FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863300
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT173020.35576666049801575Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC159500.088.928229
ATCGGGA248650.087.475492
GAGAGCG186650.085.6224067
TCGGGAG229100.084.484333
AGAGCGG177850.081.727928
TCCGGAG35700.081.4180153
GATCGGG273350.081.1779861
CGGAGAG99150.080.390225
GGATCGG71750.079.382621
CGGGAGA243500.079.079194
ATTGGGA110250.078.105822
GGAGAGC208550.078.029876
TCCGGAA26250.077.311253
GAGCGTC228850.076.853169
CCGGAGA48550.076.667034
GAGGGGC221600.075.4526449
TCGGAGA61900.075.375944
ATCCGGA46000.074.941562
AGAGCGT244400.072.64458
CGGAAAG84000.072.535755
Produced by FastQC (version 0.11.2)