##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34298-1.342000000357ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5187311 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63413433279786 33.0 33.0 33.0 27.0 33.0 2 31.504182031885115 33.0 33.0 33.0 27.0 33.0 3 31.45877989578801 33.0 33.0 33.0 27.0 33.0 4 35.09667879947819 37.0 37.0 37.0 33.0 37.0 5 35.10331152306079 37.0 37.0 37.0 33.0 37.0 6 35.20190210303566 37.0 37.0 37.0 33.0 37.0 7 35.05457162680241 37.0 37.0 37.0 33.0 37.0 8 35.02958584900732 37.0 37.0 37.0 33.0 37.0 9 34.84867728192892 37.0 37.0 37.0 33.0 37.0 10-11 34.79349474130238 37.0 37.0 37.0 33.0 37.0 12-13 34.80535473967148 37.0 37.0 37.0 33.0 37.0 14-15 36.3766244206295 40.0 37.0 40.0 33.0 40.0 16-17 36.29188793577251 40.0 37.0 40.0 33.0 40.0 18-19 36.29471840034269 40.0 37.0 40.0 33.0 40.0 20-21 36.25242953430014 40.0 37.0 40.0 33.0 40.0 22-23 36.417383977941554 40.0 37.0 40.0 33.0 40.0 24-25 36.08506681014499 40.0 37.0 40.0 27.0 40.0 26-27 35.94575330069857 37.0 37.0 40.0 27.0 40.0 28-29 35.79150160844414 37.0 37.0 40.0 27.0 40.0 30-31 35.83813521109492 37.0 37.0 40.0 27.0 40.0 32-33 35.707163692325366 37.0 37.0 40.0 27.0 40.0 34-35 35.62258171912191 37.0 37.0 40.0 27.0 40.0 36-37 35.528611162893455 37.0 37.0 40.0 27.0 40.0 38-39 35.51260855576232 37.0 37.0 40.0 27.0 40.0 40-41 35.30919218068861 37.0 37.0 40.0 27.0 40.0 42-43 35.17717262373511 37.0 37.0 40.0 27.0 40.0 44-45 35.087221394668646 37.0 37.0 40.0 27.0 40.0 46-47 34.97407741698926 37.0 37.0 40.0 27.0 40.0 48-49 34.8262867794123 37.0 35.0 40.0 22.0 40.0 50-51 33.692095673461644 37.0 33.0 38.5 22.0 38.5 52-53 34.2020264449153 37.0 33.0 38.5 24.5 40.0 54-55 34.971727644631294 37.0 37.0 40.0 27.0 40.0 56-57 35.04669847248411 37.0 37.0 40.0 27.0 40.0 58-59 34.74856818879762 37.0 33.0 40.0 27.0 40.0 60-61 34.49831964962193 37.0 33.0 40.0 27.0 40.0 62-63 34.224735898811545 37.0 33.0 40.0 24.5 40.0 64-65 33.95968113344274 37.0 33.0 37.0 22.0 40.0 66-67 33.72433810118576 37.0 33.0 37.0 22.0 40.0 68-69 33.55456892790889 37.0 33.0 37.0 22.0 40.0 70-71 33.22226785708433 37.0 33.0 37.0 22.0 40.0 72-73 33.07457910273743 37.0 33.0 37.0 22.0 37.0 74-75 32.8661443279572 37.0 33.0 37.0 22.0 37.0 76-77 32.59849158841642 37.0 33.0 37.0 22.0 37.0 78-79 32.29699886897084 37.0 33.0 37.0 22.0 37.0 80-81 32.02597125948299 35.0 33.0 37.0 22.0 37.0 82-83 31.81099321016226 33.0 33.0 37.0 22.0 37.0 84-85 31.634934940280232 33.0 33.0 37.0 18.5 37.0 86-87 31.439594907650612 33.0 33.0 37.0 15.0 37.0 88-89 31.330147835747653 33.0 33.0 37.0 15.0 37.0 90-91 31.196341707678602 33.0 33.0 37.0 15.0 37.0 92-93 30.983936571375807 33.0 33.0 37.0 15.0 37.0 94-95 30.8468116717891 33.0 30.0 37.0 15.0 37.0 96-97 30.666206344674535 33.0 27.0 37.0 15.0 37.0 98-99 30.528380021942006 33.0 27.0 37.0 15.0 37.0 100-101 29.234316488832075 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.052939127125561924 2306 2 0.06078002337169508 2306 3 0.07480214251842199 2306 4 0.07740287211069585 2306 5 0.12851300979352942 2306 6 0.10273241185611681 2306 7 0.13342837230096194 2306 8 0.0798029664062554 2306 9 0.1849881291709039 2306 10-11 0.1986900452403617 2306 12-13 0.11303899131092265 2306 14-15 0.35977044914399414 2306 16-17 0.34698000463291834 2306 18-19 0.35098768650567536 2306 20-21 0.27326503594065343 2306 22-23 0.39094199181571554 2306 24-25 0.3377935789283697 2306 26-27 0.22558223076794093 2306 28-29 0.3280948115305975 2306 30-31 0.3543150650575555 2306 32-33 0.42752875827954995 2306 34-35 0.399642332624218 2306 36-37 0.36530438283840994 2306 38-39 0.336426211961772 2306 40-41 0.4533420480953865 2306 42-43 0.33879353194333106 2306 44-45 0.3552162254141322 2306 46-47 0.31437323174526455 2306 48-49 0.4748524548654487 2306 50-51 0.4050642129857991 2306 52-53 0.3989872647305859 2306 54-55 0.5290738818949521 2306 56-57 0.5325570713730912 2306 58-59 0.6367131376050068 2306 60-61 0.49775933826452246 2306 62-63 0.5678698955517874 2306 64-65 0.4999505399765667 2306 66-67 0.4856087188623732 2306 68-69 0.41666828930692645 2306 70-71 0.44774767496426904 2306 72-73 0.3908460383863428 2306 74-75 0.5203170965868935 2306 76-77 0.4912429369751692 2306 78-79 0.520112922186506 2306 80-81 0.41826807124190424 2306 82-83 0.3732429416098135 2306 84-85 0.45224778915708086 2306 86-87 0.49321419531068145 2306 88-89 0.39656036273891715 2306 90-91 0.4018221089651206 2306 92-93 0.377950696025831 2306 94-95 0.3540163921432189 2306 96-97 0.422613372990984 2306 98-99 0.4161387533872727 2306 100-101 0.21245421664848863 2307 1 0.11115053509842099 2307 2 0.033878297475631314 2307 3 -0.004519268694661349 2307 4 0.16303402496352248 2307 5 0.0657459295217322 2307 6 0.1133319367820178 2307 7 0.11826072641937913 2307 8 0.08230740980238949 2307 9 0.17060188964206446 2307 10-11 0.13779866185508638 2307 12-13 0.09420977258623964 2307 14-15 0.34209809183299456 2307 16-17 0.293205351527547 2307 18-19 0.3370076663019219 2307 20-21 0.303433283408026 2307 22-23 0.2340750685518529 2307 24-25 0.27516918170542937 2307 26-27 0.21641266338556875 2307 28-29 0.195780689631448 2307 30-31 0.31480934224922663 2307 32-33 0.36631636037044757 2307 34-35 0.34550556655889864 2307 36-37 0.3579366840649314 2307 38-39 0.20402411558415423 2307 40-41 0.3617926608790256 2307 42-43 0.31777570236960884 2307 44-45 0.3023997614997498 2307 46-47 0.3638628368190737 2307 48-49 0.39525053193514026 2307 50-51 0.18369400899459976 2307 52-53 0.26169233997050156 2307 54-55 0.2166078029954832 2307 56-57 0.35916178661453557 2307 58-59 0.4711554735209944 2307 60-61 0.5454108306738235 2307 62-63 0.5690225875589192 2307 64-65 0.4729266802496994 2307 66-67 0.32887304256080796 2307 68-69 0.22883971028833372 2307 70-71 0.29788580144738575 2307 72-73 0.21628945477425532 2307 74-75 0.23635156209279273 2307 76-77 0.25169516272351444 2307 78-79 0.3113271188967275 2307 80-81 0.3001423803116694 2307 82-83 0.21096073353974631 2307 84-85 0.2843581759827103 2307 86-87 0.21911091553635131 2307 88-89 0.2104311147092588 2307 90-91 0.21720108419646067 2307 92-93 0.18115598688362766 2307 94-95 0.13968813714707906 2307 96-97 0.2220958305106855 2307 98-99 0.2362950644392967 2307 100-101 -0.028133318255402884 2201 1 0.1612693060439767 2201 2 0.08925323267768803 2201 3 0.06791681322727428 2201 4 0.16073738214712563 2201 5 0.0970473452809415 2201 6 0.11541386868826464 2201 7 0.22390515821727064 2201 8 0.08558712843628768 2201 9 0.21693859535798055 2201 10-11 0.13934043030581478 2201 12-13 0.05233843616225187 2201 14-15 0.24628639573924715 2201 16-17 0.41689831308394787 2201 18-19 0.40112913188836075 2201 20-21 0.38080557619764477 2201 22-23 0.2690145691612571 2201 24-25 0.23431946937361658 2201 26-27 0.16864468704917357 2201 28-29 0.27346992888193 2201 30-31 0.42764379932481944 2201 32-33 0.4204258665104348 2201 34-35 0.4652112061904461 2201 36-37 0.4907494955934979 2201 38-39 0.5537643224261259 2201 40-41 0.4547825311617757 2201 42-43 0.4082163207977132 2201 44-45 0.460303382252782 2201 46-47 0.398525564608768 2201 48-49 0.4678833102889044 2201 50-51 0.36025326097061594 2201 52-53 0.46011409829628036 2201 54-55 0.5898760021154388 2201 56-57 0.6543208330575396 2201 58-59 0.6236106762540317 2201 60-61 0.6057138228217838 2201 62-63 0.6341575504052059 2201 64-65 0.6710309774152279 2201 66-67 0.6272618817977573 2201 68-69 0.5091208022485318 2201 70-71 0.6434152526543144 2201 72-73 0.5347774402820562 2201 74-75 0.4929012471617824 2201 76-77 0.5239948655631892 2201 78-79 0.5127812963485994 2201 80-81 0.5373321357785343 2201 82-83 0.47981620356972243 2201 84-85 0.47330876137469957 2201 86-87 0.49434760140112743 2201 88-89 0.5171550769886224 2201 90-91 0.414794339055657 2201 92-93 0.23076745386572028 2201 94-95 0.2977595498982737 2201 96-97 0.5503153455706808 2201 98-99 0.5083831174756561 2201 100-101 0.2258103354903973 1101 1 0.1719336498172659 1101 2 0.06729372323630045 1101 3 0.06965958304171238 1101 4 0.19634865511081756 1101 5 0.07754909584793523 1101 6 0.1528337376636486 1101 7 0.24990315734078905 1101 8 0.18383715122804745 1101 9 0.16774433400289013 1101 10-11 0.17194401584928443 1101 12-13 0.19197731774267623 1101 14-15 0.23150766472838313 1101 16-17 0.23004178040615386 1101 18-19 0.2902015433043985 1101 20-21 0.27341130996431673 1101 22-23 0.06968162295460445 1101 24-25 0.025629481673178134 1101 26-27 0.1902738409471212 1101 28-29 0.0516555619570056 1101 30-31 0.20435918903989858 1101 32-33 0.33034156599349984 1101 34-35 0.2838707289070328 1101 36-37 0.21682954504761653 1101 38-39 0.10229782271241561 1101 40-41 0.2060155602984466 1101 42-43 0.13702480864851196 1101 44-45 0.013286023729619956 1101 46-47 0.14527578972737132 1101 48-49 0.1260489675872165 1101 50-51 0.24300550775817698 1101 52-53 0.31467471417757764 1101 54-55 0.3849079185744486 1101 56-57 0.37801224624253393 1101 58-59 0.4815004633070359 1101 60-61 0.34279736841170205 1101 62-63 0.34022998461299636 1101 64-65 0.39427250327948116 1101 66-67 0.4455784035434931 1101 68-69 0.3870753838718173 1101 70-71 0.3389121359813032 1101 72-73 0.2930335548710161 1101 74-75 0.30199050548597484 1101 76-77 0.23862113170535082 1101 78-79 0.3032105521297197 1101 80-81 0.29114553185451797 1101 82-83 0.1809782383779286 1101 84-85 0.2053369349871197 1101 86-87 0.07482787473428942 1101 88-89 0.1876496261596401 1101 90-91 0.1601700861854951 1101 92-93 0.14113928841034706 1101 94-95 0.20181519337758758 1101 96-97 0.29764046280828893 1101 98-99 0.09194482656165093 1101 100-101 -0.06878418912949869 2304 1 0.12528761197404492 2304 2 0.11075874315378442 2304 3 0.21169021803009258 2304 4 0.3204735602656541 2304 5 0.24210397563168584 2304 6 0.14799070641216616 2304 7 0.30428204006474147 2304 8 0.19031255669112568 2304 9 0.33331361246829516 2304 10-11 0.29423018446027527 2304 12-13 0.1571732174676228 2304 14-15 0.2038280665162162 2304 16-17 0.3209923660750533 2304 18-19 0.26213923003081163 2304 20-21 0.30260880130969525 2304 22-23 0.12947668329984197 2304 24-25 0.15742647152474376 2304 26-27 0.16875695554379178 2304 28-29 0.1790063141977214 2304 30-31 0.175403997452662 2304 32-33 0.3153193514774486 2304 34-35 0.25008732562539393 2304 36-37 0.2285014281983777 2304 38-39 0.050499765852748624 2304 40-41 0.10942724463001952 2304 42-43 0.22287190935036705 2304 44-45 0.3725506180294289 2304 46-47 0.2135042895139705 2304 48-49 0.1621590413293319 2304 50-51 0.08913478764625182 2304 52-53 0.26282886424633745 2304 54-55 0.3069807596613785 2304 56-57 0.37181174356315694 2304 58-59 0.3906847356075076 2304 60-61 0.368775681471881 2304 62-63 0.3966102485196714 2304 64-65 0.29432570548937065 2304 66-67 0.36620967221613654 2304 68-69 0.3630062002515757 2304 70-71 0.3850005879896514 2304 72-73 0.37686186141058897 2304 74-75 0.33405891552533973 2304 76-77 0.26237206533744484 2304 78-79 0.2973534926855095 2304 80-81 0.2684919864236761 2304 82-83 0.23994933134990148 2304 84-85 0.36459197067260973 2304 86-87 0.25832622383563475 2304 88-89 0.2561471009071141 2304 90-91 0.21956650044546677 2304 92-93 0.19772072780428473 2304 94-95 0.2796023074763241 2304 96-97 0.36607940030532404 2304 98-99 0.27911472092784706 2304 100-101 0.10077303214427147 2202 1 -0.040471068420952605 2202 2 0.0257475169548691 2202 3 -0.06837951113678997 2202 4 0.0212578956506988 2202 5 -0.0546062599900452 2202 6 -0.045481100500637694 2202 7 0.05627252819459727 2202 8 -0.06665282448584975 2202 9 0.020032573869350756 2202 10-11 0.004702875083019364 2202 12-13 -0.07267939478580132 2202 14-15 0.12414166366094292 2202 16-17 0.02498333973159106 2202 18-19 0.03933084352075156 2202 20-21 0.13630398902955676 2202 22-23 0.00896943208303469 2202 24-25 -0.20281118393799602 2202 26-27 -0.03935249604226243 2202 28-29 -0.04676532642005071 2202 30-31 -0.007409407022741732 2202 32-33 0.029206848865705126 2202 34-35 0.0735570602726412 2202 36-37 0.15450506408247833 2202 38-39 0.03545959215921357 2202 40-41 0.040534176957706336 2202 42-43 -0.06941568771215856 2202 44-45 0.03262302744060008 2202 46-47 -0.08846922230822685 2202 48-49 3.239217373476322E-4 2202 50-51 0.030507612195584954 2202 52-53 0.03117706308793089 2202 54-55 0.08081922056748425 2202 56-57 0.21351195609882012 2202 58-59 0.17466023577705414 2202 60-61 0.191149213331677 2202 62-63 0.2017062877522804 2202 64-65 0.24781454008832782 2202 66-67 0.21212122929249944 2202 68-69 0.2867185697930452 2202 70-71 0.24075654104341737 2202 72-73 0.3284032373606678 2202 74-75 0.3296685811884146 2202 76-77 0.18903460888071066 2202 78-79 0.2098459999898168 2202 80-81 0.11877189212703598 2202 82-83 0.17140889134456572 2202 84-85 0.17683544217981506 2202 86-87 0.19335643866620345 2202 88-89 0.08781493643430949 2202 90-91 0.036412263946875356 2202 92-93 0.08704626959040596 2202 94-95 0.1447978796785101 2202 96-97 0.14832449451634133 2202 98-99 0.06008496507840633 2202 100-101 -0.10171563461830502 1102 1 -0.13870157900112545 1102 2 -0.21938262378664675 1102 3 -0.2273929815671245 1102 4 -0.1696929018634279 1102 5 -0.20639385312314573 1102 6 -0.23496462572541077 1102 7 -0.034123432102411755 1102 8 -0.16909341895316743 1102 9 -0.08262930581107497 1102 10-11 -0.07941377066732258 1102 12-13 -0.1798309755529246 1102 14-15 -0.24695114302467402 1102 16-17 -0.18151321937445175 1102 18-19 -0.1406414978083248 1102 20-21 -0.15818586655272782 1102 22-23 -0.2432616029898469 1102 24-25 -0.26344501593553105 1102 26-27 -0.12452044462612832 1102 28-29 -0.16723943771390992 1102 30-31 -0.04139502863374389 1102 32-33 -0.03563863178194282 1102 34-35 0.1460661953192144 1102 36-37 0.042504976138836525 1102 38-39 0.030109925077390187 1102 40-41 -0.00937270974236526 1102 42-43 -0.01172188686726372 1102 44-45 -0.012080348961355014 1102 46-47 -0.03554874078906067 1102 48-49 -0.07815787392916462 1102 50-51 0.2081884472957114 1102 52-53 0.07724443742359455 1102 54-55 0.22999890843887272 1102 56-57 0.3543227964597264 1102 58-59 0.35437243031155674 1102 60-61 0.3860158400671807 1102 62-63 0.41734032970536106 1102 64-65 0.33848910742146643 1102 66-67 0.30218744055371616 1102 68-69 0.2654162037129808 1102 70-71 0.20520221485015355 1102 72-73 0.20956584832018876 1102 74-75 0.29558618195914477 1102 76-77 0.12629163486449357 1102 78-79 0.23814477781090204 1102 80-81 0.20651611399220116 1102 82-83 0.17340774728495134 1102 84-85 0.24051632323247674 1102 86-87 0.20679848539200307 1102 88-89 0.12281317756151111 1102 90-91 0.06704636135990683 1102 92-93 0.04989610305209169 1102 94-95 0.14627723431480533 1102 96-97 0.26664069864699513 1102 98-99 0.02278683930790848 1102 100-101 0.036262073649666604 2305 1 -0.07095494004413894 2305 2 -0.09595277472617525 2305 3 -0.10325657569499569 2305 4 -0.12049351810155429 2305 5 -0.053884730143970216 2305 6 -0.05456193463914616 2305 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522.0 4 514.0 5 480.0 6 994.0 7 4324.0 8 20627.0 9 45055.0 10 49913.0 11 33350.0 12 21796.0 13 16762.0 14 15196.0 15 16023.0 16 16682.0 17 17450.0 18 18347.0 19 19893.0 20 22319.0 21 24570.0 22 26971.0 23 29751.0 24 33315.0 25 38583.0 26 44595.0 27 53042.0 28 64229.0 29 77879.0 30 96668.0 31 125890.0 32 170234.0 33 240759.0 34 359667.0 35 590343.0 36 1071567.0 37 1493076.0 38 320531.0 39 60.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.836346546389315 24.063997650611547 26.458074718667497 13.641581084331648 2 31.7809001129088 21.97646248589123 34.39925193812605 11.843385463073913 3 38.647120113772324 18.290802984804145 27.533143184701707 15.528933716721816 4 29.841418809703153 17.022107709715304 36.72578244971977 16.410691030861773 5 30.73441546711335 16.106578874917044 37.29887739005021 15.860128267919398 6 24.780926784152445 16.887160239887734 43.162167708841814 15.169745267118012 7 34.38511939631787 17.147560988804614 31.935578232449153 16.53174138242836 8 29.70378549968472 28.135103578096217 26.047970938786612 16.11313998343245 9 30.716271935882368 26.99508473970563 24.43476438334201 17.853878941069997 10-11 25.568398502471336 27.295885330722275 28.864539556869882 18.271176609936507 12-13 26.893563140602318 24.10946817552751 28.469208054775237 20.527760629094935 14-15 25.04122387604989 25.043947824979906 29.77928189558254 20.135546403387668 16-17 23.811283712550114 26.710899662703042 31.06180634993067 18.416010274816173 18-19 22.910739717109745 27.203265373929646 31.008447880109934 18.87754702885067 20-21 22.825009049464352 28.008024566639794 30.12845796741111 19.038508416484746 22-23 23.6603132254041 27.419383024369857 30.121479043047568 18.798824707178476 24-25 23.148805508953707 27.852171216728195 30.256255117687065 18.742768156631033 26-27 22.078848761537092 28.674640549921115 30.19167865825036 19.054832030291433 28-29 21.89532028805648 28.41504331976077 29.784484541289785 19.90515185089297 30-31 22.32966234344886 28.027396666869077 29.49262752963937 20.150313460042693 32-33 21.314313614843254 28.016803410678698 30.697433642093696 19.971449332384353 34-35 20.698541579415963 28.204641510021727 31.98744499275535 19.10937191780696 36-37 20.637449836867628 29.101621571206348 30.896493713390516 19.364434878535512 38-39 20.8358752918536 28.885945924923657 29.34382738945552 20.934351393767226 40-41 21.3936902713568 28.014460649432777 29.840124412823947 20.75172466638648 42-43 21.735316475828355 28.284619687791647 31.043196182788535 18.936867653591467 44-45 21.007574505602022 29.184255431633808 31.240365446383407 18.567804616380766 46-47 21.812821025974724 28.9528412867323 30.58419227358294 18.650145413710035 48-49 21.48097474890044 29.57311163521267 29.828834529722936 19.117079086163955 50-51 20.482495606588845 29.593922695514664 29.93427474332287 19.989306954573625 52-53 21.628915418226157 29.036996413825783 29.46687079077508 19.867217377172977 54-55 22.735895125877917 28.82621829830347 29.030618183626665 19.407268392191952 56-57 22.985036410317747 28.540072638709706 28.358359741603444 20.1165312093691 58-59 22.20574704946784 28.30974210736759 29.205062323956067 20.279448519208508 60-61 20.717812377640453 28.370826620485367 29.47651199645902 21.43484900541516 62-63 21.736436561011 28.34453666865623 29.018738275705797 20.900288494626974 64-65 21.136549595049683 28.652183752855542 29.486244068885075 20.7250225832097 66-67 21.23800981413767 29.002894175289658 28.761240549871058 20.997855460701615 68-69 21.606050602943107 28.564228512435342 29.883198904828333 19.946521979793218 70-71 20.93436525790347 30.450734735734347 29.30164475064584 19.313255255716346 72-73 20.392151706551758 31.744822956300872 28.356092126222066 19.506933210925304 74-75 21.586963693625297 31.193051923766397 27.63948616538114 19.580498217227166 76-77 22.07623854295969 29.95199857042941 28.262196628421982 19.709566258188918 78-79 21.905356518402296 29.85922853632647 29.023104221403173 19.212310723868057 80-81 20.691452069850257 30.730949496313276 28.82639962760868 19.751198806227787 82-83 19.787143813689944 30.35909650990764 29.07679000264854 20.776969673753875 84-85 19.591257196705914 29.443326373328542 29.90359817636844 21.061818253597107 86-87 19.8649564361086 29.42254802342928 30.611787152486247 20.100708387975878 88-89 20.068724219830422 30.060721488200397 29.9852223769574 19.885331915011783 90-91 20.393576972997046 29.71379333093056 29.425528247385824 20.46710144868657 92-93 19.73368850043669 30.62365616770969 29.21719753310761 20.425457798746013 94-95 19.53394541892992 31.05215425639915 29.411125442095837 20.002774882575093 96-97 20.373214144114 31.368845119127975 28.53322412382579 19.724716612932237 98-99 20.757024134970028 30.4941050923594 28.188290356352656 20.560580416317904 100-101 20.52385844595343 30.380032180257427 28.189942271547697 20.906167102241444 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6462.0 1 4081.5 2 1387.0 3 1247.5 4 1266.0 5 1127.0 6 1408.0 7 1865.0 8 2254.5 9 2496.5 10 2607.5 11 2653.5 12 2515.5 13 2261.0 14 2001.5 15 1801.0 16 1778.0 17 1849.5 18 1909.5 19 2045.5 20 2383.0 21 2758.5 22 3226.0 23 3953.0 24 5010.5 25 6406.0 26 8034.5 27 10232.0 28 12763.0 29 15535.0 30 19042.5 31 24043.5 32 35235.5 33 55486.5 34 84124.5 35 124095.5 36 174362.5 37 257552.5 38 363034.5 39 423160.0 40 413715.5 41 355547.0 42 309594.5 43 303073.0 44 299246.0 45 291646.5 46 290693.0 47 262219.0 48 207232.5 49 166134.5 50 137890.0 51 116110.0 52 95646.0 53 70783.5 54 49274.0 55 36398.0 56 27243.5 57 20367.5 58 15450.0 59 11553.5 60 8690.5 61 6746.5 62 5094.5 63 3661.0 64 2795.0 65 2106.0 66 1488.5 67 1147.0 68 903.0 69 640.0 70 442.5 71 346.0 72 272.5 73 208.0 74 152.5 75 109.0 76 88.5 77 72.5 78 57.5 79 47.5 80 35.5 81 28.0 82 23.5 83 21.0 84 20.0 85 12.5 86 9.0 87 6.5 88 5.5 89 8.5 90 5.5 91 3.5 92 2.5 93 2.5 94 3.5 95 3.5 96 3.5 97 2.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2216177129152272 2 0.23784962960578226 3 0.218995930646919 4 0.21151614005792213 5 0.22026826615948034 6 0.2420714701701903 7 0.2237575499136258 8 0.21064863857208482 9 0.20388212698255417 10-11 0.18983824181738862 12-13 0.21545845236578257 14-15 0.2121041132872118 16-17 0.21514036848764226 18-19 0.2037375434015813 20-21 0.19680717042028134 22-23 0.1641794756474019 24-25 0.49500598672414287 26-27 0.3481669018880881 28-29 0.4830922996519777 30-31 0.31455603876459304 32-33 0.3654205425508515 34-35 0.36655793338783815 36-37 0.3744136412873645 38-39 0.3672808512927025 40-41 0.3731220279640068 42-43 0.4255480344247723 44-45 0.38002348422911214 46-47 0.39853018259364054 48-49 0.396843374148957 50-51 0.41132101005704114 52-53 0.41446329321685166 54-55 0.3806307352691983 56-57 0.3926697281115399 58-59 0.39089616951827255 60-61 0.3492946538196765 62-63 0.3114812279425698 64-65 0.33815207917936674 66-67 0.34961273769781687 68-69 0.3414196681093538 70-71 0.31098964376726207 72-73 0.29951934634341376 74-75 0.31139447779398616 76-77 0.3186140179372318 78-79 0.3293999530778085 80-81 0.3369857716261855 82-83 0.35513583049098074 84-85 0.38419713026652924 86-87 0.38585502199501825 88-89 0.3797825115941573 90-91 0.3524754926010798 92-93 0.3308554277929355 94-95 0.3069220256892251 96-97 0.3242720554059704 98-99 0.35472135755885853 100-101 0.3708183295738389 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5187311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.02199618938441 #Duplication Level Percentage of deduplicated Percentage of total 1 86.55033822313824 32.04266291886955 2 5.972096713353242 4.421978835287978 3 2.187529890466266 2.429601698070198 4 1.0567046601697896 1.5648526360844282 5 0.7775443337810697 1.4393121681160101 6 0.48164894421504517 1.0698963226410256 7 0.3232165922822306 0.8376286413472986 8 0.290244239186964 0.8596336893736446 9 0.19766128379337267 0.6586033765848367 >10 1.6674898441872272 12.369386199479457 >50 0.22560192449471167 5.846636520856309 >100 0.21984454156485475 17.33186477253954 >500 0.03545203805007709 8.919142660341192 >1k 0.013989999239329668 8.353250936993549 >5k 5.306446501735832E-4 1.3235357146528668 >10k+ 1.0612742742080201E-4 0.5320129087622311 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 13955 0.2690218496635347 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 13126 0.25304054451333263 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 9415 0.18150058864795265 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 9034 0.17415574273453047 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6993 0.13480973089911133 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6970 0.1343663412507945 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6566 0.12657810568905548 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5956 0.1148186411032614 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 5789 0.11159924670026533 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG 5686 0.10961363218823779 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5504 0.10610507062329597 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 5458 0.10521829132666231 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001484391431321546 0.0 0.0 2 0.001619336106896232 0.0 0.0 3 0.0019085032688419877 0.0 0.0 4 0.00202417013362029 0.0 0.0 5 0.002216948241584127 0.0 0.0 6 0.002641060079104569 0.0 1.9277810796383715E-5 7 0.0030458941058286266 0.0 3.855562159276743E-5 8 0.0034700059433490685 0.0 3.855562159276743E-5 9 0.003932673402462277 0.0 3.855562159276743E-5 10-11 0.006891817359707177 0.0 3.855562159276743E-5 12-13 0.010091933951906874 0.0 3.855562159276743E-5 14-15 0.014400524664898634 0.0 3.855562159276743E-5 16-17 0.0190561159722253 0.0 3.855562159276743E-5 18-19 0.024482819711407318 0.0 3.855562159276743E-5 20-21 0.02907093868094664 0.0 5.783343238915114E-5 22-23 0.03415064182579375 0.0 5.783343238915114E-5 24-25 0.03960626228117034 0.0 5.783343238915114E-5 26-27 0.046700496654239546 0.0 5.783343238915114E-5 28-29 0.054556204553765905 0.0 5.783343238915114E-5 30-31 0.0827403639380789 0.0 7.711124318553486E-5 32-33 0.09239854714706713 0.0 7.711124318553486E-5 34-35 0.10148803493756206 0.0 7.711124318553486E-5 36-37 0.1115028576462834 0.0 7.711124318553486E-5 38-39 0.12236590403004563 0.0 7.711124318553486E-5 40-41 0.13439525796698906 0.0 7.711124318553486E-5 42-43 0.147002946227824 0.0 7.711124318553486E-5 44-45 0.16021788552874505 0.0 7.711124318553486E-5 46-47 0.17455093785585635 0.0 7.711124318553486E-5 48-49 0.18977076947960128 0.0 7.711124318553486E-5 50-51 0.20548218527865403 0.0 7.711124318553486E-5 52-53 0.22245629768486988 0.0 7.711124318553486E-5 54-55 0.23964246600984596 0.0 7.711124318553486E-5 56-57 0.25797566407720685 0.0 7.711124318553486E-5 58-59 0.2775619198463327 0.0 7.711124318553486E-5 60-61 0.29856509470899273 0.0 7.711124318553486E-5 62-63 0.320030937030766 0.0 9.638905398191857E-5 64-65 0.3439739780398746 0.0 1.1566686477830229E-4 66-67 0.36938213266950837 0.0 1.1566686477830229E-4 68-69 0.39658312370320575 0.0 1.1566686477830229E-4 70-71 0.42489258885769526 0.0 1.1566686477830229E-4 72-73 0.45406955549802197 0.0 1.1566686477830229E-4 74-75 0.48533816460975643 0.0 1.1566686477830229E-4 76-77 0.5187658885306857 0.0 1.1566686477830229E-4 78-79 0.5548153947199232 0.0 1.1566686477830229E-4 80-81 0.5932649883533105 0.0 1.1566686477830229E-4 82-83 0.6343556420658025 0.0 1.1566686477830229E-4 84-85 0.6794271637077476 0.0 1.1566686477830229E-4 86-87 0.7290193319814446 0.0 1.34944675574686E-4 88-89 0.7830839523599029 0.0 1.34944675574686E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGAGA 2060 0.0 77.839676 4 GAGCGGC 1465 0.0 73.49325 9 AGAGCGG 1595 0.0 70.180214 8 GAGAGCG 2010 0.0 69.37821 7 TCGGGAA 745 0.0 63.042072 3 CGGAAAG 1070 0.0 62.072006 5 ATTCGGA 300 0.0 61.67655 2 TCGGGAG 2620 0.0 60.29683 3 ACCGGAA 405 0.0 55.054695 3 GAACCGG 345 0.0 55.01121 1 AAGCGGC 665 0.0 54.91972 9 GGATCGG 925 0.0 54.88483 1 TCGGAGA 605 0.0 54.105976 4 GATCCGG 630 0.0 51.965946 1 CGGAGAG 1275 0.0 51.347633 5 AACCGGA 345 0.0 50.881435 2 GGTCCGG 285 0.0 49.94439 1 TTCGGAG 360 0.0 48.758545 3 GTTCGGA 300 0.0 47.4435 2 AAAGCGG 940 0.0 47.431126 8 >>END_MODULE