##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718311 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13668708253029 33.0 33.0 33.0 27.0 33.0 2 30.670094040012344 33.0 33.0 33.0 27.0 33.0 3 30.53647430334498 33.0 33.0 33.0 27.0 33.0 4 33.94581267559157 37.0 37.0 37.0 27.0 37.0 5 33.98804220709109 37.0 37.0 37.0 27.0 37.0 6 34.45714526409966 37.0 37.0 37.0 27.0 37.0 7 34.08436782987671 37.0 37.0 37.0 27.0 37.0 8 34.150314662303174 37.0 37.0 37.0 27.0 37.0 9 33.688257524617306 37.0 37.0 37.0 22.0 37.0 10-11 33.70734474458588 37.0 37.0 37.0 22.0 37.0 12-13 33.7405212280714 37.0 37.0 37.0 22.0 37.0 14-15 35.358537157816016 40.0 37.0 40.0 22.0 40.0 16-17 35.25194026732041 40.0 37.0 40.0 18.5 40.0 18-19 35.28850874679166 40.0 37.0 40.0 18.5 40.0 20-21 35.2210607984149 40.0 37.0 40.0 18.5 40.0 22-23 35.95182320933844 40.0 37.0 40.0 27.0 40.0 24-25 35.38399377407515 40.0 37.0 40.0 22.0 40.0 26-27 35.12601795747433 40.0 37.0 40.0 18.5 40.0 28-29 34.84818771656371 40.0 37.0 40.0 15.0 40.0 30-31 34.91098792595844 40.0 37.0 40.0 18.5 40.0 32-33 34.715255723131015 40.0 37.0 40.0 15.0 40.0 34-35 34.84730536719309 37.0 37.0 40.0 18.5 40.0 36-37 34.603836904607306 37.0 37.0 40.0 15.0 40.0 38-39 34.50619778237295 37.0 37.0 40.0 15.0 40.0 40-41 34.877878395812694 37.0 37.0 40.0 22.0 40.0 42-43 34.686569895791905 37.0 37.0 40.0 18.5 40.0 44-45 34.27702257026514 37.0 37.0 40.0 15.0 40.0 46-47 34.094701084607316 37.0 33.0 40.0 15.0 40.0 48-49 33.959257237306545 37.0 33.0 40.0 15.0 40.0 50-51 32.96599414121489 37.0 33.0 38.5 15.0 38.5 52-53 33.407505064725854 37.0 33.0 38.5 15.0 40.0 54-55 34.12242399784277 37.0 33.0 40.0 15.0 40.0 56-57 34.388577686659104 37.0 33.0 40.0 18.5 40.0 58-59 33.9494353295116 37.0 33.0 40.0 15.0 40.0 60-61 33.66895859230235 37.0 33.0 40.0 15.0 40.0 62-63 33.32189804625004 37.0 33.0 40.0 15.0 40.0 64-65 33.084318718498366 37.0 33.0 38.5 15.0 40.0 66-67 32.92339820572407 37.0 33.0 37.0 15.0 40.0 68-69 32.93591001912584 37.0 33.0 37.0 15.0 40.0 70-71 32.58618881356843 37.0 33.0 37.0 15.0 40.0 72-73 32.68271787150182 37.0 33.0 37.0 18.5 38.5 74-75 32.735924255907435 37.0 33.0 37.0 22.0 37.0 76-77 32.517175003154534 37.0 33.0 37.0 22.0 37.0 78-79 32.1242284271373 37.0 33.0 37.0 22.0 37.0 80-81 31.68812766456818 37.0 33.0 37.0 15.0 37.0 82-83 31.347276856842356 37.0 33.0 37.0 15.0 37.0 84-85 31.09595517216389 33.0 33.0 37.0 15.0 37.0 86-87 30.858907424499993 33.0 33.0 37.0 15.0 37.0 88-89 30.724633973081076 33.0 33.0 37.0 15.0 37.0 90-91 30.600917076817183 33.0 30.0 37.0 15.0 37.0 92-93 30.43312593739274 33.0 27.0 37.0 15.0 37.0 94-95 30.29812409249714 33.0 27.0 37.0 15.0 37.0 96-97 30.129027914760307 33.0 27.0 37.0 15.0 37.0 98-99 29.99892617143513 33.0 27.0 37.0 15.0 37.0 100-101 28.748983284105464 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13752408258090298 2306 2 0.22908046209667887 2306 3 0.09105380058674939 2306 4 0.28812453025459916 2306 5 0.2795013429733757 2306 6 -0.0447768741344845 2306 7 0.41835244637422164 2306 8 0.05171755083775054 2306 9 0.3686635850894504 2306 10-11 0.3039762967507329 2306 12-13 0.23278102288790592 2306 14-15 0.5764560033097865 2306 16-17 0.49572247116785206 2306 18-19 0.5359536792779878 2306 20-21 0.5555729374300356 2306 22-23 0.28468084217731615 2306 24-25 0.560296013365658 2306 26-27 0.3007968666929557 2306 28-29 0.29612590335911193 2306 30-31 0.5439248882719951 2306 32-33 0.545422953291741 2306 34-35 0.5458080452633496 2306 36-37 0.8145436079944872 2306 38-39 0.5253414226091664 2306 40-41 0.23214506425550496 2306 42-43 0.434866537421982 2306 44-45 0.6378624147578904 2306 46-47 0.5749423665232882 2306 48-49 0.6048610205206231 2306 50-51 0.4416828813047715 2306 52-53 0.6111973288239412 2306 54-55 0.652336904560805 2306 56-57 0.6365051954780867 2306 58-59 0.8015362711781364 2306 60-61 0.8429326169904812 2306 62-63 0.7836869956612063 2306 64-65 0.6907835014336996 2306 66-67 0.587915522443268 2306 68-69 0.5097594401270982 2306 70-71 0.6439402184868541 2306 72-73 0.3630849199068251 2306 74-75 0.32283929318184335 2306 76-77 0.2648713923689954 2306 78-79 0.46973384227359816 2306 80-81 0.43049379940359245 2306 82-83 0.4392081342377949 2306 84-85 0.584442909710635 2306 86-87 0.6101877070748039 2306 88-89 0.6498999376096073 2306 90-91 0.5699559048354352 2306 92-93 0.45411034673523787 2306 94-95 0.470392239413453 2306 96-97 0.49862295789989375 2306 98-99 0.49266382369094686 2306 100-101 0.2381244011511221 2307 1 0.2573732728763112 2307 2 0.41082171246049626 2307 3 0.23532551352550968 2307 4 0.41522178330815507 2307 5 0.3828628784240067 2307 6 0.2796692795291875 2307 7 0.5636401107078939 2307 8 0.2627003640605352 2307 9 0.4643391083705737 2307 10-11 0.4504363881544293 2307 12-13 0.4404395263600236 2307 14-15 0.6252889593679569 2307 16-17 0.6418797616113352 2307 18-19 0.6434343342313369 2307 20-21 0.523440680357389 2307 22-23 0.3545548724158962 2307 24-25 0.689794689024886 2307 26-27 0.4040054619975919 2307 28-29 0.44286016934221095 2307 30-31 0.6832263315890188 2307 32-33 0.7875340473714871 2307 34-35 0.5469460294369455 2307 36-37 0.8732733083549746 2307 38-39 0.8053342283776885 2307 40-41 0.5014918216507027 2307 42-43 0.718655360953008 2307 44-45 0.7296470312373842 2307 46-47 0.7469822390800118 2307 48-49 0.7856521259505627 2307 50-51 0.5418025646673499 2307 52-53 0.48009694443208417 2307 54-55 0.7109022598426975 2307 56-57 0.5512397525150661 2307 58-59 0.8707333046183123 2307 60-61 0.87902637739748 2307 62-63 0.831143034208047 2307 64-65 0.9507608786094224 2307 66-67 0.6991082548552612 2307 68-69 0.46213369618335065 2307 70-71 0.6407849298371318 2307 72-73 0.3609792272031598 2307 74-75 0.30465047859229344 2307 76-77 0.4162989021612731 2307 78-79 0.2866389404609464 2307 80-81 0.5656381447923948 2307 82-83 0.4993830676557991 2307 84-85 0.5894875394928398 2307 86-87 0.6327714124370374 2307 88-89 0.6996277246786065 2307 90-91 0.7155692940438136 2307 92-93 0.6125561098940189 2307 94-95 0.42589578087311963 2307 96-97 0.5571469132764939 2307 98-99 0.5782843022054216 2307 100-101 0.5282778613620494 2201 1 0.09677774975085995 2201 2 0.18545286684112128 2201 3 -0.040082658001541205 2201 4 0.21282348126204909 2201 5 0.03564155122419521 2201 6 -0.05221576985126575 2201 7 0.3827339129117959 2201 8 0.02047797109558047 2201 9 0.19302504473199633 2201 10-11 0.18007820844825062 2201 12-13 0.05846681986183455 2201 14-15 0.3301306725895543 2201 16-17 0.34918326839175506 2201 18-19 0.4301914587474016 2201 20-21 0.34540750449291835 2201 22-23 0.09255737242002482 2201 24-25 0.201750673750567 2201 26-27 -0.02624826446263029 2201 28-29 0.08053595862804741 2201 30-31 0.4593908658992163 2201 32-33 0.40586062259715305 2201 34-35 0.3538467348247636 2201 36-37 0.44577136271394835 2201 38-39 0.3161222088601434 2201 40-41 0.0053254780182712125 2201 42-43 0.3808926994152273 2201 44-45 0.5430513825333989 2201 46-47 0.46438655567670395 2201 48-49 0.3740699438914348 2201 50-51 0.20502015921063332 2201 52-53 0.2553229444750471 2201 54-55 0.5132199685633054 2201 56-57 0.44119929474828723 2201 58-59 0.6163506552748714 2201 60-61 0.6597598634078636 2201 62-63 0.6044636692388394 2201 64-65 0.5489023576931586 2201 66-67 0.4431711581317046 2201 68-69 0.31538258621786497 2201 70-71 0.47212263859078263 2201 72-73 0.2397593887698477 2201 74-75 0.0601709768085783 2201 76-77 0.15367020482370464 2201 78-79 0.25946461433019863 2201 80-81 0.4561233405709757 2201 82-83 0.42007279120127805 2201 84-85 0.5529020527355897 2201 86-87 0.48576129675608115 2201 88-89 0.4678089344955403 2201 90-91 0.40082406148394156 2201 92-93 0.3980407190886801 2201 94-95 0.3497875503572061 2201 96-97 0.3646850123149967 2201 98-99 0.29096393320282843 2201 100-101 0.11968147405804075 1101 1 0.008992317106464753 1101 2 -0.037814850658506316 1101 3 -0.08895599853132907 1101 4 0.12670039176039438 1101 5 0.08211444112787092 1101 6 -0.07806121187742576 1101 7 0.42691034283020457 1101 8 0.0309107567825464 1101 9 0.16405799959214562 1101 10-11 0.10740598807851853 1101 12-13 0.13580241762904421 1101 14-15 0.28204150061502986 1101 16-17 0.2589921102336632 1101 18-19 0.1392363838345787 1101 20-21 0.01193419824989661 1101 22-23 -0.059823485766507645 1101 24-25 0.03846031191211807 1101 26-27 -0.0515304238508989 1101 28-29 0.030259498002259022 1101 30-31 0.24087082980391727 1101 32-33 0.26002363923000615 1101 34-35 0.1383117199326236 1101 36-37 0.2892128877593052 1101 38-39 0.12163408960247324 1101 40-41 0.05873333797754299 1101 42-43 0.1968786230009485 1101 44-45 0.20320620048384086 1101 46-47 0.19519387722066028 1101 48-49 0.2757086442344914 1101 50-51 0.33119782495984396 1101 52-53 0.2855726350463712 1101 54-55 0.3509486961662276 1101 56-57 0.2522654437224148 1101 58-59 0.5202852504366646 1101 60-61 0.34614999409321 1101 62-63 0.2645460516794955 1101 64-65 0.28256857411049197 1101 66-67 0.34925800162013587 1101 68-69 0.26790087406471486 1101 70-71 0.13316118859953718 1101 72-73 0.06560002688053146 1101 74-75 0.07459496850439962 1101 76-77 0.08378369026218735 1101 78-79 0.18992002551710385 1101 80-81 0.2976667538376958 1101 82-83 0.3142693787257933 1101 84-85 0.2549965598821231 1101 86-87 0.26292329420529725 1101 88-89 0.2883288123442149 1101 90-91 0.15355871391594533 1101 92-93 0.12824067500569214 1101 94-95 0.35317845532068404 1101 96-97 0.3270404003300733 1101 98-99 0.26762707699814925 1101 100-101 0.33662186971228536 2304 1 -0.01234785170923658 2304 2 -0.1295993258280994 2304 3 -0.21901658576140548 2304 4 -0.019040377749213633 2304 5 -0.02465631129302892 2304 6 -0.15154523652658725 2304 7 0.26321089691855093 2304 8 -0.1720228044001999 2304 9 -0.07774201076464493 2304 10-11 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-0.337101801093155 2304 74-75 -0.14484990747302362 2304 76-77 -0.11279339015830203 2304 78-79 -0.22701073933194138 2304 80-81 -0.14781509588526376 2304 82-83 -0.05744233998375137 2304 84-85 -0.14728063964146543 2304 86-87 -0.27649983251004784 2304 88-89 -0.28335701438666305 2304 90-91 -0.2899605238241989 2304 92-93 -0.19536504194319448 2304 94-95 -0.2773676065093582 2304 96-97 -0.15767685926290653 2304 98-99 -0.11199593579248912 2304 100-101 -0.2485536779030646 2202 1 0.025843824969062013 2202 2 0.014129260402253152 2202 3 0.07454355077819486 2202 4 0.10623788207013263 2202 5 0.21127718604038392 2202 6 0.13230234510794503 2202 7 0.3505142398503338 2202 8 0.012868529657787064 2202 9 0.1896766407479049 2202 10-11 0.1467136677446561 2202 12-13 0.09093572407035566 2202 14-15 0.2689500627381065 2202 16-17 0.24994053563899854 2202 18-19 0.1825890727884243 2202 20-21 0.29239498153832955 2202 22-23 -0.007189892974899692 2202 24-25 -0.10974281918319662 2202 26-27 -0.012857943858072929 2202 28-29 -0.007297294807941057 2202 30-31 0.3074046133297301 2202 32-33 0.3102718924931551 2202 34-35 0.2092050830795884 2202 36-37 0.34346431826582347 2202 38-39 0.338045530998599 2202 40-41 0.11397222053075495 2202 42-43 0.2676434190116339 2202 44-45 0.2559518433942216 2202 46-47 0.18488709229546885 2202 48-49 0.22637247893815982 2202 50-51 0.2027659788220859 2202 52-53 0.3370287885898975 2202 54-55 0.32239077289482765 2202 56-57 0.29784386303554555 2202 58-59 0.33807150529009533 2202 60-61 0.34563149254359615 2202 62-63 0.22838930435334248 2202 64-65 0.3243740584243042 2202 66-67 0.2629858841716981 2202 68-69 0.21247457731283959 2202 70-71 0.3869509726167095 2202 72-73 0.10493418357474127 2202 74-75 0.11434744564202504 2202 76-77 0.14665028090077215 2202 78-79 0.29240006814595176 2202 80-81 0.26233511038615376 2202 82-83 0.26806364474771627 2202 84-85 0.2816331141168682 2202 86-87 0.337696662465067 2202 88-89 0.42958325907392947 2202 90-91 0.2546230240441183 2202 92-93 0.24009806757452878 2202 94-95 0.2916419410919815 2202 96-97 0.2233596784285048 2202 98-99 0.062380641436927675 2202 100-101 0.013650184362298035 1102 1 -0.33480184055888884 1102 2 -0.5332423504738344 1102 3 -0.41225885488443126 1102 4 -0.3034782524309918 1102 5 -0.3074376020688945 1102 6 -0.48788104264558996 1102 7 -0.10650067225101623 1102 8 -0.45288973933217136 1102 9 -0.18204611777877489 1102 10-11 -0.3359454312813952 1102 12-13 -0.35017769489034123 1102 14-15 -0.4453089084055861 1102 16-17 -0.4880918456259451 1102 18-19 -0.4129945835414688 1102 20-21 -0.48888932359272985 1102 22-23 -0.4555791075660025 1102 24-25 -0.5440422318076941 1102 26-27 -0.5483561951281857 1102 28-29 -0.425905597157886 1102 30-31 -0.28902306074106576 1102 32-33 -0.24825447095893338 1102 34-35 -0.3023136896241354 1102 36-37 -0.36343841889058837 1102 38-39 -0.3245443734903617 1102 40-41 -0.4686814086870328 1102 42-43 -0.24645325677821006 1102 44-45 -0.2133114173076578 1102 46-47 -0.33845494885562033 1102 48-49 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72-73 0.12004652524055359 1213 74-75 0.03557085873949006 1213 76-77 -0.19425062671934512 1213 78-79 -0.27028058310556347 1213 80-81 -0.41858360874419986 1213 82-83 -0.5343558995397188 1213 84-85 -0.43893317560902645 1213 86-87 -0.3899108518263823 1213 88-89 -0.27980380080278877 1213 90-91 -0.2219458541868029 1213 92-93 -0.2669140294621748 1213 94-95 -0.20168374639670716 1213 96-97 -0.20412035946993612 1213 98-99 -0.11986509209116392 1213 100-101 -0.07277024565483714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2857.0 3 338.0 4 391.0 5 265.0 6 583.0 7 3415.0 8 19947.0 9 53333.0 10 68131.0 11 42506.0 12 21603.0 13 13204.0 14 9502.0 15 7814.0 16 7375.0 17 7082.0 18 7169.0 19 7742.0 20 8605.0 21 9709.0 22 10604.0 23 11787.0 24 13609.0 25 15911.0 26 19123.0 27 23187.0 28 28318.0 29 34296.0 30 41723.0 31 53555.0 32 72331.0 33 102272.0 34 155307.0 35 259712.0 36 493835.0 37 825299.0 38 264950.0 39 921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.328597358172374 23.841006618395017 19.945662132611975 14.88473389082063 2 33.34169170798838 25.391254903016424 27.85868397636891 13.408369412626284 3 37.173090472298206 20.756853970364258 25.627494854061062 16.44256070327648 4 30.624195405943045 18.93758193403398 30.36838426453652 20.069838395486457 5 34.898618456174916 16.94990428886182 28.860558162582105 19.290919092381156 6 33.36056103571967 17.128486605629902 31.86902381127109 17.641928547379337 7 37.21087745624886 18.278049490914253 25.067518614859075 19.443554437977813 8 30.212269454654038 29.745335638633186 21.530640458012453 18.51175444870032 9 32.28742133542516 26.24440427251119 22.28986900542026 19.17830538664339 10-11 27.36578536457534 30.017615967485927 26.370121078217366 16.246477589721373 12-13 29.331973428396413 25.394123271462327 26.275007654534498 18.99889564560676 14-15 26.9977804947869 25.47846733404816 27.58084991510729 19.94290225605765 16-17 28.02973815975044 25.960059716690427 28.09913973384819 17.911062389710946 18-19 27.640638886810255 26.053890822738733 28.46742881198298 17.838041478468035 20-21 25.988701710335178 27.790578394140457 28.05779407639652 18.162925819127842 22-23 28.455322150114153 26.088536193155942 27.17353494768276 18.282606709047148 24-25 25.733917318406014 28.479328677202826 27.354751336491606 18.43200266789956 26-27 23.164746566107112 31.005697082794587 27.47118003353831 18.35837631755999 28-29 24.45290194533816 29.894965527910642 27.070289201061897 18.5818433256893 30-31 26.617762138071154 27.651724620857976 27.19888167609828 18.531631564972592 32-33 23.616475540968985 29.514603864161742 28.646018519236442 18.22290207563283 34-35 27.06086705452016 27.819337611610646 27.362058417278618 17.757736916590577 36-37 24.50178633376665 28.962035861081926 28.964103654806944 17.572074150344484 38-39 23.165748315463837 31.316948301137966 27.517084433850194 18.000218949548007 40-41 27.423093127167686 26.994494216360938 26.939310837321468 18.64310181914991 42-43 27.473053834696685 26.841371325168158 27.136819040029525 18.548755800105628 44-45 24.922295137782342 28.153569253800683 28.32491322941666 18.59922237900032 46-47 24.925697713886613 28.788677908684733 27.649757841213418 18.635866536215232 48-49 22.015104251690254 29.65858467075051 28.666315553877965 19.65999552368128 50-51 20.485552604764322 28.09565735015746 30.29445547374236 21.124334571335854 52-53 24.997792797834688 27.363987400292643 27.401001903550252 20.237217898322417 54-55 26.69719074019401 27.078390850311102 27.283060191619246 18.94135821787564 56-57 27.270382070392635 26.983233280600132 26.578732043275078 19.167652605732155 58-59 26.022373066639094 27.350963171967557 26.80325144401932 19.823412317374025 60-61 22.737979324965 27.403198253115512 26.80744124061285 23.05138118130664 62-63 24.52403788138505 27.430920866904053 26.668842697338242 21.376198554372657 64-65 22.793207190723074 28.36625196396112 28.502310428497417 20.33823041681839 66-67 21.327349439443715 28.411190526379134 27.946505996876137 22.314954037301014 68-69 20.94780830771877 27.520522717935407 31.781577522331457 19.750091452014363 70-71 20.314635425767282 30.503347674138194 30.052642814692167 19.12937408540236 72-73 19.984797917462284 34.046908756141256 26.729227664100392 19.239065662296067 74-75 20.296124494435098 34.13478286540903 26.284799102530037 19.28429353762584 76-77 20.529424573006008 33.60459323009699 26.194918882213138 19.67106331468387 78-79 20.265293313577263 33.41880804029411 26.756874316379196 19.559024329749423 80-81 19.648171074263633 33.427531369200935 26.91358216084093 20.0107153956945 82-83 19.590858848277794 32.95568237447548 26.904751180836083 20.548707596410644 84-85 19.659398907315712 32.443461647756386 27.419898346275932 20.477241098651973 86-87 20.000177261192707 32.07932733808898 27.92321709861465 19.997278302103666 88-89 20.48126813816523 32.26775670754382 27.557455909879973 19.693519244410982 90-91 20.871564840371367 31.667141359099503 26.975032777773983 20.48626102275515 92-93 20.685850343550783 31.916709235271018 26.77736226226788 20.620078158910324 94-95 20.300433088452223 31.90881987132994 27.163168237695505 20.627578802522333 96-97 20.616133771838367 32.348702292555586 26.75261458810434 20.282549347501703 98-99 20.991671525973803 31.729863501924065 26.637900306768568 20.640564665333564 100-101 21.145311336599033 31.698231016665556 26.289000962060193 20.86745668467522 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2089.0 1 1308.0 2 397.0 3 211.5 4 161.5 5 175.5 6 225.0 7 315.0 8 406.5 9 531.0 10 708.0 11 845.0 12 843.0 13 771.0 14 708.0 15 611.0 16 524.5 17 494.0 18 515.5 19 563.0 20 662.0 21 802.0 22 1010.0 23 1342.0 24 1823.0 25 2477.0 26 3357.0 27 4625.5 28 6214.5 29 8120.0 30 10539.0 31 13900.0 32 19371.0 33 28227.5 34 41229.5 35 59792.0 36 85199.5 37 116727.0 38 147835.0 39 167476.0 40 173739.5 41 166712.0 42 155756.5 43 152120.5 44 145349.5 45 137936.0 46 138941.0 47 133045.5 48 114629.0 49 96095.5 50 81616.0 51 72403.5 52 65500.0 53 57587.5 54 50018.0 55 42691.0 56 36000.5 57 29977.0 58 24778.0 59 20463.5 60 17016.5 61 14129.0 62 11720.5 63 9693.5 64 7877.5 65 6348.0 66 5080.5 67 4100.5 68 3310.0 69 2617.0 70 2026.0 71 1603.5 72 1285.0 73 1018.0 74 766.5 75 558.0 76 395.5 77 287.0 78 223.0 79 175.5 80 126.5 81 94.5 82 78.5 83 67.5 84 48.5 85 33.5 86 30.5 87 26.5 88 22.0 89 20.0 90 17.5 91 16.0 92 14.5 93 13.0 94 9.0 95 4.5 96 4.0 97 3.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22153462205023633 2 0.23764756865568365 3 0.2199159698798261 4 0.2120066467744125 5 0.22009990762646364 6 0.2432392761534644 7 0.22425690070047175 8 0.2104615697026573 9 0.20332478513312127 10-11 0.18936391016333304 12-13 0.21581415812980928 14-15 0.21224576584504126 16-17 0.21471053164998413 18-19 0.2034351477811038 20-21 0.19622478811291275 22-23 0.16491858363520584 24-25 0.4988943502049618 26-27 0.348322910807483 28-29 0.4838114549806847 30-31 0.3142208525808857 32-33 0.3636081375530615 34-35 0.36419673834230154 36-37 0.37194051747574136 38-39 0.3650980333008254 40-41 0.3703770466293224 42-43 0.4256871270432265 44-45 0.37920605846792366 46-47 0.39693765724378116 48-49 0.39469361673480335 50-51 0.40935345514181415 52-53 0.4143749556250186 54-55 0.37834155105872724 56-57 0.3919897318592317 58-59 0.3896169349276076 60-61 0.3490586617940331 62-63 0.3121055684945541 64-65 0.33776488415048905 66-67 0.34889311782205934 68-69 0.3399169557861481 70-71 0.3106524602961177 72-73 0.30003925231513245 74-75 0.3116273303532966 76-77 0.31898484021879764 78-79 0.3286047843679402 80-81 0.33568638761348496 82-83 0.35452161286916767 84-85 0.3823146063860978 86-87 0.3841539838524731 88-89 0.3781024319880985 90-91 0.35093482680973587 92-93 0.3295796544251191 94-95 0.3074151559552972 96-97 0.3238040091807008 98-99 0.3528477793747662 100-101 0.3701011400093661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.63854306924959 #Duplication Level Percentage of deduplicated Percentage of total 1 91.91981431924273 55.73883619514825 2 4.013709782419914 4.867710270174768 3 1.2826119584653615 2.333271614536091 4 0.5752215875181405 1.3952239603632353 5 0.34184323773807695 1.0364437947256053 6 0.2917358708940438 1.061426290323211 7 0.16513731878747823 0.7009580492344429 8 0.1809069507380222 0.8775947139083338 9 0.13529246364840977 0.7383544095500082 >10 0.8870023826945841 10.813326456010728 >50 0.1017231207987301 4.251857883415166 >100 0.09669885639398777 11.691392091977956 >500 0.006131381532838277 2.407183199818162 >1k 0.0021098079758156306 1.8381207555457346 >5k 6.09611518746595E-5 0.24830031526837004 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 6717 0.24710196883285246 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2761 0.10157042369324186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005003106708540708 0.0 0.0 2 0.00544455730047077 0.0 0.0 3 0.0062538833856758845 0.0 0.0 4 0.006658546428278442 0.0 0.0 5 0.007982898204068629 0.0 0.0 6 0.009344037529186323 0.0 0.0 7 0.011955953531439191 0.0 0.0 8 0.014052843843106988 0.0 0.0 9 0.01574507111217223 0.0 0.0 10-11 0.026431854191812487 0.0 0.0 12-13 0.03594143569297259 0.0 0.0 14-15 0.04603961798337276 0.0 0.0 16-17 0.05429842280739768 0.0 0.0 18-19 0.0632929786179727 0.0 0.0 20-21 0.07024582544087118 0.0 0.0 22-23 0.07686758431982213 0.0 0.0 24-25 0.08426188173465067 0.0 0.0 26-27 0.09167457292414297 0.0 0.0 28-29 0.10201187428517193 0.0 0.0 30-31 0.2045203804862652 0.0 0.0 32-33 0.21818695506143337 0.0 0.0 34-35 0.22824834980250602 0.0 0.0 36-37 0.2395973087700414 0.0 0.0 38-39 0.25359497128915715 0.0 3.67875493275052E-5 40-41 0.26906413578137306 0.0 3.67875493275052E-5 42-43 0.28615195244399927 0.0 3.67875493275052E-5 44-45 0.30772785012458104 0.0 3.67875493275052E-5 46-47 0.3306648871302805 0.0 3.67875493275052E-5 48-49 0.35540451405302775 0.0 3.67875493275052E-5 50-51 0.3841539838524731 0.0 7.35750986550104E-5 52-53 0.41614075799273886 0.0 7.35750986550104E-5 54-55 0.4514200177978164 0.0 7.35750986550104E-5 56-57 0.48955031267577553 0.0 7.35750986550104E-5 58-59 0.5322606574450091 0.0 7.35750986550104E-5 60-61 0.5788336948936306 0.0 1.1036264798251561E-4 62-63 0.6279266794711864 0.0 1.471501973100208E-4 64-65 0.679098160585746 0.0 1.471501973100208E-4 66-67 0.7354750799301477 0.0 1.6554397197377342E-4 68-69 0.7955491479819639 0.0 1.8393774663752601E-4 70-71 0.8587685515012815 0.0 1.8393774663752601E-4 72-73 0.9243791457268871 0.0 1.8393774663752601E-4 74-75 0.9953239346049807 0.0 1.8393774663752601E-4 76-77 1.0711798613182966 0.0 2.2072529596503122E-4 78-79 1.1566189446314274 0.0 2.5751284529253643E-4 80-81 1.2431248668750559 0.0 2.5751284529253643E-4 82-83 1.3428007317779311 0.0 2.5751284529253643E-4 84-85 1.4487856613904737 0.0 2.5751284529253643E-4 86-87 1.560067998106177 0.0 2.5751284529253643E-4 88-89 1.6792780517019574 0.0 2.5751284529253643E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2225 0.0 85.44465 9 CGGGAGA 3145 0.0 82.32934 4 AGAGCGG 2500 0.0 80.59859 8 GAGAGCG 2960 0.0 80.41083 7 ATTCGGA 670 0.0 78.5682 2 ATCGGGA 3920 0.0 77.66899 2 TTCGGAG 605 0.0 77.600136 3 AAGCGGC 675 0.0 77.26083 9 TCGGAGA 930 0.0 76.997505 4 TCGGGAG 3440 0.0 76.647675 3 CGGAAAG 1480 0.0 76.58061 5 GTTCGGA 490 0.0 75.491356 2 GATCCGG 780 0.0 69.92523 1 GGACCGG 605 0.0 69.769455 1 GATCGGG 4625 0.0 68.19313 1 GGATCGG 1595 0.0 68.09347 1 ACCGGAA 435 0.0 66.500244 3 CGGAGAG 1775 0.0 65.45613 5 TCGGGAA 1185 0.0 64.03011 3 ATCCGGA 835 0.0 63.610703 2 >>END_MODULE