FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184150
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA314581.440285694663828No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC216450.9910033651534922No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT120180.550236934276492No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT114370.5236361971476318No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC102580.46965638806858506No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT58510.2678845317400362No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT54670.250303321658311No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51460.2356065288556189No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC30970.14179429068516355No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT28360.129844561957741No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC27520.12599867225236364No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26880.12306847057207611No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT25760.11794061763157292Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA24440.1118970766659799No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT22890.10480049447153354No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG22670.10379323764393471No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA22350.10232813680379094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9050.066.5582669
AAGCGGC3400.065.564059
AGAGCGG9850.063.0785838
GTTCGGA2550.057.6631362
CGGGAGA13250.057.635064
GGTTCGG3300.057.5045971
TCGGAGA4950.057.4940384
ATTCGGA2400.057.314372
TCGGGAG13250.054.4132233
GGATCGG7150.053.744681
ATCGGGA16650.052.4179882
TTGTGTT291050.052.1438521
TCGGAAA4550.051.0812384
GGTCCGG1450.049.0771941
CGGAAAG6100.048.9877475
GATCCGG3100.047.441291
TCGGGAA5550.046.1506233
CGGAGAG7850.044.7135165
AAGGGGC8850.043.945819
TGTGTTT527600.041.344781
Produced by FastQC (version 0.11.2)