##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_53115004-1.3410000003588d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4584308 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65587368911513 33.0 33.0 33.0 33.0 33.0 2 32.6746429341135 33.0 33.0 33.0 33.0 33.0 3 32.71861576490934 33.0 33.0 33.0 33.0 33.0 4 36.640618387769756 37.0 37.0 37.0 37.0 37.0 5 36.64894352648208 37.0 37.0 37.0 37.0 37.0 6 36.530201722920886 37.0 37.0 37.0 37.0 37.0 7 36.387842832549644 37.0 37.0 37.0 37.0 37.0 8 36.33991258877021 37.0 37.0 37.0 37.0 37.0 9 36.38788667777121 37.0 37.0 37.0 37.0 37.0 10-11 36.31038817636163 37.0 37.0 37.0 35.0 37.0 12-13 36.334527261257314 37.0 37.0 37.0 37.0 37.0 14-15 38.18783827351915 40.0 37.0 40.0 37.0 40.0 16-17 38.20753230367593 40.0 37.0 40.0 37.0 40.0 18-19 38.16856786236876 40.0 37.0 40.0 37.0 40.0 20-21 38.1921621540263 40.0 37.0 40.0 37.0 40.0 22-23 37.911385753313255 40.0 37.0 40.0 33.0 40.0 24-25 38.06455390868153 40.0 37.0 40.0 37.0 40.0 26-27 38.039745148013616 40.0 37.0 40.0 37.0 40.0 28-29 37.82022477547321 40.0 37.0 40.0 33.0 40.0 30-31 37.77705773695834 40.0 37.0 40.0 33.0 40.0 32-33 37.59449997687764 40.0 37.0 40.0 33.0 40.0 34-35 37.54933220019248 40.0 37.0 40.0 33.0 40.0 36-37 37.58981922680588 40.0 37.0 40.0 33.0 40.0 38-39 37.381244453906675 40.0 37.0 40.0 33.0 40.0 40-41 37.09939111857231 40.0 37.0 40.0 33.0 40.0 42-43 36.92229699662414 37.0 37.0 40.0 33.0 40.0 44-45 36.81101084394853 37.0 37.0 40.0 33.0 40.0 46-47 37.03827600588791 37.0 37.0 40.0 33.0 40.0 48-49 36.72965614439518 37.0 37.0 40.0 33.0 40.0 50-51 36.70632426966077 37.0 37.0 40.0 33.0 40.0 52-53 36.609930113770716 37.0 37.0 40.0 33.0 40.0 54-55 36.29335692540728 37.0 37.0 40.0 33.0 40.0 56-57 36.209202348533296 37.0 37.0 40.0 33.0 40.0 58-59 36.09956148234368 37.0 37.0 40.0 33.0 40.0 60-61 35.8100170189263 37.0 37.0 40.0 30.0 40.0 62-63 35.583632360652906 37.0 35.0 40.0 27.0 40.0 64-65 35.211672077879584 37.0 33.0 38.5 27.0 40.0 66-67 34.38851785263992 37.0 33.0 37.0 27.0 40.0 68-69 34.22011479158905 37.0 33.0 37.0 27.0 40.0 70-71 33.603135413240125 37.0 33.0 37.0 27.0 38.5 72-73 32.265474636520935 37.0 33.0 37.0 18.5 37.0 74-75 31.346072406129778 35.0 27.0 37.0 15.0 37.0 76-77 28.814568196552237 33.0 27.0 33.0 15.0 37.0 78-79 30.722625639464013 33.0 30.0 37.0 15.0 37.0 80-81 30.969471619271655 35.0 33.0 37.0 15.0 37.0 82-83 30.87251052939724 35.0 33.0 37.0 15.0 37.0 84-85 30.657316764056866 33.0 33.0 37.0 15.0 37.0 86-87 30.48144540026543 33.0 33.0 37.0 15.0 37.0 88-89 30.34826335839564 33.0 27.0 37.0 15.0 37.0 90-91 30.20683099826626 33.0 27.0 37.0 6.0 37.0 92-93 30.07582965629709 33.0 27.0 37.0 6.0 37.0 94-95 29.907721514348513 33.0 27.0 37.0 6.0 37.0 96-97 29.754388121391493 33.0 27.0 37.0 6.0 37.0 98-99 29.61789194792322 33.0 27.0 37.0 6.0 37.0 100-101 28.225571340320066 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16515994657360977 2306 2 0.13432404280550259 2306 3 0.09387272542196712 2306 4 0.20041662179117026 2306 5 0.16658801593928274 2306 6 0.172113574661104 2306 7 0.21094519519470367 2306 8 0.10286548406121909 2306 9 0.2340032085099537 2306 10-11 0.25676989830245134 2306 12-13 0.23347891404348786 2306 14-15 0.41993673824817535 2306 16-17 0.34632603692531205 2306 18-19 0.42478856118881936 2306 20-21 0.4339603294609944 2306 22-23 0.42871081580428694 2306 24-25 0.4439932305177834 2306 26-27 0.4695525077170899 2306 28-29 0.48945277062514236 2306 30-31 0.5464154097048279 2306 32-33 0.5123752174208036 2306 34-35 0.5027322957107785 2306 36-37 0.46589379626252736 2306 38-39 0.5354523473235488 2306 40-41 0.5838690713002777 2306 42-43 0.5886392513875833 2306 44-45 0.555210667120484 2306 46-47 0.5108198978927163 2306 48-49 0.44154373151600623 2306 50-51 0.4652101059671736 2306 52-53 0.5317122418247635 2306 54-55 0.5810497801308756 2306 56-57 0.47942813376584326 2306 58-59 0.37982867637008866 2306 60-61 0.39413065709331363 2306 62-63 0.439924137999796 2306 64-65 0.35311781686041144 2306 66-67 0.2560006647459332 2306 68-69 0.3711307214135289 2306 70-71 0.3378095333558946 2306 72-73 -0.146682833647084 2306 74-75 -0.07015757199726735 2306 76-77 -0.399106048660439 2306 78-79 0.04344559634357381 2306 80-81 0.16141088419843896 2306 82-83 0.16702797511993595 2306 84-85 0.21232443360044684 2306 86-87 0.0681810859398162 2306 88-89 0.08415853418675923 2306 90-91 0.14873401910157824 2306 92-93 0.18648816443210947 2306 94-95 0.11877824141346949 2306 96-97 0.14788242032360088 2306 98-99 0.16308699117234227 2306 100-101 0.24228875344567413 2307 1 0.11669747203762171 2307 2 0.13130080035480063 2307 3 0.13395293124712992 2307 4 0.16615351628538377 2307 5 0.1265469676841846 2307 6 0.1341448654985129 2307 7 0.15821037489319423 2307 8 0.17408198232716643 2307 9 0.20487947614939372 2307 10-11 0.1624674637674275 2307 12-13 0.1590830064497979 2307 14-15 0.344883840773349 2307 16-17 0.32273394851539194 2307 18-19 0.2714885280091508 2307 20-21 0.2902795039730748 2307 22-23 0.2863717742703855 2307 24-25 0.3673647621765568 2307 26-27 0.42849440529757743 2307 28-29 0.40392348539676703 2307 30-31 0.41606541980701905 2307 32-33 0.40415099236997065 2307 34-35 0.31508840901842206 2307 36-37 0.33017180481508746 2307 38-39 0.3658486312006417 2307 40-41 0.39279472659873704 2307 42-43 0.21726665575506843 2307 44-45 0.4734358745860945 2307 46-47 0.36547510737875655 2307 48-49 0.35687568112739854 2307 50-51 0.35293666378969846 2307 52-53 0.301117598209629 2307 54-55 0.20779368220821937 2307 56-57 0.3094736214235638 2307 58-59 0.3234851036797366 2307 60-61 0.34035549661678743 2307 62-63 0.38691787927622556 2307 64-65 0.3301576945590412 2307 66-67 0.20185130618863667 2307 68-69 0.17819453852126088 2307 70-71 0.17477177711709402 2307 72-73 -0.03853356406320074 2307 74-75 -0.09330826886299093 2307 76-77 -0.29752019024309106 2307 78-79 0.10984362817752213 2307 80-81 0.23968906791741773 2307 82-83 0.21347788957570657 2307 84-85 0.3049974266475495 2307 86-87 0.23526493133368476 2307 88-89 0.19869639608148404 2307 90-91 0.08662107662103935 2307 92-93 0.2589710277819215 2307 94-95 0.19439243600944422 2307 96-97 0.2384634954931606 2307 98-99 0.17992717916575174 2307 100-101 0.19108652336413456 2201 1 0.0991473085953416 2201 2 0.10407477948257338 2201 3 0.10984457390415514 2201 4 0.10691832342681096 2201 5 0.055318897363413555 2201 6 0.06955058009664583 2201 7 0.13691193244094535 2201 8 0.05151414922550401 2201 9 0.1423019176937217 2201 10-11 0.16291117268940525 2201 12-13 0.1276551604583105 2201 14-15 0.21635001244656138 2201 16-17 0.23084571387425257 2201 18-19 0.17789338604715255 2201 20-21 0.19171306174615665 2201 22-23 0.2686117163158883 2201 24-25 0.2505104710090649 2201 26-27 0.25896249387616166 2201 28-29 0.266233617326904 2201 30-31 0.3165406284382257 2201 32-33 0.32002699079270513 2201 34-35 0.24586453617370552 2201 36-37 0.28682398009679133 2201 38-39 0.3187017882957619 2201 40-41 0.27024553237828286 2201 42-43 0.2922816289896346 2201 44-45 0.20604548777546228 2201 46-47 0.25687276666663905 2201 48-49 0.25654165166636744 2201 50-51 0.29489715516030657 2201 52-53 0.2224696999298672 2201 54-55 0.16903713167697276 2201 56-57 0.23295645791335318 2201 58-59 0.28450685401150366 2201 60-61 0.11098335354307665 2201 62-63 0.12474016624210549 2201 64-65 0.1436865608014628 2201 66-67 0.25003053601349734 2201 68-69 0.018211118841591656 2201 70-71 -0.04548263946935549 2201 72-73 -0.3053182907840686 2201 74-75 -0.2578398862042768 2201 76-77 -0.11400176591612166 2201 78-79 0.10167462074350908 2201 80-81 0.14634234082211606 2201 82-83 0.28494453757587834 2201 84-85 0.24691846571961307 2201 86-87 0.05050029069959905 2201 88-89 -0.055947274071616704 2201 90-91 -0.016420664532123652 2201 92-93 -0.10200476735274933 2201 94-95 -0.16023402424334776 2201 96-97 -0.039824009663593785 2201 98-99 -0.12397263394840152 2201 100-101 -0.13861322911152385 1101 1 0.0752815063645258 1101 2 0.10127754719151483 1101 3 0.07193409300587206 1101 4 0.14400098204725253 1101 5 0.08082482476445563 1101 6 0.10154653191059992 1101 7 0.09880179921341181 1101 8 0.06307691299799956 1101 9 0.1308094476082502 1101 10-11 0.12027220891694412 1101 12-13 0.05414380273300878 1101 14-15 0.1312092032365868 1101 16-17 0.14084491782106312 1101 18-19 0.049089059753711695 1101 20-21 0.15196955884558605 1101 22-23 0.12399404915704082 1101 24-25 0.037435744802976956 1101 26-27 0.17343995342944396 1101 28-29 0.1954615224234928 1101 30-31 0.23052694508736948 1101 32-33 0.09768390501670154 1101 34-35 0.13901320220949032 1101 36-37 0.023114520154969398 1101 38-39 0.06350982353973933 1101 40-41 -0.015687231071048302 1101 42-43 0.02236619503383963 1101 44-45 -0.08352889946898046 1101 46-47 0.05409410286381444 1101 48-49 0.02666714506871415 1101 50-51 0.04459804182844351 1101 52-53 0.04651072515142829 1101 54-55 -0.024918852789767243 1101 56-57 0.011399702881739415 1101 58-59 0.02644581133885282 1101 60-61 0.07708029748395262 1101 62-63 -0.006041923145545525 1101 64-65 -0.07312646584203009 1101 66-67 -0.069954543466757 1101 68-69 -0.16105512826609925 1101 70-71 -0.2677882961392868 1101 72-73 -0.5484676232405263 1101 74-75 -0.48544545864958977 1101 76-77 -0.49699728483050976 1101 78-79 -0.3294766839529686 1101 80-81 -0.04049211717977741 1101 82-83 -0.11057663168748633 1101 84-85 -0.15149894038490785 1101 86-87 -0.06079880784320579 1101 88-89 -0.15427915010422666 1101 90-91 -0.1429611689413619 1101 92-93 -0.22082410363062976 1101 94-95 -0.2427952377205287 1101 96-97 -0.09843497719502992 1101 98-99 -0.17766149144958732 1101 100-101 -0.240516252671231 2304 1 0.0762400315909062 2304 2 0.08247132106316712 2304 3 0.07106564683316208 2304 4 0.11937414503899646 2304 5 0.10030729068259348 2304 6 0.044548485105877944 2304 7 0.021833614342732233 2304 8 0.09592665765946151 2304 9 0.07824815807079943 2304 10-11 0.08944515948707021 2304 12-13 0.04793315925401487 2304 14-15 0.14550977860239556 2304 16-17 0.14440894328739518 2304 18-19 0.08642436124159047 2304 20-21 0.10739793366662553 2304 22-23 0.10006880183384936 2304 24-25 0.14251862117109937 2304 26-27 0.14769476960632488 2304 28-29 0.1285968273883924 2304 30-31 0.1148386752157009 2304 32-33 0.16007885153327095 2304 34-35 0.2194002672131674 2304 36-37 0.07537690733883551 2304 38-39 0.11625531423001689 2304 40-41 0.10957723464251501 2304 42-43 0.08399142654873515 2304 44-45 0.21728880648274185 2304 46-47 0.08378877611094993 2304 48-49 0.1395450131546454 2304 50-51 0.041270574954126005 2304 52-53 0.046710168585782696 2304 54-55 0.12002246846941489 2304 56-57 0.0394892870666439 2304 58-59 -0.016346995112868967 2304 60-61 0.11286577834154343 2304 62-63 0.23957879147891958 2304 64-65 0.069284151518886 2304 66-67 0.044412418706109236 2304 68-69 0.05025929412622787 2304 70-71 -0.026206866979308074 2304 72-73 -0.1563886366864793 2304 74-75 -0.0814064157678338 2304 76-77 -0.24252387735580783 2304 78-79 0.16963089461452086 2304 80-81 0.31600216615603927 2304 82-83 0.3697627658097886 2304 84-85 0.3054282318322521 2304 86-87 0.2996416900497607 2304 88-89 0.28297925265830415 2304 90-91 0.0872756767068843 2304 92-93 0.05885449851065161 2304 94-95 0.1223347886441708 2304 96-97 0.19639954701735363 2304 98-99 0.2292441919807615 2304 100-101 0.28296033789319 2202 1 0.055771551175688217 2202 2 0.09362544691336439 2202 3 0.022761758395809295 2202 4 0.022475127486131896 2202 5 0.0536553108163389 2202 6 0.05026849480201889 2202 7 0.030038972886025306 2202 8 0.0963337529525603 2202 9 0.12251531524808712 2202 10-11 0.10705167443767039 2202 12-13 0.06457433790539824 2202 14-15 0.03364002770764074 2202 16-17 0.04050446452622225 2202 18-19 0.008713363252113027 2202 20-21 -0.0013883020921667821 2202 22-23 0.08860864156235237 2202 24-25 -0.07442199710042274 2202 26-27 0.07179645074590013 2202 28-29 -0.007455346400661256 2202 30-31 -0.02598385690276217 2202 32-33 -0.03243088025438823 2202 34-35 -0.032253601974495894 2202 36-37 -0.09026934752705529 2202 38-39 -0.05633580096868229 2202 40-41 0.033699591374912075 2202 42-43 -0.13059663008812805 2202 44-45 -0.08414226536850578 2202 46-47 -0.16667268173223704 2202 48-49 -0.28929423054641745 2202 50-51 -0.052844188845050155 2202 52-53 0.052675611827716295 2202 54-55 -0.10539371695500677 2202 56-57 -0.0807711897553034 2202 58-59 -0.16000261816116534 2202 60-61 -0.21975210818509083 2202 62-63 -0.3288827305298909 2202 64-65 -0.298052333059978 2202 66-67 0.026883437228583773 2202 68-69 -0.26542813368743623 2202 70-71 -0.26100032493656045 2202 72-73 -0.2561194975040948 2202 74-75 -0.17345504618804952 2202 76-77 -0.4061654909075898 2202 78-79 -0.029353506007169017 2202 80-81 0.08271321405212362 2202 82-83 0.11211368765604846 2202 84-85 0.15915429448643081 2202 86-87 0.14289912969841723 2202 88-89 0.06215964364089288 2202 90-91 0.08851407342160655 2202 92-93 0.1114601579194705 2202 94-95 0.11619984118869553 2202 96-97 0.0028629868656331325 2202 98-99 -0.05065610175525137 2202 100-101 -0.06207359859709172 1102 1 0.07635706531790021 1102 2 0.07234479214700684 1102 3 0.07011311675670129 1102 4 -0.03309410228558818 1102 5 0.03341057144108106 1102 6 0.007785240521450021 1102 7 0.0035592896955876085 1102 8 -0.021093017255772395 1102 9 0.0035027515943966137 1102 10-11 0.010922454341802279 1102 12-13 0.05486524958076444 1102 14-15 -0.05619614354508684 1102 16-17 -0.07507104956611244 1102 18-19 -0.11267851401410667 1102 20-21 -0.12596280121685055 1102 22-23 -0.07265993084947553 1102 24-25 0.04007988320820033 1102 26-27 -0.016377181042791733 1102 28-29 -0.09350427931303784 1102 30-31 0.013047135770513307 1102 32-33 -0.06798816482936587 1102 34-35 -0.08253620936904582 1102 36-37 -0.07990526840446677 1102 38-39 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0.11455076209713866 1213 68-69 0.06523407672287362 1213 70-71 -0.01468549013758036 1213 72-73 0.5959813910684488 1213 74-75 0.5479603844367098 1213 76-77 0.27207079591536143 1213 78-79 -0.2637337438782694 1213 80-81 -0.3237744274421459 1213 82-83 -0.24535063741915053 1213 84-85 -0.2742984814417504 1213 86-87 -0.2350402487600114 1213 88-89 -0.3566836335156758 1213 90-91 -0.3201449167742467 1213 92-93 -0.17727009602399946 1213 94-95 -0.05841700495278701 1213 96-97 -0.1725201669928822 1213 98-99 -0.20533416641210422 1213 100-101 0.12842203316191458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 9.0 8 86.0 9 241.0 10 186.0 11 89.0 12 76.0 13 149.0 14 323.0 15 773.0 16 1556.0 17 2710.0 18 4110.0 19 6049.0 20 8578.0 21 12187.0 22 16542.0 23 22493.0 24 30500.0 25 41741.0 26 58462.0 27 86695.0 28 148214.0 29 246775.0 30 215288.0 31 114490.0 32 122346.0 33 172275.0 34 265159.0 35 460498.0 36 899547.0 37 1331655.0 38 314431.0 39 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4606455942824 23.01615667919661 19.776934097859396 13.7462636286616 2 26.5353462289183 32.52486525774446 28.31319361613574 12.626594897201496 3 32.68173138947843 17.185101417141517 34.630538207499114 15.50262898588094 4 25.86787323446528 17.11987856951821 28.68672968847008 28.325518507546434 5 40.837373928627834 15.992053762530789 27.41951457013796 15.75105773870342 6 35.717168218191276 17.074136380016352 31.433402816739193 15.775292585053185 7 28.491781093242423 30.04004093965763 24.355257107506738 17.112920859593203 8 25.073882470375025 35.96784945514132 22.238601769340104 16.719666305143548 9 38.18534880291639 21.766949341100116 22.588403745996125 17.459298109987376 10-11 27.187363938025104 31.70673741816649 25.70155626541672 15.404342378391679 12-13 20.574318244532158 30.345592189001387 25.674949637949485 23.40513992851697 14-15 20.181104759976858 30.848570820285197 25.987291866078806 22.983032553659136 16-17 26.004944695021514 24.811078315653816 26.55123228003709 22.632744709287575 18-19 18.761708419242336 24.95196657816185 33.16933548094936 23.116989521646452 20-21 25.216194025357808 25.153861826037865 32.81824650525226 16.811697643352062 22-23 18.54915488197163 37.951155252584336 26.460994430884277 17.038695434559756 24-25 18.783842356107478 24.510902084000918 33.02899434963671 23.676261210254896 26-27 18.67458512822437 24.71823446417649 26.33334191332694 30.273838494272198 28-29 24.575159435186293 31.67121406327847 26.050267564919288 17.703358936615953 30-31 17.558015299146568 26.138143423173137 32.23870429299253 24.065136984687765 32-33 17.37956961006983 32.433542859685694 26.408031484795526 23.778856045448954 34-35 17.771351750362324 32.376369563301594 33.19869650992036 16.65358217641572 36-37 17.731334805602067 26.474061079665677 32.98743234529617 22.807171769436085 38-39 24.89076506283824 32.46791392436376 25.68233048202544 16.958990530772564 40-41 18.2297415444163 38.455247771310304 25.63714087273368 17.67786981153971 42-43 18.248839301373295 32.05354003265051 32.47381066019124 17.22381000578495 44-45 17.71233146692718 25.641043819007937 32.9185008705254 23.72812384353949 46-47 24.41520576267238 25.550993813180835 26.439937333424663 23.593863090722127 48-49 18.348115825339473 32.41699661369154 32.00200558636734 17.23288197460165 50-51 24.86146983922594 25.755915773662245 31.505453242982746 17.877161144129065 52-53 18.170769455955504 25.691516083694587 25.37100487125562 30.766709589094287 54-55 24.86652803841017 25.797278806946245 31.74131160218162 17.59488155246196 56-57 18.351513461890008 25.743184196943975 31.75325023788613 24.152052103279882 58-59 18.273756272546866 25.804619177354237 32.121122680905565 23.800501869193333 60-61 24.403128460227617 26.09620228975995 31.88221548803334 17.618453761979094 62-63 17.85765547943054 26.18846569313514 31.996563467438232 23.95731535999609 64-65 24.632928280152015 26.101793225244503 31.77387204112163 17.491406453481854 66-67 18.76820414361022 38.21787303721387 25.19377667664096 17.82014614253495 68-69 18.819093887844186 38.4018036727909 25.13591900488222 17.643183434482697 70-71 18.314688206377728 39.11187741544847 25.23675498982254 17.336679388351264 72-73 18.173516629208702 38.945261563112346 25.0832264858795 17.797995321799444 74-75 18.900381126135507 37.66148176160752 25.0024242375323 18.435712874724675 76-77 19.257249978976244 36.029638972714274 25.62777051414351 19.08534053416598 78-79 18.97990482997632 35.06307601811169 26.49304014529315 19.46397900661884 80-81 18.45300654373228 35.03865640366526 26.38397167334826 20.1243653792542 82-83 17.922203307456655 34.5927455136086 26.654600868877047 20.830450310057703 84-85 18.282928372184337 33.88865772489182 26.927685054656802 20.900728848267043 86-87 18.376266564868846 33.617571031248296 27.56695206413263 20.439210339750232 88-89 18.677386910995278 33.84035403245501 27.249572135159806 20.232686921389906 90-91 19.009219002576348 33.194081164744404 26.8386630919217 20.958036740757546 92-93 18.62621135350907 33.668984768938046 26.577088353597638 21.127715523955253 94-95 18.369428955345036 33.866613321215716 26.433496289287962 21.33046143415129 96-97 18.820376785601898 34.21482601919978 26.131419775959248 20.833377419239074 98-99 19.33000435210848 33.117833064462474 26.056935177580204 21.49522740584884 100-101 19.369466063174688 33.35639185791935 25.5801171381775 21.694024940728465 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 70.0 2 109.5 3 286.0 4 315.0 5 196.0 6 172.5 7 175.0 8 176.5 9 164.0 10 150.5 11 153.5 12 154.0 13 159.0 14 160.5 15 176.0 16 206.5 17 248.5 18 287.5 19 357.0 20 530.5 21 776.5 22 1183.0 23 1803.0 24 2563.0 25 3645.0 26 5224.0 27 7421.0 28 10075.0 29 13076.0 30 16921.0 31 21880.0 32 30034.0 33 47902.0 34 78443.5 35 129159.5 36 196112.5 37 273939.0 38 354325.0 39 393800.0 40 393025.5 41 357804.0 42 312223.5 43 299599.5 44 280143.5 45 249781.5 46 237613.0 47 210550.0 48 159691.0 49 116282.5 50 87927.0 51 70600.5 52 57459.5 53 44744.5 54 33848.5 55 24180.5 56 16417.0 57 11338.5 58 8232.5 59 6008.0 60 4363.0 61 3246.5 62 2276.5 63 1476.0 64 947.5 65 587.0 66 397.0 67 293.0 68 195.5 69 121.0 70 90.0 71 71.5 72 50.0 73 32.0 74 31.0 75 22.5 76 13.5 77 15.0 78 11.0 79 5.5 80 4.5 81 4.0 82 3.5 83 1.5 84 1.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15282568274208452 2 0.0 3 0.0048207930182701504 4 0.006238673317761372 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.544062920728711E-5 14-15 0.0 16-17 2.1813543069095704E-5 18-19 0.0 20-21 0.0 22-23 1.636015730182178E-4 24-25 2.1813543069095704E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.180078650910889E-4 40-41 0.0 42-43 0.0 44-45 1.636015730182178E-4 46-47 0.0010034229811784025 48-49 0.002454023595273267 50-51 3.8173700370917483E-4 52-53 0.0015051344717676037 54-55 0.002301328793789597 56-57 0.003970064838575419 58-59 0.005344318051928448 60-61 0.001570575100974891 62-63 7.852875504874455E-4 64-65 0.0011779313257311683 66-67 0.007820155190270812 68-69 0.007154842126663392 70-71 0.008779951085311023 72-73 0.006118698830881346 74-75 0.008561815654620065 76-77 0.004548123729906455 78-79 0.0042863612130773065 80-81 0.0016142021871130823 82-83 0.0 84-85 4.47177632916462E-4 86-87 0.001265185498007551 88-89 0.004548123729906455 90-91 0.006500435834590521 92-93 0.008420027624670944 94-95 0.007623833302648949 96-97 0.009914255324903998 98-99 0.007045774411317913 100-101 0.006980333782110626 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4584308.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.999864428225166 #Duplication Level Percentage of deduplicated Percentage of total 1 88.58069600927332 30.1173165527312 2 4.69870806935977 3.195108746920796 3 1.8888893755481444 1.9266594806565545 4 0.9366684055763989 1.2738639521519757 5 0.6377059671904552 1.0840958214772842 6 0.5678341112988963 1.1583769681130516 7 0.2916149809410306 0.6940408872064155 8 0.24202590144081576 0.6583078269685378 9 0.1765036474667292 0.5400990076460438 >10 1.506834692505328 10.277133362488044 >50 0.21049394390839277 5.094617210528578 >100 0.2100728634472807 14.5869074862031 >500 0.03547276608903224 8.472681086638184 >1k 0.016091674097888326 8.547836647284974 >5k 3.229932217710552E-4 0.765623944571366 >10k+ 6.459863497303163E-5 11.607331018413863 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 528484 11.528108495327976 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8651 0.18870896109074695 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 6936 0.15129873472724784 TruSeq Adapter, Index 13 (97% over 38bp) TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6852 0.14946639710944376 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6378 0.13912677769469242 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6042 0.13179742722347626 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 4778 0.10422510878413929 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4741 0.10341800769058274 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006129605602415894 0.0 0.0 2 0.006696757722212382 0.0 0.0 3 0.007285723385077966 0.0 0.0 4 0.007809248418736263 0.0 0.0 5 0.008747230770707378 0.0 0.0 6 0.009358009976642058 0.0 0.0 7 0.010448687130096843 0.0 0.0 8 0.011670245541966204 0.0 0.0 9 0.012826363324628276 0.0 2.1813543069095708E-5 10-11 0.018879621526302334 0.0 2.1813543069095708E-5 12-13 0.023678601001503388 0.0 2.1813543069095708E-5 14-15 0.02882659716580998 0.0 2.1813543069095708E-5 16-17 0.03285119586205813 0.0 2.1813543069095708E-5 18-19 0.03744294667810278 0.0 2.1813543069095708E-5 20-21 0.043125374647602216 0.0 3.2720314603643563E-5 22-23 0.04889505678937803 0.0 4.3627086138191416E-5 24-25 0.054959221762586635 0.0 4.3627086138191416E-5 26-27 0.0617214201140063 0.0 4.3627086138191416E-5 28-29 0.0690398638136879 0.0 4.3627086138191416E-5 30-31 0.10616651411728881 0.0 4.3627086138191416E-5 32-33 0.11581900692536365 0.0 4.3627086138191416E-5 34-35 0.1237155095163763 0.0 4.3627086138191416E-5 36-37 0.1315792917927853 0.0 4.3627086138191416E-5 38-39 0.14063191216646004 0.0 4.3627086138191416E-5 40-41 0.14974997316934202 0.0 4.3627086138191416E-5 42-43 0.16014412644176612 0.0 4.3627086138191416E-5 44-45 0.17110543183398674 0.0 4.3627086138191416E-5 46-47 0.18321194823733483 0.0 4.3627086138191416E-5 48-49 0.19559113392904665 0.0 4.3627086138191416E-5 50-51 0.2090719035457478 0.0 4.3627086138191416E-5 52-53 0.2240687144057511 0.0 5.453385767273927E-5 54-55 0.23974174510089635 0.0 8.725417227638282E-5 56-57 0.25600374145890725 0.0 1.0906771534547854E-4 58-59 0.27286561025131817 0.0 1.0906771534547854E-4 60-61 0.2908072494256494 0.0 1.0906771534547854E-4 62-63 0.3111810986521848 0.0 1.0906771534547854E-4 64-65 0.332427489601484 0.0 1.0906771534547854E-4 66-67 0.3568368442958021 0.0 1.0906771534547854E-4 68-69 0.38289312149183696 0.0 1.0906771534547854E-4 70-71 0.4108798972494867 0.0 1.3088125841457423E-4 72-73 0.4416478997484462 0.0 1.4178802994912207E-4 74-75 0.47495718001495535 0.0 1.7450834455276566E-4 76-77 0.5128473043259746 0.0 1.9632188762186135E-4 78-79 0.5543584767864638 0.0 1.9632188762186135E-4 80-81 0.5994579770818191 0.0 1.9632188762186135E-4 82-83 0.6524757935112562 0.0 1.9632188762186135E-4 84-85 0.7105543519327235 0.0 1.9632188762186135E-4 86-87 0.7746425414697267 2.1813543069095708E-5 1.9632188762186135E-4 88-89 0.8442604641747457 2.1813543069095708E-5 1.9632188762186135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 65125 0.0 87.47804 1 TCGGAAG 65250 0.0 87.05396 3 CGGAAGA 65325 0.0 86.59739 4 ATCGGAA 65780 0.0 86.432 2 GAAGAGC 65710 0.0 86.037254 6 GGAAGAG 68005 0.0 83.294334 5 AGAGCAC 82300 0.0 68.792 8 GAGCACA 82390 0.0 68.70533 9 AAGAGCA 82965 0.0 68.120384 7 CGACGCG 30 1.2905616E-4 63.44283 1 TCGGACG 205 0.0 48.6645 3 GATCGGC 365 0.0 48.233932 1 GACGCGT 50 2.8295623E-5 47.4965 7 TTGTGTT 39095 0.0 47.442158 1 GCCGTCT 61590 0.0 46.11836 50-51 ATGCCGT 61450 0.0 46.110092 48-49 CGTCTTC 61655 0.0 46.055084 52-53 TCGTATG 61955 0.0 45.967575 44-45 TCTCGTA 60880 0.0 45.948368 42-43 AATCTCG 60380 0.0 45.92324 40-41 >>END_MODULE