FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8114130
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC164690.2029669231328559No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG155810.1920230511465801No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA126610.15603644506558312No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA125970.15524769753504072No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC114880.14158018173236073No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG106380.13110462859234445No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC97300.11991427300277416No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA92940.11454093045095409No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT86640.10677669694717734Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC58300.084.75489
CGGGAGA78250.081.8327564
AGAGCGG63800.081.017538
AAGCGGC19400.078.219219
CGGAAAG32450.076.886285
GAGAGCG70900.076.3192447
TCGGGAG83100.072.433323
ATTCGGA9400.072.160922
TCGGGAA26600.068.4742663
GTTCGGA10050.067.493792
ACCGGAA11150.066.7886353
TCGGAGA19850.066.190714
GAACCGG11050.066.1122361
AACCGGA10500.064.1494452
CGGAGAG38600.063.4074365
GATCCGG15500.062.1280481
GGATCGG29550.061.6448971
ATCGGGA116250.060.7977072
AAGCGTC9400.060.0321969
GATCGGG122200.059.9764371