FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256177
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT170150.32371436502233464Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC89030.16938166275602973No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG87050.16561466632497346No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG79880.1519735731882697No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC77970.14833975339871547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGA12100.090.5405962
GAGCGGC51450.090.504119
CGGGAGA66550.089.576824
AGAGCGG55150.087.443178
CGGAGAG27050.086.785345
GAGAGCG61100.086.612737
TTCGGAG11050.086.26653
CGGAAAG31150.085.7153855
GTTCGGA11950.084.136542
GTCCGGA5600.083.842432
AAGCGGC18500.082.789069
TCGGAGA18200.082.603094
GAACCGG8450.081.949441
CCGGAGA12800.081.882444
TCGGGAG73600.080.996433
GGATCGG26200.080.3769451
AAGGGGC53450.077.359029
AACCGGA8650.075.1140442
ACCGGAA8850.073.951033
TTGGGAG37400.073.927393
Produced by FastQC (version 0.11.2)