##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2294152 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.997942159019978 33.0 33.0 33.0 27.0 33.0 2 30.58590755974321 33.0 33.0 33.0 27.0 33.0 3 30.433187077403765 33.0 33.0 33.0 27.0 33.0 4 33.88258580948429 37.0 37.0 37.0 27.0 37.0 5 33.92974659046131 37.0 37.0 37.0 27.0 37.0 6 34.33765461050532 37.0 37.0 37.0 27.0 37.0 7 33.91998873657892 37.0 37.0 37.0 27.0 37.0 8 34.04028983258302 37.0 37.0 37.0 27.0 37.0 9 33.58207259152837 37.0 37.0 37.0 22.0 37.0 10-11 33.586909019105974 37.0 37.0 37.0 18.5 37.0 12-13 33.605415857362544 37.0 37.0 37.0 22.0 37.0 14-15 35.133493334356224 40.0 37.0 40.0 22.0 40.0 16-17 35.03081726930037 40.0 37.0 40.0 18.5 40.0 18-19 35.064776222325285 40.0 37.0 40.0 18.5 40.0 20-21 35.03661919524077 40.0 37.0 40.0 18.5 40.0 22-23 35.64498799556438 38.5 37.0 40.0 27.0 40.0 24-25 35.09892936475003 37.0 37.0 40.0 22.0 40.0 26-27 34.88724809864385 37.0 37.0 40.0 18.5 40.0 28-29 34.61471493606352 37.0 37.0 40.0 15.0 40.0 30-31 34.6955092775021 37.0 37.0 40.0 18.5 40.0 32-33 34.51023493648198 37.0 37.0 40.0 15.0 40.0 34-35 34.4986387126921 37.0 37.0 40.0 15.0 40.0 36-37 34.35918021125017 37.0 37.0 40.0 15.0 40.0 38-39 34.47500231022181 37.0 37.0 40.0 18.5 40.0 40-41 34.174938713738236 37.0 33.0 40.0 15.0 40.0 42-43 34.01234508437105 37.0 33.0 40.0 15.0 40.0 44-45 33.920135195924246 37.0 33.0 40.0 15.0 40.0 46-47 33.837783852159745 37.0 33.0 40.0 15.0 40.0 48-49 33.72109302260704 37.0 33.0 40.0 15.0 40.0 50-51 32.70231506020525 37.0 33.0 38.5 15.0 38.5 52-53 33.16970954845189 37.0 33.0 38.5 15.0 40.0 54-55 33.871882072329996 37.0 33.0 40.0 15.0 40.0 56-57 34.06859811381286 37.0 33.0 40.0 18.5 40.0 58-59 33.624184230164346 37.0 33.0 40.0 15.0 40.0 60-61 33.38835526155198 37.0 33.0 40.0 15.0 40.0 62-63 33.055766139296786 37.0 33.0 40.0 15.0 40.0 64-65 32.81113827680119 37.0 33.0 37.0 15.0 40.0 66-67 32.64472515334642 37.0 33.0 37.0 15.0 40.0 68-69 32.648030732052625 37.0 33.0 37.0 15.0 40.0 70-71 32.2851694220784 37.0 33.0 37.0 15.0 38.5 72-73 32.39282815611171 37.0 33.0 37.0 18.5 37.0 74-75 32.39810243610711 37.0 33.0 37.0 22.0 37.0 76-77 32.1245597501822 37.0 33.0 37.0 22.0 37.0 78-79 31.71723408039223 35.0 33.0 37.0 18.5 37.0 80-81 31.292100959308712 33.0 33.0 37.0 15.0 37.0 82-83 30.989393466518347 33.0 33.0 37.0 15.0 37.0 84-85 30.789119247547678 33.0 33.0 37.0 15.0 37.0 86-87 30.57529884680701 33.0 27.0 37.0 15.0 37.0 88-89 30.466024919011467 33.0 27.0 37.0 15.0 37.0 90-91 30.355157374053682 33.0 27.0 37.0 15.0 37.0 92-93 30.164140606202203 33.0 27.0 37.0 15.0 37.0 94-95 30.02679356032207 33.0 27.0 37.0 15.0 37.0 96-97 29.84890299335005 33.0 27.0 37.0 15.0 37.0 98-99 29.707677390164207 33.0 27.0 37.0 15.0 37.0 100-101 28.45786068229132 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.2639958605444228 2306 2 -0.3499659136672726 2306 3 -0.3833165783974728 2306 4 -0.29238340260075546 2306 5 -0.4011682887615038 2306 6 -0.407040964155847 2306 7 -0.2353863817279631 2306 8 -0.5575177332122507 2306 9 -0.36701406659471303 2306 10-11 -0.28592925972305494 2306 12-13 -0.3822192588315403 2306 14-15 -0.14145499564783393 2306 16-17 -0.12752968541210663 2306 18-19 -0.08485055101596117 2306 20-21 -0.08499045333688571 2306 22-23 -0.22186102446264755 2306 24-25 -0.1616991807679966 2306 26-27 -0.4751988665332547 2306 28-29 -0.3300847454343412 2306 30-31 -0.13084880304069912 2306 32-33 -0.08976877652218462 2306 34-35 -0.24195896543346151 2306 36-37 -0.14056352200272926 2306 38-39 -0.24126909602996705 2306 40-41 -0.17425846831031322 2306 42-43 -0.2627313030855376 2306 44-45 -0.10303994977376618 2306 46-47 -0.1642729538729455 2306 48-49 -0.13813997769962327 2306 50-51 -0.22570343585382346 2306 52-53 -0.17753224752880925 2306 54-55 0.022643822955615178 2306 56-57 -0.08984446134716961 2306 58-59 -0.012442106401401531 2306 60-61 -0.06415431794639659 2306 62-63 0.015735383071067588 2306 64-65 -0.132780327475281 2306 66-67 -0.19082545994008626 2306 68-69 -0.25196436329235183 2306 70-71 -0.1617134791751269 2306 72-73 -0.23085424046023206 2306 74-75 -0.14327060525395297 2306 76-77 -0.09297387813889912 2306 78-79 -0.10767822378910097 2306 80-81 -0.07558923564614517 2306 82-83 -0.13244799142691122 2306 84-85 -0.260681716417416 2306 86-87 -0.251046009462339 2306 88-89 -0.3018904444767294 2306 90-91 -0.27352157787953146 2306 92-93 -0.28347820853801764 2306 94-95 -0.5526959097639441 2306 96-97 -0.35623465099738283 2306 98-99 -0.20553596890184878 2306 100-101 -0.3314035800479367 2307 1 0.048343923635211894 2307 2 0.10085850011245867 2307 3 -0.010537711407483386 2307 4 0.058379410756217 2307 5 0.05424488747511447 2307 6 -0.0760769019050187 2307 7 0.4082983241691025 2307 8 -0.09572062686865479 2307 9 0.0745268195132951 2307 10-11 0.07255862187905393 2307 12-13 0.1479688569772577 2307 14-15 0.27095811492335997 2307 16-17 0.3366557662565768 2307 18-19 0.2727581956186924 2307 20-21 0.21344577146400923 2307 22-23 0.09445057986877003 2307 24-25 0.2998224434819363 2307 26-27 -0.08489639907671886 2307 28-29 0.09284937338255617 2307 30-31 0.2313745031821952 2307 32-33 0.37139872346999425 2307 34-35 0.22202417418686338 2307 36-37 0.3472997875972439 2307 38-39 0.07741490630897374 2307 40-41 0.2072198167589363 2307 42-43 0.26161556045232714 2307 44-45 0.045979915580836916 2307 46-47 0.25251816440924557 2307 48-49 0.14729214770257926 2307 50-51 0.13531475346898247 2307 52-53 -0.017754822051252006 2307 54-55 0.12297543335326822 2307 56-57 0.18729662238250455 2307 58-59 0.3281546046988737 2307 60-61 0.3357157583193029 2307 62-63 0.40980063574224346 2307 64-65 0.3474048177547431 2307 66-67 0.1704952593777449 2307 68-69 0.0051684653552896975 2307 70-71 0.19200693620558695 2307 72-73 -0.11067025219245608 2307 74-75 -0.057750144220129584 2307 76-77 3.3966175909938556E-4 2307 78-79 0.13550132737208642 2307 80-81 0.15719610407862206 2307 82-83 0.14887347835292175 2307 84-85 0.2790805747430838 2307 86-87 0.1398594854829902 2307 88-89 0.3224484454023546 2307 90-91 0.24574715943687764 2307 92-93 0.21958555258992263 2307 94-95 0.09525032588737758 2307 96-97 0.049709789798981774 2307 98-99 -0.08724048699579967 2307 100-101 -0.19664796487234426 2201 1 0.13682004397451664 2201 2 0.02378093589670982 2201 3 0.06320334690887464 2201 4 0.16416941559402431 2201 5 0.03384858947210745 2201 6 0.09893074240007849 2201 7 0.22491797656496715 2201 8 -0.05076407379708314 2201 9 0.179971718070135 2201 10-11 0.14950698657197847 2201 12-13 0.07888228324811308 2201 14-15 0.23695236985123813 2201 16-17 0.34610010781992173 2201 18-19 0.3509247682568173 2201 20-21 0.3938841023784434 2201 22-23 0.16492231603761098 2201 24-25 0.10326187833451428 2201 26-27 -0.07339132512435498 2201 28-29 0.10556562108389045 2201 30-31 0.42179105679927886 2201 32-33 0.2789126806073341 2201 34-35 0.38439237272920934 2201 36-37 0.2356159876383046 2201 38-39 0.23308468381303982 2201 40-41 0.2677675940246971 2201 42-43 0.15813707863286197 2201 44-45 0.14402225570001548 2201 46-47 0.17488475513530233 2201 48-49 0.20599868821308576 2201 50-51 0.24760162808945552 2201 52-53 0.3605317625880744 2201 54-55 0.5016471761256298 2201 56-57 0.5425627231552568 2201 58-59 0.709737643385516 2201 60-61 0.7401804631629929 2201 62-63 0.6084513620588936 2201 64-65 0.46806482633933655 2201 66-67 0.4714263572722217 2201 68-69 0.3592549744846636 2201 70-71 0.3754560466668764 2201 72-73 0.3362505642911131 2201 74-75 0.24637792408909576 2201 76-77 0.4720330511937405 2201 78-79 0.38891226655212563 2201 80-81 0.5187734579862635 2201 82-83 0.4014774947354205 2201 84-85 0.5830749933236952 2201 86-87 0.22796207564631032 2201 88-89 0.3601484354970559 2201 90-91 0.5194250535420792 2201 92-93 0.32414500375034905 2201 94-95 0.17247560822422514 2201 96-97 0.24665795351487674 2201 98-99 0.3839602215918063 2201 100-101 0.18531391552621912 1101 1 -0.13292494854275105 1101 2 -0.2697807341631382 1101 3 -0.3286194294892013 1101 4 -0.15230234538007892 1101 5 -0.3173705295948466 1101 6 -0.2406373301018121 1101 7 0.12228172906159784 1101 8 -0.2922791688817412 1101 9 -0.08078799943788795 1101 10-11 -0.18232849664443052 1101 12-13 -0.19507666788645395 1101 14-15 -0.15310313641062123 1101 16-17 -0.26135505448151264 1101 18-19 -0.2803655893232815 1101 20-21 -0.24868081793530195 1101 22-23 -0.25195326198961254 1101 24-25 -0.2828470028238783 1101 26-27 -0.2516577885653888 1101 28-29 -0.20027474246882804 1101 30-31 0.0684611766325034 1101 32-33 0.19289584042165586 1101 34-35 -0.10504338924433654 1101 36-37 -0.0929086026111392 1101 38-39 -0.2787867498926886 1101 40-41 -0.17508562918287396 1101 42-43 -0.23153750997346378 1101 44-45 -0.23892896023712495 1101 46-47 -0.2166195683648482 1101 48-49 -0.20167996490589246 1101 50-51 -0.15856052676993926 1101 52-53 -0.05675994287454955 1101 54-55 0.044458486654498586 1101 56-57 0.11754794808837232 1101 58-59 0.19624955915746511 1101 60-61 0.07415709221518085 1101 62-63 0.08709363121705138 1101 64-65 0.07001213581661148 1101 66-67 -0.02863858521064344 1101 68-69 -0.06468485904349563 1101 70-71 -0.09331392184254383 1101 72-73 -0.3003897237301061 1101 74-75 -0.18032809407080208 1101 76-77 -0.053392582882239026 1101 78-79 0.11371797377054804 1101 80-81 0.09644857969473719 1101 82-83 -0.17238662472536603 1101 84-85 -0.24280742896310414 1101 86-87 -0.1591337226011298 1101 88-89 -0.2670695850247675 1101 90-91 -0.2758062121164926 1101 92-93 -0.10000387154495272 1101 94-95 -0.036606324151090774 1101 96-97 -0.045846510302773424 1101 98-99 -0.12053411119851276 1101 100-101 -0.3370156052399871 2304 1 -0.019539620768934896 2304 2 0.08038165844529743 2304 3 -0.041669169877071255 2304 4 0.09694690386916704 2304 5 0.07929592102843941 2304 6 -0.1238046701036879 2304 7 0.3545773340190763 2304 8 -4.276050877365378E-4 2304 9 0.21341339620235544 2304 10-11 0.10224528577108316 2304 12-13 0.059051561061920665 2304 14-15 0.20770227331565394 2304 16-17 0.11861664383550874 2304 18-19 0.1689509934465434 2304 20-21 0.07449775632240829 2304 22-23 -0.014987159196174105 2304 24-25 -0.002930395569137545 2304 26-27 -0.17869309725536908 2304 28-29 0.016275260771827504 2304 30-31 0.15888109295170239 2304 32-33 0.2983642584800066 2304 34-35 0.2730656550517594 2304 36-37 0.2914026870958324 2304 38-39 0.042947934431929013 2304 40-41 0.3531044313812828 2304 42-43 0.27716939837372934 2304 44-45 0.2991558872002358 2304 46-47 0.23947537549987885 2304 48-49 0.1845436687997477 2304 50-51 0.2548323625222366 2304 52-53 0.19361129166934177 2304 54-55 0.27869020038261993 2304 56-57 0.1911751724350239 2304 58-59 0.3596507301125911 2304 60-61 0.3083974476785656 2304 62-63 0.31538955303815897 2304 64-65 0.38409348335120086 2304 66-67 0.4282910062513139 2304 68-69 0.3166554740953984 2304 70-71 0.3069741359994751 2304 72-73 0.04606320401778419 2304 74-75 0.12933192566115537 2304 76-77 0.10189147815722777 2304 78-79 0.1001702533882991 2304 80-81 0.16451520747359183 2304 82-83 0.33161521884482426 2304 84-85 0.12258098102962123 2304 86-87 0.16603821192332546 2304 88-89 0.25733359513380805 2304 90-91 0.07814306089752776 2304 92-93 0.10057332435598454 2304 94-95 0.29216849383236365 2304 96-97 0.2610441933505214 2304 98-99 0.1905921229493046 2304 100-101 0.015669453919894494 2202 1 -0.05822304960403457 2202 2 -0.1706200563715612 2202 3 -0.14657336862723014 2202 4 -0.11905679640835132 2202 5 -0.0697755710840724 2202 6 -0.21003688692042743 2202 7 0.16740363172282002 2202 8 -0.303588813401376 2202 9 -0.05532081162102287 2202 10-11 -0.20597008457833965 2202 12-13 -0.24735049583095758 2202 14-15 -0.11040479514613821 2202 16-17 -0.03232051578000039 2202 18-19 -0.10943077847171878 2202 20-21 -0.13564062695802193 2202 22-23 -0.21589847454722388 2202 24-25 -0.39990717122539365 2202 26-27 -0.263749899177391 2202 28-29 -0.12048959191587016 2202 30-31 -0.07048697596042075 2202 32-33 -0.03728762704210453 2202 34-35 -0.053478179260991965 2202 36-37 0.09021740035759507 2202 38-39 -0.030169260988422764 2202 40-41 0.022896812092810137 2202 42-43 -0.10225512860967712 2202 44-45 -0.09995997274825186 2202 46-47 0.03174882778338173 2202 48-49 -0.1302007613730325 2202 50-51 -0.18022488307813944 2202 52-53 -0.12311538249548448 2202 54-55 -0.15189975873241934 2202 56-57 0.0878903996981677 2202 58-59 0.13313163086839808 2202 60-61 0.08076893069566182 2202 62-63 0.07131698500563033 2202 64-65 0.07547736052327991 2202 66-67 0.03489881898354952 2202 68-69 0.012914710924256667 2202 70-71 0.1441923170512993 2202 72-73 -0.1485049527426483 2202 74-75 0.011066353105327664 2202 76-77 0.00290284297841481 2202 78-79 -0.03362594739390801 2202 80-81 -0.059015163419331884 2202 82-83 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82-83 -0.4014567565777156 1213 84-85 -0.3348918382844843 1213 86-87 -0.2687578974635514 1213 88-89 -0.24981816347209929 1213 90-91 -0.20879732603181722 1213 92-93 -0.2992815790573893 1213 94-95 -0.39461383598015587 1213 96-97 -0.29817246913656703 1213 98-99 -0.3637797553076041 1213 100-101 -0.1487028921431346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2388.0 3 265.0 4 368.0 5 227.0 6 559.0 7 3167.0 8 19564.0 9 51100.0 10 53170.0 11 30462.0 12 16923.0 13 10775.0 14 7871.0 15 6650.0 16 6374.0 17 6343.0 18 6787.0 19 7439.0 20 8184.0 21 9124.0 22 9823.0 23 11243.0 24 12782.0 25 15158.0 26 17485.0 27 21356.0 28 26219.0 29 31944.0 30 39606.0 31 51778.0 32 70490.0 33 100600.0 34 152407.0 35 253333.0 36 463995.0 37 637458.0 38 130628.0 39 107.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.2204910985762 23.49829974767067 22.2751840046971 14.006025149056036 2 32.90592464813717 24.515178207837195 29.969554449821644 12.609342694203985 3 36.62368615445638 20.300046698005442 27.353061820907687 15.723205326630488 4 30.125839926581367 17.810309272946707 32.737862534000016 19.325988266471906 5 33.806269262441 16.08733544332829 32.168380218469665 17.93801507576105 6 31.20072220212221 16.4988315776411 36.112247198670254 16.188199021566437 7 36.585376508872926 17.863753542419257 27.918828960399605 17.632040988308216 8 29.941826435032386 29.546518758987244 23.194907214081166 17.316747591899208 9 31.282987321337785 27.087122343761738 23.423160178832557 18.20673015606792 10-11 26.67079942982717 29.099842259170106 27.609252556519433 16.620105754483298 12-13 28.619797553562908 24.555274167603113 27.432202066399547 19.392726212434436 14-15 26.31235132086911 24.709521492351897 28.79321250009828 20.184914686680706 16-17 27.101008686854968 25.51914622322856 29.59340416688599 17.786440923030487 18-19 26.716955436544186 25.607810053721092 29.70560737764688 17.969627132087844 20-21 25.393255188190793 27.481480963859 28.958527229457857 18.16673661849235 22-23 27.759128620967804 25.49458226046409 28.408951740804252 18.337337377763856 24-25 25.028129578517387 27.81640217817232 28.59036258846005 18.565105654850235 26-27 22.515636998125025 30.080337613500692 28.917647877044715 18.486377511329565 28-29 23.37114607245522 29.207395406144208 28.31825072390831 19.10320779749226 30-31 25.63607786653444 27.365160237511336 28.012371857980305 18.986390037973916 32-33 23.412301645024296 26.97675792080128 29.468563422735482 20.14237701143894 34-35 20.780613329670167 27.014989819836927 33.31634452086736 18.888052329625545 36-37 21.341359638895003 27.726039539719753 30.595353358846122 20.337247462539118 38-39 20.529955166995066 27.36213238510886 28.13172626318129 23.97618618471478 40-41 21.86351952924191 26.610497992934253 29.12266617082481 22.403316306999027 42-43 24.023130142068275 26.707194025734694 30.293088316082763 18.976587516114275 44-45 22.304817830850542 28.248398216669923 30.805607264407627 18.641176688071905 46-47 23.51349772359321 28.934643373147566 29.049825815002205 18.502033088257015 48-49 21.045064292225373 29.092084286422597 30.26016656506702 19.60268485628501 50-51 19.894017097757903 27.727101138938703 31.00933231646937 21.36954944683403 52-53 23.92423188965289 26.974744619764284 28.47808326893705 20.622940221645774 54-55 26.085421304658297 26.88630619845704 28.102138201649375 18.926134295235293 56-57 26.651699140917117 26.818338858452297 27.159123207271936 19.370838793358654 58-59 24.97275450877052 26.79030937881593 27.765961613230644 20.47097449918291 60-61 21.604691348898406 27.13191586523373 27.499496427533355 23.763896358334513 62-63 23.434330626031635 27.243974158568445 27.566095692001618 21.7555995233983 64-65 21.81923945774899 28.2611491015332 29.421512905687774 20.49809853503004 66-67 20.92648654748829 28.147591171719476 28.7066963584677 22.219225922324533 68-69 20.946079818541342 27.011307488187562 32.39392204468148 19.648690648589614 70-71 20.380240456330522 30.61457748740559 30.154853911837055 18.850328144426836 72-73 20.114708099212866 33.98489212533293 27.059320136895987 18.84107963855822 74-75 20.822100495953773 33.564647251922 26.526498054772446 19.08675419735178 76-77 20.86072262919749 32.88370613385399 26.77029504461909 19.485276192329433 78-79 20.774606156737878 32.594670854483425 27.316582940944173 19.314140047834524 80-81 19.819170878402847 32.70992640176902 27.528069494084328 19.94283322574381 82-83 19.245351604457316 32.25182621202322 27.947064073965773 20.555758109553693 84-85 19.407222855222603 31.64503818129948 28.217341118362903 20.730397845115018 86-87 19.357716051476004 31.699845386458374 28.97232861733225 19.970109944733373 88-89 19.825758535361857 31.775626103010428 28.75345136843846 19.645163993189254 90-91 20.508564582229745 31.08161013008612 27.946096869735854 20.46372841794829 92-93 19.961064402923732 31.78221430847113 27.617880985998184 20.638840302606955 94-95 19.761676462910906 31.804170432350826 27.86827079346299 20.565882311275278 96-97 20.27267453822676 32.38984228965449 27.288247475517167 20.049235696601585 98-99 20.64515860929231 31.520771313783218 27.18182508130207 20.652244995622407 100-101 20.72592674582895 31.563946955158457 26.75381328431026 20.956313014702328 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1755.0 1 1090.0 2 323.5 3 193.0 4 156.0 5 153.0 6 202.5 7 299.5 8 406.0 9 514.5 10 621.5 11 711.0 12 728.0 13 671.5 14 594.5 15 521.5 16 457.5 17 436.0 18 472.5 19 508.5 20 546.5 21 612.0 22 734.5 23 896.0 24 1088.0 25 1386.0 26 1843.0 27 2543.5 28 3385.5 29 4201.5 30 5253.5 31 6889.5 32 10917.0 33 19176.5 34 33173.0 35 53046.0 36 80339.0 37 114163.5 38 143048.0 39 158505.5 40 161641.5 41 149627.5 42 138994.5 43 141298.5 44 138055.5 45 128516.0 46 123390.0 47 111436.0 48 90936.0 49 76219.5 50 66184.5 51 56799.5 52 47789.5 53 41003.5 54 34503.5 55 27154.0 56 21756.0 57 18051.0 58 14865.0 59 11992.5 60 9559.0 61 7495.5 62 5851.5 63 4573.5 64 3557.0 65 2719.0 66 2015.0 67 1573.5 68 1286.5 69 966.5 70 685.0 71 506.0 72 345.5 73 250.0 74 195.5 75 139.5 76 106.5 77 82.0 78 64.5 79 50.0 80 38.0 81 28.5 82 25.5 83 21.5 84 14.5 85 14.0 86 14.5 87 11.0 88 10.5 89 10.5 90 10.5 91 10.0 92 6.5 93 3.0 94 2.0 95 3.0 96 2.5 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22143258162493157 2 0.23869386160986716 3 0.2168557270834714 4 0.20988147254410344 5 0.2199941416261869 6 0.2439245525143931 7 0.222696665260192 8 0.2090532798175535 9 0.2020790252781856 10-11 0.18830487256293393 12-13 0.2137826961770624 14-15 0.210360952543685 16-17 0.21308527072312558 18-19 0.20231876527797635 20-21 0.1953227161931729 22-23 0.1638731871297107 24-25 0.49746049956585264 26-27 0.34816786333250804 28-29 0.48205175594293664 30-31 0.31329659063566845 32-33 0.3626394415016965 34-35 0.3632714833193267 36-37 0.37055086149479194 38-39 0.3633586615010688 40-41 0.3695047233138868 42-43 0.42224752326785675 44-45 0.377154608761756 46-47 0.39585432874543625 48-49 0.3940017923834166 50-51 0.40753620509887745 52-53 0.4128104850942745 54-55 0.3783533087607099 56-57 0.39153900874920233 58-59 0.3884441832973578 60-61 0.348124274241637 62-63 0.31065945063796996 64-65 0.3381205778867311 66-67 0.3486691378775251 68-69 0.3396026069763468 70-71 0.31059406700166337 72-73 0.2993698761023681 74-75 0.3114222597282133 76-77 0.3179606233588707 78-79 0.3277463742594214 80-81 0.3349385742531445 82-83 0.351654990602192 84-85 0.3809686542129728 86-87 0.3825596560297661 88-89 0.37684948512565863 90-91 0.3509357706028197 92-93 0.32933737607621466 94-95 0.3073466797317702 96-97 0.32240671062771775 98-99 0.35213447060177355 100-101 0.36725988513402774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2294152.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.83826729800237 #Duplication Level Percentage of deduplicated Percentage of total 1 88.12509332710657 38.63251396935103 2 4.889259972118669 4.286733710943237 3 1.8687111187462535 2.457631725790419 4 1.1123113918004022 1.950472164494363 5 0.6774543176168624 1.4849211728936904 6 0.4617001442061181 1.2144080599940428 7 0.32152098771358834 0.9866446100914217 8 0.2850310972521073 0.9996215543664632 9 0.20365570325947654 0.8035121840626429 >10 1.579646188076927 13.889038434566995 >50 0.23932040616054906 7.392722396702989 >100 0.2192431682113211 19.194035187920925 >500 0.0129274743857628 3.6873395773474695 >1k 0.004124703345403549 3.0204052514743194 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 3928 0.1712179489414825 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3504 0.15273617441215753 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3188 0.13896202169690586 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3153 0.13743640351641914 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2755 0.12008794534974143 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2666 0.11620851626221802 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2588 0.11280856717427618 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2580 0.11245985444730776 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2460 0.10722916354278182 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004707621814073348 0.0 0.0 2 0.005056334541041744 0.0 0.0 3 0.006015294540204834 0.0 0.0 4 0.006494774539786379 0.0 0.0 5 0.0076280909024336666 0.0 0.0 6 0.008935763628565152 0.0 0.0 7 0.01076650544514923 0.0 0.0 8 0.012422890898249113 0.0 0.0 9 0.014210043623962144 0.0 0.0 10-11 0.020726612709184047 0.0 0.0 12-13 0.02791881270290722 0.0 0.0 14-15 0.0345879436061778 0.0 0.0 16-17 0.039971196328752415 0.0 0.0 18-19 0.046509559959409835 0.0 0.0 20-21 0.05298253995376069 0.0 0.0 22-23 0.058867067221352376 0.0 0.0 24-25 0.06564517085180058 0.0 0.0 26-27 0.07307711084531451 0.0 0.0 28-29 0.08179492901952443 0.0 0.0 30-31 0.14009533805955315 0.0 0.0 32-33 0.15068748714121818 0.0 0.0 34-35 0.1602117034965425 0.0 0.0 36-37 0.17141409985040224 0.0 0.0 38-39 0.18479595074781444 0.0 0.0 40-41 0.20031366709790807 0.0 0.0 42-43 0.21842493435482915 0.0 0.0 44-45 0.23740798342917122 0.0 0.0 46-47 0.25776408886595137 0.0 0.0 48-49 0.28121501975457597 0.0 0.0 50-51 0.30621336336912286 0.0 0.0 52-53 0.3357013833433879 0.0 0.0 54-55 0.3675650087701251 0.0 0.0 56-57 0.402283719648916 0.0 0.0 58-59 0.4390511177986463 0.0 0.0 60-61 0.47934923230893156 0.0 2.179454543552476E-5 62-63 0.5203011831822826 0.0 8.717818174209904E-5 64-65 0.5632146431448308 0.0 8.717818174209904E-5 66-67 0.6106177794670973 0.0 8.717818174209904E-5 68-69 0.6638182648752131 0.0 8.717818174209904E-5 70-71 0.716975161192458 0.0 8.717818174209904E-5 72-73 0.77457814477855 0.0 8.717818174209904E-5 74-75 0.8355592829071483 0.0 8.717818174209904E-5 76-77 0.8981314228525399 0.0 8.717818174209904E-5 78-79 0.9672637209740244 0.0 8.717818174209904E-5 80-81 1.0445035899975241 0.0 8.717818174209904E-5 82-83 1.1273010681070827 0.0 8.717818174209904E-5 84-85 1.2202765989350315 0.0 8.717818174209904E-5 86-87 1.3176110388500848 0.0 8.717818174209904E-5 88-89 1.4165364805819318 0.0 8.717818174209904E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGG 1635 0.0 82.90774 8 GAGCGGC 1580 0.0 82.19222 9 CGGGAGA 2100 0.0 81.94086 4 CGGAAAG 990 0.0 79.48516 5 AACCGGA 290 0.0 78.46489 2 TCGGAGA 640 0.0 77.03118 4 AAGCGGC 660 0.0 76.83819 9 GAGAGCG 2070 0.0 76.24829 7 TCGGGAG 2210 0.0 73.357925 3 GGATCGG 1010 0.0 71.35129 1 ACCGGAA 450 0.0 69.52557 3 GAACCGG 350 0.0 67.73007 1 ATCGGAG 575 0.0 66.77753 3 ATTCGGA 300 0.0 66.368225 2 GATCCGG 380 0.0 66.12594 1 AATCGGA 495 0.0 64.16553 2 GAATCGG 530 0.0 63.512917 1 ATCGGGA 3055 0.0 63.31128 2 GTGCGAC 30 1.3136245E-4 63.21474 1 GTTCGGA 320 0.0 62.22021 2 >>END_MODULE