##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2574902 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88432181108252 33.0 33.0 33.0 27.0 33.0 2 31.847559635279325 33.0 33.0 33.0 27.0 33.0 3 31.837409346064433 33.0 33.0 33.0 27.0 33.0 4 35.53405333484537 37.0 37.0 37.0 33.0 37.0 5 35.52348594237761 37.0 37.0 37.0 33.0 37.0 6 35.51282378902187 37.0 37.0 37.0 33.0 37.0 7 35.46518430604349 37.0 37.0 37.0 33.0 37.0 8 35.3875347488953 37.0 37.0 37.0 33.0 37.0 9 35.31416147099967 37.0 37.0 37.0 33.0 37.0 10-11 35.249873781604116 37.0 37.0 37.0 33.0 37.0 12-13 35.27955491121604 37.0 37.0 37.0 33.0 37.0 14-15 36.90012746116163 40.0 37.0 40.0 33.0 40.0 16-17 36.854173867587974 40.0 37.0 40.0 33.0 40.0 18-19 36.86204271075171 40.0 37.0 40.0 33.0 40.0 20-21 36.82383251867449 40.0 37.0 40.0 33.0 40.0 22-23 36.849761272467845 40.0 37.0 40.0 33.0 40.0 24-25 36.63042690556767 40.0 37.0 40.0 33.0 40.0 26-27 36.51679248375278 40.0 37.0 40.0 33.0 40.0 28-29 36.4102989162306 40.0 37.0 40.0 33.0 40.0 30-31 36.461488437229846 40.0 37.0 40.0 33.0 40.0 32-33 36.37247398153406 40.0 37.0 40.0 33.0 40.0 34-35 36.272965922586565 38.5 37.0 40.0 33.0 40.0 36-37 36.197896269450254 37.0 37.0 40.0 33.0 40.0 38-39 36.161270215332465 37.0 37.0 40.0 33.0 40.0 40-41 36.03322009925038 37.0 37.0 40.0 30.0 40.0 42-43 35.917514530650095 37.0 37.0 40.0 27.0 40.0 44-45 35.83989312991329 37.0 37.0 40.0 27.0 40.0 46-47 35.7512027642217 37.0 37.0 40.0 27.0 40.0 48-49 35.61259768332931 37.0 37.0 40.0 27.0 40.0 50-51 34.45197234690873 37.0 35.0 38.5 27.0 38.5 52-53 34.95477886148677 37.0 35.0 38.5 27.0 40.0 54-55 35.762622810499195 37.0 37.0 40.0 27.0 40.0 56-57 35.80571513012922 37.0 37.0 40.0 27.0 40.0 58-59 35.585694523519734 37.0 37.0 40.0 27.0 40.0 60-61 35.345155077746654 37.0 37.0 40.0 27.0 40.0 62-63 35.10319169428584 37.0 35.0 40.0 27.0 40.0 64-65 34.83784314898198 37.0 33.0 40.0 27.0 40.0 66-67 34.599921084375254 37.0 33.0 37.0 27.0 40.0 68-69 34.381627727967896 37.0 33.0 37.0 27.0 40.0 70-71 34.05500578274435 37.0 33.0 37.0 27.0 40.0 72-73 33.811330877835346 37.0 33.0 37.0 27.0 38.5 74-75 33.539714909538304 37.0 33.0 37.0 27.0 37.0 76-77 33.29176158937311 37.0 33.0 37.0 27.0 37.0 78-79 33.04351369489014 37.0 33.0 37.0 27.0 37.0 80-81 32.84815208501139 37.0 33.0 37.0 27.0 37.0 82-83 32.68857766237317 37.0 33.0 37.0 27.0 37.0 84-85 32.530508733924634 37.0 33.0 37.0 22.0 37.0 86-87 32.35634094035423 33.0 33.0 37.0 22.0 37.0 88-89 32.26191657002869 33.0 33.0 37.0 22.0 37.0 90-91 32.16134652891644 33.0 33.0 37.0 22.0 37.0 92-93 31.999508913348933 33.0 33.0 37.0 22.0 37.0 94-95 31.871319180302784 33.0 33.0 37.0 22.0 37.0 96-97 31.707982284374317 33.0 33.0 37.0 22.0 37.0 98-99 31.58421291373419 33.0 33.0 37.0 22.0 37.0 100-101 30.318672710650738 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.22074229710677784 2306 2 0.11633208657737981 2306 3 0.21505804482280055 2306 4 0.35066491966372126 2306 5 0.2658409233848005 2306 6 0.21251175781564058 2306 7 0.3913837239108844 2306 8 0.2674228339421063 2306 9 0.3587350878804543 2306 10-11 0.45828915384370106 2306 12-13 0.3729691496722012 2306 14-15 0.5381736016389027 2306 16-17 0.5096755914102076 2306 18-19 0.5563509761767946 2306 20-21 0.5167214524876158 2306 22-23 0.611243985654454 2306 24-25 0.4207666883477543 2306 26-27 0.577831277546494 2306 28-29 0.4360134396760671 2306 30-31 0.5533498836614115 2306 32-33 0.5269143314061466 2306 34-35 0.6748946450099709 2306 36-37 0.5861571469719138 2306 38-39 0.4110321693865231 2306 40-41 0.4592616881578593 2306 42-43 0.5213763682864823 2306 44-45 0.4223333261303921 2306 46-47 0.31134724502567934 2306 48-49 0.49773332737499487 2306 50-51 0.39630911242539213 2306 52-53 0.5844372137099043 2306 54-55 0.6626735987553829 2306 56-57 0.5440555670917604 2306 58-59 0.570548541309023 2306 60-61 0.49189410995995075 2306 62-63 0.659045665525575 2306 64-65 0.6502356018298698 2306 66-67 0.6148393292982846 2306 68-69 0.4938342499949826 2306 70-71 0.5985978736832749 2306 72-73 0.4775578130497351 2306 74-75 0.5004711270389421 2306 76-77 0.598354067207147 2306 78-79 0.7164916545455142 2306 80-81 0.4738451857405863 2306 82-83 0.45856418698874535 2306 84-85 0.5699270021467626 2306 86-87 0.5228840196930236 2306 88-89 0.4061633331730903 2306 90-91 0.3992711193258671 2306 92-93 0.2173107246485131 2306 94-95 0.27521119477992784 2306 96-97 0.35232511153040846 2306 98-99 0.3144846071010008 2306 100-101 0.08940975502336457 2307 1 0.150407296516601 2307 2 0.06549513839513565 2307 3 0.06525243516746926 2307 4 0.20016880009337257 2307 5 0.1967991534367357 2307 6 0.1595708200205479 2307 7 0.13232410103552894 2307 8 0.16233032331887642 2307 9 0.17927333150651492 2307 10-11 0.19722974490839107 2307 12-13 0.06505097067078225 2307 14-15 0.16699777981406783 2307 16-17 0.19648157730492244 2307 18-19 0.32909169560550566 2307 20-21 0.19432525029215242 2307 22-23 0.22144652785228658 2307 24-25 0.17432454806210274 2307 26-27 0.2174546324213793 2307 28-29 0.1591984535452866 2307 30-31 0.329708587332334 2307 32-33 0.3100737462432548 2307 34-35 0.21380074746511468 2307 36-37 0.2882985760913712 2307 38-39 0.2381084943393148 2307 40-41 0.20839707531555263 2307 42-43 0.32858633199045073 2307 44-45 0.14149866489338336 2307 46-47 0.29747895093927923 2307 48-49 0.21385739784241764 2307 50-51 0.0032726762237942353 2307 52-53 0.14059094069290268 2307 54-55 0.19441197222092654 2307 56-57 0.3168259799222639 2307 58-59 0.26580737186851877 2307 60-61 0.2937533595468196 2307 62-63 0.4175248838329395 2307 64-65 0.3627394748822823 2307 66-67 0.2862609977354964 2307 68-69 0.24308269339677935 2307 70-71 0.18108614386741095 2307 72-73 0.19934483943075776 2307 74-75 0.22777706867019987 2307 76-77 0.17654306478740978 2307 78-79 0.0243275772910323 2307 80-81 0.09644610642047269 2307 82-83 0.1389849849113105 2307 84-85 0.18373941361985402 2307 86-87 0.21206861305941516 2307 88-89 0.19576160542285237 2307 90-91 0.04502367185182976 2307 92-93 0.01915632633642872 2307 94-95 0.012803225882379365 2307 96-97 0.12027800045863657 2307 98-99 -0.054327177306454644 2307 100-101 -0.31741696092328553 2201 1 0.14139438944587468 2201 2 0.20207333597699773 2201 3 0.12091366742126652 2201 4 0.18395209845814975 2201 5 0.18784012787567406 2201 6 0.27379635495780974 2201 7 0.25180226677831996 2201 8 0.23295102198245132 2201 9 0.3822221195807458 2201 10-11 0.34815185347147093 2201 12-13 0.1046351033825772 2201 14-15 0.2557177674050024 2201 16-17 0.29577050465469057 2201 18-19 0.30083982403943565 2201 20-21 0.30477418123400923 2201 22-23 0.2093852335531139 2201 24-25 0.22734558634246582 2201 26-27 0.3271173831540892 2201 28-29 0.21826401754589853 2201 30-31 0.4507256416465282 2201 32-33 0.4953902184738723 2201 34-35 0.47930596825684546 2201 36-37 0.5384132783960283 2201 38-39 0.4717966836756702 2201 40-41 0.31682953157764615 2201 42-43 0.3238602704838911 2201 44-45 0.5088554502898575 2201 46-47 0.5605260319493155 2201 48-49 0.5156469170724023 2201 50-51 0.2579713887822592 2201 52-53 0.42856883294621184 2201 54-55 0.6453299988514516 2201 56-57 0.5441210464985815 2201 58-59 0.5522651524401425 2201 60-61 0.6361474946261296 2201 62-63 0.5669611689477634 2201 64-65 0.6863110025863577 2201 66-67 0.5764121108785005 2201 68-69 0.5170734355737565 2201 70-71 0.5838520613633875 2201 72-73 0.6353629958667355 2201 74-75 0.46204897436123105 2201 76-77 0.5309446126562278 2201 78-79 0.6508780993702032 2201 80-81 0.449664710236263 2201 82-83 0.4414382263739469 2201 84-85 0.4799301541640588 2201 86-87 0.36989898427035683 2201 88-89 0.45745021928019725 2201 90-91 0.29962196886957315 2201 92-93 0.3233770445932862 2201 94-95 0.48230792418674184 2201 96-97 0.5223197831202206 2201 98-99 0.30754022519658264 2201 100-101 0.3193424744638058 1101 1 0.10411499575019079 1101 2 0.08261418444559965 1101 3 0.04937551090807446 1101 4 0.0645850989272887 1101 5 0.008437216989449325 1101 6 0.09073668514121636 1101 7 0.19517793418220464 1101 8 0.2356580568103226 1101 9 0.11939400260913402 1101 10-11 0.08096508649874323 1101 12-13 0.0941794815520467 1101 14-15 0.052837179642786225 1101 16-17 0.060812643251296095 1101 18-19 0.02675129109928065 1101 20-21 0.16493184492947677 1101 22-23 0.07848750551880102 1101 24-25 -0.03958790225302522 1101 26-27 0.04554415303099546 1101 28-29 0.11019736651714851 1101 30-31 0.19343739577382024 1101 32-33 0.264794358053436 1101 34-35 0.11160733880841178 1101 36-37 0.20429092846416808 1101 38-39 0.2526473583400133 1101 40-41 0.1921831997857666 1101 42-43 0.24008851702679124 1101 44-45 0.22650604899860838 1101 46-47 0.26855261082025095 1101 48-49 0.22276663648352013 1101 50-51 0.271452947212218 1101 52-53 0.3635826836711473 1101 54-55 0.4224240832515065 1101 56-57 0.5007612962584247 1101 58-59 0.3822839458632856 1101 60-61 0.3958351831188551 1101 62-63 0.38719034818061715 1101 64-65 0.3737927118104878 1101 66-67 0.39495379150758936 1101 68-69 0.28691655377405567 1101 70-71 0.3685076362801709 1101 72-73 0.3794246371776424 1101 74-75 0.4279859647327484 1101 76-77 0.3190799029629545 1101 78-79 0.30195222867342153 1101 80-81 0.20555545705841638 1101 82-83 0.19463492342284638 1101 84-85 0.2570939110614958 1101 86-87 0.43774896639845906 1101 88-89 0.40349103423897503 1101 90-91 0.29405975789175187 1101 92-93 0.30495153133456654 1101 94-95 0.3396264685202368 1101 96-97 0.22612901172420052 1101 98-99 0.1584692849110816 1101 100-101 0.12434646771716373 2304 1 0.11993895399607268 2304 2 0.12487553949555519 2304 3 0.11284450315613626 2304 4 0.20073025886348006 2304 5 0.12612080079153998 2304 6 0.1395081289046871 2304 7 0.1566491232791165 2304 8 0.167059002714673 2304 9 0.1687531384219696 2304 10-11 0.1619065481728299 2304 12-13 0.12051892968122502 2304 14-15 0.14844995354926027 2304 16-17 0.1321149058656843 2304 18-19 0.21287126423335678 2304 20-21 0.17421947820963624 2304 22-23 0.15656659207743928 2304 24-25 0.06409339374786782 2304 26-27 0.1762549105940323 2304 28-29 0.0751142500701718 2304 30-31 0.20680651535515437 2304 32-33 0.3278330757653549 2304 34-35 0.30163388989564766 2304 36-37 0.35877085598214364 2304 38-39 0.14729918578243684 2304 40-41 0.2723445702276521 2304 42-43 0.16236449441463918 2304 44-45 0.23962330055879733 2304 46-47 0.32163816898776787 2304 48-49 0.27815836121975934 2304 50-51 0.14696367958845258 2304 52-53 0.30043027553300305 2304 54-55 0.42221594231735793 2304 56-57 0.5074550387373691 2304 58-59 0.6304220464985519 2304 60-61 0.43114393133479467 2304 62-63 0.37930810347683774 2304 64-65 0.326760731568136 2304 66-67 0.30221476175918127 2304 68-69 0.3370260463999202 2304 70-71 0.3939428082500598 2304 72-73 0.4640234324089434 2304 74-75 0.37634427497817313 2304 76-77 0.36929434059763366 2304 78-79 0.298178581064775 2304 80-81 0.27534563700412207 2304 82-83 0.24423280913074308 2304 84-85 0.1619988658651792 2304 86-87 0.21965137453658912 2304 88-89 0.20772136754433035 2304 90-91 0.1888812464317553 2304 92-93 0.2229221397357648 2304 94-95 0.21482061678653963 2304 96-97 0.2475168045207745 2304 98-99 0.2851536523025686 2304 100-101 0.08342228698786514 2202 1 0.0369742119809402 2202 2 -0.039101110488758195 2202 3 0.025926153726810952 2202 4 0.09039057905061298 2202 5 0.059354399204231356 2202 6 0.08305620927159652 2202 7 0.001929913002193473 2202 8 0.01008704048292941 2202 9 0.08195721255121668 2202 10-11 -0.062442150571719424 2202 12-13 0.03706508772092576 2202 14-15 0.040224754422780507 2202 16-17 0.11858274452332296 2202 18-19 0.0338285926898223 2202 20-21 0.1166017614208954 2202 22-23 0.13112190533912838 2202 24-25 -0.0963315863632559 2202 26-27 -0.09377421749667292 2202 28-29 -0.02499236539783567 2202 30-31 0.1747285715638185 2202 32-33 0.09068754941536383 2202 34-35 0.10500283567429847 2202 36-37 0.07213174320033744 2202 38-39 0.18252111711114338 2202 40-41 0.055531259419197454 2202 42-43 0.053285698016509286 2202 44-45 0.12395047018951288 2202 46-47 -0.012688881785116735 2202 48-49 0.008812467519859979 2202 50-51 0.02808446132428344 2202 52-53 0.1368937956897156 2202 54-55 0.14197058807349805 2202 56-57 0.34197800682324697 2202 58-59 0.20284746717601365 2202 60-61 0.2805310607757221 2202 62-63 0.2952902811423428 2202 64-65 0.371702657635403 2202 66-67 0.29245138391491565 2202 68-69 0.335710290289839 2202 70-71 0.10467673946466505 2202 72-73 0.2534911633021508 2202 74-75 0.1185162986990278 2202 76-77 0.18915837517100442 2202 78-79 0.22994032342538162 2202 80-81 0.18454261769844038 2202 82-83 0.10797796369345036 2202 84-85 0.22518586408705943 2202 86-87 0.2727839913421377 2202 88-89 0.234980815156554 2202 90-91 0.13688075039843994 2202 92-93 0.30698671281430734 2202 94-95 0.30847919531036183 2202 96-97 0.20393619672940844 2202 98-99 0.06656262464197482 2202 100-101 0.07659212213998501 1102 1 -0.021330885489732054 1102 2 -0.05436487663349254 1102 3 -0.0747852187201623 1102 4 0.012422424074266303 1102 5 -0.07490033984683464 1102 6 -0.06963575541599454 1102 7 0.10880488544056277 1102 8 -0.004839061553951751 1102 9 0.07324318252553752 1102 10-11 0.09853998730675784 1102 12-13 0.02917253087290561 1102 14-15 -0.2297697715190452 1102 16-17 -0.05905308555637134 1102 18-19 0.03194137286629939 1102 20-21 0.015661786910612818 1102 22-23 0.02285190452639796 1102 24-25 0.053561756970623264 1102 26-27 0.10387253883230585 1102 28-29 0.04477590919142216 1102 30-31 0.14909151984343794 1102 32-33 0.10211045466787283 1102 34-35 0.2476653745657984 1102 36-37 0.07708837167614746 1102 38-39 0.0635940142910556 1102 40-41 0.005008107834065356 1102 42-43 0.11621076209615211 1102 44-45 0.08712713320802123 1102 46-47 0.06402073181999413 1102 48-49 0.1089106506938009 1102 50-51 0.22137064924046257 1102 52-53 0.20602309607810554 1102 54-55 0.23703342129586247 1102 56-57 0.3232758251085741 1102 58-59 0.3862344295147082 1102 60-61 0.43928175459983976 1102 62-63 0.44710646941204146 1102 64-65 0.38276976732904444 1102 66-67 0.29325636567229907 1102 68-69 0.2884014167394824 1102 70-71 0.30754981821419847 1102 72-73 0.2332935468863937 1102 74-75 0.26406638505576296 1102 76-77 0.20705329730780875 1102 78-79 0.1659913334838734 1102 80-81 0.29252643314615057 1102 82-83 0.1442415946267701 1102 84-85 0.12600090469968706 1102 86-87 0.009575365946659531 1102 88-89 0.10222132858093147 1102 90-91 0.04009962051930671 1102 92-93 0.15309880126226005 1102 94-95 -0.03409562015884404 1102 96-97 0.024797642923097385 1102 98-99 -0.1376386967854657 1102 100-101 -0.14053564828195064 2305 1 -0.02428860330088156 2305 2 -0.05542366688299083 2305 3 -0.01381457913434403 2305 4 -0.021919782412496147 2305 5 0.004614713439785589 2305 6 0.06562446283080448 2305 7 0.09747507416685863 2305 8 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-1.5157479285065207 1213 58-59 -1.5177563041872517 1213 60-61 -1.2308898190470572 1213 62-63 -1.1432433055028994 1213 64-65 -0.8035229424423491 1213 66-67 -0.7831196775981084 1213 68-69 -0.7723355946142902 1213 70-71 -0.7501196539245001 1213 72-73 -0.845156334269312 1213 74-75 -0.6957219596538877 1213 76-77 -0.7403911582563154 1213 78-79 -0.7058467665450507 1213 80-81 -0.6594454608799509 1213 82-83 -0.6577016680760082 1213 84-85 -0.589110800540837 1213 86-87 -0.7507443924721287 1213 88-89 -0.6439472988674524 1213 90-91 -0.5415471489240922 1213 92-93 -0.5787027452268596 1213 94-95 -0.6833353988758333 1213 96-97 -0.6913300214082163 1213 98-99 -0.6104430818847177 1213 100-101 -0.565503686578996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2780.0 3 240.0 4 254.0 5 235.0 6 511.0 7 1869.0 8 6645.0 9 10358.0 10 12666.0 11 10310.0 12 7463.0 13 5885.0 14 5794.0 15 6280.0 16 6685.0 17 6754.0 18 7470.0 19 8202.0 20 9126.0 21 10214.0 22 11176.0 23 12439.0 24 14189.0 25 16422.0 26 19273.0 27 22803.0 28 27866.0 29 34275.0 30 42511.0 31 55664.0 32 76236.0 33 110132.0 34 167882.0 35 283540.0 36 535748.0 37 825071.0 38 199794.0 39 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.603446665130384 23.225635428279595 28.16266092014873 13.00825698644129 2 32.72018160575962 20.290106714017256 35.48343267899251 11.506279001230611 3 40.3645191689019 16.98131200899309 27.719190076770584 14.934978745334423 4 31.697620888047144 16.020064780845047 37.29584731586072 14.986467015247085 5 30.984865617693067 15.219627792519494 38.74364502689904 15.051861562888396 6 23.165056889855133 16.11561270669877 45.798513668827226 14.920816734618873 7 33.86175481865898 15.998539493618457 33.5640871134446 16.57561857427796 8 30.609951745949704 26.94392052677014 26.60075553123436 15.845372196045798 9 30.520188876551902 27.59581694401537 24.307017410719325 17.5769767687134 10-11 25.213157130313462 27.49793618487114 29.644673586454445 17.644233098360953 12-13 26.06094350807392 24.55327516474979 29.39118378918877 19.99459753798752 14-15 24.130803731544717 25.635872555180246 30.822666777462754 19.410656935812284 16-17 22.482288385026106 27.614939643113196 31.94139228356644 17.96137968829426 18-19 21.532348454185996 28.168179270836575 31.755853233288704 18.543619041688718 20-21 21.630409635731688 28.252601339735932 31.43439148487237 18.68259753966001 22-23 21.699281745968452 27.36859857018314 32.104045104937136 18.828074578911274 24-25 22.1661079306941 27.313387454416084 31.693112461861343 18.827392153028473 26-27 21.732270820682672 27.86281707202049 31.86403777152964 18.540874335767196 28-29 20.995915672122777 28.4560046410422 31.183260870710562 19.36481881612446 30-31 20.80172959426887 28.754582643627487 30.632504660323807 19.811183101779836 32-33 20.019038612442223 28.915126536216047 31.898154338281525 19.1676805130602 34-35 20.322554992304898 29.17550914758224 32.05080326889822 18.45113259121464 36-37 20.25130756971366 30.13113902811002 31.571921792204794 18.045631609971522 38-39 20.736019129348026 29.87839890725081 30.403645148099244 18.98193681530192 40-41 21.202250425858182 29.06035968374901 29.86088925075274 19.876500639640064 42-43 20.878618294922948 29.30964674511921 30.953557649513026 18.85817731044482 44-45 20.187845777758717 29.45888095692959 31.875202251965984 18.478071013345705 46-47 20.44707747230625 29.185502055523006 31.590693067569532 18.77672740460121 48-49 20.793397885161387 30.31058109513498 29.77321461786558 19.122806401838055 50-51 20.506833992825534 29.767200422449413 29.88765232475876 19.838313259966288 52-53 20.390503120124805 29.43882605304212 30.146314352574105 20.02435647425897 54-55 21.425141900718135 29.283645290408884 29.839863745679917 19.451349063193067 56-57 21.847002336272308 29.37185410883095 28.994829708306174 19.78631384659057 58-59 21.131614260906932 29.284695059052616 30.16377283257809 19.41991784746236 60-61 20.151107386801 29.60252949945922 30.35461711601984 19.89174599771994 62-63 20.90146737455551 29.631183004745797 29.730279390640018 19.737070230058677 64-65 20.71307733886616 29.715134611240213 29.58939509021562 19.98239295967801 66-67 21.134424435364053 29.274465998681077 29.48000252560291 20.111107040351964 68-69 21.953606754854 29.213317188735523 29.350204619916703 19.482871436493777 70-71 21.11585869254802 30.60725752692089 29.23165154146181 19.045232239069275 72-73 20.28281728855513 31.201701757659645 28.987613133343178 19.52786782044205 74-75 21.234380168380355 30.200328127015 28.674412833031194 19.890878871573456 76-77 21.541696408811365 29.343184347267727 29.142970359679936 19.972148884240976 78-79 21.465817408773653 29.32453135238104 29.708634089439396 19.50101714940591 80-81 19.80853729586697 30.099881724138605 30.000604044761666 20.090976935232764 82-83 19.149295679124588 29.780985139091655 30.291186337155313 20.778532844628447 84-85 19.101348988288024 29.067560923592424 30.814609731477287 21.016480356642266 86-87 19.164946889995246 29.13751364156878 31.88101393847638 19.816525529959595 88-89 19.55011112326212 29.78288614042136 31.221494266250204 19.44550847006631 90-91 19.87112140861424 29.582208627335426 30.31821506308 20.22845490097033 92-93 19.09909004170147 30.629216202140384 30.39905245718746 19.872641298970684 94-95 18.774662173152244 30.880873364044596 30.777048013478204 19.567416449324952 96-97 19.467379995869653 31.67409609682158 29.77349329987492 19.085030607433843 98-99 20.147505571800416 30.564063426928143 29.0055963560932 20.282834645178237 100-101 19.65431604003904 30.52936742599302 28.64189238314459 21.174424150823352 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2763.0 1 1744.0 2 612.5 3 556.5 4 589.5 5 551.5 6 626.0 7 859.0 8 1053.0 9 1174.5 10 1308.5 11 1355.5 12 1270.5 13 1144.5 14 1051.5 15 977.0 16 909.0 17 920.5 18 996.5 19 1100.5 20 1259.0 21 1446.5 22 1702.5 23 2085.5 24 2653.5 25 3436.5 26 4773.0 27 6900.0 28 9068.5 29 10983.0 30 13421.5 31 16762.5 32 23655.5 33 34927.5 34 51073.0 35 77410.0 36 121522.0 37 178979.5 38 227274.0 39 234539.5 40 211499.0 41 183247.0 42 158295.0 43 147992.5 44 132947.0 45 114158.0 46 109955.0 47 99055.0 48 74890.5 49 57394.0 50 46934.0 51 39553.0 52 33892.5 53 28147.5 54 22489.0 55 16705.0 56 12149.0 57 9291.5 58 7482.0 59 5873.0 60 4531.0 61 3529.0 62 2721.5 63 2029.5 64 1498.5 65 1125.5 66 836.0 67 606.5 68 422.5 69 316.5 70 249.5 71 210.0 72 153.5 73 107.0 74 88.5 75 77.5 76 69.0 77 55.5 78 43.5 79 32.5 80 25.0 81 23.5 82 19.5 83 17.5 84 17.5 85 18.0 86 15.5 87 8.5 88 7.5 89 9.5 90 7.5 91 6.5 92 5.0 93 5.5 94 4.0 95 1.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23014468123447027 2 0.2431548851179579 3 0.22598918327765483 4 0.21841607952457998 5 0.22723194902174917 6 0.2497182417039561 7 0.23030002695248208 8 0.21686262234446202 9 0.20944486430939896 10-11 0.19517247646706556 12-13 0.22148415745531286 14-15 0.2181636427328108 16-17 0.22074626529475683 18-19 0.20901766358486654 20-21 0.20259023450212862 22-23 0.16944334192136246 24-25 0.49978601127343875 26-27 0.35574169424700436 28-29 0.487261262758738 30-31 0.3218957459351851 32-33 0.3724025225037691 34-35 0.37415016183140176 36-37 0.38207279345000317 38-39 0.373955979683887 40-41 0.38011155376010425 42-43 0.4360942668886039 44-45 0.3896070607735751 46-47 0.40659799868111485 48-49 0.4058795247353103 50-51 0.41974413006786276 52-53 0.4233947544411399 54-55 0.38807302180820863 56-57 0.4018211178522522 58-59 0.3993355863640635 60-61 0.35640191354855444 62-63 0.31880824978970074 64-65 0.3449840032746877 66-67 0.3564407499780574 68-69 0.34661513331381155 70-71 0.3190412683667184 72-73 0.3071573209388163 74-75 0.3189635955077125 76-77 0.3261483349657579 78-79 0.3363817341397847 80-81 0.34436262040264054 82-83 0.3629458519198012 84-85 0.391898410114249 86-87 0.39345186729436693 88-89 0.38671374677560544 90-91 0.35851849895646515 92-93 0.33731380844785547 94-95 0.3142449693231043 96-97 0.331352416519153 98-99 0.36133414009542886 100-101 0.3788687880160099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2574902.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.31873838364936 #Duplication Level Percentage of deduplicated Percentage of total 1 87.45612787366466 37.884891234062636 2 5.7348750507841855 4.968551039756759 3 1.8182360415224137 2.362910742072949 4 1.0626247615339566 1.84126256179509 5 0.6570191150131923 1.4230619578156654 6 0.45914862815191715 1.193384358127464 7 0.376845350636906 1.1427125608734319 8 0.25388461861193723 0.8798369098626484 9 0.23971696875960255 0.9345812990236803 >10 1.566300368378505 13.286582567066569 >50 0.1736560021721847 5.179843984814656 >100 0.1720387415870508 16.775966002131636 >500 0.023429046481939983 6.710913160175072 >1k 0.005735628859240003 4.548986938508293 >5k 3.6180384222389865E-4 0.8665146839133155 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5866 0.2278144954642934 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5627 0.2185325888130888 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5601 0.21752284164601215 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5021 0.19499771253430226 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4949 0.19220148961009 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4531 0.17596786207785772 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 4005 0.15553990015930705 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3997 0.15522920872328344 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3756 0.14586962921307295 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3682 0.1429957334298548 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3198 0.12419890155042795 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 2832 0.10998476835234895 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2827 0.10979058620483419 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 2750 0.1068001811331072 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2631 0.10217864602225639 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.718550065206365E-4 0.0 0.0 2 2.718550065206365E-4 0.0 0.0 3 3.1069143602358457E-4 0.0 0.0 4 4.660371540353769E-4 0.0 0.0 5 5.43710013041273E-4 0.0 0.0 6 6.990557310530653E-4 0.0 0.0 7 7.767285900589615E-4 0.0 0.0 8 8.544014490648576E-4 0.0 0.0 9 0.001048583596579598 0.0 0.0 10-11 0.0017864757571356112 0.0 0.0 12-13 0.002602040776697521 0.0 0.0 14-15 0.0037865518765374372 0.0 0.0 16-17 0.0048157172583655605 0.0 3.883642950294807E-5 18-19 0.006427429082737907 1.9418214751474036E-5 7.767285900589614E-5 20-21 0.007204157672796868 3.883642950294807E-5 7.767285900589614E-5 22-23 0.00813623198086762 3.883642950294807E-5 7.767285900589614E-5 24-25 0.009398415939713434 3.883642950294807E-5 7.767285900589614E-5 26-27 0.010815945616571038 3.883642950294807E-5 7.767285900589614E-5 28-29 0.012816021735972865 3.883642950294807E-5 7.767285900589614E-5 30-31 0.020020179408769732 3.883642950294807E-5 7.767285900589614E-5 32-33 0.02227269231994072 3.883642950294807E-5 7.767285900589614E-5 34-35 0.0241562591508337 3.883642950294807E-5 7.767285900589614E-5 36-37 0.02631168098824732 3.883642950294807E-5 7.767285900589614E-5 38-39 0.028253502463394725 3.883642950294807E-5 7.767285900589614E-5 40-41 0.030525433589317183 3.883642950294807E-5 7.767285900589614E-5 42-43 0.033166310795517656 3.883642950294807E-5 7.767285900589614E-5 44-45 0.03607904300823876 3.883642950294807E-5 7.767285900589614E-5 46-47 0.03864224735543333 3.883642950294807E-5 7.767285900589614E-5 48-49 0.041962762077935395 3.883642950294807E-5 7.767285900589614E-5 50-51 0.045691059310218406 3.883642950294807E-5 7.767285900589614E-5 52-53 0.04982713905228238 3.883642950294807E-5 7.767285900589614E-5 54-55 0.054662274525399415 3.883642950294807E-5 1.1650928850884422E-4 56-57 0.05910904570348697 3.883642950294807E-5 1.1650928850884422E-4 58-59 0.06458498226340265 3.883642950294807E-5 1.1650928850884422E-4 60-61 0.07163379421818772 3.883642950294807E-5 1.1650928850884422E-4 62-63 0.0791292251122567 3.883642950294807E-5 1.1650928850884422E-4 64-65 0.08778974889141411 3.883642950294807E-5 1.1650928850884422E-4 66-67 0.09726583769013344 3.883642950294807E-5 1.1650928850884422E-4 68-69 0.10821771080996481 3.883642950294807E-5 1.1650928850884422E-4 70-71 0.12076187753941703 3.883642950294807E-5 1.1650928850884422E-4 72-73 0.1345293917982121 3.883642950294807E-5 1.1650928850884422E-4 74-75 0.14967559930436186 3.883642950294807E-5 1.5534571801179229E-4 76-77 0.16884137726406673 3.883642950294807E-5 1.5534571801179229E-4 78-79 0.19270636319362833 3.883642950294807E-5 1.5534571801179229E-4 80-81 0.21894037132286975 3.883642950294807E-5 1.5534571801179229E-4 82-83 0.25082507994479014 3.883642950294807E-5 1.5534571801179229E-4 84-85 0.28494288326313 3.883642950294807E-5 1.5534571801179229E-4 86-87 0.32624542603951534 3.883642950294807E-5 1.5534571801179229E-4 88-89 0.3751599089984784 3.883642950294807E-5 1.5534571801179229E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGCGT 25 5.321209E-5 75.87094 1 CACGCCG 25 0.0050578257 56.897663 2 GAGCGGC 375 0.0 55.621365 9 CGGGAGA 510 0.0 51.132526 4 AGAGCGG 400 0.0 50.95991 8 TCGGGAA 215 0.0 48.516445 3 TTGTGTT 35235 0.0 48.395042 1 CGAGCGG 55 5.0150757E-5 43.10849 1 GGTACGG 100 6.475602E-10 42.677402 1 CGAGGCG 105 1.0459189E-9 40.645145 1 GAACCGG 105 1.0459189E-9 40.645145 1 CGGAAAG 190 0.0 39.92741 5 TGTGTTT 60670 0.0 38.25905 1 ATCGGGA 820 0.0 38.163067 2 GAGAGCG 635 0.0 38.073704 7 GGTCCGG 75 7.006862E-6 37.93547 1 AAGCGGC 150 0.0 37.923656 9 CCCGTGT 210 0.0 37.345303 38-39 GGAGAGC 640 0.0 35.56035 6 CGACGGG 110 7.124072E-8 34.486794 1 >>END_MODULE