FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696177
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT52050.14082117820656315Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA9500.058.4125142
GTTCGCG250.00505198956.914762
GATCGGG11650.051.3042141
GATCCGG3000.047.4360431
TCGGGAG12900.041.9122663
GAGCGGC8300.041.7055859
AGCGGCG5850.036.47664610-11
GTTCGGA3050.034.2110562
TTGTGTT312250.033.3001751
CGACGGG1605.4569682E-1132.612281
AAGCGGC3500.032.5156639
CGCCGTA8950.031.84540758-59
GGTTCGG3900.031.6240251
AGAGCGG12250.031.3556658
GTGTTTG691200.031.2671621
GTTTGAT167850.031.1941873
CGGGAGA18450.030.5897964
TCGGAAA5600.030.488814
TGTTTGA707700.030.4665932
TCGCCGT8850.030.05965856-57
Produced by FastQC (version 0.11.2)