##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8114130 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.964694304873102 33.0 33.0 33.0 27.0 33.0 2 30.595568717779972 33.0 33.0 33.0 27.0 33.0 3 30.439179308194472 33.0 33.0 33.0 22.0 33.0 4 33.88278718728933 37.0 37.0 37.0 27.0 37.0 5 33.92269657991676 37.0 37.0 37.0 27.0 37.0 6 34.293606338572346 37.0 37.0 37.0 27.0 37.0 7 33.89578858115411 37.0 37.0 37.0 22.0 37.0 8 34.05168268193879 37.0 37.0 37.0 27.0 37.0 9 33.60126039390545 37.0 37.0 37.0 22.0 37.0 10-11 33.613934827270455 37.0 37.0 37.0 22.0 37.0 12-13 33.619550031858005 37.0 37.0 37.0 22.0 37.0 14-15 35.142644313068686 40.0 37.0 40.0 22.0 40.0 16-17 35.04517514508641 40.0 37.0 40.0 18.5 40.0 18-19 35.09523769030075 40.0 37.0 40.0 18.5 40.0 20-21 35.07937492990622 40.0 37.0 40.0 18.5 40.0 22-23 35.63472189871249 40.0 37.0 40.0 27.0 40.0 24-25 35.10470087366113 38.5 37.0 40.0 22.0 40.0 26-27 34.916971320400336 37.0 37.0 40.0 18.5 40.0 28-29 34.656063250157445 37.0 37.0 40.0 15.0 40.0 30-31 34.73973395792278 37.0 37.0 40.0 18.5 40.0 32-33 34.55654475587648 37.0 37.0 40.0 15.0 40.0 34-35 34.546339472007475 37.0 37.0 40.0 15.0 40.0 36-37 34.4171927859179 37.0 37.0 40.0 15.0 40.0 38-39 34.527442313593696 37.0 37.0 40.0 18.5 40.0 40-41 34.225218168799366 37.0 35.0 40.0 15.0 40.0 42-43 34.07398907831154 37.0 33.0 40.0 15.0 40.0 44-45 33.98751677629025 37.0 33.0 40.0 15.0 40.0 46-47 33.90899880825178 37.0 33.0 40.0 15.0 40.0 48-49 33.793265390128084 37.0 33.0 40.0 15.0 40.0 50-51 32.773791583324396 37.0 33.0 38.5 15.0 38.5 52-53 33.23369393884495 37.0 33.0 38.5 15.0 40.0 54-55 33.948177253753634 37.0 33.0 40.0 15.0 40.0 56-57 34.14604332195812 37.0 33.0 40.0 18.5 40.0 58-59 33.737344792356055 37.0 33.0 40.0 15.0 40.0 60-61 33.522087210828516 37.0 33.0 40.0 15.0 40.0 62-63 33.218663738441464 37.0 33.0 40.0 15.0 40.0 64-65 32.99791635085955 37.0 33.0 38.5 15.0 40.0 66-67 32.827869654540905 37.0 33.0 37.0 15.0 40.0 68-69 32.82230812175797 37.0 33.0 37.0 15.0 40.0 70-71 32.47336411913538 37.0 33.0 37.0 15.0 40.0 72-73 32.56977353086529 37.0 33.0 37.0 18.5 37.0 74-75 32.533470439837664 37.0 33.0 37.0 22.0 37.0 76-77 32.24460761658983 37.0 33.0 37.0 22.0 37.0 78-79 31.838007155419003 37.0 33.0 37.0 18.5 37.0 80-81 31.426335232489496 35.0 33.0 37.0 15.0 37.0 82-83 31.130463339877473 33.0 33.0 37.0 15.0 37.0 84-85 30.93867574219294 33.0 33.0 37.0 15.0 37.0 86-87 30.734415088247292 33.0 33.0 37.0 15.0 37.0 88-89 30.619536598501625 33.0 27.0 37.0 15.0 37.0 90-91 30.49541922547457 33.0 27.0 37.0 15.0 37.0 92-93 30.304091319710185 33.0 27.0 37.0 15.0 37.0 94-95 30.161534200216167 33.0 27.0 37.0 15.0 37.0 96-97 29.999170212949508 33.0 27.0 37.0 15.0 37.0 98-99 29.872445844471308 33.0 27.0 37.0 15.0 37.0 100-101 28.637052093077138 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19379003825872942 2306 2 0.26335832522716274 2306 3 0.25927495128805234 2306 4 0.37254689866358603 2306 5 0.31279453932108936 2306 6 0.1352289230659096 2306 7 0.5194999377244898 2306 8 0.1760007569027664 2306 9 0.4917598214696355 2306 10-11 0.437121148653695 2306 12-13 0.39858470629860676 2306 14-15 0.6841848548904537 2306 16-17 0.6610533535654 2306 18-19 0.6200267145725675 2306 20-21 0.6106731647176531 2306 22-23 0.353395368246872 2306 24-25 0.4685352827158198 2306 26-27 0.3403921373054217 2306 28-29 0.48413371869538224 2306 30-31 0.5530409213571872 2306 32-33 0.6108410429046955 2306 34-35 0.57236640521716 2306 36-37 0.649729272746491 2306 38-39 0.48693503722478226 2306 40-41 0.6248995481407817 2306 42-43 0.65605581072316 2306 44-45 0.7160050957890576 2306 46-47 0.6345627965188072 2306 48-49 0.6668845914291452 2306 50-51 0.6245452256527599 2306 52-53 0.6533057771436788 2306 54-55 0.7247812195659478 2306 56-57 0.6719720657643151 2306 58-59 0.8527215128250631 2306 60-61 0.7357777598543578 2306 62-63 0.8522013906084709 2306 64-65 0.7492276834994698 2306 66-67 0.6528273659867168 2306 68-69 0.49243877315469575 2306 70-71 0.5997326188708598 2306 72-73 0.37256511090251365 2306 74-75 0.4408107134514765 2306 76-77 0.44056329541862027 2306 78-79 0.5728998132084904 2306 80-81 0.5955871805302557 2306 82-83 0.6219026201416789 2306 84-85 0.5489296905801204 2306 86-87 0.5421101953265222 2306 88-89 0.5554915887562011 2306 90-91 0.4608102087728341 2306 92-93 0.3738210218424385 2306 94-95 0.554778284200502 2306 96-97 0.6382450013700804 2306 98-99 0.5408876974478751 2306 100-101 0.2731999885399645 2307 1 -0.025016499371595557 2307 2 -0.08792969052678146 2307 3 -0.0613368692138998 2307 4 0.011160286432968292 2307 5 -0.015503112185172085 2307 6 -0.15706548577097834 2307 7 0.1656028923633741 2307 8 -0.1519820239855818 2307 9 0.08219917141276767 2307 10-11 0.05289923137079455 2307 12-13 -0.029963680678832816 2307 14-15 0.11999430344486228 2307 16-17 0.16288516638524442 2307 18-19 0.12804670966782083 2307 20-21 0.12473457794804688 2307 22-23 0.05190199936802031 2307 24-25 0.13882586404636754 2307 26-27 -0.0781305159352641 2307 28-29 -0.07304530578222312 2307 30-31 0.13693369426692215 2307 32-33 0.3049240762074561 2307 34-35 0.20453658400732877 2307 36-37 0.2644506821102155 2307 38-39 0.13790734574119057 2307 40-41 0.17667826407080156 2307 42-43 0.28038229635330936 2307 44-45 0.1357888962037066 2307 46-47 0.2508985545124105 2307 48-49 0.17645395485096316 2307 50-51 0.08789367391401015 2307 52-53 0.07879621978887741 2307 54-55 0.15270350773315045 2307 56-57 0.24825781780767642 2307 58-59 0.32595820748547766 2307 60-61 0.37867059929527613 2307 62-63 0.43555585285584186 2307 64-65 0.43850797947386155 2307 66-67 0.22063787664966128 2307 68-69 0.02683059820853373 2307 70-71 0.16656284752014017 2307 72-73 -0.09480365036943539 2307 74-75 0.021954308738386885 2307 76-77 0.1000577415448376 2307 78-79 0.06906759733149315 2307 80-81 0.19154955746109437 2307 82-83 0.1169830264018934 2307 84-85 0.16499999258102704 2307 86-87 0.12815522913819422 2307 88-89 0.23439228081137387 2307 90-91 0.23127866283465082 2307 92-93 0.09120951766238505 2307 94-95 0.11444684965224283 2307 96-97 0.09777457982932702 2307 98-99 0.1570400109373331 2307 100-101 -0.01724371239435385 2201 1 0.09230418913016791 2201 2 0.036990796374457346 2201 3 -0.007334931186438354 2201 4 0.12467423356945062 2201 5 0.061428744585903416 2201 6 0.005580357472027231 2201 7 0.24300738095647745 2201 8 -0.14308088902194527 2201 9 0.1543211004845091 2201 10-11 0.07333437169328505 2201 12-13 0.010149808040210928 2201 14-15 0.27459098237748236 2201 16-17 0.2563001057300127 2201 18-19 0.25510123869468515 2201 20-21 0.22261044358391757 2201 22-23 0.02728395147327234 2201 24-25 0.18324261619551407 2201 26-27 -0.010917120743648923 2201 28-29 0.09829689163390043 2201 30-31 0.2355901019154416 2201 32-33 0.3159549466822753 2201 34-35 0.3456083657903122 2201 36-37 0.2802843450651977 2201 38-39 0.14105418126383285 2201 40-41 0.25854765429139803 2201 42-43 0.19470933739167862 2201 44-45 0.2679346589461602 2201 46-47 0.24697447701597497 2201 48-49 0.21963500171096229 2201 50-51 0.22953989180698642 2201 52-53 0.3234253109642893 2201 54-55 0.3094252842844867 2201 56-57 0.32181572248124724 2201 58-59 0.47321018484583277 2201 60-61 0.44912885008304215 2201 62-63 0.408950990438413 2201 64-65 0.4289800409880158 2201 66-67 0.3497461638448769 2201 68-69 0.22077776884184885 2201 70-71 0.24312007980124406 2201 72-73 0.18348771029188526 2201 74-75 0.13387513981201948 2201 76-77 0.3017001059098021 2201 78-79 0.2383551446768557 2201 80-81 0.23803334438285972 2201 82-83 0.22143530888461171 2201 84-85 0.2686325785358257 2201 86-87 0.23486275054091976 2201 88-89 0.2414327544757633 2201 90-91 0.15397297154429168 2201 92-93 0.1008303159293007 2201 94-95 0.09165441129250951 2201 96-97 0.207418078286409 2201 98-99 0.22999433977542694 2201 100-101 -0.06971362967320971 1101 1 0.04518043964090879 1101 2 0.032327699841374624 1101 3 0.03731627024097506 1101 4 0.2037919499196974 1101 5 0.14519742894670884 1101 6 0.10918963414410854 1101 7 0.37191240764551736 1101 8 0.1309828491099836 1101 9 0.24032168471315174 1101 10-11 0.18159029129903814 1101 12-13 0.18570808908072678 1101 14-15 0.32193851454923816 1101 16-17 0.336615525121168 1101 18-19 0.2640546627653819 1101 20-21 0.22922013365554506 1101 22-23 0.0877605062955098 1101 24-25 0.06475472028935769 1101 26-27 0.10269294657079087 1101 28-29 0.21103330485677674 1101 30-31 0.303396758163025 1101 32-33 0.3856217521404304 1101 34-35 0.2560529688209101 1101 36-37 0.327075898899416 1101 38-39 0.2618595403316064 1101 40-41 0.3684344139380684 1101 42-43 0.21496242533067544 1101 44-45 0.17032496189490587 1101 46-47 0.2569047277136818 1101 48-49 0.24759836304630056 1101 50-51 0.2613809248641914 1101 52-53 0.35438765544888184 1101 54-55 0.4757457846112132 1101 56-57 0.46147322215887243 1101 58-59 0.5651320968410474 1101 60-61 0.5443057943567169 1101 62-63 0.43031579477044346 1101 64-65 0.5231364044175919 1101 66-67 0.43824702010713423 1101 68-69 0.4212778644397801 1101 70-71 0.41141471456623435 1101 72-73 0.2562209838293228 1101 74-75 0.28919249576207307 1101 76-77 0.29824615442000635 1101 78-79 0.3358787177762075 1101 80-81 0.31221176570462106 1101 82-83 0.25113370113453115 1101 84-85 0.2631076589416388 1101 86-87 0.24257249760515975 1101 88-89 0.18525086371004207 1101 90-91 0.2463111893540173 1101 92-93 0.22670108377576526 1101 94-95 0.3085406328218028 1101 96-97 0.3220230620336153 1101 98-99 0.1886394648130576 1101 100-101 0.08489813231620502 2304 1 0.017152355075911174 2304 2 -0.05260095284122457 2304 3 0.01954437997031988 2304 4 0.10657535561342257 2304 5 0.02768918628191841 2304 6 -0.08000588197836578 2304 7 0.2667376253743896 2304 8 -0.031304179041775626 2304 9 0.152828601553459 2304 10-11 0.0581942628271932 2304 12-13 0.03789329453088186 2304 14-15 0.1323631695794134 2304 16-17 0.19889982833975495 2304 18-19 0.17492426635262603 2304 20-21 0.1568575618925152 2304 22-23 -0.03252829326924456 2304 24-25 0.09109753690620437 2304 26-27 -0.07055962552356476 2304 28-29 0.02948403378156428 2304 30-31 0.14343087762924966 2304 32-33 0.18745465160040453 2304 34-35 0.07943576485212134 2304 36-37 0.1488752300728251 2304 38-39 -0.04412123381984401 2304 40-41 0.12338236100541877 2304 42-43 0.09632446046765608 2304 44-45 0.10549066828068021 2304 46-47 0.07947232489342326 2304 48-49 0.06520606577178256 2304 50-51 0.0914185341027931 2304 52-53 0.05999372032437833 2304 54-55 0.25777668762412986 2304 56-57 0.23723931802429377 2304 58-59 0.3205453056036447 2304 60-61 0.2394107202824003 2304 62-63 0.24143267572110005 2304 64-65 0.19565745581654426 2304 66-67 0.2664108765316442 2304 68-69 0.20104907533505667 2304 70-71 0.21236861664472428 2304 72-73 0.1329713895501925 2304 74-75 0.19296371037316362 2304 76-77 0.20924621418274114 2304 78-79 0.1517238222531354 2304 80-81 0.1757465758485104 2304 82-83 0.1687653605641266 2304 84-85 0.2528391714828828 2304 86-87 0.16139778098014546 2304 88-89 0.13721493670481166 2304 90-91 0.07262895516480228 2304 92-93 0.17786118794665384 2304 94-95 0.18859163448762217 2304 96-97 0.2263486973275306 2304 98-99 0.172461784307135 2304 100-101 0.11353520847942633 2202 1 0.03250898516371947 2202 2 -0.03704779888888865 2202 3 0.01997523593543349 2202 4 0.1634704902499422 2202 5 0.13565518233274787 2202 6 0.07746617325121719 2202 7 0.32260257488159283 2202 8 0.038709473472785305 2202 9 0.2040445668792259 2202 10-11 0.1376722042219498 2202 12-13 0.05250488051983382 2202 14-15 0.2111807640923402 2202 16-17 0.2264288679697657 2202 18-19 0.1262151600443886 2202 20-21 0.22178535803200816 2202 22-23 0.09955401798676888 2202 24-25 0.05122866737490739 2202 26-27 0.054631725794500596 2202 28-29 0.09668567288797902 2202 30-31 0.2189499526551728 2202 32-33 0.23062681600009682 2202 34-35 0.22965530361082642 2202 36-37 0.3583533318376695 2202 38-39 0.21086687294242523 2202 40-41 0.17099492506391556 2202 42-43 0.23187598289401734 2202 44-45 0.2310488497319696 2202 46-47 0.21215875949572904 2202 48-49 0.24514250206146215 2202 50-51 0.05931428429349239 2202 52-53 0.1098280869731596 2202 54-55 0.2395436704830871 2202 56-57 0.39134315951967835 2202 58-59 0.424126993926194 2202 60-61 0.35774823367250264 2202 62-63 0.36656761549649275 2202 64-65 0.4344750119760121 2202 66-67 0.408713312376598 2202 68-69 0.38731228093313774 2202 70-71 0.39143266219855377 2202 72-73 0.23597539773082588 2202 74-75 0.2514914614210255 2202 76-77 0.24426603173346706 2202 78-79 0.28284541701844645 2202 80-81 0.26254271400886964 2202 82-83 0.2445008882115225 2202 84-85 0.2560237586424208 2202 86-87 0.23905096266541648 2202 88-89 0.354713526510249 2202 90-91 0.2387748702518664 2202 92-93 0.3011992024697925 2202 94-95 0.34470733099890083 2202 96-97 0.24517488639890317 2202 98-99 0.18141256928301175 2202 100-101 0.1493439791968072 1102 1 -0.18072830464744172 1102 2 -0.30666000293505036 1102 3 -0.26999648083018357 1102 4 -0.21303154319539175 1102 5 -0.20526440505960153 1102 6 -0.18879585234126495 1102 7 -0.0068841929511336275 1102 8 -0.1943608264787713 1102 9 -0.08193172615344935 1102 10-11 -0.10387451997508634 1102 12-13 -0.17698144128296178 1102 14-15 -0.2735539552670758 1102 16-17 -0.15517163654115507 1102 18-19 -0.12054548376610086 1102 20-21 -0.20073749857584744 1102 22-23 -0.30856137397318406 1102 24-25 -0.2855035416872198 1102 26-27 -0.23784663660423888 1102 28-29 -0.24423198227548681 1102 30-31 -0.040202228210709734 1102 32-33 -0.11508613755850661 1102 34-35 -0.06466422606662547 1102 36-37 -0.21035940008490428 1102 38-39 -0.20680286310813045 1102 40-41 -0.12114538240017225 1102 42-43 -0.10437354347582328 1102 44-45 -0.028126185911695245 1102 46-47 -0.10446259853159034 1102 48-49 -0.16297187696793003 1102 50-51 0.008585419468424504 1102 52-53 -0.06946284392925861 1102 54-55 0.08394053783445088 1102 56-57 0.20383552317237275 1102 58-59 0.20629877084456893 1102 60-61 0.05196041484175851 1102 62-63 0.11524956726830737 1102 64-65 0.07297815732969326 1102 66-67 0.08542956956991787 1102 68-69 0.05134039968121584 1102 70-71 0.0642536055440246 1102 72-73 0.051969215483651965 1102 74-75 0.11311425142572773 1102 76-77 0.11716792270117793 1102 78-79 0.18479470910170548 1102 80-81 0.14100681948657012 1102 82-83 0.15928990081924255 1102 84-85 0.07509718322087977 1102 86-87 -0.07318404852865967 1102 88-89 -0.05368181732545807 1102 90-91 -0.05828541118655295 1102 92-93 -0.011041503266675079 1102 94-95 0.015055778941583497 1102 96-97 0.04790899197347187 1102 98-99 -0.08751563424990749 1102 100-101 -0.15616805013671353 2305 1 -0.12140013795723803 2305 2 -0.22589506920964553 2305 3 -0.10997058993797992 2305 4 -0.13197615909242444 2305 5 -0.11441219328335706 2305 6 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-0.3283798110188165 1213 90-91 -0.28814141326299136 1213 92-93 -0.2610611134920475 1213 94-95 -0.23830125859451812 1213 96-97 -0.214360244105805 1213 98-99 -0.15567630445462655 1213 100-101 -0.10483507944435999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8607.0 3 1112.0 4 1172.0 5 839.0 6 2049.0 7 11906.0 8 64485.0 9 165986.0 10 185057.0 11 117644.0 12 68770.0 13 44296.0 14 31643.0 15 25066.0 16 23063.0 17 23350.0 18 24466.0 19 26269.0 20 28922.0 21 32592.0 22 35156.0 23 38834.0 24 44331.0 25 50626.0 26 59612.0 27 71496.0 28 87206.0 29 106245.0 30 131413.0 31 172740.0 32 233931.0 33 332004.0 34 500612.0 35 843677.0 36 1590519.0 37 2353882.0 38 574279.0 39 273.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.89922905761328 23.680767548036602 25.582295326740784 13.837708067609327 2 29.953038546738647 24.650445221459332 33.47924290917636 11.917273322625665 3 36.72161856476523 20.429907643311708 27.853793012472284 14.994680779450775 4 26.733894762739553 18.41849668890367 36.995959101433 17.851649446923776 5 33.49723386965386 17.191016218322037 33.167563553556974 16.14418635846712 6 27.411202193387396 17.61616140650769 40.1277243914646 14.844912008640318 7 34.34321986530976 18.72890022321558 31.076868499452985 15.851011412021673 8 26.909990693952057 30.17912444106592 27.387606468413473 15.523278396568557 9 32.79197567737423 26.666317586913756 23.840611666764023 16.701095068947986 10-11 26.57731696660394 29.326769947468506 27.32507596467591 16.770837121251645 12-13 28.633175042166513 24.18228581535266 27.12076321637375 20.063775926107073 14-15 25.882108107707545 24.77684845999748 28.438470297922375 20.9025731343726 16-17 26.065323764198624 26.24443163372401 29.58626347685903 18.103981125218343 18-19 25.623978457375703 26.63224836012886 29.761634394101343 17.982138788394103 20-21 24.698870202553223 28.289317254307505 28.962720567909944 18.049091975229327 22-23 27.143219634449228 26.6325094539897 28.196050936708346 18.02821997485273 24-25 24.46943583951669 28.980625335063735 28.381840850475605 18.16809797494397 26-27 22.273305899895778 30.955546131572408 28.354359822764806 18.416788145767004 28-29 23.030906950974188 29.749076533880075 28.072051162276914 19.147965352868827 30-31 25.07916838516523 27.636869137941776 28.184278927894272 19.099683548998723 32-33 23.082170125716225 27.03044184613375 29.72898538641962 20.158402641730405 34-35 20.517780121695424 27.284943460030647 33.08847131859964 19.108805099674296 36-37 21.176777815982344 27.95060242089134 30.183565631747587 20.68905413137873 38-39 20.62757407172611 27.570806106955214 27.81111980431025 23.990500017008433 40-41 21.88617978906908 27.043436588607513 28.601098198566415 22.469285423756993 42-43 23.725793131275374 27.19395107153057 30.22460384822106 18.855651948972994 44-45 21.984387442999964 28.883552108477073 30.58197037798204 18.550090070540925 46-47 23.271567072553935 29.42968214210478 28.835622307373686 18.4631284779676 48-49 21.25856804671055 29.07062955995294 29.965458070334076 19.70534432300243 50-51 20.184965010842166 28.02626746944192 30.583732199523123 21.205035320192792 52-53 23.78677912670336 27.594575825411216 28.245572316109087 20.37307273177634 54-55 25.35368663480448 27.491382066186127 27.8756879748771 19.279243324132292 56-57 25.76686782567929 27.115443851369708 27.228112860296307 19.889575462654697 58-59 24.36486749239187 26.79675283407546 27.97911908718665 20.859260586346018 60-61 21.372182537584457 27.116935028811945 27.9161561153764 23.5947263182272 62-63 23.210253171786963 27.254403635312556 28.06602158231934 21.469321610581137 64-65 21.50276420184402 28.348633400199823 29.757572223819878 20.391030174136276 66-67 20.612425579764686 28.393994671876925 28.702024274924277 22.291555473434116 68-69 20.233423553489434 27.31298825839483 32.188289855330545 20.265298332785186 70-71 19.810167673538693 30.904304412675295 30.08104737191164 19.20448054187437 72-73 19.75153267870641 34.06785506567387 27.221220592625468 18.959391662994253 74-75 20.59130988218421 33.618877132221186 26.72934870137934 19.06046428421526 76-77 20.7517910509673 32.67197560452858 27.291487815030024 19.2847455294741 78-79 20.706919889856295 32.411209343889695 27.780749532628697 19.101121233625314 80-81 19.96103339471832 32.90622911686247 27.55347443471155 19.579263053707656 82-83 19.262734384667564 32.340942289952125 28.066532540715045 20.329790784665263 84-85 19.423782473708336 31.57158613539596 28.38922612408812 20.61540526680758 86-87 19.347084668966247 31.45141859324736 29.022124013419397 20.179372724367 88-89 19.621253082218495 31.657676998038102 28.637627106042174 20.08344281370123 90-91 20.15383652585691 30.88540692044835 28.255815636107595 20.70494091758714 92-93 19.77765913937975 31.65070561166149 27.894738957226185 20.67689629173258 94-95 19.495225747430524 32.206676690613904 27.796339765452 20.50175779650357 96-97 20.057038980655264 32.46034961800291 27.256177862802033 20.226433538539794 98-99 20.422802870921103 31.326403395830166 27.167680245782112 21.08311348746662 100-101 20.486168903169055 31.442033924177686 26.752303131412596 21.31949404124066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6249.0 1 3980.5 2 1318.5 3 894.5 4 832.0 5 869.0 6 1114.0 7 1464.0 8 1890.0 9 2381.5 10 2787.0 11 2962.5 12 2955.5 13 2747.5 14 2460.5 15 2319.0 16 2287.0 17 2426.0 18 2647.5 19 2986.0 20 3467.0 21 4258.0 22 5492.5 23 7205.0 24 9450.5 25 12227.0 26 15973.0 27 20902.5 28 26736.5 29 32920.5 30 39875.0 31 47946.5 32 59265.0 33 82648.5 34 122195.5 35 196213.0 36 295273.0 37 396513.5 38 512127.5 39 574301.0 40 569661.0 41 518846.0 42 464873.0 43 467665.0 44 457478.5 45 431922.0 46 434252.5 47 394200.0 48 312619.0 49 264645.0 50 227449.0 51 194644.0 52 174605.0 53 152214.5 54 121907.0 55 91074.5 56 70446.5 57 56591.0 58 45978.0 59 36606.5 60 28477.0 61 21991.5 62 16592.0 63 12445.0 64 9574.5 65 7271.0 66 5308.0 67 3905.0 68 2937.5 69 2114.0 70 1512.5 71 1096.0 72 807.5 73 615.5 74 472.5 75 369.5 76 284.0 77 218.5 78 172.0 79 148.0 80 124.0 81 104.5 82 79.5 83 59.5 84 58.0 85 51.0 86 44.0 87 35.0 88 25.5 89 20.5 90 20.0 91 18.5 92 18.0 93 18.5 94 12.0 95 6.0 96 2.5 97 2.0 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22582827733842076 2 0.24178809065173962 3 0.22206940238817963 4 0.21463792174885046 5 0.2238440843319 6 0.2462740922316995 7 0.22638286544583336 8 0.2125304869406825 9 0.2051852755625064 10-11 0.19058728415738962 12-13 0.2176203733487139 14-15 0.21431749306456763 16-17 0.2170596231512189 18-19 0.20575218785008373 20-21 0.19824060003968388 22-23 0.16575406112546878 24-25 0.4942797317765429 26-27 0.3497232605344011 28-29 0.4820972796837122 30-31 0.3164110015491494 32-33 0.36642252465760344 34-35 0.36786445373687626 36-37 0.37532674482661726 38-39 0.3685546078261009 40-41 0.3739895712787446 42-43 0.4274333785630745 44-45 0.382308392889934 46-47 0.3999504567957378 48-49 0.3987858217701713 50-51 0.41178166975387376 52-53 0.4159225942892214 54-55 0.3818770465841686 56-57 0.3949838121893537 58-59 0.3921615749316316 60-61 0.3513315660458977 62-63 0.313921517155875 64-65 0.3403753698794572 66-67 0.3517752365318278 68-69 0.3426060464892724 70-71 0.3138414099848043 72-73 0.3023737603415277 74-75 0.31461783333518195 76-77 0.3213098631646276 78-79 0.33123699028731357 80-81 0.3384466356836777 82-83 0.3567049085977178 84-85 0.38506900924683235 86-87 0.38638153443437556 88-89 0.38044127959497814 90-91 0.3540613719523843 92-93 0.332722053997163 94-95 0.31016880429571625 96-97 0.32662774690570645 98-99 0.3564152903638468 100-101 0.3720731612631299 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8114130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.37588106703073 #Duplication Level Percentage of deduplicated Percentage of total 1 91.04260034623026 39.490530046512944 2 4.156330127207617 3.605689625461486 3 1.2594886157223921 1.6389428520256093 4 0.7978707407237761 1.3843338542599288 5 0.4863721695653172 1.0548410690689451 6 0.3154258625790443 0.8209124820416522 7 0.2283638677207125 0.6933838776382534 8 0.1907075885899837 0.6617687745007488 9 0.15867404954828543 0.6194364031467513 >10 1.0498155610700795 9.185958327395728 >50 0.13218166125970107 4.023229736962608 >100 0.1398623097364993 13.37721761589872 >500 0.023222625015424277 6.940539681671666 >1k 0.01848091110301571 14.306023015185476 >5k 4.3126414563856674E-4 1.2077556311693407 >10k+ 1.7229978198439038E-4 0.9894370070603455 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 16469 0.2029669231328559 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 15581 0.1920230511465801 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12661 0.15603644506558312 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 12597 0.15524769753504072 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11488 0.14158018173236073 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10638 0.13110462859234445 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 9730 0.11991427300277416 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9294 0.11454093045095409 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 8664 0.10677669694717734 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0020951106280032487 0.0 0.0 2 0.0023169458709682987 0.0 0.0 3 0.002563429474262798 0.0 0.0 4 0.002809913077557298 0.0 0.0 5 0.003167314302334323 0.0 2.4648360329449985E-5 6 0.003475418806452448 0.0 2.4648360329449985E-5 7 0.003943737652711997 0.0 2.4648360329449985E-5 8 0.004424380679136272 0.0 2.4648360329449985E-5 9 0.004978968786548897 0.0 2.4648360329449985E-5 10-11 0.008275686980612831 0.0 3.081045041181248E-5 12-13 0.011276624850723367 0.0 4.929672065889997E-5 14-15 0.014234428090257366 0.0 4.929672065889997E-5 16-17 0.016791695474437804 0.0 4.929672065889997E-5 18-19 0.019792633344548338 0.0 6.778299090598745E-5 20-21 0.02347756321380111 0.0 7.394508098834995E-5 22-23 0.02752605639791327 0.0 7.394508098834995E-5 24-25 0.03221540695059113 0.0 7.394508098834995E-5 26-27 0.037225186187551836 0.0 7.394508098834995E-5 28-29 0.04307300967571385 0.0 7.394508098834995E-5 30-31 0.06756731775310476 0.0 7.394508098834995E-5 32-33 0.07454280372633912 0.0 7.394508098834995E-5 34-35 0.08116088847479644 0.0 7.394508098834995E-5 36-37 0.0880501051868777 0.0 7.394508098834995E-5 38-39 0.09518580550225347 0.0 7.394508098834995E-5 40-41 0.10299317363660676 0.0 8.010717107071244E-5 42-43 0.11139210241886684 0.0 8.626926115307494E-5 44-45 0.12059210291183405 0.0 8.626926115307494E-5 46-47 0.1298783726659543 0.0 9.859344131779994E-5 48-49 0.14005814548201717 0.0 9.859344131779994E-5 50-51 0.15044742936088035 0.0 1.2324180164724992E-4 52-53 0.16146524642814447 0.0 1.355659818119749E-4 54-55 0.1737216436019635 0.0 1.355659818119749E-4 56-57 0.18705640654019592 0.0 1.478901619766999E-4 58-59 0.20126002418004146 0.0 1.478901619766999E-4 60-61 0.21734924138508996 0.0 1.478901619766999E-4 62-63 0.23523778889418828 0.0 1.5405225205906238E-4 64-65 0.2533111991057575 0.0 1.7253852230614988E-4 66-67 0.2731223187205529 0.0 1.7253852230614988E-4 68-69 0.29540443645837566 0.0 1.7253852230614988E-4 70-71 0.31927637343744797 0.0 1.7253852230614988E-4 72-73 0.34600135812465416 0.0 1.848627024708749E-4 74-75 0.37555474215966467 0.0 1.848627024708749E-4 76-77 0.40775166284000874 0.0 1.848627024708749E-4 78-79 0.44379372773174697 0.0 1.848627024708749E-4 80-81 0.48443271182492764 0.0 1.848627024708749E-4 82-83 0.5296501288493036 0.0 1.848627024708749E-4 84-85 0.5816951416849372 0.0 1.848627024708749E-4 86-87 0.6383986946228369 0.0 1.9718688263559988E-4 88-89 0.702774049713278 0.0 1.9718688263559988E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 5830 0.0 84.7548 9 CGGGAGA 7825 0.0 81.832756 4 AGAGCGG 6380 0.0 81.01753 8 AAGCGGC 1940 0.0 78.21921 9 CGGAAAG 3245 0.0 76.88628 5 GAGAGCG 7090 0.0 76.319244 7 TCGGGAG 8310 0.0 72.43332 3 ATTCGGA 940 0.0 72.16092 2 TCGGGAA 2660 0.0 68.474266 3 GTTCGGA 1005 0.0 67.49379 2 ACCGGAA 1115 0.0 66.788635 3 TCGGAGA 1985 0.0 66.19071 4 GAACCGG 1105 0.0 66.112236 1 AACCGGA 1050 0.0 64.149445 2 CGGAGAG 3860 0.0 63.407436 5 GATCCGG 1550 0.0 62.128048 1 GGATCGG 2955 0.0 61.644897 1 ATCGGGA 11625 0.0 60.797707 2 AAGCGTC 940 0.0 60.032196 9 GATCGGG 12220 0.0 59.976437 1 >>END_MODULE