FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596973
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA297951.1472972572298596No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC232630.8957736564839142No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT116310.44786757505757663No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC115400.44436349550033827No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT108980.4196424067558654No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT65950.25394950197787963Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT56770.21860065545540905No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC50540.19461118771739253No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT47860.18429148088948172No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC34910.13442573334416646No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32690.12587731947925526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC28200.086.4129269
ATCGGGA44550.085.906232
CGGGAGA39350.083.999854
AAGCGGC8800.080.811539
AGAGCGG32050.080.029618
TCGGGAG43050.079.644463
TCGGAGA11550.078.4227144
ATTCGGA7200.078.3814542
CGGAGAG18950.076.327515
ATCCGGA8500.075.878522
GTTCGGA6000.075.878522
TTGGGAG24000.072.91313
GTCCGGA3650.072.7602162
CCGGAGA9550.071.507174
AAGCGTC3800.071.114149
CGGAAAG16650.070.636295
AAGGGGC24350.067.950279
AATCGGA6150.067.858842
GAGAGCG44800.065.8287
GATCCGG10400.064.7570951