FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718311
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT67170.24710196883285246Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC27610.10157042369324186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC22250.085.444659
CGGGAGA31450.082.329344
AGAGCGG25000.080.598598
GAGAGCG29600.080.410837
ATTCGGA6700.078.56822
ATCGGGA39200.077.668992
TTCGGAG6050.077.6001363
AAGCGGC6750.077.260839
TCGGAGA9300.076.9975054
TCGGGAG34400.076.6476753
CGGAAAG14800.076.580615
GTTCGGA4900.075.4913562
GATCCGG7800.069.925231
GGACCGG6050.069.7694551
GATCGGG46250.068.193131
GGATCGG15950.068.093471
ACCGGAA4350.066.5002443
CGGAGAG17750.065.456135
TCGGGAA11850.064.030113
ATCCGGA8350.063.6107032
Produced by FastQC (version 0.11.2)