FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34274-2.3420000003583a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34274-2.3420000003583a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478203
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT66530.14856405571609863Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8150.057.0315482
GAGCGGC4600.053.6055269
CGAGGCG1650.051.744531
CGGTGCG752.419256E-950.594651
CGGGAGA7650.048.3582044
CGACGGG857.3214323E-944.6423381
CGGGGCG1750.043.3668441
CGCGGCA459.6956926E-442.1622121
CGCGACC500.001627649237.9459881
CGAGCGC500.001627649237.9459881
TCGCGGG756.9979324E-637.943442
GATCGGG14750.037.3028341
GAGAGCG8600.036.9449427
CGGAAAG4400.036.6490945
GTCGCGG651.3399644E-436.486531
AGAGCGG7150.036.477958
ACGCGCC550.00259933734.4940342
CGACGGC702.0715137E-433.8803441
GATCCGG1850.033.3309361
GGTCCGG2450.032.9123341
Produced by FastQC (version 0.11.2)