##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2184150 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.780536593182703 33.0 33.0 33.0 27.0 33.0 2 31.606895588672938 33.0 33.0 33.0 27.0 33.0 3 31.556803790948425 33.0 33.0 33.0 27.0 33.0 4 35.20369296980519 37.0 37.0 37.0 33.0 37.0 5 35.19953391479523 37.0 37.0 37.0 33.0 37.0 6 35.353081519126434 37.0 37.0 37.0 33.0 37.0 7 35.2193979351235 37.0 37.0 37.0 33.0 37.0 8 35.17328709108806 37.0 37.0 37.0 33.0 37.0 9 34.96765103129364 37.0 37.0 37.0 33.0 37.0 10-11 34.924345626445074 37.0 37.0 37.0 33.0 37.0 12-13 34.92139962914635 37.0 37.0 37.0 33.0 37.0 14-15 36.59609459057299 40.0 37.0 40.0 33.0 40.0 16-17 36.46643843142641 40.0 37.0 40.0 33.0 40.0 18-19 36.54988302085479 40.0 37.0 40.0 33.0 40.0 20-21 36.48405214843303 40.0 37.0 40.0 33.0 40.0 22-23 36.70552457477737 40.0 37.0 40.0 33.0 40.0 24-25 36.37841906462468 40.0 37.0 40.0 33.0 40.0 26-27 36.18551999633725 40.0 37.0 40.0 30.0 40.0 28-29 36.05151409014948 40.0 37.0 40.0 27.0 40.0 30-31 36.12574868942151 40.0 37.0 40.0 33.0 40.0 32-33 36.1031534006364 40.0 37.0 40.0 33.0 40.0 34-35 35.96577867820434 40.0 37.0 40.0 27.0 40.0 36-37 35.810863722729664 37.0 37.0 40.0 27.0 40.0 38-39 35.775354256804704 37.0 37.0 40.0 27.0 40.0 40-41 35.59903532266557 37.0 37.0 40.0 27.0 40.0 42-43 35.485636746560445 37.0 37.0 40.0 27.0 40.0 44-45 35.35684774397363 37.0 37.0 40.0 27.0 40.0 46-47 35.2135585925875 37.0 37.0 40.0 27.0 40.0 48-49 35.06312890598173 37.0 37.0 40.0 27.0 40.0 50-51 33.8953833298995 37.0 33.0 38.5 24.5 38.5 52-53 34.36275324496944 37.0 33.0 38.5 27.0 40.0 54-55 35.11108646384177 37.0 37.0 40.0 27.0 40.0 56-57 35.13482292882815 37.0 37.0 40.0 27.0 40.0 58-59 34.900080809468214 37.0 35.0 40.0 27.0 40.0 60-61 34.68921754458256 37.0 33.0 40.0 27.0 40.0 62-63 34.44528992972094 37.0 33.0 40.0 27.0 40.0 64-65 34.1731497378843 37.0 33.0 37.0 27.0 40.0 66-67 33.87669322161939 37.0 33.0 37.0 27.0 40.0 68-69 33.70517386626376 37.0 33.0 37.0 27.0 40.0 70-71 33.40891422292425 37.0 33.0 37.0 24.5 37.0 72-73 33.2380072339354 37.0 33.0 37.0 22.0 37.0 74-75 33.06633152484948 37.0 33.0 37.0 24.5 37.0 76-77 32.79490053338827 37.0 33.0 37.0 22.0 37.0 78-79 32.60512716617448 37.0 33.0 37.0 22.0 37.0 80-81 32.42236522216881 37.0 33.0 37.0 22.0 37.0 82-83 32.07801501728361 35.0 33.0 37.0 22.0 37.0 84-85 31.93349334981572 33.0 33.0 37.0 22.0 37.0 86-87 31.758988164732276 33.0 33.0 37.0 22.0 37.0 88-89 31.713530206258728 33.0 33.0 37.0 15.0 37.0 90-91 31.65196277728178 33.0 33.0 37.0 15.0 37.0 92-93 31.4546642858778 33.0 33.0 37.0 15.0 37.0 94-95 31.23639379163519 33.0 33.0 37.0 15.0 37.0 96-97 31.0658066066891 33.0 33.0 37.0 15.0 37.0 98-99 30.94654327770529 33.0 33.0 37.0 15.0 37.0 100-101 29.53366710161848 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1286577546644878 2306 2 0.19913387769966562 2306 3 0.14484980800740388 2306 4 0.13393054566661533 2306 5 0.21720796118751906 2306 6 0.16263555661985407 2306 7 0.3983412890347253 2306 8 0.2060164931717452 2306 9 0.3196654722140906 2306 10-11 0.307238604900391 2306 12-13 0.2373035173794591 2306 14-15 0.49133311283190295 2306 16-17 0.5148414978225944 2306 18-19 0.4673501383578298 2306 20-21 0.45105411741688073 2306 22-23 0.347365373517853 2306 24-25 0.49792354098397595 2306 26-27 0.4019863114089901 2306 28-29 0.4165295798017823 2306 30-31 0.558426031293493 2306 32-33 0.4126668515927463 2306 34-35 0.3931452479423925 2306 36-37 0.49572389681434714 2306 38-39 0.37391090424090123 2306 40-41 0.47400572608847114 2306 42-43 0.42102237055060243 2306 44-45 0.40449415476316375 2306 46-47 0.4112923695286028 2306 48-49 0.5677839619653113 2306 50-51 0.37323818525499775 2306 52-53 0.4967924361256877 2306 54-55 0.5586557291001313 2306 56-57 0.5296747272050553 2306 58-59 0.6288279223541693 2306 60-61 0.5273941731312348 2306 62-63 0.646802582176548 2306 64-65 0.6119589078752128 2306 66-67 0.6079131419750112 2306 68-69 0.325426868975093 2306 70-71 0.3961295826891913 2306 72-73 0.329011408183014 2306 74-75 0.38897775866043816 2306 76-77 0.42967698100165563 2306 78-79 0.2719229495472817 2306 80-81 0.219473303600779 2306 82-83 0.3077858372028075 2306 84-85 0.30432971319537216 2306 86-87 0.3552113721946881 2306 88-89 0.3733134513205094 2306 90-91 0.215368251281987 2306 92-93 -0.13640082801011033 2306 94-95 -0.08531901026470479 2306 96-97 0.140751852550693 2306 98-99 0.2638297746206675 2306 100-101 -0.005320136811914011 2307 1 0.2344970266638633 2307 2 0.1759259142227947 2307 3 0.15749160676088536 2307 4 0.24076431564689216 2307 5 0.27797235648725405 2307 6 0.26576830888716074 2307 7 0.26435802523485563 2307 8 0.20015153081079973 2307 9 0.32987839446595757 2307 10-11 0.270672824194385 2307 12-13 0.140092426903891 2307 14-15 0.45578440952614585 2307 16-17 0.4211972865835989 2307 18-19 0.48147164172567614 2307 20-21 0.31834199129860963 2307 22-23 0.32596493437322493 2307 24-25 0.4677045841732195 2307 26-27 0.312638043768942 2307 28-29 0.19456084176051291 2307 30-31 0.292254483904145 2307 32-33 0.513240268491117 2307 34-35 0.4355718096353911 2307 36-37 0.4187722321591991 2307 38-39 0.4002938685874966 2307 40-41 0.5104129490941887 2307 42-43 0.4759134407414862 2307 44-45 0.47049647358591073 2307 46-47 0.6074551728287432 2307 48-49 0.46610164469469595 2307 50-51 0.13613287350513303 2307 52-53 0.24482200546423627 2307 54-55 0.2574477803416997 2307 56-57 0.42389215463648355 2307 58-59 0.48313413540755334 2307 60-61 0.5849864108955316 2307 62-63 0.7113638322802487 2307 64-65 0.5767542480182684 2307 66-67 0.48269453974476306 2307 68-69 0.2719380643529732 2307 70-71 0.4023576555641881 2307 72-73 0.319737120756038 2307 74-75 0.26477255422135215 2307 76-77 0.20480402786783003 2307 78-79 0.10578910142548636 2307 80-81 0.1905664619363634 2307 82-83 0.08950038784894332 2307 84-85 0.13188165589515677 2307 86-87 0.237209486896802 2307 88-89 0.18828430881660907 2307 90-91 0.24200778473431583 2307 92-93 -0.00904069329627788 2307 94-95 -0.10140542488037951 2307 96-97 0.1380185720253273 2307 98-99 -0.0212852365787235 2307 100-101 -0.014220107642646695 2201 1 0.17631430654089897 2201 2 0.10298455574284304 2201 3 0.048782957865590504 2201 4 0.20310106977993314 2201 5 0.20033902500164658 2201 6 0.19732694774522486 2201 7 0.2323882828549273 2201 8 0.21086458731710422 2201 9 0.2947606269456742 2201 10-11 0.27360209728932716 2201 12-13 0.14595645363893084 2201 14-15 0.3128118620466225 2201 16-17 0.39612003438487875 2201 18-19 0.3273529180851398 2201 20-21 0.3499587865701841 2201 22-23 0.19303744323840277 2201 24-25 0.0881477066057812 2201 26-27 0.14686188739739947 2201 28-29 0.16003763509666413 2201 30-31 0.35433293211440287 2201 32-33 0.46847267022572936 2201 34-35 0.47126675685461095 2201 36-37 0.5486954450238883 2201 38-39 0.38696001538530567 2201 40-41 0.28515299117851356 2201 42-43 0.4280781464690975 2201 44-45 0.5389378347278324 2201 46-47 0.5009554968072507 2201 48-49 0.6317761761002529 2201 50-51 0.333722028665008 2201 52-53 0.3853245495333013 2201 54-55 0.4391493048414645 2201 56-57 0.6423570194878039 2201 58-59 0.6516966009388412 2201 60-61 0.6971615828985236 2201 62-63 0.5936205829808969 2201 64-65 0.44300718050140375 2201 66-67 0.43927421606689876 2201 68-69 0.41285947865932116 2201 70-71 0.5570457295534297 2201 72-73 0.45225748567892055 2201 74-75 0.23835695289437098 2201 76-77 0.31445235537769634 2201 78-79 0.3865434937692598 2201 80-81 0.29380850191427754 2201 82-83 0.4533591050318293 2201 84-85 0.28915999211439214 2201 86-87 0.26152979751530836 2201 88-89 0.33536491636165167 2201 90-91 0.3035682431333768 2201 92-93 0.16481269053323544 2201 94-95 0.15379224585660722 2201 96-97 0.31079526361125076 2201 98-99 0.12732068497807703 2201 100-101 0.06643034224747524 1101 1 0.08435162042396271 1101 2 0.06754849119344897 1101 3 0.1297028586906599 1101 4 0.12595050417563414 1101 5 0.13772632455989253 1101 6 0.07388755832332095 1101 7 0.24405010997845977 1101 8 0.18842111495875002 1101 9 0.1856014600407434 1101 10-11 0.24740619386567886 1101 12-13 0.20363707526884411 1101 14-15 0.30521796164881465 1101 16-17 0.4164303771924054 1101 18-19 0.41510209772356177 1101 20-21 0.32735195595069655 1101 22-23 0.15494859208269673 1101 24-25 0.1343326281573809 1101 26-27 0.2605153737774657 1101 28-29 0.3692457949073926 1101 30-31 0.3226739262361349 1101 32-33 0.2794234444506145 1101 34-35 0.35187591266982565 1101 36-37 0.4153435171195312 1101 38-39 0.19247391774165834 1101 40-41 0.4317711837220912 1101 42-43 0.38528994009033823 1101 44-45 0.3097760680803816 1101 46-47 0.3683508491554335 1101 48-49 0.3227075921526179 1101 50-51 0.46001996906853293 1101 52-53 0.5426583736700437 1101 54-55 0.6081150823786956 1101 56-57 0.5437976324208265 1101 58-59 0.5808941286186666 1101 60-61 0.5362679767787526 1101 62-63 0.4859890562646072 1101 64-65 0.5369935563349912 1101 66-67 0.6184364053182421 1101 68-69 0.614993978029645 1101 70-71 0.5617300750522176 1101 72-73 0.4237393010336703 1101 74-75 0.44529660418496064 1101 76-77 0.5241331029144263 1101 78-79 0.468052999879383 1101 80-81 0.44859970790627557 1101 82-83 0.29990697045959536 1101 84-85 0.19490081818549498 1101 86-87 0.351143680373589 1101 88-89 0.34436346464444156 1101 90-91 0.4323838826248618 1101 92-93 0.5164192702134471 1101 94-95 0.5238577261430137 1101 96-97 0.3517298419064865 1101 98-99 0.32365720474471615 1101 100-101 0.13926262475438378 2304 1 0.1283955372765604 2304 2 0.07843132933512464 2304 3 0.10057391646828506 2304 4 0.2098643309202899 2304 5 0.22451605690658027 2304 6 0.07243520311196505 2304 7 0.2386776071141341 2304 8 0.22333498046877054 2304 9 0.25387561517112545 2304 10-11 0.25385430103899864 2304 12-13 0.12546294555131965 2304 14-15 0.25698846206662296 2304 16-17 0.3404742186679002 2304 18-19 0.30476079021083535 2304 20-21 0.3850991605370666 2304 22-23 0.1834115237781262 2304 24-25 0.09946479651967621 2304 26-27 0.08719797911157912 2304 28-29 0.010408260556964422 2304 30-31 0.21647270598855073 2304 32-33 0.24214664143036657 2304 34-35 0.2515745657887152 2304 36-37 0.32545497831539905 2304 38-39 0.1697625280463484 2304 40-41 0.1918853100368878 2304 42-43 0.1428862020322299 2304 44-45 0.2998439236233921 2304 46-47 0.2538927405176423 2304 48-49 0.25241413833078497 2304 50-51 0.1563419165113089 2304 52-53 0.25321919006865556 2304 54-55 0.3750598546537063 2304 56-57 0.3567411458982477 2304 58-59 0.39297309366953215 2304 60-61 0.40689314368518836 2304 62-63 0.3800340230125414 2304 64-65 0.3439654535981518 2304 66-67 0.36333108735609443 2304 68-69 0.28370031315193245 2304 70-71 0.2730330866904396 2304 72-73 0.18986380892888377 2304 74-75 0.2410701562985409 2304 76-77 0.23321181706722882 2304 78-79 0.25036406717605786 2304 80-81 0.16770236505676195 2304 82-83 0.2827756398599419 2304 84-85 0.29373783028029266 2304 86-87 0.222773381751054 2304 88-89 0.18001917542896706 2304 90-91 0.04047289544327981 2304 92-93 0.17816049201164574 2304 94-95 0.2282080078380062 2304 96-97 0.3964708623401414 2304 98-99 0.32842022990774566 2304 100-101 0.05554343245232474 2202 1 0.06528877997947902 2202 2 0.0625386863923012 2202 3 0.03234720715201078 2202 4 0.07004775754963788 2202 5 0.15735592096095274 2202 6 0.13071439143663355 2202 7 0.2711421213084506 2202 8 0.08035067419127984 2202 9 0.2640470465985558 2202 10-11 0.14460625025930796 2202 12-13 0.17983819545136015 2202 14-15 0.2054950594276832 2202 16-17 0.13140634581704802 2202 18-19 0.09551248953110303 2202 20-21 0.23694592183255736 2202 22-23 0.11991392281850466 2202 24-25 -0.03234348016965072 2202 26-27 0.1141999283318853 2202 28-29 0.07639086751418489 2202 30-31 0.26808156244597114 2202 32-33 0.24806102567666244 2202 34-35 0.3429175286035857 2202 36-37 0.45783752529660404 2202 38-39 0.2051578699096126 2202 40-41 0.23432158552668625 2202 42-43 0.22040394493506454 2202 44-45 0.2835991715242301 2202 46-47 0.26261100321256947 2202 48-49 0.1994693162596093 2202 50-51 0.21619622478799982 2202 52-53 0.1729238082213982 2202 54-55 0.13578756357014043 2202 56-57 0.3221377372617056 2202 58-59 0.30310604049081036 2202 60-61 0.42181967307343626 2202 62-63 0.32234384908211666 2202 64-65 0.3875435223706418 2202 66-67 0.20653555556881287 2202 68-69 0.2964302211887073 2202 70-71 0.36094492195633876 2202 72-73 0.2244344806601859 2202 74-75 0.3087355901250035 2202 76-77 0.13211600539190016 2202 78-79 0.2454742730936914 2202 80-81 0.22375344462493985 2202 82-83 0.35121376461004417 2202 84-85 0.2928469366377442 2202 86-87 0.4144429639181837 2202 88-89 0.2707536316464818 2202 90-91 0.21212498460761253 2202 92-93 0.4133249262379515 2202 94-95 0.2973578479019814 2202 96-97 0.3853900446672114 2202 98-99 0.4277509976006719 2202 100-101 0.15596526371981767 1102 1 -0.05242823891532922 1102 2 -0.15312082263672622 1102 3 -0.23215045785245536 1102 4 -0.09711869992857203 1102 5 -0.14746717344522864 1102 6 -0.18859992270789405 1102 7 -0.11289623367871116 1102 8 -0.20828847005829942 1102 9 -0.05744327984852049 1102 10-11 0.030722242434521263 1102 12-13 -0.029730876317245247 1102 14-15 -0.2336263490325834 1102 16-17 -0.10357256572450524 1102 18-19 -0.07377231207908608 1102 20-21 -0.09757187049418548 1102 22-23 -0.07628574474144756 1102 24-25 0.005088024743457709 1102 26-27 -0.03404198398988001 1102 28-29 -0.07177698078460537 1102 30-31 0.18201735854940182 1102 32-33 0.18876808267769718 1102 34-35 0.23851258497140293 1102 36-37 0.17978433488987378 1102 38-39 0.18244497824661465 1102 40-41 0.23038759862075864 1102 42-43 0.1956869147327751 1102 44-45 0.12212942469881227 1102 46-47 0.08777648824711548 1102 48-49 0.10514770878761937 1102 50-51 0.16717647061271634 1102 52-53 0.22889811761677947 1102 54-55 0.3703306846619938 1102 56-57 0.3926040579193426 1102 58-59 0.491571727929319 1102 60-61 0.4062255213043784 1102 62-63 0.48611817430797544 1102 64-65 0.43127815832618666 1102 66-67 0.3358655871166931 1102 68-69 0.28096949773586033 1102 70-71 0.29186569041296906 1102 72-73 0.309329264517622 1102 74-75 0.19372264305534515 1102 76-77 0.15417860362567382 1102 78-79 0.17368237603702852 1102 80-81 0.1910173726188802 1102 82-83 0.11781496094189237 1102 84-85 0.07958269203424351 1102 86-87 -0.06429557311948031 1102 88-89 -0.030828963873492654 1102 90-91 0.006763427793160304 1102 92-93 0.21374635618243332 1102 94-95 0.1946779740935085 1102 96-97 0.3699425509711354 1102 98-99 0.13695653374504602 1102 100-101 0.022134287996987467 2305 1 -0.04216610353796568 2305 2 -0.013740434246440003 2305 3 -0.08059384064411645 2305 4 0.056988586599182156 2305 5 0.07747404411528436 2305 6 0.04486729529751443 2305 7 0.20619626108751987 2305 8 -0.0016158575816618281 2305 9 0.09714777939960584 2305 10-11 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-0.5182093222214377 1213 86-87 -0.673899574817824 1213 88-89 -0.6488703317274194 1213 90-91 -0.7148062762881615 1213 92-93 -0.5195282598089399 1213 94-95 -0.45376146389233796 1213 96-97 -0.4746752892526622 1213 98-99 -0.4526812490063641 1213 100-101 -0.3537136062151731 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2306.0 3 218.0 4 211.0 5 180.0 6 421.0 7 2714.0 8 13175.0 9 20671.0 10 20051.0 11 14535.0 12 9468.0 13 6829.0 14 5355.0 15 5188.0 16 5237.0 17 5439.0 18 5942.0 19 6452.0 20 7236.0 21 7946.0 22 8710.0 23 9413.0 24 11043.0 25 12949.0 26 15519.0 27 18454.0 28 22706.0 29 27991.0 30 35118.0 31 46901.0 32 64458.0 33 93642.0 34 143872.0 35 253573.0 36 476403.0 37 657316.0 38 146382.0 39 126.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.99888945894624 20.84563571132908 27.04585066068015 12.10962416904454 2 36.6736002805268 19.10593099371293 33.211949193072385 11.00851953268789 3 43.395498262438146 15.371628985459363 27.147815049814085 14.085057702288406 4 35.86926247580137 13.779280972065639 35.03355803243064 15.317898519702352 5 34.742812564532045 12.829222403230617 36.842622123304956 15.58534290893238 6 27.26481591894568 13.385860382881983 43.98126027281569 15.368063425356649 7 37.780261958715485 13.865997406294827 31.354508834291977 16.99923180069771 8 33.8202567865071 26.65047871017593 23.14418935871282 16.38507514460415 9 34.80542957360366 27.787359288291825 20.288500581543396 17.11871055656112 10-11 31.13042832029888 26.56585456707391 26.145874238025858 16.157842874601354 12-13 31.69108668828532 22.77662781152219 26.2641840855581 19.26810141463439 14-15 29.35346667923911 23.424435369954473 26.304695004679107 20.917402946127307 16-17 27.80682643094507 26.157852038320666 27.58716813793825 18.448153392796016 18-19 27.092686908432135 26.442388094899993 27.010513473850978 19.45441152281689 20-21 25.894007574918216 27.814071106786344 27.322144145495685 18.969777172799756 22-23 27.51911533776545 25.315004072557567 27.261416002582955 19.90446458709403 24-25 27.666784462377613 25.19397180449225 27.674951938429654 19.46429179470048 26-27 23.80767286928555 26.187916379508387 29.363909947162874 20.64050080404319 28-29 23.365306359993063 27.268134981411045 27.192753230465726 22.173805428130162 30-31 22.920777720954362 27.39519696432824 27.80076612867113 21.88325918604627 32-33 22.53615681474022 24.928897169189472 30.06160369020188 22.473342325868426 34-35 22.378088161737455 26.73666197422242 28.44347251289924 22.44177735114088 36-37 21.03417953648658 29.75317472663241 28.367432142638037 20.845213594242974 38-39 20.450272933765714 28.089959924987447 27.801264515761414 23.65850262548543 40-41 22.58042502426399 27.67825302932267 26.36889356195406 23.372428384459283 42-43 25.018529672765062 27.156201003269132 26.73661656451591 21.088652759449904 44-45 21.777353018021984 29.194545355525527 26.40947611542139 22.6186255110311 46-47 22.35050573977701 29.359705793190592 27.114887244242812 21.174901222789583 48-49 22.20790115398494 27.185802836582774 28.598279479936846 22.00801652949545 50-51 20.70537551558572 27.24110838747405 27.19511253238789 24.858403564552344 52-53 22.553388789647215 28.37602589851389 26.532161313554674 22.53842399828422 54-55 23.286181031198282 27.97557468160821 27.485101272739893 21.253143014453617 56-57 24.815963673294068 27.458649713100748 25.06318402437592 22.662202589229263 58-59 22.94835009646509 26.183946691977024 25.99490039218029 24.87280281937759 60-61 20.75172628779266 26.05809570913047 28.514444903629045 24.67573309944782 62-63 22.337063150314503 26.09370866645112 28.422716367350535 23.14651181588384 64-65 22.985117343360915 28.293608655475445 27.157557979148706 21.56371602201493 66-67 22.207938751169273 27.777106485267606 26.843662763879216 23.171291999683906 68-69 21.24628863369808 24.783542872683658 30.646020069286493 23.32414842433177 70-71 21.084754558752277 28.98525997689911 28.34745947584395 21.582525988504663 72-73 23.4861944645605 29.864151161738782 26.51816805433035 20.131486319370364 74-75 24.78051114902095 28.121233303105992 26.87738086244141 20.220874685431653 76-77 23.32856656327055 27.33614409715484 26.619268422127153 22.71602091744746 78-79 23.657967446250943 28.821804167633204 26.347636557396253 21.1725918287196 80-81 22.679169199008285 29.702724914428675 26.245142612751792 21.372963273811248 82-83 22.43711457962171 29.34328261515315 26.914245451916052 21.305357353309088 84-85 22.866131939586747 28.50588331539369 26.829958810817466 21.798025934202098 86-87 20.586516517751782 29.201411621634477 27.946031692776273 22.266040167837463 88-89 19.987908621162305 29.49439331816894 28.472095041638507 22.04560301903025 90-91 21.524838083414867 28.597778136660747 27.083286909549777 22.79409687037461 92-93 21.95008065103287 28.769255821677042 25.9842859679146 23.29637755937549 94-95 22.621242427909426 30.040806223138468 25.7078746527246 21.63007669622751 96-97 21.28346947301118 31.33730449179407 24.650012665847388 22.729213369347363 98-99 21.585902780091043 29.185407939277475 26.44738066330264 22.781308617328847 100-101 23.166473471797953 29.869619312287536 26.130615048980353 20.833292166934154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1744.0 1 1101.5 2 368.0 3 254.5 4 218.0 5 202.0 6 240.5 7 312.5 8 406.5 9 554.5 10 688.5 11 765.5 12 795.5 13 754.5 14 661.0 15 590.0 16 546.5 17 510.5 18 510.5 19 557.0 20 621.5 21 680.0 22 741.5 23 839.5 24 1025.5 25 1229.5 26 1493.0 27 1924.0 28 2395.0 29 2979.5 30 3775.0 31 4908.0 32 6548.0 33 9205.5 34 13888.0 35 22909.5 36 34551.0 37 47289.0 38 63077.0 39 77011.0 40 86747.5 41 94002.0 42 128699.0 43 165369.0 44 155749.5 45 134123.0 46 131877.0 47 128562.0 48 113452.0 49 102888.5 50 93360.5 51 87963.0 52 86522.5 53 87035.5 54 74558.0 55 47486.0 56 31218.0 57 25272.5 58 21820.5 59 17996.5 60 13940.0 61 10942.0 62 8380.5 63 6428.0 64 5163.5 65 3958.5 66 2983.0 67 2392.0 68 1879.5 69 1346.0 70 984.0 71 806.0 72 603.5 73 372.0 74 276.0 75 213.5 76 168.0 77 116.0 78 84.5 79 78.0 80 68.0 81 50.0 82 41.5 83 38.0 84 26.5 85 18.0 86 15.5 87 15.5 88 16.0 89 13.5 90 8.0 91 6.0 92 5.5 93 3.0 94 2.5 95 2.0 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2303871071126067 2 0.24686949156422408 3 0.22649543300597486 4 0.21935306641027402 5 0.22892200627246295 6 0.25012018405329306 7 0.23020396950758876 8 0.2175674747613488 9 0.2108371677769384 10-11 0.1962319437767553 12-13 0.2224664056955795 14-15 0.21887233019710187 16-17 0.22232905249181603 18-19 0.2112263351876016 20-21 0.20403818419064626 22-23 0.1694938534441316 24-25 0.49886683606895127 26-27 0.35025066959686835 28-29 0.48288808003113337 30-31 0.3131424123801021 32-33 0.3617196621111187 34-35 0.36428358858137033 36-37 0.3706934047569993 38-39 0.3654053064121054 40-41 0.3702126685438271 42-43 0.4239635556166015 44-45 0.37907195018657147 46-47 0.39711100428084156 48-49 0.39580614884508847 50-51 0.40995352883272673 52-53 0.4140970171462583 54-55 0.37969003960350706 56-57 0.3919373669390838 58-59 0.3888240276537783 60-61 0.34734336011720807 62-63 0.30867843325778904 64-65 0.3367213790261658 66-67 0.34732046791658083 68-69 0.3383925096719548 70-71 0.30881578646155255 72-73 0.2980106677654923 74-75 0.30957122908225165 76-77 0.317034086486734 78-79 0.32667170295080467 80-81 0.33472975757159534 82-83 0.35306641027401964 84-85 0.38165876885745026 86-87 0.3825057802806584 88-89 0.3763248861113019 90-91 0.34995307098871414 92-93 0.3286633244053751 94-95 0.3050843577593114 96-97 0.3221161550259827 98-99 0.35219650665018426 100-101 0.36730535906416684 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2184150.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.706463502163174 #Duplication Level Percentage of deduplicated Percentage of total 1 87.76945813961729 40.99400993202673 2 5.691424113076833 5.316525852255091 3 1.8370516320046815 2.5740655500544785 4 1.0739667615169153 2.006447573973048 5 0.5877068024112861 1.3724853158397883 6 0.39190271289590173 1.09826338537627 7 0.2745516927293285 0.897633703114365 8 0.30094842577060427 1.1244989331430553 9 0.1812832954816465 0.7620391461568846 >10 1.5489606952160997 14.247067663567172 >50 0.18813008308805448 6.101510700754894 >100 0.14047512719191085 12.621713204050053 >500 0.008770962606119955 2.7247704468037357 >1k 0.004576655093227574 3.3779288142555526 >5k 2.9733798757091795E-4 0.762151809829911 >10k+ 4.955633125876602E-4 4.018887968799035 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31458 1.440285694663828 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 21645 0.9910033651534922 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12018 0.550236934276492 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11437 0.5236361971476318 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10258 0.46965638806858506 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5851 0.2678845317400362 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5467 0.250303321658311 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5146 0.2356065288556189 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 3097 0.14179429068516355 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2836 0.129844561957741 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2752 0.12599867225236364 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2688 0.12306847057207611 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 2576 0.11794061763157292 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2444 0.1118970766659799 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2289 0.10480049447153354 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2267 0.10379323764393471 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2235 0.10232813680379094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003067554884051004 0.0 0.0 2 0.0034796144953414374 0.0 0.0 3 0.0038916741066318703 0.0 0.0 4 0.003983242909140856 0.0 0.0 5 0.004349518119176796 0.0 0.0 6 0.004853146532976215 0.0 0.0 7 0.005265206144266649 0.0 0.0 8 0.005631481354302589 0.0 0.0 9 0.006592953780646934 0.0 0.0 10-11 0.009774969667834168 0.0 0.0 12-13 0.013071446558157635 0.0 0.0 14-15 0.016894444062907767 0.0 4.578440125449259E-5 16-17 0.02177048279651123 0.0 4.578440125449259E-5 18-19 0.025936863310670053 0.0 4.578440125449259E-5 20-21 0.029210448000366276 0.0 4.578440125449259E-5 22-23 0.032621385893825974 0.0 4.578440125449259E-5 24-25 0.03639859899732161 0.0 4.578440125449259E-5 26-27 0.04051919511022595 0.0 4.578440125449259E-5 28-29 0.0457386168532381 0.0 4.578440125449259E-5 30-31 0.10026783874733879 0.0 4.578440125449259E-5 32-33 0.1083945699700112 0.0 4.578440125449259E-5 34-35 0.11505620035253988 0.0 4.578440125449259E-5 36-37 0.12295400956893987 0.0 4.578440125449259E-5 38-39 0.1306915733809491 0.0 4.578440125449259E-5 40-41 0.14000869903623836 0.0 4.578440125449259E-5 42-43 0.1513632305473525 0.0 4.578440125449259E-5 44-45 0.1635647734816748 0.0 4.578440125449259E-5 46-47 0.17718563285488634 0.0 4.578440125449259E-5 48-49 0.19185953345695123 0.0 4.578440125449259E-5 50-51 0.21010461735686653 0.0 4.578440125449259E-5 52-53 0.23057024471762472 0.0 4.578440125449259E-5 54-55 0.25561431220383213 0.0 4.578440125449259E-5 56-57 0.28265000114461003 0.0 4.578440125449259E-5 58-59 0.3145159444177369 0.0 4.578440125449259E-5 60-61 0.3495410113774237 0.0 4.578440125449259E-5 62-63 0.38896138085754184 0.0 4.578440125449259E-5 64-65 0.43202161023739216 0.0 4.578440125449259E-5 66-67 0.4785385619119566 0.0 4.578440125449259E-5 68-69 0.5304809651351785 0.0 4.578440125449259E-5 70-71 0.5901380399697823 0.0 4.578440125449259E-5 72-73 0.6546482613373623 0.0 4.578440125449259E-5 74-75 0.7248586406611268 0.0 4.578440125449259E-5 76-77 0.8037222718219903 0.0 4.578440125449259E-5 78-79 0.8964585765629649 0.0 4.578440125449259E-5 80-81 0.9981686239498203 0.0 4.578440125449259E-5 82-83 1.1128585490923242 0.0 4.578440125449259E-5 84-85 1.2373234439026624 0.0 4.578440125449259E-5 86-87 1.376622484719456 0.0 4.578440125449259E-5 88-89 1.5303893963326693 0.0 4.578440125449259E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 905 0.0 66.558266 9 AAGCGGC 340 0.0 65.56405 9 AGAGCGG 985 0.0 63.078583 8 GTTCGGA 255 0.0 57.663136 2 CGGGAGA 1325 0.0 57.63506 4 GGTTCGG 330 0.0 57.504597 1 TCGGAGA 495 0.0 57.494038 4 ATTCGGA 240 0.0 57.31437 2 TCGGGAG 1325 0.0 54.413223 3 GGATCGG 715 0.0 53.74468 1 ATCGGGA 1665 0.0 52.417988 2 TTGTGTT 29105 0.0 52.143852 1 TCGGAAA 455 0.0 51.081238 4 GGTCCGG 145 0.0 49.077194 1 CGGAAAG 610 0.0 48.987747 5 GATCCGG 310 0.0 47.44129 1 TCGGGAA 555 0.0 46.150623 3 CGGAGAG 785 0.0 44.713516 5 AAGGGGC 885 0.0 43.94581 9 TGTGTTT 52760 0.0 41.34478 1 >>END_MODULE