FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180830
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG27190.12467730176125603No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC25350.11624014709995735No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25030.1147728158545141No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23770.10899519907558132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA7800.074.1992652
GAGCGGC6000.072.721229
CGGGAGA7950.065.0389944
GAGAGCG7900.061.2375267
AGAGCGG7150.061.0276038
GATCGGG10350.058.216711
TCGGGAG9450.054.2133643
GATCCGG2200.053.9140051
GGTTCGG2350.052.4915961
TTCGGAG2450.052.2771723
GTTCGGA1600.050.4038052
TTGTGTT280300.048.747651
CGCGTTA205.400237E-447.4814460-61
ATTCGGA2200.047.4388732
AAGCGGC1900.047.4268849
TCGGAGA3700.046.154624
CGGAAAG3950.044.4343725
GTCGGGA1850.043.592482
TCGGGAA3600.043.4836353
TCGTGCG459.6881716E-442.167892
Produced by FastQC (version 0.11.2)