1073094988822092 1102 44-45 -0.11576536356535172 1102 46-47 -0.18874733114085984 1102 48-49 -0.12482254465582088 1102 50-51 0.044386479342733764 1102 52-53 -0.11689233329703796 1102 54-55 0.008832133323544156 1102 56-57 0.12729555932630632 1102 58-59 0.18614089358742802 1102 60-61 0.18433132897314408 1102 62-63 0.20860160477217704 1102 64-65 0.1341949230890478 1102 66-67 0.08130151854724232 1102 68-69 0.13239272225145982 1102 70-71 0.12691234711436294 1102 72-73 0.05377354456549455 1102 74-75 0.044847380049304775 1102 76-77 0.02810739008129559 1102 78-79 0.024518732983473512 1102 80-81 0.060619695366838755 1102 82-83 -0.03453312250434237 1102 84-85 0.038444916094775294 1102 86-87 -0.026440351558470354 1102 88-89 -0.09734000677754295 1102 90-91 -0.11274265146074924 1102 92-93 -0.0016826348226857135 1102 94-95 0.07689951991719468 1102 96-97 0.042903810202229664 1102 98-99 -0.06157765653138725 1102 100-101 -0.09466508757762426 2305 1 -0.047162263562626094 2305 2 -0.05774547179355238 2305 3 -0.09757430544257772 2305 4 -0.025396230356271587 2305 5 -0.06309218380223314 2305 6 -0.0357265278102048 2305 7 0.07365752915199408 2305 8 -0.034025508914147906 2305 9 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-0.501637167610788 1213 72-73 -0.315262139566336 1213 74-75 -0.401553328166365 1213 76-77 -0.5096801070350025 1213 78-79 -0.5140742671900114 1213 80-81 -0.46578671287522155 1213 82-83 -0.47429914558372843 1213 84-85 -0.4803076407600635 1213 86-87 -0.5250211235247839 1213 88-89 -0.3750371258793628 1213 90-91 -0.3652859378519224 1213 92-93 -0.3651831731868924 1213 94-95 -0.456948928212789 1213 96-97 -0.2803025475460359 1213 98-99 -0.2800212070827435 1213 100-101 -0.19948836217669808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5194.0 3 502.0 4 528.0 5 434.0 6 1024.0 7 5070.0 8 18239.0 9 39370.0 10 51313.0 11 39356.0 12 25496.0 13 17827.0 14 13803.0 15 12489.0 16 12037.0 17 12443.0 18 13747.0 19 15141.0 20 17341.0 21 19360.0 22 21442.0 23 23777.0 24 26750.0 25 30642.0 26 35790.0 27 42403.0 28 50955.0 29 62932.0 30 78055.0 31 100828.0 32 135756.0 33 192274.0 34 289289.0 35 482723.0 36 911003.0 37 1460144.0 38 416765.0 39 382.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.99276616685186 24.07101592813215 26.3217130367608 14.614504868255192 2 29.75957584095149 22.78324189833696 34.85891265356734 12.59826960714422 3 38.56361597109552 19.54308060463105 26.55671947841796 15.33658394585547 4 28.202030829935808 18.56407486114372 36.837220909633565 16.3966733992869 5 32.82068658177041 17.54043003507354 33.764778255004714 15.874105128151342 6 25.318295833786504 18.377768148236324 40.901646223397556 15.402289794579616 7 33.05427069439869 18.57717654195647 31.855994516784108 16.51255824686073 8 27.81154498024656 27.96572860215339 27.983984936063237 16.238741481536813 9 31.66553527419218 25.651164164334684 25.15008427661219 17.53321628486094 10-11 25.372104035707093 28.706655622500055 28.824493827456365 17.096746514336484 12-13 26.574186175206737 25.16441945365065 28.849833817428294 19.411560553714313 14-15 24.55878015715688 25.714052728928138 30.095377539930233 19.631789573984744 16-17 23.820517537503388 26.916965003971942 31.258504526133557 18.004012932391113 18-19 23.103614244336974 27.29825380648955 31.306415721348657 18.291716227824814 20-21 22.775396446917163 28.096760556644007 30.645090707003288 18.48275228943554 22-23 23.87992475000813 27.23442802647184 30.254770938477098 18.63087628504293 24-25 22.688386955591756 28.222130186291196 30.25014872364898 18.83933413446807 26-27 22.060274021927412 28.95076212696844 29.887898172172665 19.101065678931484 28-29 21.982485807438746 28.673792046757168 29.640792074872934 19.70293007093115 30-31 22.552862028322355 28.193013922489236 29.700224781598035 19.553899267590378 32-33 22.528273718925494 27.740229070931044 30.498444077246482 19.233053132896984 34-35 21.744733647577117 28.000921829402607 30.17065098842086 20.08369353459942 36-37 20.650716503155873 28.476744734495064 30.29654912184413 20.57598964050494 38-39 21.042186287926302 28.64467697560945 30.415032792882563 19.898103943581685 40-41 21.44620897119766 28.069271232358652 30.771142153356283 19.71337764308741 42-43 21.93851990749167 28.145851094967544 30.932909581720818 18.98271941581996 44-45 21.17456298148152 28.80707291396422 31.01464625781788 19.00371784673638 46-47 21.833004586162424 28.89301627781002 30.088986905440475 19.184992230587085 48-49 21.184097535106577 28.961256647569527 30.118975666081134 19.73567015124276 50-51 20.781077634745092 28.463610345236933 30.378889761194884 20.37642225882309 52-53 22.19695499191937 28.07359323607982 29.57739440222118 20.15205736977963 54-55 22.98986119784199 28.273766418936486 29.118596916281845 19.61777546693968 56-57 23.216724373757533 28.27686408663987 28.674046412348037 19.832365127254562 58-59 22.54993283962308 28.14775668901915 29.18724150116737 20.1150689701904 60-61 21.226108702490016 28.469129729564628 29.00970039325629 21.29506117468906 62-63 21.996519653970818 28.439187787850585 29.098715931431784 20.465576626746813 64-65 21.313090149519994 28.7868288073066 29.683950912033062 20.21613013114035 66-67 21.175259566003927 28.344972007167534 29.32657518626165 21.153193240566885 68-69 21.2596394224046 27.930270285851876 30.646459478982806 20.163630812760715 70-71 21.025990646049898 29.63425793702742 29.632308215419812 19.707443201502873 72-73 20.68596304830144 31.325394510534654 28.3650297472881 19.623612693875806 74-75 21.182016290743732 30.952573782816796 28.130618195094264 19.734791731345208 76-77 21.240434919742462 30.37709000431971 28.50444270551209 19.878032370425732 78-79 21.094589764293833 30.180394763685236 29.003149692016777 19.72186578000415 80-81 20.576822556410637 30.487728823468522 28.862451852158237 20.072996767962604 82-83 19.855627588499722 30.370443964510617 29.187832573616735 20.586095873372923 84-85 19.97127057536789 29.972611739268736 29.304077449159866 20.752040236203513 86-87 19.96630629728528 30.00026051831996 29.844399804922165 20.189033379472594 88-89 20.1256732303048 30.24446407437265 29.586360010977515 20.04350268434504 90-91 20.358032391926884 29.8286091534201 29.286025087024363 20.52733336762865 92-93 19.991852423866398 30.566139749360243 29.076356953410844 20.365650873362515 94-95 19.65945832858868 30.986830413358486 29.105012507138404 20.24869875091443 96-97 20.077455003359436 31.167742496077803 28.81237840066968 19.94242409989308 98-99 20.514937365588573 30.421819037662956 28.429695147973945 20.633548448774526 100-101 20.55554328789989 30.359660746677175 27.93336619159396 21.151429773828976 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3843.0 1 2405.0 2 776.5 3 566.5 4 553.0 5 581.5 6 686.0 7 913.0 8 1185.0 9 1461.0 10 1721.5 11 1846.0 12 1815.5 13 1690.0 14 1547.5 15 1486.5 16 1451.0 17 1407.5 18 1512.0 19 1749.0 20 2015.0 21 2362.5 22 2960.5 23 3818.5 24 5104.5 25 7017.0 26 9912.5 27 13712.0 28 18114.0 29 22561.5 30 27734.5 31 34612.0 32 46900.0 33 68037.0 34 98740.0 35 136777.0 36 187174.0 37 248006.0 38 303254.0 39 326914.0 40 321945.5 41 299277.5 42 275014.5 43 274813.5 44 270822.0 45 253023.0 46 239389.0 47 212581.0 48 172271.0 49 143907.5 50 122598.5 51 104473.5 52 89253.0 53 73132.5 54 57461.0 55 43681.5 56 33274.5 57 25126.0 58 19311.0 59 15006.5 60 11320.0 61 8409.5 62 6232.0 63 4587.5 64 3405.5 65 2443.0 66 1700.5 67 1194.0 68 888.5 69 663.0 70 484.0 71 368.0 72 276.5 73 216.5 74 184.5 75 148.5 76 117.5 77 85.5 78 84.5 79 80.0 80 53.5 81 49.5 82 42.0 83 32.5 84 29.0 85 26.0 86 24.5 87 24.0 88 19.0 89 12.5 90 11.5 91 11.0 92 9.5 93 8.0 94 6.0 95 4.0 96 3.5 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23121651450127106 2 0.24657115326791132 3 0.22816267118607003 4 0.22096585162507176 5 0.22905960418773746 6 0.2522517289451384 7 0.23305309159992346 8 0.21938554109832437 9 0.2117616105841511 10-11 0.19790186015362327 12-13 0.2243079948336659 14-15 0.221029918268048 16-17 0.22379546168985595 18-19 0.21240227701391357 20-21 0.2046608909876172 22-23 0.1723819807014187 24-25 0.5085930452669273 26-27 0.36018266681245387 28-29 0.4979473047590411 30-31 0.3294520337315146 32-33 0.38158092556651996 34-35 0.38411155796408164 36-37 0.3908919443457343 38-39 0.3837271581062242 40-41 0.39002704466555504 42-43 0.44362946928901403 44-45 0.39793927507312143 46-47 0.41570709072520023 48-49 0.41343272489954347 50-51 0.4274206086160239 52-53 0.4322149290654129 54-55 0.3974587752507996 56-57 0.41161750334854985 58-59 0.4091722931416231 60-61 0.36499834280950166 62-63 0.3261419238444086 64-65 0.3543419245277861 66-67 0.36611950906158597 68-69 0.3564988348413197 70-71 0.32654767924992484 72-73 0.31434298376295 74-75 0.3269747902030998 76-77 0.33417160976409804 78-79 0.34459311702156736 80-81 0.3529431361561381 82-83 0.3715331403930787 84-85 0.4007261740425881 86-87 0.402434617855288 88-89 0.39601727578383406 90-91 0.3686821747806358 92-93 0.3460773275838504 94-95 0.322244536396687 96-97 0.3398628632151546 98-99 0.3710953516660744 100-101 0.3874964122679933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4682624.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.82881964941328 #Duplication Level Percentage of deduplicated Percentage of total 1 91.91714429693893 47.63957094455144 2 3.7224266975173266 3.8585796392757326 3 1.2491918240501525 1.9423241326865088 4 0.7114363178020496 1.474916184296204 5 0.4272756049645039 1.1072595135149612 6 0.26640603351035436 0.8284506158594225 7 0.21621415882722364 0.7844287250454042 8 0.19207590990651588 0.7964054154833412 9 0.14442206461796284 0.6736702626432276 >10 0.8821202855473255 9.133261307293115 >50 0.11526859577127534 4.21515112125227 >100 0.12658528466907654 14.175346041123198 >500 0.023344276984510526 8.250697540510055 >1k 0.005963600758142825 4.647120954045671 >5k 1.2504813456699806E-4 0.47281760241931525 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8303 0.1773151122105896 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6930 0.1479939452751278 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6653 0.14207845857365445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015375994314298991 0.0 0.0 2 0.0015589549790886478 0.0 0.0 3 0.0017725104556761337 0.0 0.0 4 0.001921999289287374 0.0 0.0 5 0.0020501325752398654 0.0 0.0 6 0.0023277546948035975 0.0 0.0 7 0.0026907990050023234 0.0 0.0 8 0.0030111322198835525 0.0 0.0 9 0.00335282098242353 0.0 0.0 10-11 0.005242786950222782 0.0 0.0 12-13 0.007367663942268267 0.0 2.13555476587486E-5 14-15 0.009706096410901238 0.0 2.13555476587486E-5 16-17 0.012087239974851708 0.0 4.27110953174972E-5 18-19 0.014585839050925293 0.0 4.27110953174972E-5 20-21 0.017693071235273215 0.0 4.27110953174972E-5 22-23 0.02148368094470109 0.0 4.27110953174972E-5 24-25 0.025733434928792064 0.0 4.27110953174972E-5 26-27 0.030453010961375503 0.0 4.27110953174972E-5 28-29 0.036411208758166363 0.0 4.27110953174972E-5 30-31 0.05666694571248941 0.0 6.406664297624579E-5 32-33 0.06321242106989586 0.0 6.406664297624579E-5 34-35 0.07009958518984227 0.0 6.406664297624579E-5 36-37 0.07722166033403494 0.0 6.406664297624579E-5 38-39 0.08461067982396195 0.0 6.406664297624579E-5 40-41 0.0934411987808545 0.0 6.406664297624579E-5 42-43 0.1033715284421726 0.0 6.406664297624579E-5 44-45 0.11393184675942378 0.0 6.406664297624579E-5 46-47 0.1247697871962387 0.0 6.406664297624579E-5 48-49 0.13668618278982042 0.0 6.406664297624579E-5 50-51 0.1495208669327283 0.0 6.406664297624579E-5 52-53 0.16316706188666869 0.0 6.406664297624579E-5 54-55 0.17748595659185962 0.0 6.406664297624579E-5 56-57 0.19345990624060355 0.0 6.406664297624579E-5 58-59 0.21057637768909054 0.0 7.474441680562009E-5 60-61 0.22919841524751933 0.0 8.54221906349944E-5 62-63 0.24926195227291364 0.0 1.0677773829374299E-4 64-65 0.27024377784763415 0.0 1.2813328595249159E-4 66-67 0.29380962468906324 0.0 1.2813328595249159E-4 68-69 0.3220630142415876 0.0 1.2813328595249159E-4 70-71 0.35122401456960883 0.0 1.2813328595249159E-4 72-73 0.3824031141513818 0.0 1.2813328595249159E-4 74-75 0.4163477571549627 0.0 1.2813328595249159E-4 76-77 0.45416843205860646 0.0 1.2813328595249159E-4 78-79 0.49492549476532816 0.0 1.2813328595249159E-4 80-81 0.5400818002897521 0.0 1.388110597818659E-4 82-83 0.5890714266189214 0.0 1.494888336112402E-4 84-85 0.6458131167482164 0.0 1.494888336112402E-4 86-87 0.7064735498728918 0.0 1.494888336112402E-4 88-89 0.7750675689527923 0.0 1.494888336112402E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1665 0.0 75.46471 9 AGAGCGG 1790 0.0 70.98948 8 CGGGAGA 2165 0.0 68.12665 4 GAGAGCG 2365 0.0 63.95665 7 TCGGGAG 2615 0.0 58.035374 3 TCGGGAA 915 0.0 56.49629 3 CGGAAAG 960 0.0 55.824097 5 AAGCGGC 660 0.0 55.31705 9 GATCCGG 595 0.0 55.004448 1 TTGTGTT 64575 0.0 49.697353 1 AACCGGA 310 0.0 48.957745 2 TCGGAGA 700 0.0 48.1033 4 ATTCGGA 475 0.0 47.92705 2 GATCGGG 4005 0.0 47.253723 1 GTTCGGA 405 0.0 46.842285 2 GGATCGG 1110 0.0 46.14944 1 TTCGGAG 515 0.0 45.12356 3 ATCGGGA 4065 0.0 45.036007 2 ATCCGGA 685 0.0 45.004494 2 CGGAGAG 1425 0.0 44.596878 5 >>END_MODULE