##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2809291 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.58576131842518 33.0 33.0 33.0 22.0 33.0 2 29.920754382511458 33.0 33.0 33.0 15.0 33.0 3 29.73679479982672 33.0 33.0 33.0 15.0 33.0 4 32.97114218498547 37.0 37.0 37.0 15.0 37.0 5 33.04809576508806 37.0 37.0 37.0 15.0 37.0 6 33.739148062625056 37.0 37.0 37.0 22.0 37.0 7 33.112616314934975 37.0 37.0 37.0 15.0 37.0 8 33.40161556777137 37.0 37.0 37.0 22.0 37.0 9 32.68273916799648 37.0 37.0 37.0 15.0 37.0 10-11 32.7672444755634 37.0 37.0 37.0 15.0 37.0 12-13 32.771754688282556 37.0 37.0 37.0 15.0 37.0 14-15 34.304105555458655 40.0 37.0 40.0 15.0 40.0 16-17 34.19504102636573 40.0 37.0 40.0 15.0 40.0 18-19 34.26587615878882 40.0 37.0 40.0 15.0 40.0 20-21 34.18891563743307 40.0 37.0 40.0 15.0 40.0 22-23 35.18107006358544 40.0 37.0 40.0 22.0 40.0 24-25 34.40968219383467 38.5 37.0 40.0 15.0 40.0 26-27 34.223335709970954 37.0 37.0 40.0 10.5 40.0 28-29 33.82130669268509 37.0 37.0 40.0 15.0 40.0 30-31 33.87968067387821 37.0 37.0 40.0 10.5 40.0 32-33 33.78201937784303 37.0 33.0 40.0 10.5 40.0 34-35 33.79266992988622 37.0 33.0 40.0 15.0 40.0 36-37 33.67175792753403 37.0 33.0 40.0 10.5 40.0 38-39 33.5006756153065 37.0 33.0 40.0 6.0 40.0 40-41 33.52432090516788 37.0 33.0 40.0 10.5 40.0 42-43 33.376819097772355 37.0 33.0 40.0 10.5 40.0 44-45 33.20039273254355 37.0 33.0 40.0 6.0 40.0 46-47 33.10073573723761 37.0 33.0 40.0 6.0 40.0 48-49 32.9993774229868 37.0 33.0 40.0 6.0 40.0 50-51 32.126101567975695 37.0 33.0 38.5 15.0 38.5 52-53 32.51751011198199 37.0 33.0 38.5 10.5 40.0 54-55 33.148326570654305 37.0 33.0 40.0 10.5 40.0 56-57 33.508380584282655 37.0 33.0 40.0 15.0 40.0 58-59 32.91700877552379 37.0 33.0 40.0 6.0 40.0 60-61 32.71673618005397 37.0 33.0 40.0 6.0 40.0 62-63 32.340346550072596 37.0 33.0 40.0 6.0 40.0 64-65 32.17202383092389 37.0 33.0 40.0 6.0 40.0 66-67 32.09281096191174 37.0 33.0 37.0 6.0 40.0 68-69 32.247125876244226 37.0 33.0 37.0 15.0 40.0 70-71 31.838039918256953 37.0 33.0 37.0 15.0 40.0 72-73 32.196462381433605 37.0 33.0 37.0 15.0 40.0 74-75 32.394952498690955 37.0 33.0 37.0 22.0 37.0 76-77 32.1157544732817 37.0 33.0 37.0 22.0 37.0 78-79 31.589877303561646 37.0 33.0 37.0 18.5 37.0 80-81 31.00555175665319 37.0 27.0 37.0 15.0 37.0 82-83 30.618900818747505 33.0 27.0 37.0 15.0 37.0 84-85 30.358155313920843 33.0 27.0 37.0 10.5 37.0 86-87 30.110688960310625 33.0 27.0 37.0 6.0 37.0 88-89 29.9738277380307 33.0 27.0 37.0 6.0 37.0 90-91 29.840376628836246 33.0 27.0 37.0 6.0 37.0 92-93 29.664714157415517 33.0 27.0 37.0 6.0 37.0 94-95 29.53342426968228 33.0 27.0 37.0 6.0 37.0 96-97 29.3765450072634 33.0 27.0 37.0 6.0 37.0 98-99 29.248576420171496 33.0 27.0 37.0 6.0 37.0 100-101 28.081745358526405 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.06830862845932018 2306 2 0.06864936996550597 2306 3 -0.08330331072260933 2306 4 0.1864175322937669 2306 5 0.04348034173273163 2306 6 -0.18310975113882932 2306 7 0.5714047220661058 2306 8 -0.22786086007807427 2306 9 0.24225485628615218 2306 10-11 0.181240123292838 2306 12-13 0.076619443463251 2306 14-15 0.5045340979446848 2306 16-17 0.4342019912004389 2306 18-19 0.482849295590988 2306 20-21 0.3516387520560471 2306 22-23 0.14313716517713004 2306 24-25 0.35092857637719277 2306 26-27 -0.03301722073067026 2306 28-29 0.28759371503428355 2306 30-31 0.39758419963002467 2306 32-33 0.49741316774865396 2306 34-35 0.4073665384288745 2306 36-37 0.31515274885677513 2306 38-39 0.3699232324284125 2306 40-41 0.25017932474090543 2306 42-43 0.3979961032460082 2306 44-45 0.38682523084531084 2306 46-47 0.32930878377000994 2306 48-49 0.34914701998941666 2306 50-51 0.33378530981164545 2306 52-53 0.35684237188355183 2306 54-55 0.551323406881032 2306 56-57 0.6374477425952279 2306 58-59 0.7150612408683159 2306 60-61 0.495433042577055 2306 62-63 0.48497753820936396 2306 64-65 0.4470259718258731 2306 66-67 0.4109448676268741 2306 68-69 0.2728638715674023 2306 70-71 0.4238627332975362 2306 72-73 0.019675275595005814 2306 74-75 0.12409269457304362 2306 76-77 0.18127903796304423 2306 78-79 0.32107877864359935 2306 80-81 0.34125723335017355 2306 82-83 0.3303623927344681 2306 84-85 0.34264303111365635 2306 86-87 0.4226528939697367 2306 88-89 0.4095810005116043 2306 90-91 0.2821795235481517 2306 92-93 0.30256009628872604 2306 94-95 0.3575198380581348 2306 96-97 0.14914558242895737 2306 98-99 0.2728512985146061 2306 100-101 0.14382104552067787 2307 1 -0.07682880783151802 2307 2 -0.06409930804088049 2307 3 -0.20168041127107017 2307 4 -0.1033848419511898 2307 5 -0.06139767576845401 2307 6 -0.38303408650840964 2307 7 0.2501378311129443 2307 8 -0.3440582207931726 2307 9 0.032924167652517156 2307 10-11 -0.1668338485017813 2307 12-13 -0.22865506525101154 2307 14-15 0.13408647967060006 2307 16-17 0.014440911889380459 2307 18-19 0.08394878389289318 2307 20-21 0.060280550565835256 2307 22-23 -0.05251382850893549 2307 24-25 0.046534043039869744 2307 26-27 -0.22701812699630608 2307 28-29 -0.18979282008137233 2307 30-31 0.10565509354136537 2307 32-33 0.13728842797942065 2307 34-35 0.08619241145763823 2307 36-37 -0.07373523756634626 2307 38-39 0.001317428053546621 2307 40-41 0.048533939642133817 2307 42-43 0.227908640063049 2307 44-45 0.25237815975048505 2307 46-47 0.17659682662098675 2307 48-49 0.2568539114555577 2307 50-51 0.0036491989736475716 2307 52-53 -0.06526623464753811 2307 54-55 0.10584639909655635 2307 56-57 0.028540262911398884 2307 58-59 0.17540955465850772 2307 60-61 0.16643361113049338 2307 62-63 0.23424226609456156 2307 64-65 0.1081435426919981 2307 66-67 0.08789665173046046 2307 68-69 -0.1330185190929285 2307 70-71 0.027930455279179256 2307 72-73 -0.3349855801420425 2307 74-75 -0.3220310961821866 2307 76-77 -0.22804908576786787 2307 78-79 -0.06528619846464423 2307 80-81 0.07780987584120425 2307 82-83 9.145307946312187E-4 2307 84-85 0.09079074386164976 2307 86-87 0.14093145950513986 2307 88-89 0.10348444047364325 2307 90-91 0.13453260805741962 2307 92-93 0.029470414464906725 2307 94-95 -0.0718194429996295 2307 96-97 -2.95482518655632E-4 2307 98-99 0.06267312327190311 2307 100-101 -0.2538855039584327 2201 1 -0.002886063036754649 2201 2 -0.05757388447509726 2201 3 -0.1539634438231623 2201 4 0.05009804516398475 2201 5 -0.0528028541691512 2201 6 -0.23480328043007148 2201 7 0.2500214431041812 2201 8 -0.2518783407209142 2201 9 0.04705598043904047 2201 10-11 -0.04077005248282717 2201 12-13 -0.101256750857182 2201 14-15 0.16065815063689115 2201 16-17 0.2183980079989425 2201 18-19 0.09792958083333048 2201 20-21 0.11805026775259364 2201 22-23 -0.12328120062159797 2201 24-25 0.010219830044043476 2201 26-27 -0.31371815181292106 2201 28-29 -0.06804201738886206 2201 30-31 0.19248805077791786 2201 32-33 0.34501332135453566 2201 34-35 0.3122101332152738 2201 36-37 0.06680924670011734 2201 38-39 0.12941522476120326 2201 40-41 0.10023416645446304 2201 42-43 0.1979771069925036 2201 44-45 0.07165826625031713 2201 46-47 0.21788532844760056 2201 48-49 0.10804203102932775 2201 50-51 0.04187352195133087 2201 52-53 0.08644726616909537 2201 54-55 0.35573366624839764 2201 56-57 0.2375795712780615 2201 58-59 0.470395732380112 2201 60-61 0.27889405363577424 2201 62-63 0.32440449751239697 2201 64-65 0.33704124244172107 2201 66-67 0.15440383796858725 2201 68-69 0.1296267013998289 2201 70-71 0.18671015105439537 2201 72-73 -0.004172383857998341 2201 74-75 0.13898921304440393 2201 76-77 0.16496792227156476 2201 78-79 0.21944994703383358 2201 80-81 0.3372951138017761 2201 82-83 0.3511678911376812 2201 84-85 0.2594513006388013 2201 86-87 0.22352798941108531 2201 88-89 0.30814121127391303 2201 90-91 0.3587630524672356 2201 92-93 -0.03670828826087913 2201 94-95 0.10571642085910327 2201 96-97 0.13336979711074903 2201 98-99 0.03585463277628165 2201 100-101 0.0019280289851444365 1101 1 0.08486110569200989 1101 2 0.011007755885682258 1101 3 0.07640610188380847 1101 4 0.3932652263679799 1101 5 0.18827906912136427 1101 6 -0.1021047624554825 1101 7 0.45240015836633063 1101 8 0.1060311901947486 1101 9 0.36282503608539685 1101 10-11 0.2284232424773336 1101 12-13 0.18956185039086648 1101 14-15 0.38089565369648426 1101 16-17 0.41255044264217133 1101 18-19 0.3397210655696057 1101 20-21 0.3210153713311996 1101 22-23 0.03321526078283199 1101 24-25 0.182989883182195 1101 26-27 0.0977464240688306 1101 28-29 0.29232360678044245 1101 30-31 0.3135764485534267 1101 32-33 0.40667410571470697 1101 34-35 0.5240618622449134 1101 36-37 0.3353658839684144 1101 38-39 0.3111379641526568 1101 40-41 0.2592664678180583 1101 42-43 0.16584365890813757 1101 44-45 0.2713034855274472 1101 46-47 0.3063133708633714 1101 48-49 0.40379834825803584 1101 50-51 0.3620212770684148 1101 52-53 0.3596097362640833 1101 54-55 0.34035307970216877 1101 56-57 0.31884857033847425 1101 58-59 0.6237249599424715 1101 60-61 0.4499086264908456 1101 62-63 0.5285979109082675 1101 64-65 0.5878293662359795 1101 66-67 0.4860240646808407 1101 68-69 0.4131459787841365 1101 70-71 0.401668843313697 1101 72-73 0.09211557807413584 1101 74-75 0.07097237938339873 1101 76-77 0.2445677675155764 1101 78-79 0.39111012605597395 1101 80-81 0.35970025338483325 1101 82-83 0.34853940814109663 1101 84-85 0.2256376447392512 1101 86-87 0.3877475112993203 1101 88-89 0.4779051031236925 1101 90-91 0.45984371244360034 1101 92-93 0.3908911779888946 1101 94-95 0.25265670031472 1101 96-97 0.44451959721760304 1101 98-99 0.29072799869259214 1101 100-101 0.15381576246797124 2304 1 -0.2734973077046021 2304 2 -0.3626422614774363 2304 3 -0.3631760256772516 2304 4 -0.13765945627275755 2304 5 -0.2094188704549751 2304 6 -0.3932379954685601 2304 7 0.1377234982590636 2304 8 -0.4932791080650816 2304 9 -0.1628400679004045 2304 10-11 -0.32722724754851384 2304 12-13 -0.3581495938349306 2304 14-15 -0.1435082124917315 2304 16-17 -0.14622782065990236 2304 18-19 -0.14140763373973186 2304 20-21 -0.11162608987187639 2304 22-23 -0.32968529676626446 2304 24-25 -0.21116260360253847 2304 26-27 -0.5126258701781836 2304 28-29 -0.398575465257224 2304 30-31 -0.23425557289967713 2304 32-33 -0.10377222294987831 2304 34-35 -0.11831886276183212 2304 36-37 -0.1375768273793554 2304 38-39 -0.137190835476936 2304 40-41 -0.12070661934480853 2304 42-43 -0.12065916742207605 2304 44-45 -0.07526729812807531 2304 46-47 -0.08008961752262422 2304 48-49 -0.006538013975244894 2304 50-51 -0.16414735741859943 2304 52-53 -0.16083075646269407 2304 54-55 -0.03353616287992622 2304 56-57 0.023897138845626387 2304 58-59 0.16656098455951707 2304 60-61 -0.02675003384792518 2304 62-63 -0.07627557946485553 2304 64-65 0.034037935900677496 2304 66-67 -0.024974748839241556 2304 68-69 -0.17219255383923837 2304 70-71 -0.08973231229844814 2304 72-73 -0.20189655681801355 2304 74-75 -0.15097460522130035 2304 76-77 -0.009310719577833027 2304 78-79 -0.09252923228025267 2304 80-81 0.005212610139086138 2304 82-83 0.06584441064910251 2304 84-85 0.06319093987372071 2304 86-87 -0.06856084807869323 2304 88-89 -0.1491922171680038 2304 90-91 -0.17872870141602704 2304 92-93 -0.11229760736435068 2304 94-95 -0.05269451703296468 2304 96-97 -0.03884605081264425 2304 98-99 -0.037856038811749926 2304 100-101 -0.07452687942731018 2202 1 0.09381258366189371 2202 2 -0.009604811506026323 2202 3 -2.043275640453146E-4 2202 4 0.09808954315548135 2202 5 0.06561686425056479 2202 6 0.07498670435991528 2202 7 0.3765170945998335 2202 8 -0.11627081761339042 2202 9 0.04447125285431497 2202 10-11 -0.020880763843535988 2202 12-13 0.03410312200269772 2202 14-15 0.1715932740720163 2202 16-17 0.19354133314226374 2202 18-19 0.14100731766106378 2202 20-21 0.1455494390017691 2202 22-23 -0.016534925125327504 2202 24-25 0.02422547529968 2202 26-27 -0.027691622036385866 2202 28-29 0.12398028088342983 2202 30-31 0.28241824070810395 2202 32-33 0.2449270337682421 2202 34-35 0.3059021206572581 2202 36-37 0.26784055398142215 2202 38-39 0.29764335548933474 2202 40-41 0.1452989552692543 2202 42-43 0.1631851722005635 2202 44-45 0.20222698431904007 2202 46-47 0.12231890163117498 2202 48-49 0.2910892015764972 2202 50-51 0.2896298009576128 2202 52-53 0.16061364033547676 2202 54-55 0.25235577537050347 2202 56-57 0.3612077449062383 2202 58-59 0.4705668450512306 2202 60-61 0.34699567798740105 2202 62-63 0.2693361986940985 2202 64-65 0.44658796448843674 2202 66-67 0.22403182634657526 2202 68-69 0.20038105965418396 2202 70-71 0.20640833950257687 2202 72-73 0.07805627337066312 2202 74-75 0.22783471438989977 2202 76-77 0.3166827052363459 2202 78-79 0.23835984719373116 2202 80-81 0.26597232997899667 2202 82-83 0.20919775541754504 2202 84-85 0.28919151162901 2202 86-87 0.336184720817819 2202 88-89 0.2785557816884854 2202 90-91 0.3141672391214243 2202 92-93 0.17119653214394148 2202 94-95 0.2344016807943632 2202 96-97 0.4250654700564205 2202 98-99 0.3548790955507428 2202 100-101 0.30775585356296986 1102 1 -0.15498015067344895 1102 2 -0.17301108840465318 1102 3 -0.15921440282502886 1102 4 0.05296716128874834 1102 5 -0.00549704247877969 1102 6 -0.15139050546594746 1102 7 0.13851195213365486 1102 8 -0.26243962513507313 1102 9 0.022311748120152686 1102 10-11 -0.01637186384574818 1102 12-13 -0.09591767960404951 1102 14-15 -0.10510644342340214 1102 16-17 -0.09640878714289158 1102 18-19 -0.17884373290283406 1102 20-21 -0.11525042967774368 1102 22-23 -0.23224496617677204 1102 24-25 -0.1735595949954032 1102 26-27 -0.18870116208361765 1102 28-29 -0.14100942866654975 1102 30-31 0.0011694821833074798 1102 32-33 -0.044191849897252666 1102 34-35 -0.010341478848353347 1102 36-37 -0.07107486569237409 1102 38-39 -0.07012215467421612 1102 40-41 -0.07346952891710856 1102 42-43 -0.04259292928037439 1102 44-45 0.03604670004880717 1102 46-47 0.04093647760945629 1102 48-49 0.01601995484847407 1102 50-51 0.15161983053231154 1102 52-53 0.04186129532675409 1102 54-55 0.1549670115487487 1102 56-57 0.3226878040556329 1102 58-59 0.29038216871760625 1102 60-61 0.23993375320424803 1102 62-63 0.338531750350505 1102 64-65 0.2817037803307656 1102 66-67 0.2548346193899036 1102 68-69 0.14421865262688272 1102 70-71 0.152619771006254 1102 72-73 0.01455178198926177 1102 74-75 -0.00540895684539322 1102 76-77 0.08666893865575531 1102 78-79 0.13723879508487968 1102 80-81 0.21074240877128858 1102 82-83 0.15734671588897342 1102 84-85 0.2361227228673961 1102 86-87 0.12021645292402283 1102 88-89 0.09813136269887934 1102 90-91 0.013238201844060882 1102 92-93 0.0776557896865917 1102 94-95 0.09344858648638876 1102 96-97 0.19674562742030233 1102 98-99 0.04345462690212898 1102 100-101 -0.1856440504296124 2305 1 -0.04361510831921578 2305 2 -0.16199694651637486 2305 3 0.020322525909392652 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-0.02247412807371063 1213 78-79 -0.10945499477433529 1213 80-81 -0.24762621305503174 1213 82-83 -0.3693867649008489 1213 84-85 -0.3483454361901934 1213 86-87 -0.393398845257682 1213 88-89 -0.1806771001250418 1213 90-91 -0.17089333202785895 1213 92-93 -0.07744949029240544 1213 94-95 -0.2187289922959863 1213 96-97 -0.14180630064599242 1213 98-99 -0.13482526371006642 1213 100-101 0.07025672634609847 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2848.0 3 434.0 4 471.0 5 300.0 6 781.0 7 4621.0 8 26997.0 9 81733.0 10 99754.0 11 60704.0 12 31592.0 13 18874.0 14 12655.0 15 9773.0 16 8494.0 17 8115.0 18 8195.0 19 8574.0 20 9195.0 21 10072.0 22 10816.0 23 12015.0 24 13331.0 25 15208.0 26 17944.0 27 21415.0 28 26959.0 29 33526.0 30 40291.0 31 50707.0 32 69794.0 33 99031.0 34 149816.0 35 252575.0 36 489235.0 37 828519.0 38 273454.0 39 473.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.29493749099217 21.93844279009502 23.476160101829535 13.290459617083275 2 31.630500908975755 25.954852717382575 30.970008260172232 11.444638113469432 3 36.32560513898585 20.1445919465582 29.123940184054003 14.405862730401944 4 27.068042246206005 17.928098121693274 35.82530491807021 19.17855471403051 5 34.52270277096597 16.31418075028771 32.12014535138834 17.042971127357983 6 31.32236191134108 16.338417259204572 37.487943770064724 14.851277059389624 7 36.95560579536005 18.442626313725896 28.554930947224065 16.046836943689996 8 27.461009594839375 32.92273404052676 24.21057850838737 15.405677856246491 9 33.37292530065819 27.85227131269486 22.46455359700157 16.310249789645383 10-11 27.015368374689835 30.875659443878284 26.568145728961323 15.540826452470558 12-13 29.991256599020353 23.840018949386373 26.579689268590073 19.5890351830032 14-15 26.273388105936185 24.221751990833816 28.621758697100752 20.883101206129243 16-17 27.896014483059993 25.14376934121697 29.791083643238714 17.169132532484326 18-19 27.881956872726015 25.277506250478183 30.266510509488654 16.57402636730715 20-21 25.855576231878374 28.43095836985374 29.145578452081505 16.567886946186377 22-23 29.71460528452741 25.797267012270115 27.87965883202578 16.608468871176697 24-25 25.222290168687085 30.248329324809873 27.782707324607053 16.746673181895986 26-27 22.264847541852586 33.37292844518551 27.681162371034596 16.68106164192731 28-29 24.388970239504637 31.05492990692647 27.527152705398777 17.02894714817011 30-31 28.031384081333815 27.132005346605464 27.72342046353077 17.11319010852995 32-33 27.71206034135994 26.652624930607004 28.755616634884618 16.879698093148438 34-35 23.553328108064466 26.704315276623685 28.207467228347344 21.534889386964505 36-37 19.481064893951295 26.991799452701397 29.09367759185758 24.433458061489723 38-39 19.294059899539278 28.743551513850278 31.145645538096815 20.816743048513622 40-41 21.13817739850678 26.90185684163292 33.9992368850333 17.960728874826994 42-43 22.948033225391683 26.37639906216231 33.19411374231054 17.48145397013547 44-45 21.353288978060736 28.563399736426565 32.701506213019286 17.381805072493414 46-47 23.968922543467038 30.307202751958016 28.35698193026941 17.36689277430553 48-49 20.41569162911689 29.764438313155413 30.749198692894307 19.070671364833398 50-51 19.22286310226805 27.221092536371387 32.89274691647586 20.663297444884705 52-53 25.37770931970415 26.516917906962906 28.351078402305795 19.75429437102715 54-55 27.809282825850367 26.68662370133977 27.66484829819147 17.839245174618394 56-57 28.819496370909604 26.409072091711472 27.056435424381934 17.714996112996992 58-59 26.531748779800157 26.41863529862424 27.798866328130224 19.250749593445384 60-61 21.713259687452673 26.645834702525693 27.346194454842372 24.29471115517926 62-63 24.43382811725924 26.697337378725273 27.382572501510772 21.486262002504716 64-65 21.37744235552564 28.379315400270677 30.406307316124558 19.836934928079117 66-67 19.408244226057477 28.565810559357296 29.158970070920898 22.86697514366433 68-69 18.856725912187628 27.325815721911596 34.81112309343156 19.006335272469215 70-71 18.277891388118015 31.83305201012942 31.766926924103434 18.12212967764913 72-73 18.169550026624005 36.2987222440494 27.209252551772224 18.322475177554367 74-75 18.54283685737662 36.75665311606429 26.489228815349104 18.21128121120998 76-77 18.785995949761038 36.31182233794447 26.710010138987506 18.192171573306986 78-79 19.11367767022743 35.841406454080385 26.86133813616837 18.18357773952381 80-81 18.56785519520384 35.95302693894052 26.797551349554098 18.68156651630154 82-83 18.451056749566355 35.15687287285571 27.035878905301182 19.356191472276755 84-85 18.6794589248529 34.07854793827473 27.581783698571204 19.66020943830117 86-87 18.796965481214343 33.71540247142878 27.938611434132245 19.54902061322463 88-89 19.265233439133326 33.953579606082464 27.390526306575115 19.390660648209103 90-91 19.795060923609963 33.28267751340587 27.002897722452662 19.919363840531503 92-93 19.56018561917429 33.249662217598825 27.329529029466837 19.860623133760043 94-95 19.144014799769142 33.22614580657439 27.42261334889049 20.20722604476598 96-97 19.504404061309945 33.813403065568764 26.959697031862824 19.722495841258468 98-99 19.984269094217375 33.00668367038037 26.84843054388964 20.160616691512615 100-101 20.179271268367447 32.87475304650407 26.337103579352007 20.60887210577648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2195.0 1 1385.5 2 459.0 3 362.0 4 351.5 5 347.5 6 439.5 7 580.5 8 717.0 9 866.5 10 1010.5 11 1097.0 12 1084.0 13 1004.0 14 948.0 15 935.5 16 918.0 17 904.5 18 971.5 19 1094.0 20 1252.0 21 1532.0 22 1980.5 23 2697.5 24 3720.0 25 5358.0 26 8163.0 27 12124.5 28 16410.5 29 20652.5 30 25246.0 31 29773.0 32 36308.5 33 47316.5 34 63166.5 35 85717.0 36 110377.0 37 137710.5 38 168374.0 39 183261.0 40 179074.0 41 164212.5 42 150009.0 43 147435.5 44 141672.0 45 134036.0 46 136466.5 47 129630.5 48 108207.0 49 90402.5 50 78443.0 51 68220.0 52 59971.5 53 51888.5 54 43737.0 55 35603.5 56 27669.0 57 21223.5 58 16104.0 59 12288.5 60 9348.0 61 7007.5 62 5085.0 63 3603.0 64 2619.5 65 1912.5 66 1370.0 67 1005.0 68 756.5 69 565.5 70 406.0 71 322.0 72 245.5 73 183.0 74 139.5 75 110.5 76 95.5 77 76.0 78 64.5 79 55.5 80 50.5 81 43.0 82 33.5 83 32.0 84 24.5 85 19.5 86 17.5 87 15.0 88 13.5 89 14.5 90 13.5 91 8.0 92 5.0 93 7.0 94 5.5 95 3.0 96 3.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21980634971599597 2 0.23799599258318202 3 0.21685186760645303 4 0.2105513455174277 5 0.2190232339761171 6 0.24198276362256527 7 0.22389990926536268 8 0.21033776849746075 9 0.20261339960865574 10-11 0.18931823011571247 12-13 0.21478728974677241 14-15 0.21154803827727353 16-17 0.21414655868687155 18-19 0.20309394790358135 20-21 0.19554755986474878 22-23 0.1648636613294956 24-25 0.49085338613906493 26-27 0.34538963745656825 28-29 0.4783057362160061 30-31 0.30739072598744666 32-33 0.3565668348348391 34-35 0.3585602203545307 36-37 0.36559046392844313 38-39 0.3590763648194509 40-41 0.3643979922336276 42-43 0.41975003657506466 44-45 0.3719977745274519 46-47 0.39129089866446726 48-49 0.38780247400500695 50-51 0.4027884615726886 52-53 0.40834146409182953 54-55 0.3718019955924822 56-57 0.38666339656518317 58-59 0.3835131355206705 60-61 0.34126048173720697 62-63 0.3051837634477881 64-65 0.33120456371376267 66-67 0.3425241457720115 68-69 0.3322012564736085 70-71 0.3039378974979808 72-73 0.29357941202958326 74-75 0.3045074362178927 76-77 0.3102384195870061 78-79 0.3203833280354367 80-81 0.328125495009239 82-83 0.3475254076562378 84-85 0.3761447283318104 86-87 0.37826270044648275 88-89 0.3722113515474189 90-91 0.34451753129170315 92-93 0.3218427710052109 94-95 0.3003070881585425 96-97 0.3155422489161856 98-99 0.34551422405154897 100-101 0.36240460671393604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2809291.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.4939908457781 #Duplication Level Percentage of deduplicated Percentage of total 1 92.21369463938665 57.62801788649207 2 3.8071727516358274 4.758508381780505 3 1.0416172452736439 1.952844557728071 4 0.7358483079484407 1.8394438968324462 5 0.4291308675387081 1.340905025380242 6 0.2896436346623753 1.0860591991877029 7 0.2005236213283986 0.8772064948961452 8 0.13355398862329068 0.6677057393952864 9 0.12098368546098759 0.680467799851871 >10 0.8624050118440058 10.608419996788996 >50 0.08406426558603411 3.653643684088453 >100 0.07123485156990296 9.474161209441748 >500 0.008137674495026429 3.325089078159233 >1k 0.0019321288553842669 1.838730763507497 >5k 5.732579134068816E-5 0.2687962864697745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 7503 0.267078063468683 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3379 0.12027945841139277 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3323 0.11828607289170114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005837771879096897 0.0 0.0 2 0.006336118259019802 0.0 0.0 3 0.006763272298953722 0.0 0.0 4 0.007154830168893148 0.0 0.0 5 0.008365099948705918 0.0 0.0 6 0.009290600368562744 0.0 0.0 7 0.010607658658358995 0.0 0.0 8 0.01185352460816626 0.0 0.0 9 0.012992602047990045 0.0 0.0 10-11 0.02009403796189145 0.0 0.0 12-13 0.024650347721186593 0.0 0.0 14-15 0.03011435981534131 0.0 0.0 16-17 0.03504442935957863 0.0 0.0 18-19 0.04045504719874161 0.0 0.0 20-21 0.045652088017937624 0.0 0.0 22-23 0.05127628287706756 0.0 0.0 24-25 0.05777258390106258 0.0 0.0 26-27 0.06387732705511817 0.0 3.559616999449327E-5 28-29 0.07122793615898104 0.0 3.559616999449327E-5 30-31 0.13820213000362014 0.0 3.559616999449327E-5 32-33 0.14936152929689378 0.0 3.559616999449327E-5 34-35 0.15804699477555012 0.0 3.559616999449327E-5 36-37 0.16806731662899999 0.0 3.559616999449327E-5 38-39 0.17922671592227363 0.0 3.559616999449327E-5 40-41 0.19102684627544814 0.0 3.559616999449327E-5 42-43 0.20286257279861716 0.0 3.559616999449327E-5 44-45 0.21596196335659068 0.0 3.559616999449327E-5 46-47 0.23018263326939076 0.0 3.559616999449327E-5 48-49 0.24516862083707242 0.0 3.559616999449327E-5 50-51 0.26147166669455035 0.0 3.559616999449327E-5 52-53 0.27845103978192365 0.0 3.559616999449327E-5 54-55 0.2972636156240133 0.0 3.559616999449327E-5 56-57 0.3178915961358222 0.0 3.559616999449327E-5 58-59 0.3394806732374823 0.0 3.559616999449327E-5 60-61 0.36416661712866344 0.0 3.559616999449327E-5 62-63 0.38917292654979496 0.0 3.559616999449327E-5 64-65 0.41734729510043633 0.0 3.559616999449327E-5 66-67 0.4468565200258713 0.0 3.559616999449327E-5 68-69 0.48065508343564267 0.0 3.559616999449327E-5 70-71 0.5146316276953864 0.0 7.119233998898654E-5 72-73 0.5517050387446513 0.0 7.119233998898654E-5 74-75 0.5944026446530459 0.0 7.119233998898654E-5 76-77 0.6394495977810771 0.0 1.4238467997797308E-4 78-79 0.6888392836484365 0.0 1.4238467997797308E-4 80-81 0.7418953750252288 0.0 1.95778934969713E-4 82-83 0.8005222670061591 0.0 2.1357701996695962E-4 84-85 0.863296112791448 0.0 2.1357701996695962E-4 86-87 0.9342748757604677 0.0 2.1357701996695962E-4 88-89 1.010646458483653 0.0 2.1357701996695962E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3215 0.0 86.58648 9 CGGGAGA 4105 0.0 84.92525 4 AAGCGGC 1305 0.0 84.671715 9 CGGAAAG 2000 0.0 82.29286 5 GAGAGCG 3940 0.0 80.165306 7 ATCGGGA 5335 0.0 79.39679 2 ATTCGGA 835 0.0 78.96129 2 TCGGGAG 4380 0.0 78.618576 3 GATCGGG 5600 0.0 77.678024 1 GTTCGGA 745 0.0 75.1297 2 ATCCGGA 885 0.0 74.5002 2 AGAGCGG 4010 0.0 72.73169 8 TCGGAGA 1260 0.0 72.652306 4 GGTCCGG 585 0.0 72.16906 1 TCGGGAA 1720 0.0 70.59504 3 TTCGGAG 765 0.0 70.06154 3 GATCCGG 960 0.0 69.67304 1 AAAGCGG 1690 0.0 69.591774 8 AAGGGGC 3675 0.0 68.90914 9 AAGCGTC 510 0.0 67.8806 9 >>END_MODULE