FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294152
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT39280.1712179489414825Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA35040.15273617441215753No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG31880.13896202169690586No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC31530.13743640351641914No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG27550.12008794534974143No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26660.11620851626221802No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT25880.11280856717427618No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC25800.11245985444730776No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT24600.10722916354278182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG16350.082.907748
GAGCGGC15800.082.192229
CGGGAGA21000.081.940864
CGGAAAG9900.079.485165
AACCGGA2900.078.464892
TCGGAGA6400.077.031184
AAGCGGC6600.076.838199
GAGAGCG20700.076.248297
TCGGGAG22100.073.3579253
GGATCGG10100.071.351291
ACCGGAA4500.069.525573
GAACCGG3500.067.730071
ATCGGAG5750.066.777533
ATTCGGA3000.066.3682252
GATCCGG3800.066.125941
AATCGGA4950.064.165532
GAATCGG5300.063.5129171
ATCGGGA30550.063.311282
GTGCGAC301.3136245E-463.214741
GTTCGGA3200.062.220212
Produced by FastQC (version 0.11.2)