FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387621
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT151500.44721649794944596Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA15750.073.467732
GATCGGG17600.068.441781
GAGCGGC10200.060.896779
AAGCGGC5000.056.8989689
TCGGGAG18450.056.802893
GTTCGGA3050.049.7550852
AGAGCGG14300.049.0751538
GATCCGG4650.047.934161
GGATCGG8000.047.424221
CGGGAGA21750.046.8763474
AAAGCGG6900.044.668388
GAGAGCG19500.042.5532727
TTCGGAA4350.042.515753
CCGGAAA5350.041.659944
AGCGGCG10150.040.64332210-11
CGGAAAG12150.038.249375
ATCCGGA5600.037.2607882
CGACGGG1152.5447662E-937.114611
TCGGAGA5500.036.212724
GGTTCGG4850.036.1793021
Produced by FastQC (version 0.11.2)