FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5064296
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT79960.15788966521704104Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC46250.077.828019
ATCGGGA79950.073.981432
TCGGGAG74700.072.257583
CGGGAGA75150.071.3199844
ATTCGGA11050.069.968092
AGAGCGG53250.069.291318
GATCGGG90250.067.909221
ATCCGGA14000.066.40532
TCGGAGA18150.066.377194
ATCGGAG15000.063.2412643
GATCCGG15700.062.845921
CGGAAAG24200.062.326625
GAGAGCG69450.061.6643757
GTTCGGA10750.060.448682
AAGCGGC14400.059.9397359
CGGAGAG36500.059.5160945
AACCGGA7800.056.5539632
AATCGGA10950.056.312392
GAGGGGC81900.056.1107189
GGATCGG29450.055.7319451
Produced by FastQC (version 0.11.2)