##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_30521-1.342000000356dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3032762 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76181546722097 33.0 33.0 33.0 27.0 33.0 2 31.611204571938057 33.0 33.0 33.0 27.0 33.0 3 31.565851524122237 33.0 33.0 33.0 27.0 33.0 4 35.1997970826593 37.0 37.0 37.0 33.0 37.0 5 35.19764887584321 37.0 37.0 37.0 33.0 37.0 6 35.29423476026144 37.0 37.0 37.0 33.0 37.0 7 35.15131586322963 37.0 37.0 37.0 33.0 37.0 8 35.131465640891044 37.0 37.0 37.0 33.0 37.0 9 34.931512924522266 37.0 37.0 37.0 33.0 37.0 10-11 34.887613172415115 37.0 37.0 37.0 33.0 37.0 12-13 34.932711666790865 37.0 37.0 37.0 33.0 37.0 14-15 36.62872688328329 40.0 37.0 40.0 33.0 40.0 16-17 36.55070114305046 40.0 37.0 40.0 33.0 40.0 18-19 36.531552756200455 40.0 37.0 40.0 33.0 40.0 20-21 36.46846191689292 40.0 37.0 40.0 33.0 40.0 22-23 36.66117403871454 40.0 37.0 40.0 33.0 40.0 24-25 36.355434089453766 40.0 37.0 40.0 33.0 40.0 26-27 36.24187918471677 40.0 37.0 40.0 33.0 40.0 28-29 36.06560504912684 40.0 37.0 40.0 30.0 40.0 30-31 36.104254141933986 40.0 37.0 40.0 33.0 40.0 32-33 36.01944531090801 40.0 37.0 40.0 27.0 40.0 34-35 35.91579804152123 38.5 37.0 40.0 27.0 40.0 36-37 35.826992358780544 37.0 37.0 40.0 27.0 40.0 38-39 35.73080198841848 37.0 37.0 40.0 27.0 40.0 40-41 35.63950616632627 37.0 37.0 40.0 27.0 40.0 42-43 35.522728127034036 37.0 37.0 40.0 27.0 40.0 44-45 35.39249568545108 37.0 37.0 40.0 27.0 40.0 46-47 35.24255694973756 37.0 37.0 40.0 27.0 40.0 48-49 35.09494513582008 37.0 37.0 40.0 27.0 40.0 50-51 33.913734081342355 37.0 33.0 38.5 24.5 38.5 52-53 34.374676944646495 37.0 33.0 38.5 27.0 40.0 54-55 35.089821588373894 37.0 37.0 40.0 27.0 40.0 56-57 35.13604018383243 37.0 37.0 40.0 27.0 40.0 58-59 34.855175414358264 37.0 33.0 40.0 27.0 40.0 60-61 34.59126284884867 37.0 33.0 40.0 27.0 40.0 62-63 34.300138124917154 37.0 33.0 38.5 27.0 40.0 64-65 34.04410979166845 37.0 33.0 37.0 27.0 40.0 66-67 33.8191991326718 37.0 33.0 37.0 27.0 40.0 68-69 33.67594374368974 37.0 33.0 37.0 27.0 40.0 70-71 33.369853453716445 37.0 33.0 37.0 24.5 38.5 72-73 33.231741066394264 37.0 33.0 37.0 24.5 37.0 74-75 33.080463122394704 37.0 33.0 37.0 27.0 37.0 76-77 32.85196447990313 37.0 33.0 37.0 22.0 37.0 78-79 32.614326313769425 37.0 33.0 37.0 22.0 37.0 80-81 32.36881397221411 37.0 33.0 37.0 22.0 37.0 82-83 32.144694506196004 35.0 33.0 37.0 22.0 37.0 84-85 31.99398469118249 33.0 33.0 37.0 22.0 37.0 86-87 31.794889773744195 33.0 33.0 37.0 22.0 37.0 88-89 31.6843262016604 33.0 33.0 37.0 22.0 37.0 90-91 31.5764952871343 33.0 33.0 37.0 15.0 37.0 92-93 31.415119617035558 33.0 33.0 37.0 15.0 37.0 94-95 31.282158804416568 33.0 33.0 37.0 15.0 37.0 96-97 31.088974011148913 33.0 33.0 37.0 15.0 37.0 98-99 30.952266778599835 33.0 33.0 37.0 15.0 37.0 100-101 29.55147584940724 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11076439646010883 2306 2 0.15958513828396548 2306 3 0.09725422483185753 2306 4 0.12153577195598331 2306 5 0.13956972114217336 2306 6 0.10939311439546628 2306 7 0.26785149368689076 2306 8 0.13990061359854167 2306 9 0.27321012522439503 2306 10-11 0.24394935675420015 2306 12-13 0.13517803655093275 2306 14-15 0.3408767184368031 2306 16-17 0.36847525577682916 2306 18-19 0.31491020257274727 2306 20-21 0.363876485713881 2306 22-23 0.3099236018117608 2306 24-25 0.3376725000250076 2306 26-27 0.2507281265452761 2306 28-29 0.23787814671777596 2306 30-31 0.3518232401032435 2306 32-33 0.3592761406999543 2306 34-35 0.45933173238121583 2306 36-37 0.46995013599752866 2306 38-39 0.30665670440627935 2306 40-41 0.27306647241647397 2306 42-43 0.23997078474511824 2306 44-45 0.37578562781300207 2306 46-47 0.23999400897879752 2306 48-49 0.3401945706216125 2306 50-51 0.1985324112148774 2306 52-53 0.32447688415849996 2306 54-55 0.3499000365863978 2306 56-57 0.4134457959444191 2306 58-59 0.35465872995838055 2306 60-61 0.23165849372253433 2306 62-63 0.3263211804921369 2306 64-65 0.32747452308300495 2306 66-67 0.20392091063185092 2306 68-69 0.14570046264675796 2306 70-71 0.2911683425905309 2306 72-73 0.11922352350006094 2306 74-75 0.09189838976787712 2306 76-77 0.19094843810442796 2306 78-79 0.2134928490713861 2306 80-81 0.15993153883823652 2306 82-83 0.15886031314759208 2306 84-85 0.17426893376819663 2306 86-87 0.25330242950439086 2306 88-89 0.20313114141449873 2306 90-91 0.15242505243287496 2306 92-93 0.2028661414236872 2306 94-95 0.18094694774170605 2306 96-97 0.31885220632763733 2306 98-99 0.27641260044755356 2306 100-101 -0.09318393123191271 2307 1 0.12129542853215014 2307 2 0.16748968885628912 2307 3 0.09072664581049139 2307 4 0.1435383482253343 2307 5 0.18565095280749944 2307 6 0.13380780492448707 2307 7 0.20574679363686954 2307 8 0.07846798381462605 2307 9 0.28004477083285906 2307 10-11 0.2971529882511206 2307 12-13 0.15605856447371735 2307 14-15 0.3244032719716401 2307 16-17 0.34439949723545027 2307 18-19 0.29162045724489616 2307 20-21 0.23446626599712062 2307 22-23 0.1693913916855081 2307 24-25 0.1448395114173664 2307 26-27 0.06661949836041714 2307 28-29 0.11995974918049512 2307 30-31 0.33684457060924444 2307 32-33 0.38045697588245275 2307 34-35 0.49022572102168027 2307 36-37 0.3680532756085384 2307 38-39 0.35187757025680355 2307 40-41 0.25841321625534874 2307 42-43 0.37606984172239066 2307 44-45 0.3151728078795557 2307 46-47 0.3410671970445307 2307 48-49 0.19571587891839926 2307 50-51 0.1028792380969179 2307 52-53 0.22914526190061935 2307 54-55 0.1780219285028224 2307 56-57 0.2595996420982587 2307 58-59 0.26651420395138103 2307 60-61 0.4094017667433576 2307 62-63 0.3072038713996861 2307 64-65 0.25489576243465706 2307 66-67 0.27177490143530036 2307 68-69 0.17664756028813855 2307 70-71 0.18160833617881877 2307 72-73 0.011126428658961629 2307 74-75 -0.022343899287996294 2307 76-77 0.04206459617999059 2307 78-79 0.04448745512812735 2307 80-81 0.05964436398602402 2307 82-83 -0.019797400307133728 2307 84-85 0.20387189087737312 2307 86-87 0.07584497947030044 2307 88-89 0.0325672396989809 2307 90-91 -0.08737560534624222 2307 92-93 -0.13270163242080812 2307 94-95 -0.17493743299769093 2307 96-97 -0.14981155189701667 2307 98-99 -0.13702745300907182 2307 100-101 -0.10399113010870664 2201 1 0.23900683672643552 2201 2 0.12880606462894306 2201 3 0.11266704638854463 2201 4 0.3094001383250884 2201 5 0.18603318806976432 2201 6 0.22515978037777984 2201 7 0.29184544733541884 2201 8 0.21596923661643785 2201 9 0.28750961958690624 2201 10-11 0.2601452629482921 2201 12-13 0.11641830641343631 2201 14-15 0.37842939492044536 2201 16-17 0.4200181319766827 2201 18-19 0.42367083572376174 2201 20-21 0.34541737061037736 2201 22-23 0.22585120783614343 2201 24-25 0.18388220513350717 2201 26-27 0.2576984394336108 2201 28-29 0.24185048813166077 2201 30-31 0.4720795190098954 2201 32-33 0.41640092565004494 2201 34-35 0.4864502630029648 2201 36-37 0.34953553762405676 2201 38-39 0.41561087622019954 2201 40-41 0.36753921184065774 2201 42-43 0.3877129755560489 2201 44-45 0.4157834512333025 2201 46-47 0.455648490972024 2201 48-49 0.4436462020632419 2201 50-51 0.3284919124776806 2201 52-53 0.4365366880226276 2201 54-55 0.5340343395659559 2201 56-57 0.5145586057001665 2201 58-59 0.6509453080562793 2201 60-61 0.6121474096072461 2201 62-63 0.6354215370072183 2201 64-65 0.6583979286757895 2201 66-67 0.45628497830545456 2201 68-69 0.4071251275344352 2201 70-71 0.4814755190775841 2201 72-73 0.4478677797415713 2201 74-75 0.4006465543219804 2201 76-77 0.48403232271833474 2201 78-79 0.503244012932285 2201 80-81 0.4602710785951203 2201 82-83 0.3599001640852677 2201 84-85 0.2923470472744967 2201 86-87 0.2930000551797498 2201 88-89 0.5200057599079848 2201 90-91 0.3246818901730322 2201 92-93 0.14030274478426108 2201 94-95 0.20717106100663685 2201 96-97 0.40705117175123107 2201 98-99 0.4366031079227817 2201 100-101 0.2498749076930089 1101 1 0.20164780766146606 1101 2 0.18227397271453682 1101 3 0.19111111864691566 1101 4 0.3095255351793256 1101 5 0.2530314465129777 1101 6 0.2478972681694529 1101 7 0.390175643761701 1101 8 0.3204292840170453 1101 9 0.39850028154230444 1101 10-11 0.3332991368026299 1101 12-13 0.2617152839918191 1101 14-15 0.4724141137797986 1101 16-17 0.46037976042014606 1101 18-19 0.397189611445377 1101 20-21 0.27665543966562467 1101 22-23 0.12170800343717758 1101 24-25 0.17871405271765184 1101 26-27 0.31718867292493513 1101 28-29 0.14743998589194973 1101 30-31 0.29114972963193964 1101 32-33 0.4705728879261457 1101 34-35 0.3816956372440785 1101 36-37 0.2727404637843662 1101 38-39 0.2286528043412943 1101 40-41 0.3397227046702511 1101 42-43 0.3432084107829141 1101 44-45 0.1988769022708894 1101 46-47 0.23775946808158466 1101 48-49 0.22797351644685904 1101 50-51 0.3899214038519503 1101 52-53 0.39997806916324663 1101 54-55 0.4130166949477925 1101 56-57 0.4118360195821609 1101 58-59 0.5765717934207331 1101 60-61 0.55587512448637 1101 62-63 0.4631210468276379 1101 64-65 0.5467082015971201 1101 66-67 0.5609481626528279 1101 68-69 0.4632732816838043 1101 70-71 0.4522981879694328 1101 72-73 0.25606800426449183 1101 74-75 0.5366007960865176 1101 76-77 0.4110872450512133 1101 78-79 0.5229911878543376 1101 80-81 0.37685379986174183 1101 82-83 0.1531084649400256 1101 84-85 0.2709530333807564 1101 86-87 0.24290840526989044 1101 88-89 0.4585928739366061 1101 90-91 0.48641955697542016 1101 92-93 0.16830109228605394 1101 94-95 0.2678539049596331 1101 96-97 0.4153370444105775 1101 98-99 0.2539099438980408 1101 100-101 0.3282007316114033 2304 1 0.14229644621645576 2304 2 0.14977860016751876 2304 3 0.12445986527970376 2304 4 0.3112834270610776 2304 5 0.21372697880790525 2304 6 0.20640755661609944 2304 7 0.4093390202107159 2304 8 0.19441156989708475 2304 9 0.3851504156213892 2304 10-11 0.32934090176486563 2304 12-13 0.22589196861050453 2304 14-15 0.2979724349251782 2304 16-17 0.37887806084760456 2304 18-19 0.3276352737467292 2304 20-21 0.2767535851658707 2304 22-23 0.230972065948869 2304 24-25 0.33106489025824715 2304 26-27 0.2363731606996211 2304 28-29 0.2556498673776204 2304 30-31 0.36240668743450044 2304 32-33 0.48609989393843733 2304 34-35 0.4708480452234909 2304 36-37 0.3184414276743013 2304 38-39 0.2510305325022273 2304 40-41 0.32805523409243165 2304 42-43 0.3811000542719114 2304 44-45 0.4985616592011297 2304 46-47 0.3390954243539923 2304 48-49 0.31417913521787 2304 50-51 0.1668376514551113 2304 52-53 0.27364444704987534 2304 54-55 0.3913230069848268 2304 56-57 0.47859345294365596 2304 58-59 0.4652096197631792 2304 60-61 0.5387872102216278 2304 62-63 0.31817558857472505 2304 64-65 0.30003801452917145 2304 66-67 0.3394616815201701 2304 68-69 0.33731291722807555 2304 70-71 0.4443844191462034 2304 72-73 0.3879149515861897 2304 74-75 0.27491725652065213 2304 76-77 0.33925332746240144 2304 78-79 0.37250539187009224 2304 80-81 0.46354592109837967 2304 82-83 0.36341543248094155 2304 84-85 0.3257732172487735 2304 86-87 0.20359832727158178 2304 88-89 0.3330081913392995 2304 90-91 0.1827216395439777 2304 92-93 0.28216483336256815 2304 94-95 0.2942240840208008 2304 96-97 0.24409953562458142 2304 98-99 0.27290494228698847 2304 100-101 0.24274585319130537 2202 1 0.07661878963676472 2202 2 -0.04379761051156095 2202 3 -0.040102765766643955 2202 4 0.03703606514930158 2202 5 0.032111113646514866 2202 6 0.10865522832453678 2202 7 0.05440136806361551 2202 8 -0.012622039084668302 2202 9 0.013924834277368348 2202 10-11 0.013183908864540683 2202 12-13 0.024396995597136595 2202 14-15 0.03771415280888135 2202 16-17 0.13342890099240634 2202 18-19 0.06702419601545984 2202 20-21 0.09837597545122634 2202 22-23 0.002862395891241931 2202 24-25 -0.15858365258102936 2202 26-27 -0.07270138048425423 2202 28-29 -0.06643728168803875 2202 30-31 0.038640154685552375 2202 32-33 0.1458710756787056 2202 34-35 0.19880300834326903 2202 36-37 0.1884088747827164 2202 38-39 0.14699748531398882 2202 40-41 -0.041966567783418895 2202 42-43 0.10911262578958514 2202 44-45 0.12420053505777418 2202 46-47 -0.005437691798441335 2202 48-49 -0.009466120051129678 2202 50-51 0.05095781683140643 2202 52-53 0.09831383034910601 2202 54-55 0.065165311045142 2202 56-57 0.17416771169965273 2202 58-59 0.21330519330741282 2202 60-61 0.13127784391272712 2202 62-63 0.17012047488203308 2202 64-65 0.15166302430601064 2202 66-67 0.17449004998782414 2202 68-69 0.15710019614548543 2202 70-71 0.22400326973681928 2202 72-73 0.13657672062461756 2202 74-75 0.2314694876881589 2202 76-77 0.20079425449699784 2202 78-79 0.11456873456835126 2202 80-81 0.12792743338325607 2202 82-83 0.1350449616142697 2202 84-85 0.1416492495906212 2202 86-87 0.3516143336022459 2202 88-89 0.19412493427914157 2202 90-91 -0.08587786649112772 2202 92-93 0.08081665649930159 2202 94-95 0.04007650266122198 2202 96-97 0.06163236762662905 2202 98-99 -0.09812491085689601 2202 100-101 0.07411650307145479 1102 1 -0.17833446395516717 1102 2 -0.20326082243465748 1102 3 -0.22611917182543095 1102 4 -0.17678049068079105 1102 5 -0.19184586396953307 1102 6 -0.2327424267345819 1102 7 -0.11955041537621725 1102 8 -0.1802144312301337 1102 9 -0.03388455429624315 1102 10-11 -0.07002490950394957 1102 12-13 -0.19664564870732448 1102 14-15 -0.26817989447673796 1102 16-17 -0.12338889696427202 1102 18-19 -0.2167901855630916 1102 20-21 -0.12165992914460588 1102 22-23 -0.23500231242573477 1102 24-25 -0.32503161812468306 1102 26-27 -0.20620501840989647 1102 28-29 -0.1338911978024413 1102 30-31 -0.12533633701471558 1102 32-33 -0.10287036718099785 1102 34-35 -0.17362973178608598 1102 36-37 -0.1859322245311148 1102 38-39 -0.15694656693023035 1102 40-41 -0.2517652928663878 1102 42-43 -0.09631892780618756 1102 44-45 0.01330699305596994 1102 46-47 -0.03839155974011277 1102 48-49 -0.10779175791599016 1102 50-51 0.07410529209663963 1102 52-53 0.008412887176220352 1102 54-55 0.07031696803445442 1102 56-57 0.12962199717770062 1102 58-59 0.26084621611020253 1102 60-61 0.20857872852451464 1102 62-63 0.22955816349482205 1102 64-65 0.23209231060264557 1102 66-67 0.16895306992662285 1102 68-69 0.10821752116122241 1102 70-71 0.1208411000175218 1102 72-73 0.09176128584145005 1102 74-75 0.06802213060515783 1102 76-77 0.03810694319811603 1102 78-79 0.06988444978993158 1102 80-81 0.06085342237913238 1102 82-83 -0.023098034410132584 1102 84-85 -0.0716170297938703 1102 86-87 0.021136226760521737 1102 88-89 0.05379572479978023 1102 90-91 0.02792806542299786 1102 92-93 -0.011649927044842912 1102 94-95 -8.536058699704085E-4 1102 96-97 0.1570264171500959 1102 98-99 0.06641157428528643 1102 100-101 0.05740939406983969 2305 1 0.03772374266482004 2305 2 0.05033991814525507 2305 3 -0.061442462198559866 2305 4 -0.03245089991273176 2305 5 -0.016964231732785606 2305 6 -0.03821420966306732 2305 7 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-0.4758144058986531 1213 72-73 -0.33654589759391484 1213 74-75 -0.3650659257696134 1213 76-77 -0.45442816936648 1213 78-79 -0.44340698206298157 1213 80-81 -0.3004116357119173 1213 82-83 -0.4038921347855009 1213 84-85 -0.39010242835270503 1213 86-87 -0.39696138438465667 1213 88-89 -0.2590315139202346 1213 90-91 -0.22474159258435122 1213 92-93 -0.22433019425397305 1213 94-95 -0.29157558313967513 1213 96-97 -0.28352320114575136 1213 98-99 -0.2930188543329528 1213 100-101 -0.10096644095869678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3169.0 3 325.0 4 321.0 5 234.0 6 580.0 7 2770.0 8 11550.0 9 26161.0 10 31047.0 11 21197.0 12 12826.0 13 9090.0 14 7345.0 15 6568.0 16 6913.0 17 7323.0 18 8076.0 19 9127.0 20 10311.0 21 11765.0 22 13014.0 23 14610.0 24 16540.0 25 19313.0 26 22905.0 27 27442.0 28 33623.0 29 41477.0 30 52512.0 31 68712.0 32 93729.0 33 135197.0 34 205809.0 35 351122.0 36 652351.0 37 893268.0 38 204380.0 39 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.53620864629539 25.007542813937206 19.065556884383287 16.390691655384117 2 33.95580525384413 22.93577496481594 28.655093111328373 14.453326670011549 3 39.778847783868024 19.718513715938165 22.965146154306247 17.537492345887564 4 33.450624991945766 18.549583839855085 29.19855179111269 18.80123937708646 5 35.7851993164838 16.909491602604923 28.061968522697565 19.243340558213713 6 32.02904600934684 17.38694992111805 31.507132759327916 19.07687131020719 7 36.50926049547055 17.84038318926943 24.608919051465115 21.041437263794908 8 31.73803959250339 25.539580112352013 22.476350466117783 20.246029829026817 9 31.209647283265372 23.798971318575042 24.074033079439488 20.91734831872009 10-11 25.31004487414443 28.522212346360938 28.522361007346657 17.645381772147967 12-13 26.178986228473317 25.964049825460204 28.112406005596373 19.744557940470102 14-15 25.630809325022497 26.024057848275326 28.143902326596514 20.201230500105655 16-17 25.70682101423762 26.12768932837031 28.63657534497802 19.528914312414052 18-19 25.26001940791006 26.104911086727324 28.552673444567024 20.082396060795592 20-21 24.363893219629503 27.079308883177706 28.18765360638639 20.369144290806396 22-23 25.204062018207544 26.475548227796423 27.73451691615837 20.58587283783766 24-25 24.19493373239259 26.784450341378584 28.28547891750316 20.735137008725665 26-27 23.114082145365415 27.54671561648731 28.72199110399328 20.617211134153997 28-29 23.348346171858704 28.014099157625527 27.79666784231434 20.84088682820143 30-31 23.67508511199556 27.943064064917483 27.83389247731518 20.54795834577177 32-33 23.570199129826715 27.613274924653265 28.230279210655468 20.586246734864545 34-35 22.949051547466475 27.477001256770965 28.105816971455 21.468130224307554 36-37 21.413961588603076 27.959782183834434 28.78225234465473 21.84400388290776 38-39 21.34571575747477 28.13532083562996 28.879914391101412 21.639049015793862 40-41 21.98894813686597 27.444616159324493 29.317108180208944 21.24932752360059 42-43 22.92599179890597 27.132057614953897 28.78568772329404 21.15626286284609 44-45 22.1453493923902 27.624421033399216 28.7677657743626 21.462463799847974 46-47 22.639400367867598 28.104098055651562 28.393224376723964 20.863277199756876 48-49 21.68088347119777 28.305295325188922 28.691328990661503 21.322492212951808 50-51 21.48418248600169 27.826435038790336 28.049676262976675 22.6397062122313 52-53 23.046921756676504 27.06248602085312 27.65206455961155 22.23852766285882 54-55 23.898674313105587 26.9449589126928 27.940792175000006 21.215574599201606 56-57 24.483820975288324 27.040579108994585 26.971159917882098 21.504439997834996 58-59 23.773291038779295 27.561130830363034 26.978684173721046 21.686893957136625 60-61 22.545013375502272 27.327038765215057 27.086264834117795 23.041683025164872 62-63 23.178016509052092 27.123389213034116 27.488311008300503 22.210283269613285 64-65 22.72156776723294 27.746564545550946 28.151743786041273 21.380123901174844 66-67 22.491305400206375 27.671865696248794 27.81780559402634 22.019023309518488 68-69 22.67226898541211 26.934654429750278 29.132614421664094 21.260462163173514 70-71 22.22268162291564 28.144016604578663 28.50615849599563 21.12714327651006 72-73 22.090417968167987 29.586156066386614 27.07262207874311 21.25080388670229 74-75 22.461363304839498 29.145611626252343 26.883905839687195 21.50911922922096 76-77 22.154586011312137 28.73950702861412 26.719815232073856 22.386091727999887 78-79 21.880580073763873 28.818459725544937 27.1514696782255 22.149490522465694 80-81 21.364113913046786 28.880937134360956 27.61081059978326 22.14413835280899 82-83 20.887977418778416 28.872659646986413 27.747635656093024 22.491727278142147 84-85 20.859468766901834 29.442256883369822 27.454502002898263 22.243772346830085 86-87 20.73563805075992 29.368439502036452 27.734760379991886 22.16116206721174 88-89 21.096705999522385 29.125477386876458 27.713982766493046 22.063833847108107 90-91 21.602622295968228 28.98491091165717 26.930964372438957 22.481502419935644 92-93 21.641754752429684 28.95122169888878 26.84659198992165 22.560431558759884 94-95 21.933626085977195 29.251532601931316 26.747308427032536 22.06753288505895 96-97 21.92755735574722 29.947652708271033 26.563670238526864 21.561119697454885 98-99 22.19372568873976 29.450778539413403 26.77015756095705 21.58533821088979 100-101 22.299212155411073 29.578575128204704 26.433716637404498 21.688496078979725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2281.0 1 1422.0 2 434.5 3 260.5 4 196.0 5 181.5 6 213.0 7 298.0 8 429.0 9 582.5 10 765.0 11 922.5 12 982.5 13 943.5 14 865.5 15 752.5 16 628.5 17 584.5 18 583.0 19 612.5 20 737.0 21 900.0 22 1060.5 23 1268.5 24 1644.0 25 2120.0 26 2701.5 27 3543.0 28 4434.0 29 5551.0 30 7115.0 31 9453.0 32 14045.5 33 22176.5 34 35479.5 35 54140.0 36 81394.0 37 113371.0 38 133933.0 39 137957.0 40 139987.0 41 143530.0 42 152205.0 43 167300.5 44 167746.0 45 160140.5 46 158732.5 47 155877.0 48 148007.5 49 133170.0 50 119985.0 51 117200.0 52 113358.5 53 102762.5 54 88839.0 55 73459.5 56 59455.0 57 45846.5 58 33181.5 59 24451.5 60 18630.5 61 14807.0 62 11729.5 63 9071.0 64 6957.5 65 5060.0 66 3667.0 67 2727.5 68 2142.0 69 1610.5 70 1162.0 71 916.5 72 699.5 73 530.0 74 401.0 75 310.0 76 258.5 77 202.5 78 153.0 79 119.5 80 95.5 81 83.5 82 70.0 83 58.0 84 47.5 85 35.0 86 34.5 87 28.0 88 20.0 89 16.0 90 9.5 91 11.0 92 15.0 93 8.5 94 6.0 95 6.0 96 2.0 97 1.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2210196513936801 2 0.23885817614438587 3 0.21798611298875417 4 0.21112767833413895 5 0.21910720326883548 6 0.24251820617641606 7 0.223888323580947 8 0.2104022669764393 9 0.20341193934769694 10-11 0.18928290449431903 12-13 0.2149196013402964 14-15 0.2120674157747954 16-17 0.21493608796206232 18-19 0.2031481533994425 20-21 0.1962237722577637 22-23 0.16506405712020925 24-25 0.49453270648999165 26-27 0.3501593596859892 28-29 0.48033772514955014 30-31 0.3145482566716412 32-33 0.36484893967940774 34-35 0.3669757138872091 36-37 0.3730922505623587 38-39 0.3664481419907002 40-41 0.37195467366051144 42-43 0.4260472796744354 44-45 0.38046177049171676 46-47 0.3993059791701426 48-49 0.3970308253664481 50-51 0.41172040535986665 52-53 0.4167323383767008 54-55 0.38161583401532995 56-57 0.3951348638633694 58-59 0.39187051275372087 60-61 0.35058801185190264 62-63 0.3136250058527507 64-65 0.33871764418045336 66-67 0.35042314563424365 68-69 0.3403827929788094 70-71 0.3132293269303691 72-73 0.30238112980840565 74-75 0.3143998770757481 76-77 0.3207142532120885 78-79 0.33001270788805714 80-81 0.337266821465054 82-83 0.3555175117599073 84-85 0.38380855471019487 86-87 0.3857045162132736 88-89 0.37907689426338104 90-91 0.3520718078108338 92-93 0.331216231276968 94-95 0.3096352433854025 96-97 0.3255778066330296 98-99 0.3546931806716122 100-101 0.37019060513155994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3032762.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.50563343958054 #Duplication Level Percentage of deduplicated Percentage of total 1 86.64827523510016 44.62874302430953 2 6.698296550781259 6.900000136282925 3 1.8828811186035606 2.9093695411530702 4 1.049212410760579 2.1616139931557194 5 0.6989399066234419 1.7999671313420822 6 0.5642108201596532 1.7436021411472915 7 0.32722400111595606 1.1797715617877929 8 0.2752557452263514 1.134177721261362 9 0.17865794877747962 0.8281701738720201 >10 1.3456732345336198 13.941881745270948 >50 0.19054885618873404 6.87458786491722 >100 0.1338983791006467 12.791175185442622 >500 0.0053326423322695386 1.862816678851352 >1k 0.0015931506962629544 1.2441231012062466 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4606 0.15187475970748776 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4078 0.13446488712269541 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012200100106767363 0.0 0.0 2 0.0013848762283357548 0.0 0.0 3 0.001450822715399362 0.0 0.0 4 0.0016156889330583804 0.0 0.0 5 0.0019454213683764172 0.0 0.0 6 0.002275153803694454 0.0 0.0 7 0.0027367792131397055 0.0 0.0 8 0.003033538404925939 0.0 0.0 9 0.0033962440837757794 0.0 0.0 10-11 0.005407611939215804 0.0 0.0 12-13 0.00743546641642173 1.648662176590184E-5 0.0 14-15 0.00949629413715946 3.297324353180368E-5 0.0 16-17 0.011161442935515548 3.297324353180368E-5 0.0 18-19 0.013321190386848689 3.297324353180368E-5 0.0 20-21 0.016074456221754296 3.297324353180368E-5 0.0 22-23 0.019190427735509743 3.297324353180368E-5 0.0 24-25 0.022322885871031095 3.297324353180368E-5 0.0 26-27 0.02573561657657277 4.945986529770552E-5 0.0 28-29 0.030368357292791193 6.594648706360736E-5 0.0 30-31 0.06231943027510896 6.594648706360736E-5 0.0 32-33 0.06932624452561724 6.594648706360736E-5 0.0 34-35 0.07625062566729601 6.594648706360736E-5 0.0 36-37 0.08376852519254727 6.594648706360736E-5 3.297324353180368E-5 38-39 0.09270427418966605 6.594648706360736E-5 3.297324353180368E-5 40-41 0.10229948805742092 6.594648706360736E-5 3.297324353180368E-5 42-43 0.11397201626767943 6.594648706360736E-5 3.297324353180368E-5 44-45 0.12780429192927106 6.594648706360736E-5 3.297324353180368E-5 46-47 0.1433017163892188 6.594648706360736E-5 3.297324353180368E-5 48-49 0.1602499635645659 6.594648706360736E-5 3.297324353180368E-5 50-51 0.17902822575592808 6.594648706360736E-5 3.297324353180368E-5 52-53 0.19746026889020635 6.594648706360736E-5 3.297324353180368E-5 54-55 0.22158019653372074 6.594648706360736E-5 3.297324353180368E-5 56-57 0.2489315020433519 6.594648706360736E-5 3.297324353180368E-5 58-59 0.27644767377064206 6.594648706360736E-5 3.297324353180368E-5 60-61 0.3070468437681559 6.594648706360736E-5 3.297324353180368E-5 62-63 0.339607921755812 6.594648706360736E-5 3.297324353180368E-5 64-65 0.37467496625188523 6.594648706360736E-5 3.297324353180368E-5 66-67 0.41430880497711325 6.594648706360736E-5 3.297324353180368E-5 68-69 0.4583940315791348 6.594648706360736E-5 3.297324353180368E-5 70-71 0.5047873852283826 6.594648706360736E-5 3.297324353180368E-5 72-73 0.5565883508168461 6.594648706360736E-5 3.297324353180368E-5 74-75 0.618248316221319 6.594648706360736E-5 3.297324353180368E-5 76-77 0.6906080991518622 6.594648706360736E-5 3.297324353180368E-5 78-79 0.7718047113489288 6.594648706360736E-5 3.297324353180368E-5 80-81 0.8579967699410636 6.594648706360736E-5 3.297324353180368E-5 82-83 0.9579716443294924 6.594648706360736E-5 3.297324353180368E-5 84-85 1.0741198946702708 6.594648706360736E-5 3.297324353180368E-5 86-87 1.2058150293362948 6.594648706360736E-5 3.297324353180368E-5 88-89 1.349743237352618 6.594648706360736E-5 3.297324353180368E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1015 0.0 64.463196 9 AGAGCGG 1170 0.0 59.166103 8 GATCGGG 2370 0.0 49.030598 1 GGATCGG 720 0.0 48.74709 1 ATCGGGA 2265 0.0 47.111607 2 CGGGAGA 1955 0.0 45.60478 4 TCGGGAG 2025 0.0 44.4967 3 TTGTGTT 26050 0.0 43.16913 1 GAGAGCG 2195 0.0 38.88222 7 TCGGAAA 890 0.0 35.168446 4 ACCGGAA 270 0.0 35.128975 3 GAATCGG 520 0.0 34.66009 1 GGCGCCG 1025 0.0 33.8342 56-57 CGCCGGA 550 0.0 32.82052 58-59 GATTGGG 2085 0.0 32.529655 1 TCGGGAA 755 0.0 32.034832 3 TGTGTTT 36655 0.0 30.767498 2 GAGGGGC 2145 0.0 30.724604 9 TCTCGGG 860 0.0 30.66539 48-49 ATTGGGA 1610 0.0 30.340656 2 >>END_MODULE