##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3387621 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93646868997447 33.0 33.0 33.0 27.0 33.0 2 31.820894958438384 33.0 33.0 33.0 33.0 33.0 3 31.7910448069604 33.0 33.0 33.0 33.0 33.0 4 35.46075490735239 37.0 37.0 37.0 33.0 37.0 5 35.45421580513286 37.0 37.0 37.0 33.0 37.0 6 35.54004742561225 37.0 37.0 37.0 33.0 37.0 7 35.42252719533856 37.0 37.0 37.0 33.0 37.0 8 35.401892360450006 37.0 37.0 37.0 33.0 37.0 9 35.24192848019303 37.0 37.0 37.0 33.0 37.0 10-11 35.204077876480284 37.0 37.0 37.0 33.0 37.0 12-13 35.2354490068399 37.0 37.0 37.0 33.0 37.0 14-15 36.96266539261623 40.0 37.0 40.0 33.0 40.0 16-17 36.90049211526319 40.0 37.0 40.0 33.0 40.0 18-19 36.874124496217256 40.0 37.0 40.0 33.0 40.0 20-21 36.81855792014514 40.0 37.0 40.0 33.0 40.0 22-23 37.00009593753256 40.0 37.0 40.0 33.0 40.0 24-25 36.695981043924334 40.0 37.0 40.0 33.0 40.0 26-27 36.59308375996016 40.0 37.0 40.0 33.0 40.0 28-29 36.41830033525002 40.0 37.0 40.0 33.0 40.0 30-31 36.429833207433774 40.0 37.0 40.0 33.0 40.0 32-33 36.316587806014894 40.0 37.0 40.0 33.0 40.0 34-35 36.27592918452211 40.0 37.0 40.0 33.0 40.0 36-37 36.14573058792587 40.0 37.0 40.0 33.0 40.0 38-39 36.07427897040431 40.0 37.0 40.0 33.0 40.0 40-41 36.1062031437401 40.0 37.0 40.0 27.0 40.0 42-43 35.94674655163609 37.0 37.0 40.0 27.0 40.0 44-45 35.75092373084238 37.0 37.0 40.0 27.0 40.0 46-47 35.62042713751036 37.0 37.0 40.0 27.0 40.0 48-49 35.4813355744341 37.0 37.0 40.0 27.0 40.0 50-51 34.39902766572766 37.0 35.0 38.5 24.5 38.5 52-53 34.8326360888659 37.0 35.0 38.5 27.0 40.0 54-55 35.55864203817369 37.0 37.0 40.0 27.0 40.0 56-57 35.63891474282394 37.0 37.0 40.0 27.0 40.0 58-59 35.442012993779414 37.0 37.0 40.0 27.0 40.0 60-61 35.237894380746845 37.0 37.0 40.0 27.0 40.0 62-63 34.977289224502975 37.0 33.0 40.0 27.0 40.0 64-65 34.76869003350729 37.0 33.0 40.0 27.0 40.0 66-67 34.56143736268018 37.0 33.0 40.0 27.0 40.0 68-69 34.41170765560847 37.0 33.0 40.0 27.0 40.0 70-71 34.107989353000235 37.0 33.0 38.5 27.0 40.0 72-73 33.95399765794343 37.0 33.0 37.0 27.0 40.0 74-75 33.74827718330947 37.0 33.0 37.0 27.0 40.0 76-77 33.46286390951054 37.0 33.0 37.0 22.0 40.0 78-79 33.09339002208334 37.0 33.0 37.0 22.0 37.0 80-81 32.71727415788248 37.0 33.0 37.0 22.0 37.0 82-83 32.39857174105368 37.0 33.0 37.0 22.0 37.0 84-85 32.15551592105493 37.0 33.0 37.0 22.0 37.0 86-87 31.894607897400565 37.0 33.0 37.0 18.5 37.0 88-89 31.7071363650184 33.0 33.0 37.0 15.0 37.0 90-91 31.540760020084893 33.0 33.0 37.0 15.0 37.0 92-93 31.34184786314644 33.0 33.0 37.0 15.0 37.0 94-95 31.168168162849383 33.0 33.0 37.0 15.0 37.0 96-97 30.979534605553575 33.0 33.0 37.0 15.0 37.0 98-99 30.81786702231448 33.0 30.0 37.0 15.0 37.0 100-101 29.432620856937657 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13426194347489684 2306 2 0.24659172262506246 2306 3 0.2755408607529226 2306 4 0.35427560151071447 2306 5 0.31522454692871094 2306 6 0.23904177273742988 2306 7 0.38443417431265914 2306 8 0.31086890425562785 2306 9 0.3925513545233059 2306 10-11 0.3519571731355313 2306 12-13 0.3233056901539513 2306 14-15 0.5593317448184294 2306 16-17 0.5263854941695243 2306 18-19 0.4720786802484582 2306 20-21 0.4086870429610556 2306 22-23 0.20747278610051723 2306 24-25 0.2825977751516078 2306 26-27 0.20311374812779093 2306 28-29 0.32561243112029814 2306 30-31 0.4621028832166587 2306 32-33 0.37152481245055213 2306 34-35 0.35337379124520396 2306 36-37 0.4700431122111297 2306 38-39 0.2962356024287729 2306 40-41 0.22098621564922638 2306 42-43 0.4479334494791658 2306 44-45 0.4712517003112069 2306 46-47 0.442154316113367 2306 48-49 0.5017092600473703 2306 50-51 0.31861678379816993 2306 52-53 0.3985503693843313 2306 54-55 0.4000649918314281 2306 56-57 0.45058246045806527 2306 58-59 0.5739825410887534 2306 60-61 0.27362623388423657 2306 62-63 0.35316651279854483 2306 64-65 0.3611341147520193 2306 66-67 0.34106275645071804 2306 68-69 0.31390792482267216 2306 70-71 0.43332607034377446 2306 72-73 0.34496952904923006 2306 74-75 0.2982237003514854 2306 76-77 0.28964508974191716 2306 78-79 0.2854070572563856 2306 80-81 0.35802546861798135 2306 82-83 0.29939131568497857 2306 84-85 0.4113168333473496 2306 86-87 0.4894261481502866 2306 88-89 0.5407963516042713 2306 90-91 0.24497719982622357 2306 92-93 0.1846536520030959 2306 94-95 0.4838508374241215 2306 96-97 0.2814981414629827 2306 98-99 0.3589898981040456 2306 100-101 0.09691020214044954 2307 1 0.08842934958796178 2307 2 0.10867757576418313 2307 3 0.05119680658449255 2307 4 0.17153393905320513 2307 5 0.1394288457287587 2307 6 0.1331177832762478 2307 7 0.20340063302425193 2307 8 0.16295431372691382 2307 9 0.15237262464193435 2307 10-11 0.1533462800663017 2307 12-13 0.08709394797273973 2307 14-15 0.2663168682946022 2307 16-17 0.19846206441237513 2307 18-19 0.11699595326116707 2307 20-21 0.24576368146050953 2307 22-23 0.08053259252482547 2307 24-25 0.2741693660762152 2307 26-27 0.14875889235396755 2307 28-29 0.19901949294711585 2307 30-31 0.34151652497877194 2307 32-33 0.5046827547873818 2307 34-35 0.28601392202483567 2307 36-37 0.41910961801976754 2307 38-39 0.34275417504696293 2307 40-41 0.2232885957565287 2307 42-43 0.4573861340108607 2307 44-45 0.37777728629402674 2307 46-47 0.39248612846235176 2307 48-49 0.3723131090364902 2307 50-51 0.07264989507006447 2307 52-53 0.04660061988601427 2307 54-55 0.1633323665464559 2307 56-57 0.29062100836736704 2307 58-59 0.27909060833513877 2307 60-61 0.3869684348862492 2307 62-63 0.393346181208031 2307 64-65 0.4143418332072386 2307 66-67 0.261870180720031 2307 68-69 0.15774751159727884 2307 70-71 0.07435442413132876 2307 72-73 0.06635338022255866 2307 74-75 0.006341030017161131 2307 76-77 0.05229815514537961 2307 78-79 0.05994700783860907 2307 80-81 0.10097840228622346 2307 82-83 0.15273477943389935 2307 84-85 0.19548565578465826 2307 86-87 0.2451720732318563 2307 88-89 0.21215580104662024 2307 90-91 0.09407936970322694 2307 92-93 0.036773189769466086 2307 94-95 -0.013742264564875484 2307 96-97 0.16427128727271167 2307 98-99 0.25384019433764493 2307 100-101 0.1443732753251652 2201 1 0.2821046279679109 2201 2 0.25956436740245437 2201 3 0.19617448068182242 2201 4 0.20823406887647877 2201 5 0.2718692071986908 2201 6 0.30619903537700566 2201 7 0.332147699665839 2201 8 0.28667901591972367 2201 9 0.35039224152451 2201 10-11 0.288102395020033 2201 12-13 0.2565226677487473 2201 14-15 0.5443530869836053 2201 16-17 0.4452006786645697 2201 18-19 0.532379548656607 2201 20-21 0.6030891369983493 2201 22-23 0.46418542165456955 2201 24-25 0.2884135964955661 2201 26-27 0.27485735991511717 2201 28-29 0.2565042595273326 2201 30-31 0.4777575321730154 2201 32-33 0.48564358192506774 2201 34-35 0.47400846852222855 2201 36-37 0.5058482746726654 2201 38-39 0.37302327309899397 2201 40-41 0.3682644389139753 2201 42-43 0.5220928983893245 2201 44-45 0.5106910902701358 2201 46-47 0.48209858980060716 2201 48-49 0.4731947162239507 2201 50-51 0.3779922692632951 2201 52-53 0.433575737605814 2201 54-55 0.5684769606868301 2201 56-57 0.6695556981637552 2201 58-59 0.7688628818151102 2201 60-61 0.6840233006378824 2201 62-63 0.707939138218336 2201 64-65 0.6737131616909551 2201 66-67 0.5389489142184445 2201 68-69 0.4935173119593088 2201 70-71 0.49878710259207537 2201 72-73 0.34017689534959317 2201 74-75 0.17500441692444468 2201 76-77 0.29524537473789536 2201 78-79 0.40346871854548993 2201 80-81 0.4638299234696177 2201 82-83 0.3808798137707683 2201 84-85 0.43981195856583355 2201 86-87 0.35435978735546314 2201 88-89 0.5621994159617998 2201 90-91 0.5254515993948239 2201 92-93 0.4120196920961128 2201 94-95 0.4100850473532702 2201 96-97 0.48055988733993615 2201 98-99 0.39096400364853423 2201 100-101 0.35865703477281485 1101 1 5.419946585583091E-4 1101 2 -0.10822475622249073 1101 3 -0.15384987886301715 1101 4 -0.05813831996245966 1101 5 -0.07524528470477776 1101 6 0.06058962629428066 1101 7 0.09839837589429123 1101 8 -0.14485142738313783 1101 9 -0.00672753764285261 1101 10-11 0.026488969250934247 1101 12-13 -0.05761826081463539 1101 14-15 0.03338516361862531 1101 16-17 0.0016953316508576677 1101 18-19 0.046352071226209546 1101 20-21 -0.01137484917645537 1101 22-23 0.05635344497709838 1101 24-25 -0.15055374264888144 1101 26-27 0.052950332688595836 1101 28-29 -4.115527774928296E-5 1101 30-31 0.06790844559637321 1101 32-33 0.1481871133339041 1101 34-35 0.13624942067912826 1101 36-37 0.09658306817147633 1101 38-39 -0.02931869882233684 1101 40-41 0.03593377349836402 1101 42-43 0.04239243397486092 1101 44-45 -0.00965032126249099 1101 46-47 0.05226003689715952 1101 48-49 0.011430349795112704 1101 50-51 0.17979749708376858 1101 52-53 0.22635959269188533 1101 54-55 0.2567168329209366 1101 56-57 0.30188326544018196 1101 58-59 0.3212359032191685 1101 60-61 0.3949696391650832 1101 62-63 0.44244929347596695 1101 64-65 0.37186350451197114 1101 66-67 0.41428055980357925 1101 68-69 0.2647538644181253 1101 70-71 0.2807189591911339 1101 72-73 0.17877184497418597 1101 74-75 0.17263382977259312 1101 76-77 0.2506005934171611 1101 78-79 0.29797350483006113 1101 80-81 0.3041999195918379 1101 82-83 0.27767424406552976 1101 84-85 0.11312416213605303 1101 86-87 0.27807670663395356 1101 88-89 0.32538318974725655 1101 90-91 0.20713354510793636 1101 92-93 0.08278256971587084 1101 94-95 0.10816168169829155 1101 96-97 0.2213993536837613 1101 98-99 0.22097770355187762 1101 100-101 0.11235667256289616 2304 1 -0.1138759169715371 2304 2 -0.19171876494779383 2304 3 -0.056178389566159836 2304 4 -0.10432953461399563 2304 5 0.019304712831043958 2304 6 -0.042301835349370265 2304 7 0.015342476580237019 2304 8 -0.060268041012058404 2304 9 -0.0035597858818761097 2304 10-11 3.045242817378835E-4 2304 12-13 -0.13544436131130766 2304 14-15 -0.005233772806526815 2304 16-17 -0.0028388286379339434 2304 18-19 -0.14767529413375513 2304 20-21 -0.10000507848395301 2304 22-23 -0.1556002750676626 2304 24-25 -0.14844997088665224 2304 26-27 -0.22602535904201915 2304 28-29 -0.22832072471506848 2304 30-31 -0.1284472641726211 2304 32-33 0.007128732911617419 2304 34-35 0.01897329111121593 2304 36-37 0.018934297674597644 2304 38-39 -0.2051185659684478 2304 40-41 -0.20173264533966773 2304 42-43 -0.018439239188872136 2304 44-45 -0.057112249736071874 2304 46-47 -0.08338879588443149 2304 48-49 -0.10231422183577621 2304 50-51 -0.10734195081371212 2304 52-53 -0.17115878507573967 2304 54-55 0.12292293543128352 2304 56-57 0.18267011175690584 2304 58-59 0.14043836872598803 2304 60-61 0.05338489250812728 2304 62-63 0.04507324835523008 2304 64-65 -0.06885082849895241 2304 66-67 0.04667947641252823 2304 68-69 0.049941246141429474 2304 70-71 0.03489014477446517 2304 72-73 0.11313676622787483 2304 74-75 0.1596402121408218 2304 76-77 0.19109579613422767 2304 78-79 0.10820999622978178 2304 80-81 0.06833446604579763 2304 82-83 -0.08732615050505643 2304 84-85 -0.05379983207933492 2304 86-87 -0.06617592678457385 2304 88-89 0.03410415214876039 2304 90-91 -0.02751871726306021 2304 92-93 -0.13269703914827247 2304 94-95 -0.12080611862057111 2304 96-97 0.06311018151704317 2304 98-99 -0.023029963513995 2304 100-101 -0.0349924631461036 2202 1 -0.14435494483827682 2202 2 -0.2342121463086535 2202 3 -0.18177267114784712 2202 4 -0.20865880379060542 2202 5 -0.133750142815245 2202 6 -0.2384233477117732 2202 7 -0.05184791848714809 2202 8 -0.26101588951810584 2202 9 -0.17748006584319853 2202 10-11 -0.26302791958666916 2202 12-13 -0.3090863926630689 2202 14-15 -0.14854415543930344 2202 16-17 -0.19407327262123886 2202 18-19 -0.2805126970823011 2202 20-21 -0.08380679473962971 2202 22-23 -0.19263941993573042 2202 24-25 -0.3392354319471451 2202 26-27 -0.2559544667189968 2202 28-29 -0.23240116742162797 2202 30-31 -0.08412540153643988 2202 32-33 -0.09746898644304736 2202 34-35 -0.016678973024426114 2202 36-37 0.07550674996159756 2202 38-39 0.04128970487415984 2202 40-41 -0.09084285648647494 2202 42-43 -0.0018970036539016633 2202 44-45 -0.003986026609943849 2202 46-47 -0.11862328443046266 2202 48-49 -0.06549225316176432 2202 50-51 -0.08854392843249315 2202 52-53 -0.11675769205399433 2202 54-55 -0.06934665243863947 2202 56-57 0.20717531781121323 2202 58-59 0.15248896299415549 2202 60-61 0.16757346426189912 2202 62-63 0.1435760748671484 2202 64-65 0.13856203391549826 2202 66-67 0.14929046311188898 2202 68-69 0.019287792771493173 2202 70-71 0.04356833368070312 2202 72-73 0.0010854605667489636 2202 74-75 0.10811542855193323 2202 76-77 0.018452735007585375 2202 78-79 0.007356217283465583 2202 80-81 -0.03582608219389272 2202 82-83 -0.08860504715335082 2202 84-85 0.08511608825109107 2202 86-87 0.10135852745352736 2202 88-89 0.03337114893756166 2202 90-91 -0.19073984826513524 2202 92-93 -0.07595841620104338 2202 94-95 0.04973692387562778 2202 96-97 -0.07673685825599819 2202 98-99 -0.020894777910225315 2202 100-101 0.05913885355369786 1102 1 -0.19838768486027547 1102 2 -0.30136469424209267 1102 3 -0.2906476205200157 1102 4 -0.26552082745701 1102 5 -0.27720937260868794 1102 6 -0.2080059854643963 1102 7 -0.17756529008828892 1102 8 -0.22565520049117538 1102 9 -0.16803875288785974 1102 10-11 -0.11245671784898548 1102 12-13 -0.24391702730453346 1102 14-15 -0.3439617089089708 1102 16-17 -0.26032644218841483 1102 18-19 -0.266623449811604 1102 20-21 -0.3507616464640222 1102 22-23 -0.2272895914137436 1102 24-25 -0.25201531342617756 1102 26-27 -0.2358080457322842 1102 28-29 -0.273295092496582 1102 30-31 -0.15961587521019283 1102 32-33 -0.11306431674846351 1102 34-35 -0.07100678287481799 1102 36-37 -0.16973855368788549 1102 38-39 -0.1334870142949356 1102 40-41 -0.19970870552038633 1102 42-43 -0.09623681212749347 1102 44-45 -0.09841902715504602 1102 46-47 -0.20181016759456583 1102 48-49 -0.13096239045937352 1102 50-51 0.020777916664549423 1102 52-53 0.030958249757290446 1102 54-55 0.05447652407244874 1102 56-57 0.16164482799183588 1102 58-59 0.25147357259425007 1102 60-61 0.21014720810419618 1102 62-63 0.27730439359924475 1102 64-65 0.27734858559689 1102 66-67 0.25458846117570744 1102 68-69 0.24333817823417547 1102 70-71 0.08650073452503193 1102 72-73 0.12382074347667782 1102 74-75 0.2682930740663423 1102 76-77 0.22723540234206752 1102 78-79 0.20271698003465843 1102 80-81 0.2010110272806429 1102 82-83 0.2784030703483751 1102 84-85 0.18651246028029078 1102 86-87 0.10959289364838298 1102 88-89 0.09645642164129242 1102 90-91 0.00556302988387003 1102 92-93 0.06176869483552494 1102 94-95 4.327887280020093E-4 1102 96-97 0.14198706466713062 1102 98-99 -0.032796234839821636 1102 100-101 -0.01219276553701576 2305 1 0.005129343572331635 2305 2 -0.11397499279725665 2305 3 -0.10829223271983324 2305 4 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-0.6364482453498752 1213 76-77 -0.635901059238769 1213 78-79 -0.7236335937434646 1213 80-81 -0.7156012525419513 1213 82-83 -0.7588057844950455 1213 84-85 -0.7762470259432632 1213 86-87 -0.7429334830470964 1213 88-89 -0.6784842886915428 1213 90-91 -0.7044644875186883 1213 92-93 -0.6652678922970061 1213 94-95 -0.6003309121988067 1213 96-97 -0.5577923514091019 1213 98-99 -0.5625787293469351 1213 100-101 -0.391187642456444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3737.0 3 324.0 4 342.0 5 289.0 6 615.0 7 1924.0 8 8830.0 9 18786.0 10 26239.0 11 19064.0 12 11793.0 13 9173.0 14 7968.0 15 7772.0 16 8027.0 17 8811.0 18 9833.0 19 11039.0 20 12812.0 21 14500.0 22 16485.0 23 18461.0 24 21099.0 25 24903.0 26 30052.0 27 36463.0 28 44846.0 29 52742.0 30 61227.0 31 76915.0 32 101105.0 33 138668.0 34 202823.0 35 319446.0 36 550987.0 37 958858.0 38 547008.0 39 3655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.436863057207056 29.0542217777666 16.220526255837793 18.288388909188544 2 32.12016799585958 26.427655577121058 27.1458073977716 14.306369029247767 3 35.27383754214108 23.56004337258858 23.361050172854263 17.80506891241607 4 30.40360421232997 23.76824079281311 27.154985094372723 18.673169900484194 5 33.123913824291165 21.554992369772823 27.00698051880811 18.314113287127903 6 31.36503126488892 21.950437826816486 28.446106907276597 18.238424001017993 7 34.133471722854864 22.521563201957967 23.125409783681555 20.219555291505614 8 29.54048929788454 28.210109537193745 22.74747930147297 19.50192186344874 9 29.772556777978405 24.36347372132703 25.117259511509417 20.746709989185145 10-11 25.974561814860873 29.742596870559307 27.844574691154545 16.43826662342527 12-13 26.50669135198726 28.17565747520115 27.40117920924433 17.91647196356726 14-15 25.114713690219716 28.217430386713936 28.062753096023428 18.60510282704292 16-17 25.57723527928186 28.0829432486944 28.13428723789012 18.20553423413362 18-19 25.027495704812974 28.100181391782584 28.517187255955285 18.355135647449156 20-21 24.4140257573696 28.945465247664742 28.099536201704932 18.54097279326073 22-23 25.34304205923555 28.61716448528352 27.52057128705918 18.519222168421752 24-25 23.545557828575344 29.87084728258927 27.86337079291702 18.72022409591837 26-27 23.124992501258678 30.14916162961091 27.78597310999502 18.939872759135394 28-29 23.426791914900498 29.850903223328057 27.731528800018868 18.99077606175258 30-31 24.040617095779623 28.611113949306667 28.268886797015362 19.079382157898344 32-33 22.991270244339525 29.41210894327348 28.44939722551286 19.147223586874134 34-35 24.18575584987275 28.641197433816412 28.00476292492304 19.1682837913878 36-37 23.058284901487962 28.987942970807545 28.573536765604686 19.380235362099814 38-39 22.73474584093311 29.894982718365736 28.014752170149244 19.355519270551916 40-41 24.063747782522523 28.47109956212737 27.94161639050895 19.52353626484115 42-43 23.804920497030682 28.419769155279095 28.152026636385468 19.62328371130475 44-45 22.767656379444443 28.99422163340912 28.5365249756512 19.701597011495238 46-47 23.133348686899584 29.146285400270138 27.88658160553843 19.833784307291847 48-49 21.944974620462883 29.099320233576808 28.625800061916852 20.32990508404346 50-51 21.46612790197178 28.4528558165125 29.34153001900755 20.739486262508166 52-53 23.334173271296503 28.4103976414542 28.092071035035787 20.16335805221351 54-55 23.277076449766426 28.414919668690857 28.461149678101332 19.84685420344138 56-57 23.502526847476577 28.264710354309923 28.300573182843813 19.932189615369683 58-59 23.078182692233597 28.545668909078298 27.986710910283975 20.38943748840413 60-61 21.966311915701134 28.3666716881948 28.013564939838687 21.65345145626538 62-63 22.769048478582704 28.471442570780177 28.144243159049093 20.615265791588026 64-65 21.879553084044463 28.96215048125594 28.846252113386374 20.312044321313227 66-67 21.54709889885904 28.840454250271375 28.32595348473429 21.286493366135296 68-69 21.25772152948678 28.543986705009246 29.829346885060392 20.368944880443582 70-71 21.172774307823456 29.618482384575053 28.9225780724915 20.286165235109994 72-73 21.17698426376188 30.659398776327002 27.93774499962393 20.225871960287186 74-75 21.005799225151023 30.842389131502213 28.01152796640939 20.140283676937372 76-77 21.128501708085274 30.792640713603358 27.854517840750386 20.224339737560978 78-79 21.066447081962163 30.936843726581497 27.845379390830168 20.151329800626172 80-81 21.12408307653206 30.71636964808919 27.8815976055645 20.27794966981424 82-83 21.245813571494597 30.655085695865598 27.721845157253277 20.377255575386528 84-85 21.18990462981074 30.48671989378286 27.909057500696456 20.414317975709942 86-87 21.45348886317372 30.13117254793698 27.83629386242761 20.579044726461692 88-89 21.47360968552939 30.21679560247615 27.77601694665363 20.533577765340826 90-91 21.552584725419262 29.941897025168572 27.832692229921452 20.67282601949071 92-93 21.634021407928046 29.930193341960802 27.736601976458207 20.69918327365294 94-95 21.536575138252108 29.817427850374408 27.914044116297017 20.731952895076468 96-97 21.68620386064135 29.701658865857926 27.78200090188752 20.8301363716132 98-99 21.691061174032424 29.767165222698484 27.782644087581726 20.75912951568737 100-101 21.896050119063 29.660065949878568 27.669509204417363 20.774374726641074 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2701.0 1 1700.0 2 523.0 3 295.5 4 255.0 5 269.0 6 312.0 7 416.0 8 571.5 9 765.5 10 945.0 11 1058.5 12 1083.5 13 1039.0 14 967.0 15 887.0 16 840.0 17 891.5 18 1051.5 19 1387.0 20 1936.0 21 2871.0 22 4232.5 23 5958.0 24 8402.5 25 11580.5 26 15622.0 27 20466.5 28 26158.0 29 32822.5 30 40312.5 31 48926.0 32 58577.5 33 69116.0 34 80852.0 35 93420.5 36 106333.5 37 119293.5 38 131373.0 39 141526.5 40 150240.0 41 156714.5 42 159594.5 43 160301.5 44 159870.0 45 158030.5 46 154622.0 47 149923.0 48 143938.5 49 135764.0 50 126035.5 51 115454.5 52 102427.5 53 87909.5 54 74356.0 55 61865.5 56 51045.5 57 42109.5 58 33996.0 59 27453.0 60 22274.0 61 17676.0 62 13837.5 63 10708.0 64 8147.5 65 6197.0 66 4738.0 67 3620.0 68 2772.0 69 2086.0 70 1596.0 71 1240.0 72 980.0 73 777.0 74 628.0 75 474.0 76 365.5 77 287.0 78 209.0 79 179.5 80 156.5 81 121.0 82 91.5 83 75.5 84 70.0 85 56.5 86 44.0 87 34.5 88 26.5 89 25.5 90 22.5 91 16.0 92 12.5 93 10.0 94 7.0 95 5.5 96 5.0 97 4.5 98 3.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22747526951804822 2 0.2435927749887015 3 0.22452334543917396 4 0.21708449676041092 5 0.22561555734835745 6 0.24887671908988637 7 0.22992536650351383 8 0.21572661168412877 9 0.20914382098823925 10-11 0.19487126806688235 12-13 0.22064156527545437 14-15 0.21718781410317153 16-17 0.2207596422386093 18-19 0.20937997491454916 20-21 0.2019411262357861 22-23 0.17072452910169114 24-25 0.49950983300670293 26-27 0.3558396880878941 28-29 0.4887057908780233 30-31 0.32655660122546176 32-33 0.3756913775183233 34-35 0.37796435905905645 36-37 0.3847390248200729 38-39 0.3777134455123522 40-41 0.384030563041143 42-43 0.43661909050628744 44-45 0.3903771998107226 46-47 0.40771975377410874 48-49 0.4056386472985024 50-51 0.41991120021985934 52-53 0.42377822076318455 54-55 0.3893440263831166 56-57 0.4030557137294875 58-59 0.4007236937071768 60-61 0.35879161216676836 62-63 0.3227633787841084 64-65 0.34887314726175095 66-67 0.3605184877529098 68-69 0.35167747513668146 70-71 0.32280765764529146 72-73 0.31216597134094987 74-75 0.3236194367669819 76-77 0.3300103523977446 78-79 0.34097675035076236 80-81 0.34814992586242677 82-83 0.3668946437632781 84-85 0.39558734580993565 86-87 0.3978455677302744 88-89 0.3916465271646385 90-91 0.3636918061376996 92-93 0.3418918468152134 94-95 0.319368666093403 96-97 0.33558948890681695 98-99 0.3652563258995029 100-101 0.38189041808395924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3387621.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.37614939029055 #Duplication Level Percentage of deduplicated Percentage of total 1 97.69903111233958 93.18157386657144 2 1.7366672620121304 3.312732724457916 3 0.27453554672083597 0.7855242995097461 4 0.10470884931923594 0.39946907420627487 5 0.04717477175039811 0.2249674038959414 6 0.0327990314240741 0.18769471925755954 7 0.02064092498559616 0.13780563614860003 8 0.008219167375152029 0.06271300283490416 9 0.02194368954171198 0.18836141507140305 >10 0.05192554477114469 0.8923261756741026 >50 0.0018806356956332353 0.12141327594696563 >100 4.42575876267668E-4 0.0590999431245166 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.0888188255268883E-5 0.44631846330063635 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 15150 0.44721649794944596 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0032175972459729113 0.0 0.0 2 0.004368847636733861 0.0 0.0 3 0.006641829177467019 0.0 2.9519240788742308E-5 4 0.00897384919977766 0.0 2.9519240788742308E-5 5 0.011955292519440634 0.0 5.9038481577484616E-5 6 0.02202135362840176 0.0 5.9038481577484616E-5 7 0.03155606840316553 0.0 5.9038481577484616E-5 8 0.04041184063978822 0.0 5.9038481577484616E-5 9 0.045548188537029376 0.0 5.9038481577484616E-5 10-11 0.05487626862627195 0.0 7.379810197185576E-5 12-13 0.06724483051675498 0.0 8.855772236622692E-5 14-15 0.07868353632239261 0.0 1.0331734276059808E-4 16-17 0.09274945455822833 0.0 1.1807696315496923E-4 18-19 0.10889647926967036 0.0 1.328365835493404E-4 20-21 0.12839393781063466 0.0 1.4759620394371153E-4 22-23 0.14967731041931787 0.0 1.4759620394371153E-4 24-25 0.1709016445464236 0.0 1.623558243380827E-4 26-27 0.19215549791431805 0.0 2.0663468552119615E-4 28-29 0.2142211304039029 1.4759620394371154E-5 2.0663468552119615E-4 30-31 0.24546724677878665 2.9519240788742308E-5 2.2139430591556732E-4 32-33 0.2801671143259532 2.9519240788742308E-5 2.509135467043096E-4 34-35 0.32236486903346034 2.9519240788742308E-5 2.8043278749305194E-4 36-37 0.37036315455595537 2.9519240788742308E-5 2.9519240788742306E-4 38-39 0.42309927822504345 2.9519240788742308E-5 3.394712690705365E-4 40-41 0.4853110781873179 2.9519240788742308E-5 3.8375013025364996E-4 42-43 0.5553159577178202 2.9519240788742308E-5 3.9850975064802113E-4 44-45 0.62808088626207 2.9519240788742308E-5 4.132693710423923E-4 46-47 0.7096277889409707 2.9519240788742308E-5 4.132693710423923E-4 48-49 0.7967833473697323 2.9519240788742308E-5 4.280289914367634E-4 50-51 0.8846621271978181 2.9519240788742308E-5 4.7230785261987687E-4 52-53 0.9787842264527229 2.9519240788742308E-5 5.018270934086192E-4 54-55 1.075769692064136 5.9038481577484616E-5 5.018270934086192E-4 56-57 1.1792789098898608 5.9038481577484616E-5 5.165867138029903E-4 58-59 1.286581350156939 5.9038481577484616E-5 5.313463341973615E-4 60-61 1.3997728789613715 5.9038481577484616E-5 5.313463341973615E-4 62-63 1.5147503218335228 5.9038481577484616E-5 5.313463341973615E-4 64-65 1.6338309391753092 5.9038481577484616E-5 5.313463341973615E-4 66-67 1.7584021353038017 5.9038481577484616E-5 5.313463341973615E-4 68-69 1.884523091573703 5.9038481577484616E-5 5.461059545917327E-4 70-71 2.017094001955945 5.9038481577484616E-5 5.75625195380475E-4 72-73 2.152188807425624 5.9038481577484616E-5 6.199040565635885E-4 74-75 2.2930103456083195 5.9038481577484616E-5 7.084617789298154E-4 76-77 2.4386582796599736 5.9038481577484616E-5 7.379810197185577E-4 78-79 2.589058811478616 5.9038481577484616E-5 7.379810197185577E-4 80-81 2.7468834323556264 5.9038481577484616E-5 7.527406401129288E-4 82-83 2.908619352637146 5.9038481577484616E-5 7.675002605072999E-4 84-85 3.0774546503283573 5.9038481577484616E-5 7.675002605072999E-4 86-87 3.257462980658108 5.9038481577484616E-5 7.675002605072999E-4 88-89 3.446282804363298 5.9038481577484616E-5 7.970195012960423E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1575 0.0 73.46773 2 GATCGGG 1760 0.0 68.44178 1 GAGCGGC 1020 0.0 60.89677 9 AAGCGGC 500 0.0 56.898968 9 TCGGGAG 1845 0.0 56.80289 3 GTTCGGA 305 0.0 49.755085 2 AGAGCGG 1430 0.0 49.075153 8 GATCCGG 465 0.0 47.93416 1 GGATCGG 800 0.0 47.42422 1 CGGGAGA 2175 0.0 46.876347 4 AAAGCGG 690 0.0 44.66838 8 GAGAGCG 1950 0.0 42.553272 7 TTCGGAA 435 0.0 42.51575 3 CCGGAAA 535 0.0 41.65994 4 AGCGGCG 1015 0.0 40.643322 10-11 CGGAAAG 1215 0.0 38.24937 5 ATCCGGA 560 0.0 37.260788 2 CGACGGG 115 2.5447662E-9 37.11461 1 TCGGAGA 550 0.0 36.21272 4 GGTTCGG 485 0.0 36.179302 1 >>END_MODULE