FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4682624
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC83030.1773151122105896No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG69300.1479939452751278No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA66530.14207845857365445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC16650.075.464719
AGAGCGG17900.070.989488
CGGGAGA21650.068.126654
GAGAGCG23650.063.956657
TCGGGAG26150.058.0353743
TCGGGAA9150.056.496293
CGGAAAG9600.055.8240975
AAGCGGC6600.055.317059
GATCCGG5950.055.0044481
TTGTGTT645750.049.6973531
AACCGGA3100.048.9577452
TCGGAGA7000.048.10334
ATTCGGA4750.047.927052
GATCGGG40050.047.2537231
GTTCGGA4050.046.8422852
GGATCGG11100.046.149441
TTCGGAG5150.045.123563
ATCGGGA40650.045.0360072
ATCCGGA6850.045.0044942
CGGAGAG14250.044.5968785
Produced by FastQC (version 0.11.2)