FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34311-1.34200000035761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34311-1.34200000035761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574902
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG58660.2278144954642934No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC56270.2185325888130888No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG56010.21752284164601215No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC50210.19499771253430226No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT49490.19220148961009No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT45310.17596786207785772No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG40050.15553990015930705No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA39970.15522920872328344No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT37560.14586962921307295No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA36820.1429957334298548No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG31980.12419890155042795No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA28320.10998476835234895No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA28270.10979058620483419No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT27500.1068001811331072No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA26310.10217864602225639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGT255.321209E-575.870941
CACGCCG250.005057825756.8976632
GAGCGGC3750.055.6213659
CGGGAGA5100.051.1325264
AGAGCGG4000.050.959918
TCGGGAA2150.048.5164453
TTGTGTT352350.048.3950421
CGAGCGG555.0150757E-543.108491
GGTACGG1006.475602E-1042.6774021
CGAGGCG1051.0459189E-940.6451451
GAACCGG1051.0459189E-940.6451451
CGGAAAG1900.039.927415
TGTGTTT606700.038.259051
ATCGGGA8200.038.1630672
GAGAGCG6350.038.0737047
GGTCCGG757.006862E-637.935471
AAGCGGC1500.037.9236569
CCCGTGT2100.037.34530338-39
GGAGAGC6400.035.560356
CGACGGG1107.124072E-834.4867941
Produced by FastQC (version 0.11.2)