FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5588832
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA269560.48231902479802574No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC230620.41264435932230564No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT134250.24021119260697046No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC108650.19440555736869528No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT102250.1829541485591265No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC84580.15133752454895763No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG71000.12703906648115385No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC70820.1267169956083847No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT70220.12564342603248765No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT67720.12117021946624984No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA62250.11138284349932151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC8200.055.508439
CGGGAGA10850.051.57554
TTGTGTT680700.050.7244641
ATTCGGA2250.050.587512
AGAGCGG10400.042.3997088
TCGGGAA4550.040.6484873
CGGAAAG6050.039.976615
GTTCGGA2950.038.583692
CGGACCG1054.725007E-836.1362041
GGATCGG10000.036.045861
TCGGGAG17600.035.0285263
TGTGTTT1023650.033.8163262
CGGAGAG8700.033.7958685
ATGCCGG1456.7666406E-1032.709491
AACCGGA3500.032.5205422
AAGCGGC3850.032.0194749
GAACCGG3000.031.6191771
GATCCGG5450.031.3290961
GGTCCGG2750.031.0442811
ACGGCGA1401.7334969E-830.489921
Produced by FastQC (version 0.11.2)