##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_30245-1.3420000003577e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180830 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899891325779635 33.0 33.0 33.0 33.0 33.0 2 31.81076746009547 33.0 33.0 33.0 33.0 33.0 3 31.772949748490255 33.0 33.0 33.0 33.0 33.0 4 35.45284914459174 37.0 37.0 37.0 33.0 37.0 5 35.44179693052645 37.0 37.0 37.0 33.0 37.0 6 35.503852661601314 37.0 37.0 37.0 33.0 37.0 7 35.42487768418446 37.0 37.0 37.0 33.0 37.0 8 35.403345973780624 37.0 37.0 37.0 33.0 37.0 9 35.267811337885114 37.0 37.0 37.0 33.0 37.0 10-11 35.22994410385037 37.0 37.0 37.0 33.0 37.0 12-13 35.26081102149182 37.0 37.0 37.0 33.0 37.0 14-15 37.045059220571986 40.0 37.0 40.0 33.0 40.0 16-17 36.99727626637565 40.0 37.0 40.0 33.0 40.0 18-19 37.01496815432657 40.0 37.0 40.0 33.0 40.0 20-21 36.98142679621979 40.0 37.0 40.0 33.0 40.0 22-23 37.12021890748018 40.0 37.0 40.0 33.0 40.0 24-25 36.8726058427296 40.0 37.0 40.0 33.0 40.0 26-27 36.77360500359955 40.0 37.0 40.0 33.0 40.0 28-29 36.63786929746931 40.0 37.0 40.0 33.0 40.0 30-31 36.69106166001018 40.0 37.0 40.0 33.0 40.0 32-33 36.637701929999125 40.0 37.0 40.0 33.0 40.0 34-35 36.537515533076856 40.0 37.0 40.0 33.0 40.0 36-37 36.46065007359583 40.0 37.0 40.0 33.0 40.0 38-39 36.448239661046486 40.0 37.0 40.0 33.0 40.0 40-41 36.30665113741099 40.0 37.0 40.0 33.0 40.0 42-43 36.21613697537177 40.0 37.0 40.0 33.0 40.0 44-45 36.14255673298698 40.0 37.0 40.0 33.0 40.0 46-47 36.056103639440025 40.0 37.0 40.0 30.0 40.0 48-49 35.94463827992095 38.5 37.0 40.0 27.0 40.0 50-51 34.84250675201643 37.0 35.0 38.5 27.0 38.5 52-53 35.263471017915194 37.0 37.0 38.5 27.0 40.0 54-55 36.05053488809307 38.5 37.0 40.0 33.0 40.0 56-57 36.12163671629609 40.0 37.0 40.0 30.0 40.0 58-59 35.95616921080506 37.0 37.0 40.0 27.0 40.0 60-61 35.754685830624126 37.0 37.0 40.0 27.0 40.0 62-63 35.55008574716965 37.0 37.0 40.0 27.0 40.0 64-65 35.32672101906155 37.0 37.0 40.0 27.0 40.0 66-67 35.10163424017462 37.0 37.0 40.0 27.0 40.0 68-69 34.923225560910296 37.0 35.0 40.0 27.0 40.0 70-71 34.64367511452062 37.0 33.0 37.0 27.0 40.0 72-73 34.44247396633392 37.0 33.0 37.0 27.0 40.0 74-75 34.212203610551946 37.0 33.0 37.0 27.0 40.0 76-77 33.96658084307351 37.0 33.0 37.0 27.0 38.5 78-79 33.697937253247616 37.0 33.0 37.0 27.0 37.0 80-81 33.43784797531215 37.0 33.0 37.0 27.0 37.0 82-83 33.208389007854805 37.0 33.0 37.0 27.0 37.0 84-85 33.016120238624744 37.0 33.0 37.0 27.0 37.0 86-87 32.793491239573925 37.0 33.0 37.0 24.5 37.0 88-89 32.657161722830296 37.0 33.0 37.0 22.0 37.0 90-91 32.53214991539918 37.0 33.0 37.0 22.0 37.0 92-93 32.37006713040448 35.0 33.0 37.0 22.0 37.0 94-95 32.24549116620736 33.0 33.0 37.0 22.0 37.0 96-97 32.08208617819821 33.0 33.0 37.0 22.0 37.0 98-99 31.962720157004444 33.0 33.0 37.0 22.0 37.0 100-101 30.714756996189525 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.08914517477981931 2306 2 0.00415805314410278 2306 3 -0.11434802999145433 2306 4 0.02896454407083837 2306 5 0.07410775825567129 2306 6 -0.01832836973809293 2306 7 0.03611524747049799 2306 8 -0.09554012305385129 2306 9 0.08414928115760745 2306 10-11 0.10834316619668982 2306 12-13 -0.030713400556976467 2306 14-15 0.27261178449960966 2306 16-17 0.3638279714785497 2306 18-19 0.20367975536331784 2306 20-21 0.2799757114680119 2306 22-23 0.3054622896556012 2306 24-25 0.3578208217726484 2306 26-27 0.2626952926301698 2306 28-29 0.08336781318793385 2306 30-31 0.42849781844547863 2306 32-33 0.4390289377203729 2306 34-35 0.4934962490314234 2306 36-37 0.5564898343349256 2306 38-39 0.21122447056670524 2306 40-41 0.32093427017095166 2306 42-43 0.2690912388853235 2306 44-45 0.40377633140226266 2306 46-47 0.2580184782468322 2306 48-49 0.4538510081047349 2306 50-51 0.49484542089921746 2306 52-53 0.5043112519755582 2306 54-55 0.629822391121337 2306 56-57 0.500105458486324 2306 58-59 0.4279555311376342 2306 60-61 0.38231305851279274 2306 62-63 0.44752403718860734 2306 64-65 0.40759591765668546 2306 66-67 0.29489782892159866 2306 68-69 0.3366163547037573 2306 70-71 0.46511244120279116 2306 72-73 0.4709633864938567 2306 74-75 0.44942400418779727 2306 76-77 0.40744967666618237 2306 78-79 0.3979126892865139 2306 80-81 0.17676048602184125 2306 82-83 0.26247895000991406 2306 84-85 0.3470499750124958 2306 86-87 0.39579326883627886 2306 88-89 0.3260763168416645 2306 90-91 0.30955431456273175 2306 92-93 0.16616077346300528 2306 94-95 0.38080861199902216 2306 96-97 0.3336356660085116 2306 98-99 0.16308561916445896 2306 100-101 0.11416255645701057 2307 1 -0.11810970590991232 2307 2 -0.15549941416476187 2307 3 -0.2890890042648806 2307 4 -0.10869677992831583 2307 5 -0.1056240662168193 2307 6 -0.16281815628646967 2307 7 -0.09192730730259058 2307 8 -0.16110385792170234 2307 9 -0.12181251924337033 2307 10-11 -0.11320554419516071 2307 12-13 -0.19209998347253077 2307 14-15 -0.037014418256482884 2307 16-17 -0.010370464419274583 2307 18-19 0.04239511019412845 2307 20-21 0.015790787104180026 2307 22-23 -0.045737569544598955 2307 24-25 -0.20473889242998666 2307 26-27 -0.15348127119870725 2307 28-29 -0.19264872204371386 2307 30-31 0.060729386023922416 2307 32-33 0.047528687385323565 2307 34-35 0.0638155300778891 2307 36-37 0.1921173226544468 2307 38-39 0.002109705608361878 2307 40-41 0.11125160538161794 2307 42-43 0.19785342495532632 2307 44-45 0.07395361016181567 2307 46-47 0.1962917566740714 2307 48-49 0.013486502773361053 2307 50-51 -0.08123320617151109 2307 52-53 -0.12124981556680581 2307 54-55 -0.029588567213721717 2307 56-57 -0.04763939434071318 2307 58-59 0.19901346457255897 2307 60-61 0.3049248183572928 2307 62-63 0.12838428245137123 2307 64-65 0.027576645136910827 2307 66-67 0.03834921691470328 2307 68-69 -0.11131067426733665 2307 70-71 0.05615678658208623 2307 72-73 -0.13923924278943645 2307 74-75 -0.003572582947384717 2307 76-77 -0.03141281833258347 2307 78-79 -0.05491117759208208 2307 80-81 -0.047905661026497626 2307 82-83 -0.035251418365227494 2307 84-85 -0.06556805960589429 2307 86-87 -0.1663923092409263 2307 88-89 -0.19324020286703103 2307 90-91 -0.056662368464991175 2307 92-93 -0.24764034025469783 2307 94-95 -0.3044169191157664 2307 96-97 -0.0894412360459853 2307 98-99 -0.19113793769220067 2307 100-101 -0.5563052312151378 2201 1 0.04437672377363455 2201 2 -0.08040678830369785 2201 3 -0.04505994350463283 2201 4 -0.0990671674695065 2201 5 0.008501812231777706 2201 6 -0.010982251836949786 2201 7 0.023573954109032513 2201 8 -0.012108544268706112 2201 9 0.036428960915962705 2201 10-11 -0.06399696891418927 2201 12-13 -0.08926934102187545 2201 14-15 -0.026148506505066393 2201 16-17 0.1541355760617975 2201 18-19 0.18754823745182136 2201 20-21 0.15870312189402114 2201 22-23 0.12940644000581614 2201 24-25 0.054400063747067406 2201 26-27 -0.01808718148201649 2201 28-29 0.16260043089809528 2201 30-31 0.2193514145592559 2201 32-33 0.08883508871124945 2201 34-35 0.1900498976528482 2201 36-37 0.1283397328971887 2201 38-39 0.06171140474720005 2201 40-41 0.05490485047408811 2201 42-43 0.12297135631752809 2201 44-45 0.13647027122650712 2201 46-47 0.2869318119984712 2201 48-49 0.3134629358180945 2201 50-51 0.059274623080099786 2201 52-53 0.2887205518845235 2201 54-55 0.2781446780332857 2201 56-57 0.4201226528163744 2201 58-59 0.35251595842003525 2201 60-61 0.3440493446035475 2201 62-63 0.4396762345293226 2201 64-65 0.3223128111409963 2201 66-67 0.25941498417363107 2201 68-69 0.31825986626589753 2201 70-71 0.20639226296297153 2201 72-73 0.12370809472412958 2201 74-75 0.21096880796659434 2201 76-77 0.16725468283610923 2201 78-79 0.21799609609754356 2201 80-81 0.03395425467333979 2201 82-83 0.28881836300297437 2201 84-85 0.339294363463452 2201 86-87 0.3223472256787332 2201 88-89 0.37074359774880605 2201 90-91 0.17650850741443236 2201 92-93 0.088609886722125 2201 94-95 0.150144252586891 2201 96-97 0.2574387502001869 2201 98-99 0.16839335033848712 2201 100-101 0.03807076603409598 1101 1 0.17450757376588655 1101 2 0.24450507439582125 1101 3 0.14596312716017223 1101 4 0.35821771940481284 1101 5 0.4280305198096528 1101 6 0.3828946271340712 1101 7 0.40803902849303597 1101 8 0.26451909016488884 1101 9 0.4328870036867656 1101 10-11 0.3680162419954627 1101 12-13 0.3647636668814229 1101 14-15 0.4126263365911669 1101 16-17 0.3959332380019802 1101 18-19 0.43148758736658976 1101 20-21 0.4504605893510245 1101 22-23 0.2749540535189112 1101 24-25 0.17096164363238842 1101 26-27 0.332337281945847 1101 28-29 0.3316945053595859 1101 30-31 0.45356660516827674 1101 32-33 0.4124430520616258 1101 34-35 0.4184623124211768 1101 36-37 0.48869174940114135 1101 38-39 0.4065570676171859 1101 40-41 0.44432764801011615 1101 42-43 0.5603054935405254 1101 44-45 0.43232830237480613 1101 46-47 0.37825319352643305 1101 48-49 0.3817039671213678 1101 50-51 0.5085436161840846 1101 52-53 0.5078652326531596 1101 54-55 0.6050057310318948 1101 56-57 0.5854103581472287 1101 58-59 0.6924850135106979 1101 60-61 0.6742131250157541 1101 62-63 0.511514187806867 1101 64-65 0.6176187734840894 1101 66-67 0.7579464327246939 1101 68-69 0.6748478873413646 1101 70-71 0.6570075291182533 1101 72-73 0.5917861688756929 1101 74-75 0.613136404433348 1101 76-77 0.5392739955588723 1101 78-79 0.5550353123535459 1101 80-81 0.4966000202087528 1101 82-83 0.5921002667686608 1101 84-85 0.5889081574352559 1101 86-87 0.5554764388354059 1101 88-89 0.5546309369708737 1101 90-91 0.5501525489454977 1101 92-93 0.5175261853429234 1101 94-95 0.6099485587235662 1101 96-97 0.6645543844013346 1101 98-99 0.5802003587066871 1101 100-101 0.5220401891706246 2304 1 -0.024354779440979968 2304 2 -4.7572957888775136E-4 2304 3 -0.051149000211101026 2304 4 0.010687796188918242 2304 5 0.07344041140856916 2304 6 -0.051534008818954646 2304 7 0.1365197195499448 2304 8 -0.016733732780004118 2304 9 0.16928605517728101 2304 10-11 0.12703272799365095 2304 12-13 0.08976366688142434 2304 14-15 0.06849947344753815 2304 16-17 0.08767471270839877 2304 18-19 0.015241539489451839 2304 20-21 0.047771734337473504 2304 22-23 0.0033811086227899523 2304 24-25 -0.09656602122758784 2304 26-27 -0.004901595651261914 2304 28-29 0.06367423661522764 2304 30-31 0.12286707942301689 2304 32-33 0.16240211936064242 2304 34-35 0.20530315478793426 2304 36-37 0.1924307399160483 2304 38-39 0.13205531739134813 2304 40-41 0.20587490410768083 2304 42-43 0.13106401883411678 2304 44-45 0.2144844676864608 2304 46-47 0.16571649524279053 2304 48-49 0.06136464914485629 2304 50-51 0.019402073726993763 2304 52-53 0.26515125343003376 2304 54-55 0.41178926761725876 2304 56-57 0.3594293847958241 2304 58-59 0.4843168224537848 2304 60-61 0.44812629123075 2304 62-63 0.3316657799477909 2304 64-65 0.19913130735942985 2304 66-67 0.11312794808151949 2304 68-69 0.14233885391769974 2304 70-71 0.1378637283052413 2304 72-73 0.1938144547835421 2304 74-75 0.32631391120841613 2304 76-77 0.31516886909093955 2304 78-79 0.19324810593981567 2304 80-81 0.1906181999738763 2304 82-83 0.20357553777137838 2304 84-85 0.30222822292757456 2304 86-87 0.2554044537405531 2304 88-89 0.3199651736465725 2304 90-91 0.13508931497982957 2304 92-93 0.2060283307810451 2304 94-95 0.11476309124389417 2304 96-97 0.22379529451876579 2304 98-99 0.24841682212132454 2304 100-101 0.20435020497911083 2202 1 -0.06994577381246359 2202 2 -0.18737170623095878 2202 3 -0.09824093266888667 2202 4 -0.13932679626469735 2202 5 -0.1076852921561624 2202 6 -0.16583044409100012 2202 7 -0.037885116806108954 2202 8 -0.1485702972995 2202 9 -0.11415359460383456 2202 10-11 -0.13997164974242793 2202 12-13 -0.09109103397328511 2202 14-15 -0.0021092403319116215 2202 16-17 -0.004382823251582124 2202 18-19 -0.10435821035420645 2202 20-21 0.03011069618862905 2202 22-23 -0.11882443650958407 2202 24-25 -0.06167546462971529 2202 26-27 -0.0482245058034394 2202 28-29 -0.07742853595095767 2202 30-31 -0.018233608506939447 2202 32-33 -0.0393990063429257 2202 34-35 0.1330892711106273 2202 36-37 0.12038779640968755 2202 38-39 -0.005543181670560671 2202 40-41 0.07958138732635689 2202 42-43 0.03414719581972747 2202 44-45 0.07983364425515305 2202 46-47 0.058764233412475164 2202 48-49 -0.05851861122620505 2202 50-51 0.09455946376242963 2202 52-53 -0.02356461065168247 2202 54-55 0.041976884878046405 2202 56-57 0.14835996640933757 2202 58-59 -0.021449281361100248 2202 60-61 0.0867754469530837 2202 62-63 0.025015790370964908 2202 64-65 0.1560001837405025 2202 66-67 0.1811079355736993 2202 68-69 0.05032509531856988 2202 70-71 0.09469716587039301 2202 72-73 0.12847153212354812 2202 74-75 0.02273042152737048 2202 76-77 -0.0016145372046594275 2202 78-79 0.07146782659855688 2202 80-81 0.15697217120590068 2202 82-83 0.025706035999434107 2202 84-85 0.18331431840391588 2202 86-87 0.2398968448183112 2202 88-89 0.1760981734381062 2202 90-91 0.0522750560680052 2202 92-93 0.11458993178166565 2202 94-95 0.14074182795433643 2202 96-97 0.06207130035574693 2202 98-99 0.18463946127078756 2202 100-101 0.07761266068059669 1102 1 -0.06524512931840931 1102 2 -0.06234566160826205 1102 3 -0.07011652387551592 1102 4 0.05739170739920496 1102 5 0.01519815658070911 1102 6 -0.09388321440643921 1102 7 0.05134035521326297 1102 8 -0.05861078312945267 1102 9 0.06707737642546618 1102 10-11 0.10165711663793786 1102 12-13 -0.035185778382825106 1102 14-15 -0.035668209645919546 1102 16-17 0.0468563458429756 1102 18-19 0.10180950944853606 1102 20-21 0.02700703480655875 1102 22-23 -0.08858971959507755 1102 24-25 0.0011145261632279357 1102 26-27 0.04844354822811425 1102 28-29 0.08171571216033158 1102 30-31 0.13767038584269642 1102 32-33 0.06865704319861266 1102 34-35 0.31121695913010683 1102 36-37 0.07513308882836611 1102 38-39 0.1535290495905457 1102 40-41 0.049393966942183454 1102 42-43 0.06058545177593544 1102 44-45 0.08353563827687083 1102 46-47 -0.09558956985918599 1102 48-49 0.021214257970505912 1102 50-51 0.16396768957498864 1102 52-53 0.18186717513918182 1102 54-55 0.3120248120287741 1102 56-57 0.30634551258966525 1102 58-59 0.40879224625376054 1102 60-61 0.3912213208037727 1102 62-63 0.38715081881195346 1102 64-65 0.33966080906369456 1102 66-67 0.34219108918021135 1102 68-69 0.2645404143494332 1102 70-71 0.2905006393275755 1102 72-73 0.22619814556585993 1102 74-75 0.26488600819352826 1102 76-77 0.10829515795343525 1102 78-79 0.20189753134209099 1102 80-81 0.12823732186111414 1102 82-83 0.2861817760324783 1102 84-85 0.18835083132532304 1102 86-87 0.0733460861819637 1102 88-89 0.14400123446954893 1102 90-91 -1.5396760548469501E-4 1102 92-93 0.15146520767809335 1102 94-95 0.1687171580841138 1102 96-97 0.2963996265630655 1102 98-99 0.07118504881982446 1102 100-101 0.01767799295448569 2305 1 -0.003938447805136747 2305 2 -0.039166578025831456 2305 3 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60-61 -1.0520155213783653 1213 62-63 -0.8425165314283092 1213 64-65 -0.6689274779486496 1213 66-67 -0.5299403888225456 1213 68-69 -0.5642582279956088 1213 70-71 -0.6988640431612794 1213 72-73 -0.5115173265742783 1213 74-75 -0.46574458268067787 1213 76-77 -0.578475655663155 1213 78-79 -0.5174009705415727 1213 80-81 -0.44832435575364826 1213 82-83 -0.5836789480901459 1213 84-85 -0.6555216159024866 1213 86-87 -0.6462649365075848 1213 88-89 -0.5670636319337703 1213 90-91 -0.5555416494729926 1213 92-93 -0.5947287655676661 1213 94-95 -0.4924589318985042 1213 96-97 -0.38461019466272717 1213 98-99 -0.4722916309775904 1213 100-101 -0.2928085446640232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2314.0 3 240.0 4 196.0 5 178.0 6 431.0 7 2215.0 8 9057.0 9 13884.0 10 14891.0 11 11859.0 12 8708.0 13 6313.0 14 5044.0 15 4374.0 16 4171.0 17 4209.0 18 4481.0 19 5014.0 20 5673.0 21 6614.0 22 7415.0 23 8052.0 24 9174.0 25 10615.0 26 12587.0 27 15263.0 28 18526.0 29 22670.0 30 28459.0 31 37106.0 32 50318.0 33 72916.0 34 111323.0 35 189541.0 36 378351.0 37 749744.0 38 347698.0 39 1206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.35667018115066 24.909295978688807 26.193400185130884 14.54063365502965 2 29.688814438818806 23.412973864531722 34.67084498275686 12.227366713892609 3 38.102935343573584 20.108944187245754 26.530189222963767 15.257931246216897 4 28.310263896465344 19.615325048587593 36.375275911813 15.699135143134063 5 30.8604769805632 18.394821143778984 35.139605748769405 15.605096126888412 6 23.778322084030957 18.926319138157247 41.7695745676581 15.5257842101537 7 32.012750036770115 18.814669436681868 32.136895131637004 17.035685394911017 8 27.932827391100943 28.09238577467158 27.839031693846433 16.135755140381047 9 30.34308769736963 26.868968623053874 25.619864713343016 17.16807896623348 10-11 24.30950357679525 29.167462109908314 29.921806493527676 16.601227819768756 12-13 24.986574301846463 26.15070929278914 29.973672533376966 18.889043871987433 14-15 23.331976838638177 26.91856872729011 30.933547921449534 18.81590651262218 16-17 22.463184478022136 28.13083886652887 31.9712116807778 17.434764974671197 18-19 21.9429025968446 28.308184074469438 32.1647586254751 17.584154703210867 20-21 21.746560235848538 28.98943258135782 31.698120490076793 17.56588669271685 22-23 22.164395007164416 28.82138663287216 31.25551914847391 17.75869921148952 24-25 21.720448641962108 29.163294539273686 31.220399788362517 17.89585703040169 26-27 21.127476787594524 29.86482593457084 31.076781335826997 17.930915942007644 28-29 21.203004781633073 29.484609860168394 30.93294723291758 18.379438125280952 30-31 21.492008698128302 29.204007174529778 30.848706942355808 18.455277184986112 32-33 20.496773768916338 29.281240176208044 32.182869009817004 18.03911704505861 34-35 20.224691691580883 30.160660750158275 31.77235427339737 17.84229328486347 36-37 20.334507508236676 30.41845660251859 31.33910025443653 17.907935634808204 38-39 20.618360489735803 29.453811101905554 31.70641167265028 18.221416735708367 40-41 20.94202434911974 29.611283136653437 30.773940025252234 18.672752488974588 42-43 20.871754897230975 29.236350908058828 31.412928712805165 18.47896548190503 44-45 20.397587169564474 29.54117599129219 31.635887895038607 18.425348944104726 46-47 20.833567385336774 29.737527176890826 30.944199546752536 18.48470589101986 48-49 20.46761614434344 30.124092339950025 30.581795646362615 18.826495869343916 50-51 20.38896044361577 29.532111904122704 30.830135627546213 19.248792024715314 52-53 21.09327563391571 29.260595348755814 30.370136360695348 19.275992656633125 54-55 21.485558951884656 29.283955107076437 30.154100760491282 19.07638518054763 56-57 21.70783868212718 29.30227418697643 29.986717219501973 19.003169911394416 58-59 21.37898187629213 29.265606666898396 30.52989936440068 18.82551209240879 60-61 20.472318287348624 29.524966578539857 30.371605219500186 19.631109914611333 62-63 20.88792807811548 29.48786685057928 29.958435657150638 19.665769414154603 64-65 20.38118201395152 29.49503966428006 30.36362757909849 19.76015074266993 66-67 20.19317166511849 29.11521201552055 30.45003208713158 20.24158423222938 68-69 19.84369291310322 29.38029340766843 31.21526470834549 19.560748970882862 70-71 19.509534929668234 30.678895824530887 30.51253395146134 19.299035294339532 72-73 19.49417328542438 31.111288960519275 29.893373147069806 19.50116460698654 74-75 19.893883585674928 30.64886590037257 29.915117908915978 19.542132605036528 76-77 19.928091196427516 30.551028637240535 30.110310616496882 19.410569549835063 78-79 19.833185030867465 30.649058701091242 30.17311820734065 19.344638060700646 80-81 19.424227386285807 31.129576215951083 30.03975312067404 19.406443277089075 82-83 19.264294392695408 30.820684989507015 30.00066731710909 19.914353300688486 84-85 19.373191502388927 30.36680837331373 30.46171169875111 19.798288425546236 86-87 19.546324747527876 30.468073293509878 30.648307764054433 19.337294194907813 88-89 19.835470113503572 30.971309119943953 29.882468459274126 19.310752307278356 90-91 20.034087081056107 30.574160455488446 29.675986348600347 19.7157661148551 92-93 19.60611461181868 30.851257413618306 29.93996409854473 19.602663876018283 94-95 19.236841966056055 31.119223778287292 29.997127370637955 19.646806885018698 96-97 19.554477366972694 31.4438528260316 29.62484665448567 19.376823152510035 98-99 20.014966163905726 30.7757162485276 29.287774894536177 19.921542693030496 100-101 20.010522248272125 30.64733690687787 28.966078637465507 20.37606220738449 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1741.0 1 1094.0 2 367.0 3 265.5 4 275.5 5 310.0 6 376.5 7 510.5 8 655.5 9 805.0 10 919.5 11 983.0 12 966.5 13 881.5 14 823.0 15 787.0 16 813.5 17 894.0 18 953.0 19 1121.5 20 1452.5 21 1932.0 22 2707.0 23 3905.5 24 5453.0 25 7669.0 26 11461.5 27 16415.5 28 21785.0 29 27437.0 30 33199.5 31 38799.0 32 45413.5 33 54652.0 34 66467.5 35 82000.5 36 101222.0 37 120684.5 38 140118.5 39 151656.5 40 152253.0 41 141363.0 42 124917.5 43 114737.5 44 104315.0 45 94929.0 46 91464.0 47 83331.0 48 66892.5 49 53487.0 50 44225.5 51 35576.5 52 28464.5 53 23017.5 54 18671.5 55 14669.0 56 10899.0 57 7705.0 58 5605.0 59 4178.5 60 3020.5 61 2147.0 62 1538.0 63 1054.0 64 743.5 65 528.5 66 401.5 67 322.0 68 242.5 69 186.0 70 137.0 71 106.5 72 93.0 73 73.0 74 63.0 75 56.5 76 44.5 77 34.0 78 29.5 79 26.0 80 25.0 81 26.0 82 23.0 83 20.0 84 16.0 85 12.0 86 7.0 87 7.0 88 8.0 89 6.0 90 5.0 91 5.0 92 2.5 93 1.5 94 3.0 95 3.5 96 2.0 97 2.5 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23257200240275494 2 0.25091364297079555 3 0.23096710885305138 4 0.22298849520595368 5 0.23202175318571372 6 0.2560493023298469 7 0.23614862231352285 8 0.22120018525056975 9 0.21597281768867818 10-11 0.2004053502565537 12-13 0.2276885406015141 14-15 0.22369923377796525 16-17 0.22686316677595228 18-19 0.21500988155885603 20-21 0.20716883021601865 22-23 0.17197580737609075 24-25 0.508155151937565 26-27 0.36013811255347733 28-29 0.49318378782390193 30-31 0.32230847888189357 32-33 0.3746738627036495 34-35 0.3763933915069033 36-37 0.384372005154001 38-39 0.3773792546874355 40-41 0.3828358927564276 42-43 0.43916765635102234 44-45 0.39097499575849565 46-47 0.41030249950706843 48-49 0.4080556485374834 50-51 0.4228206691947561 52-53 0.4285753589229789 54-55 0.39205256714186804 56-57 0.405579527060798 58-59 0.4028512080263019 60-61 0.3588083436122944 62-63 0.31967186805023773 64-65 0.3480326297785706 66-67 0.35951908218430595 68-69 0.3488580036041324 70-71 0.31889234832609603 72-73 0.3074976041232008 74-75 0.32056602302792975 76-77 0.32577046353911127 78-79 0.3367525208292256 80-81 0.34580870585969564 82-83 0.3645401062898071 84-85 0.39523942719056504 86-87 0.3970735912473692 88-89 0.39065401704855496 90-91 0.36068836177051855 92-93 0.3384261955310593 94-95 0.3147196250968668 96-97 0.3313188098109435 98-99 0.3635542431092749 100-101 0.38015342782335165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180830.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.7500733836874 #Duplication Level Percentage of deduplicated Percentage of total 1 92.812331076309 63.80854572407334 2 3.571384707029495 4.910659213793133 3 1.1121187494341853 2.293747369049247 4 0.5932311521210606 1.6313874096724932 5 0.37138542558435395 1.2766387631278155 6 0.2608251692496384 1.0759049715735194 7 0.17920053268488695 0.8624034840737295 8 0.15928825681118508 0.8760863475942895 9 0.12437521456279772 0.7695724615473788 >10 0.6906599325557161 9.075530991933247 >50 0.06242407608356488 2.9973473139335396 >100 0.05938276561299211 8.227996691576598 >500 0.0025824597496504984 1.141101402643354 >1k 8.104822116149541E-4 1.0530778554082987 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2719 0.12467730176125603 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2535 0.11624014709995735 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2503 0.1147728158545141 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2377 0.10899519907558132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.795197241417259E-4 0.0 0.0 2 7.795197241417259E-4 0.0 0.0 3 9.62936129822132E-4 0.0 0.0 4 0.001054644332662335 0.0 0.0 5 0.0012380607383427411 0.0 0.0 6 0.0014214771440231471 0.0 0.0 7 0.0016966017525437563 0.0 0.0 8 0.001925872259644264 0.0 0.0 9 0.00210928866532467 0.0 0.0 10-11 0.003255641200827208 0.0 0.0 12-13 0.004585410142010152 0.0 0.0 14-15 0.006006887286033299 0.0 0.0 16-17 0.007061531618695634 0.0 0.0 18-19 0.00818495710348812 0.0 0.0 20-21 0.00995034000816203 0.0 0.0 22-23 0.01203670162277665 0.0 4.585410142010152E-5 24-25 0.014123063237391268 0.0 4.585410142010152E-5 26-27 0.016736747018337054 0.0 4.585410142010152E-5 28-29 0.019465066052833095 0.0 4.585410142010152E-5 30-31 0.030011509379456444 0.0 4.585410142010152E-5 32-33 0.0333817858338339 0.0 4.585410142010152E-5 34-35 0.036912551643181725 0.0 4.585410142010152E-5 36-37 0.04053502565536975 0.0 4.585410142010152E-5 38-39 0.04424920787039797 0.0 4.585410142010152E-5 40-41 0.048742909809567914 0.0 4.585410142010152E-5 42-43 0.05383271506719919 0.0 4.585410142010152E-5 44-45 0.05906008262909076 0.0 4.585410142010152E-5 46-47 0.0649981887629939 0.0 4.585410142010152E-5 48-49 0.07148654411393827 0.0 4.585410142010152E-5 50-51 0.0782729511241133 0.0 4.585410142010152E-5 52-53 0.08540326389493907 0.0 4.585410142010152E-5 54-55 0.09324431523777643 0.0 6.878115213015228E-5 56-57 0.10138341823984445 0.0 9.170820284020305E-5 58-59 0.10963715649546274 0.0 9.170820284020305E-5 60-61 0.11853285217096243 0.0 9.170820284020305E-5 62-63 0.1295836906132069 0.0 1.1463525355025381E-4 64-65 0.1417350274895338 0.0 1.3756230426030457E-4 66-67 0.15601858008189542 0.0 1.3756230426030457E-4 68-69 0.1697518834572158 0.0 1.3756230426030457E-4 70-71 0.18651155752626294 0.0 1.3756230426030457E-4 72-73 0.2038444078630613 0.0 1.3756230426030457E-4 74-75 0.22278215174956323 0.0 1.3756230426030457E-4 76-77 0.24575505656103408 0.0 1.3756230426030457E-4 78-79 0.2719606755226221 0.0 1.3756230426030457E-4 80-81 0.3005736348087655 0.0 1.3756230426030457E-4 82-83 0.3335656607805285 0.0 1.3756230426030457E-4 84-85 0.37018016076447957 0.0 1.6048935497035534E-4 86-87 0.41057762411558907 0.0 1.834164056804061E-4 88-89 0.4583575977953348 0.0 1.834164056804061E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 780 0.0 74.199265 2 GAGCGGC 600 0.0 72.72122 9 CGGGAGA 795 0.0 65.038994 4 GAGAGCG 790 0.0 61.237526 7 AGAGCGG 715 0.0 61.027603 8 GATCGGG 1035 0.0 58.21671 1 TCGGGAG 945 0.0 54.213364 3 GATCCGG 220 0.0 53.914005 1 GGTTCGG 235 0.0 52.491596 1 TTCGGAG 245 0.0 52.277172 3 GTTCGGA 160 0.0 50.403805 2 TTGTGTT 28030 0.0 48.74765 1 CGCGTTA 20 5.400237E-4 47.48144 60-61 ATTCGGA 220 0.0 47.438873 2 AAGCGGC 190 0.0 47.426884 9 TCGGAGA 370 0.0 46.15462 4 CGGAAAG 395 0.0 44.434372 5 GTCGGGA 185 0.0 43.59248 2 TCGGGAA 360 0.0 43.483635 3 TCGTGCG 45 9.6881716E-4 42.16789 2 >>END_MODULE