FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032762
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA46060.15187475970748776No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40780.13446488712269541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10150.064.4631969
AGAGCGG11700.059.1661038
GATCGGG23700.049.0305981
GGATCGG7200.048.747091
ATCGGGA22650.047.1116072
CGGGAGA19550.045.604784
TCGGGAG20250.044.49673
TTGTGTT260500.043.169131
GAGAGCG21950.038.882227
TCGGAAA8900.035.1684464
ACCGGAA2700.035.1289753
GAATCGG5200.034.660091
GGCGCCG10250.033.834256-57
CGCCGGA5500.032.8205258-59
GATTGGG20850.032.5296551
TCGGGAA7550.032.0348323
TGTGTTT366550.030.7674982
GAGGGGC21450.030.7246049
TCTCGGG8600.030.6653948-49
ATTGGGA16100.030.3406562
Produced by FastQC (version 0.11.2)