FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5187311
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC139550.2690218496635347No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG131260.25304054451333263No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA94150.18150058864795265No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA90340.17415574273453047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69930.13480973089911133No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69700.1343663412507945No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA65660.12657810568905548No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT59560.1148186411032614No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC57890.11159924670026533No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG56860.10961363218823779No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT55040.10610507062329597No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT54580.10521829132666231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAGA20600.077.8396764
GAGCGGC14650.073.493259
AGAGCGG15950.070.1802148
GAGAGCG20100.069.378217
TCGGGAA7450.063.0420723
CGGAAAG10700.062.0720065
ATTCGGA3000.061.676552
TCGGGAG26200.060.296833
ACCGGAA4050.055.0546953
GAACCGG3450.055.011211
AAGCGGC6650.054.919729
GGATCGG9250.054.884831
TCGGAGA6050.054.1059764
GATCCGG6300.051.9659461
CGGAGAG12750.051.3476335
AACCGGA3450.050.8814352
GGTCCGG2850.049.944391
TTCGGAG3600.048.7585453
GTTCGGA3000.047.44352
AAAGCGG9400.047.4311268
Produced by FastQC (version 0.11.2)