FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809291
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT75030.267078063468683Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33790.12027945841139277No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33230.11828607289170114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC32150.086.586489
CGGGAGA41050.084.925254
AAGCGGC13050.084.6717159
CGGAAAG20000.082.292865
GAGAGCG39400.080.1653067
ATCGGGA53350.079.396792
ATTCGGA8350.078.961292
TCGGGAG43800.078.6185763
GATCGGG56000.077.6780241
GTTCGGA7450.075.12972
ATCCGGA8850.074.50022
AGAGCGG40100.072.731698
TCGGAGA12600.072.6523064
GGTCCGG5850.072.169061
TCGGGAA17200.070.595043
TTCGGAG7650.070.061543
GATCCGG9600.069.673041
AAAGCGG16900.069.5917748
AAGGGGC36750.068.909149
AAGCGTC5100.067.88069
Produced by FastQC (version 0.11.2)